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The map label for this gene is bluB [H]

Identifier: 94311151

GI number: 94311151

Start: 2426629

End: 2427312

Strand: Reverse

Name: bluB [H]

Synonym: Rmet_2213

Alternate gene names: 94311151

Gene position: 2427312-2426629 (Counterclockwise)

Preceding gene: 94311152

Following gene: 94311148

Centisome position: 61.79

GC content: 67.69

Gene sequence:

>684_bases
ATGTCAGGCCCGCACGTTGAAGCTTCCGAGCGCGAAGCGCTCTACCGCATCATGGCGTTGCGGCGCGATTGCCGTCATTT
CACGCCCGGGCCGTTGCTTGCCGATGACCAGTTGGAACGGCTGCTGGATGCGGCGCACCAGGCGCCCTCGGTGGGCCTGA
TGCAGCCGTGGCGCTTCATGCGCCTGAGCACGCCAGCCTGGCGCGAACACCTGATTCCATTCGTGGAAGCCGAGCGACAG
GCCACGGCACAGGCCCTGGGGGAACGGGCCGCGGAATTCCTGCGGATCAAGGTGGAGGGTATTCGCGATTGCGCTGAACT
GCTGGCCGTGGTGCTGGCGCCGGATGACGGTACCGTCTTCGGACGGCGCAGCATGCCGCGTGAGATGGCGTGGGCGTCCG
CCGCGTGCGCCGTGCAGAATCTCTGGCTGGCGGCTCGCGCCGAAAACCTGGGGCTAGGCTGGGTCTCGATGTTCGACCCC
GAAGCGCTGGCACGCGAACTTCAGCTTCCCGAAGGCGCCACGCCGTTTGGGCTGCTGTGCCTGGGGCCCGTGCCGGCATT
CTATGAAAAGCCGATGCTCGAGCAGATTGGCTGGCGGCAGCGCCGCCCGCTGCAGGAGATGTTGTGGGCGTCCCCGGACC
TGCCGCCTGCCTCTGGTGGCGGGGAACAGGCCGGAGGCGACTGA

Upstream 100 bases:

>100_bases
GCGACTTGCGCTTGTCGCTGCTGTTTGGTATTTGTCGGGCGTCCAGGCGTTGCATTCCGTTTCGGGATGACGCGGACATC
GACAACAAGGAAGAAAAAGC

Downstream 100 bases:

>100_bases
CAGCGGGAAGCGACCGCTTGCGGCATGGCGTGGCTACCGCAAAGGCTCCCGACCGTGAGAACGTCGCGGACATCGGTATT
GTTGACGCTCCGTCTGGCCG

Product: cob(II)yrinic acid a,c-diamide reductase (BluB-like)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSGPHVEASEREALYRIMALRRDCRHFTPGPLLADDQLERLLDAAHQAPSVGLMQPWRFMRLSTPAWREHLIPFVEAERQ
ATAQALGERAAEFLRIKVEGIRDCAELLAVVLAPDDGTVFGRRSMPREMAWASAACAVQNLWLAARAENLGLGWVSMFDP
EALARELQLPEGATPFGLLCLGPVPAFYEKPMLEQIGWRQRRPLQEMLWASPDLPPASGGGEQAGGD

Sequences:

>Translated_227_residues
MSGPHVEASEREALYRIMALRRDCRHFTPGPLLADDQLERLLDAAHQAPSVGLMQPWRFMRLSTPAWREHLIPFVEAERQ
ATAQALGERAAEFLRIKVEGIRDCAELLAVVLAPDDGTVFGRRSMPREMAWASAACAVQNLWLAARAENLGLGWVSMFDP
EALARELQLPEGATPFGLLCLGPVPAFYEKPMLEQIGWRQRRPLQEMLWASPDLPPASGGGEQAGGD
>Mature_226_residues
SGPHVEASEREALYRIMALRRDCRHFTPGPLLADDQLERLLDAAHQAPSVGLMQPWRFMRLSTPAWREHLIPFVEAERQA
TAQALGERAAEFLRIKVEGIRDCAELLAVVLAPDDGTVFGRRSMPREMAWASAACAVQNLWLAARAENLGLGWVSMFDPE
ALARELQLPEGATPFGLLCLGPVPAFYEKPMLEQIGWRQRRPLQEMLWASPDLPPASGGGEQAGGD

Specific function: Maybe catalyzes the reduction of Co(2+) to Co(+) in cob(II)yrinic acid A,C-diamide to allow adenosylation to procede [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012825
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.16.8.1 [H]

Molecular weight: Translated: 25149; Mature: 25018

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPHVEASEREALYRIMALRRDCRHFTPGPLLADDQLERLLDAAHQAPSVGLMQPWRFM
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
RLSTPAWREHLIPFVEAERQATAQALGERAAEFLRIKVEGIRDCAELLAVVLAPDDGTVF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
GRRSMPREMAWASAACAVQNLWLAARAENLGLGWVSMFDPEALARELQLPEGATPFGLLC
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHCCCCCCCCCCEEE
LGPVPAFYEKPMLEQIGWRQRRPLQEMLWASPDLPPASGGGEQAGGD
ECCCCHHHHCHHHHHCCCHHCCCHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SGPHVEASEREALYRIMALRRDCRHFTPGPLLADDQLERLLDAAHQAPSVGLMQPWRFM
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
RLSTPAWREHLIPFVEAERQATAQALGERAAEFLRIKVEGIRDCAELLAVVLAPDDGTVF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
GRRSMPREMAWASAACAVQNLWLAARAENLGLGWVSMFDPEALARELQLPEGATPFGLLC
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHCCCCCCCCCCEEE
LGPVPAFYEKPMLEQIGWRQRRPLQEMLWASPDLPPASGGGEQAGGD
ECCCCHHHHCHHHHHCCCHHCCCHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7635831; 9256491 [H]