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The map label for this gene is prpE [H]

Identifier: 94311141

GI number: 94311141

Start: 2414268

End: 2416157

Strand: Reverse

Name: prpE [H]

Synonym: Rmet_2203

Alternate gene names: 94311141

Gene position: 2416157-2414268 (Counterclockwise)

Preceding gene: 94311142

Following gene: 94311140

Centisome position: 61.51

GC content: 65.66

Gene sequence:

>1890_bases
ATGACGGCAAGCCGCGCACTGCACGCGCGCTCCCTTTCCGACCCGGCAGGATTCTGGGCCGAACAAGCCGCACGTATCGA
TTGGGAGACCCCCTTCGAACGCGTGCTCGACGACAGTCGCCCACCTTTCGCCCGCTGGTTCGTCGGCGGGCGCACCAACA
TCTGCCATAACGCCATCGACCGGCACTTGTCCGCGCGTGCCGACCAGCGGGCGCTGGTCTATGTCTCGACCGAGACCGAT
ACCGAACGCACCTACACTTACCGCGAGTTGCATGACGAAGTCACGCGCATGGCGGCGACGCTGCAGGGGCTGGGCGTGCA
GCGCGGCGACCGGGTGCTGATCTACATGCCGATGATTCCGGAGGCGATGTTCGCGATGCTGGCCTGCACGCGCATCGGCG
CCGTGCATTCGGTGGTGTTCGGTGGGTTTGCCTCGGTCAGCCTGGCGGCCCGTATCGACGACGCCAAGCCACGAGTGATC
GTGAGCGCCGATGCCGGTTCGCGCGCGGGCAAGGTGGTGCCGTACAAGCCGCTGCTTGATGAGGCGATCCGGCTGGCTGG
CCATCATCCCGAAAAGGTGCTGCTGGTGGACCGCAAGCTGGCAGAGATGCCGCTGGTCAGCGGCCGCGACCAGGACTACG
CGGCCTGGCGCGACAAGGTGGCCGGACAAGCGGTGCCATGCGTCTGGATGGAATCGAGCGAGCCATCCTACGTGCTCTAC
ACGTCGGGCACCACGGGCAAGCCAAAGGGCGTGCAGCGAGACACCGGTGGCTACGCGGTGGCACTGGCCACGTCGATGGA
GAACATCTTCTGTGGCAAGCCGGGCGACACGATGTTCACCGCGTCCGATATCGGATGGGTGGTGGGCCATAGCTACATCG
TCTACGGGCCGCTGCTGGCCGGCATGACCACGGTCATGTACGAGGGCACGCCGATTCGTCCTGATGGCGCGATCCTGTGG
AAGATCGTCGAGCAGTACGGCGTCAACATCATGTTCAGCGCGCCTACGGCGATCCGTGTACTCAAGAAGCAGGACCCGGC
GCTGCTCAAGCGTCACGACCTGTCCAGCCTGCGGCTGTTGTTCCTGGCGGGCGAGCCACTGGACGAACCTACCGCGCGCT
GGATTCAGGGCGGCATCGGCAAGCCCGTGGTCGACAACTACTGGCAGACCGAATCGGGCTGGCCGATTATCGCTATCCAG
CGCGGCGTGGAAGCGCTGCCACCCAAACTGGGATCTCCCGGGGTGCCGGCCTACGGTTACGACCTGCGCATCGTCGATGA
GGTCACCGGCGAGGAGTGTCCGCCAAACACCAAGGGCGTGGTGGCGATCGACTATCCGCTGCCACCGGGCTGCATGTCGA
CGGTCTGGGGCGACGACGACCGCTTCATTCGCACGTACTGGTCGGCGGTGCCGAATCGTCAGTGCTATTCCACATTCGAC
TGGGGTATCCGCGACGAGGATGGCTACGTCTTCATCCTCGGCCGCACCGACGATGTGATCAACGTGGCAGGCCACCGACT
GGGCACCCGAGAGATCGAGGAAAGCCTGTCGTCGCACCCGGGCGTGGCCGAGGTGGCCGTGGTCGGCGTGCAGGATGCGC
TGAAGGGCCAGGTCGCCATGGGCTTCTGCATTGCGCGTGACGCCGGGCGCACGGCAACTGAAGCGGACCGTATGGCATTC
GAGGGGGAGTTGATGAAGACGGTGGAGCAGCAGCTCGGCGCGGTGGCGCGGCCGGCGCGCGTGTTCTTCGTCAATGCGCT
GCCCAAGACACGCTCGGGCAAATTGCTGCGCCGCGCCATTCAGGCGGTGGCGGAAGGCCGTGATCCGGGTGACCTGACCA
CGATCGAGGACCCGACGGCGCTGGAGCAGGTGAAGGAGTCGCTGGCCTGA

Upstream 100 bases:

>100_bases
GCGTCGTGCAACCAGTAGGGCAGTCGGCAGGTTTCCGCCTGTCGAACTGTCCCAGCCACCAACACCGCATTGATCATCCA
TATAGCGGAGGAGACATACC

Downstream 100 bases:

>100_bases
CGCAGGCATAGAAGGGCAGGGAGCCCGGCACTTGCGGGCTCCCTGAAAAACCCCTTGCCAGATCTCGGCATGCCGCCTAA
TATTTTAAGCATAAAATATT

Product: propionyl-CoA synthetase

Products: NA

Alternate protein names: Propionyl-CoA synthetase [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MTASRALHARSLSDPAGFWAEQAARIDWETPFERVLDDSRPPFARWFVGGRTNICHNAIDRHLSARADQRALVYVSTETD
TERTYTYRELHDEVTRMAATLQGLGVQRGDRVLIYMPMIPEAMFAMLACTRIGAVHSVVFGGFASVSLAARIDDAKPRVI
VSADAGSRAGKVVPYKPLLDEAIRLAGHHPEKVLLVDRKLAEMPLVSGRDQDYAAWRDKVAGQAVPCVWMESSEPSYVLY
TSGTTGKPKGVQRDTGGYAVALATSMENIFCGKPGDTMFTASDIGWVVGHSYIVYGPLLAGMTTVMYEGTPIRPDGAILW
KIVEQYGVNIMFSAPTAIRVLKKQDPALLKRHDLSSLRLLFLAGEPLDEPTARWIQGGIGKPVVDNYWQTESGWPIIAIQ
RGVEALPPKLGSPGVPAYGYDLRIVDEVTGEECPPNTKGVVAIDYPLPPGCMSTVWGDDDRFIRTYWSAVPNRQCYSTFD
WGIRDEDGYVFILGRTDDVINVAGHRLGTREIEESLSSHPGVAEVAVVGVQDALKGQVAMGFCIARDAGRTATEADRMAF
EGELMKTVEQQLGAVARPARVFFVNALPKTRSGKLLRRAIQAVAEGRDPGDLTTIEDPTALEQVKESLA

Sequences:

>Translated_629_residues
MTASRALHARSLSDPAGFWAEQAARIDWETPFERVLDDSRPPFARWFVGGRTNICHNAIDRHLSARADQRALVYVSTETD
TERTYTYRELHDEVTRMAATLQGLGVQRGDRVLIYMPMIPEAMFAMLACTRIGAVHSVVFGGFASVSLAARIDDAKPRVI
VSADAGSRAGKVVPYKPLLDEAIRLAGHHPEKVLLVDRKLAEMPLVSGRDQDYAAWRDKVAGQAVPCVWMESSEPSYVLY
TSGTTGKPKGVQRDTGGYAVALATSMENIFCGKPGDTMFTASDIGWVVGHSYIVYGPLLAGMTTVMYEGTPIRPDGAILW
KIVEQYGVNIMFSAPTAIRVLKKQDPALLKRHDLSSLRLLFLAGEPLDEPTARWIQGGIGKPVVDNYWQTESGWPIIAIQ
RGVEALPPKLGSPGVPAYGYDLRIVDEVTGEECPPNTKGVVAIDYPLPPGCMSTVWGDDDRFIRTYWSAVPNRQCYSTFD
WGIRDEDGYVFILGRTDDVINVAGHRLGTREIEESLSSHPGVAEVAVVGVQDALKGQVAMGFCIARDAGRTATEADRMAF
EGELMKTVEQQLGAVARPARVFFVNALPKTRSGKLLRRAIQAVAEGRDPGDLTTIEDPTALEQVKESLA
>Mature_628_residues
TASRALHARSLSDPAGFWAEQAARIDWETPFERVLDDSRPPFARWFVGGRTNICHNAIDRHLSARADQRALVYVSTETDT
ERTYTYRELHDEVTRMAATLQGLGVQRGDRVLIYMPMIPEAMFAMLACTRIGAVHSVVFGGFASVSLAARIDDAKPRVIV
SADAGSRAGKVVPYKPLLDEAIRLAGHHPEKVLLVDRKLAEMPLVSGRDQDYAAWRDKVAGQAVPCVWMESSEPSYVLYT
SGTTGKPKGVQRDTGGYAVALATSMENIFCGKPGDTMFTASDIGWVVGHSYIVYGPLLAGMTTVMYEGTPIRPDGAILWK
IVEQYGVNIMFSAPTAIRVLKKQDPALLKRHDLSSLRLLFLAGEPLDEPTARWIQGGIGKPVVDNYWQTESGWPIIAIQR
GVEALPPKLGSPGVPAYGYDLRIVDEVTGEECPPNTKGVVAIDYPLPPGCMSTVWGDDDRFIRTYWSAVPNRQCYSTFDW
GIRDEDGYVFILGRTDDVINVAGHRLGTREIEESLSSHPGVAEVAVVGVQDALKGQVAMGFCIARDAGRTATEADRMAFE
GELMKTVEQQLGAVARPARVFFVNALPKTRSGKLLRRAIQAVAEGRDPGDLTTIEDPTALEQVKESLA

Specific function: Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=628, Percent_Identity=44.5859872611465, Blast_Score=535, Evalue=1e-152,
Organism=Homo sapiens, GI28416953, Length=634, Percent_Identity=38.801261829653, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI8923896, Length=657, Percent_Identity=36.6818873668189, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI253970489, Length=670, Percent_Identity=36.2686567164179, Blast_Score=391, Evalue=1e-109,
Organism=Homo sapiens, GI122937307, Length=546, Percent_Identity=29.6703296703297, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI38505220, Length=577, Percent_Identity=26.3431542461005, Blast_Score=178, Evalue=2e-44,
Organism=Homo sapiens, GI42544132, Length=606, Percent_Identity=24.9174917491749, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI115511026, Length=553, Percent_Identity=27.4864376130199, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI58082049, Length=527, Percent_Identity=28.0834914611006, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI157311624, Length=529, Percent_Identity=28.3553875236295, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI157311622, Length=529, Percent_Identity=28.3553875236295, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI31982927, Length=652, Percent_Identity=24.079754601227, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI42544134, Length=351, Percent_Identity=23.9316239316239, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI195947378, Length=487, Percent_Identity=22.5872689938398, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI187761345, Length=572, Percent_Identity=23.2517482517483, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI187761343, Length=572, Percent_Identity=23.2517482517483, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1786529, Length=622, Percent_Identity=64.7909967845659, Blast_Score=825, Evalue=0.0,
Organism=Escherichia coli, GI1790505, Length=633, Percent_Identity=39.4944707740916, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI1788107, Length=593, Percent_Identity=24.283305227656, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI221142682, Length=533, Percent_Identity=22.3264540337711, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI145693145, Length=553, Percent_Identity=22.7848101265823, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786810, Length=537, Percent_Identity=22.3463687150838, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786801, Length=545, Percent_Identity=23.302752293578, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=641, Percent_Identity=36.1934477379095, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71982997, Length=641, Percent_Identity=36.1934477379095, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17569507, Length=643, Percent_Identity=22.706065318818, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17557194, Length=568, Percent_Identity=23.943661971831, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=568, Percent_Identity=23.7676056338028, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17531443, Length=566, Percent_Identity=22.791519434629, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17559526, Length=582, Percent_Identity=23.8831615120275, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=606, Percent_Identity=21.2871287128713, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=552, Percent_Identity=22.8260869565217, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71996755, Length=566, Percent_Identity=20.3180212014134, Blast_Score=86, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=536, Percent_Identity=21.8283582089552, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319264, Length=641, Percent_Identity=38.8455538221529, Blast_Score=451, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6323182, Length=636, Percent_Identity=38.6792452830189, Blast_Score=432, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319699, Length=379, Percent_Identity=25.3298153034301, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21355225, Length=631, Percent_Identity=41.838351822504, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24667955, Length=649, Percent_Identity=37.7503852080123, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI62472339, Length=565, Percent_Identity=39.1150442477876, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24667959, Length=529, Percent_Identity=38.3742911153119, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI21355181, Length=533, Percent_Identity=24.765478424015, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI18859661, Length=549, Percent_Identity=22.4043715846995, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI20130357, Length=379, Percent_Identity=27.7044854881267, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24656500, Length=533, Percent_Identity=24.765478424015, Blast_Score=73, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21356947, Length=374, Percent_Identity=23.5294117647059, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR012694 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.17 [H]

Molecular weight: Translated: 68869; Mature: 68738

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASRALHARSLSDPAGFWAEQAARIDWETPFERVLDDSRPPFARWFVGGRTNICHNAID
CCCCHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCCHHEEEECCCCHHHHHHHH
RHLSARADQRALVYVSTETDTERTYTYRELHDEVTRMAATLQGLGVQRGDRVLIYMPMIP
HHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCH
EAMFAMLACTRIGAVHSVVFGGFASVSLAARIDDAKPRVIVSADAGSRAGKVVPYKPLLD
HHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHH
EAIRLAGHHPEKVLLVDRKLAEMPLVSGRDQDYAAWRDKVAGQAVPCVWMESSEPSYVLY
HHHHHHCCCCCEEEEECCHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCEEEE
TSGTTGKPKGVQRDTGGYAVALATSMENIFCGKPGDTMFTASDIGWVVGHSYIVYGPLLA
ECCCCCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCEEECCCCCHHHCCCHHEEHHHHH
GMTTVMYEGTPIRPDGAILWKIVEQYGVNIMFSAPTAIRVLKKQDPALLKRHDLSSLRLL
HHHHHEECCCCCCCCCHHHHHHHHHHCCEEEEECCHHHHHHHCCCCHHHHHCCCCCEEEE
FLAGEPLDEPTARWIQGGIGKPVVDNYWQTESGWPIIAIQRGVEALPPKLGSPGVPAYGY
EEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHCCCCCCCCCCCCCCC
DLRIVDEVTGEECPPNTKGVVAIDYPLPPGCMSTVWGDDDRFIRTYWSAVPNRQCYSTFD
CEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCCHHCC
WGIRDEDGYVFILGRTDDVINVAGHRLGTREIEESLSSHPGVAEVAVVGVQDALKGQVAM
CCEECCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEHHHHHCCCHHE
GFCIARDAGRTATEADRMAFEGELMKTVEQQLGAVARPARVFFVNALPKTRSGKLLRRAI
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
QAVAEGRDPGDLTTIEDPTALEQVKESLA
HHHHCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TASRALHARSLSDPAGFWAEQAARIDWETPFERVLDDSRPPFARWFVGGRTNICHNAID
CCCHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCCHHEEEECCCCHHHHHHHH
RHLSARADQRALVYVSTETDTERTYTYRELHDEVTRMAATLQGLGVQRGDRVLIYMPMIP
HHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCH
EAMFAMLACTRIGAVHSVVFGGFASVSLAARIDDAKPRVIVSADAGSRAGKVVPYKPLLD
HHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHH
EAIRLAGHHPEKVLLVDRKLAEMPLVSGRDQDYAAWRDKVAGQAVPCVWMESSEPSYVLY
HHHHHHCCCCCEEEEECCHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCEEEE
TSGTTGKPKGVQRDTGGYAVALATSMENIFCGKPGDTMFTASDIGWVVGHSYIVYGPLLA
ECCCCCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCEEECCCCCHHHCCCHHEEHHHHH
GMTTVMYEGTPIRPDGAILWKIVEQYGVNIMFSAPTAIRVLKKQDPALLKRHDLSSLRLL
HHHHHEECCCCCCCCCHHHHHHHHHHCCEEEEECCHHHHHHHCCCCHHHHHCCCCCEEEE
FLAGEPLDEPTARWIQGGIGKPVVDNYWQTESGWPIIAIQRGVEALPPKLGSPGVPAYGY
EEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHCCCCCCCCCCCCCCC
DLRIVDEVTGEECPPNTKGVVAIDYPLPPGCMSTVWGDDDRFIRTYWSAVPNRQCYSTFD
CEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCCHHCC
WGIRDEDGYVFILGRTDDVINVAGHRLGTREIEESLSSHPGVAEVAVVGVQDALKGQVAM
CCEECCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEHHHHHCCCHHE
GFCIARDAGRTATEADRMAFEGELMKTVEQQLGAVARPARVFFVNALPKTRSGKLLRRAI
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
QAVAEGRDPGDLTTIEDPTALEQVKESLA
HHHHCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9006051; 10411265; 11677609; 10482501 [H]