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The map label for this gene is greA [H]

Identifier: 94311130

GI number: 94311130

Start: 2400127

End: 2400648

Strand: Reverse

Name: greA [H]

Synonym: Rmet_2192

Alternate gene names: 94311130

Gene position: 2400648-2400127 (Counterclockwise)

Preceding gene: 94311131

Following gene: 94311129

Centisome position: 61.11

GC content: 60.54

Gene sequence:

>522_bases
ATGGTTCCAGCTCCGCTGCGGCTCATTTTTTTGTTGAACAAGACAATGAGCACCATTCCGATTACCAAGCGTGGCGCCGA
ACTCCTGAAGGAAGAGCTGCAGCGCCTCAAGGCCGTCGAGCGTCCTGCGGTGATCAACGCCATTTCCGAGGCGCGTGCCC
AGGGCGACCTGTCGGAAAATGCCGAATACGACGCCGCCAAGGAAAAGCAGGCGTTTATCGAAGGCCGCATCCTGGAAGTG
GAAGCGAAGCTTTCCGCCGCCCAGGTGATCGATCCCACCCAGCTGGACACGGACGGTCGCATCGTTTTCGGCGCCACCGT
GGATCTGGAAGAACTGGAATCCGGTAACCCCGTGAGCTACCAGATCGTCGGTGACGACGAGGCGGATCTCGATACCGGCA
AGATCTCCGTGAGCTCGCCGATCGCTCGCGCGCTGATCGGCAAGTTCGAAGGCGACGTAGCAGTCGTGCTGGCCCCGGGC
GGCGAGCGCGAGTACGAAGTCATCACGGTTCGCTACATCTGA

Upstream 100 bases:

>100_bases
GTCGAATTCGCCGATTGCACCGGCCTTGCTGGCCGCTGTGTGATCCCATCTGAAAGCCGCGGTTGAGCGGAAGTGCTGCC
TGGCAGGTGACTGCCGGCGC

Downstream 100 bases:

>100_bases
CGGCGCGGGACAGGCGTGTTCTCCTCGTCTTACTCGAATCTGCCGCCATTGCCGCATCGTCTGTTCCTGCTGCTGACCGT
GATCTGGGCCGGCAGCCTGT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MVPAPLRLIFLLNKTMSTIPITKRGAELLKEELQRLKAVERPAVINAISEARAQGDLSENAEYDAAKEKQAFIEGRILEV
EAKLSAAQVIDPTQLDTDGRIVFGATVDLEELESGNPVSYQIVGDDEADLDTGKISVSSPIARALIGKFEGDVAVVLAPG
GEREYEVITVRYI

Sequences:

>Translated_173_residues
MVPAPLRLIFLLNKTMSTIPITKRGAELLKEELQRLKAVERPAVINAISEARAQGDLSENAEYDAAKEKQAFIEGRILEV
EAKLSAAQVIDPTQLDTDGRIVFGATVDLEELESGNPVSYQIVGDDEADLDTGKISVSSPIARALIGKFEGDVAVVLAPG
GEREYEVITVRYI
>Mature_173_residues
MVPAPLRLIFLLNKTMSTIPITKRGAELLKEELQRLKAVERPAVINAISEARAQGDLSENAEYDAAKEKQAFIEGRILEV
EAKLSAAQVIDPTQLDTDGRIVFGATVDLEELESGNPVSYQIVGDDEADLDTGKISVSSPIARALIGKFEGDVAVVLAPG
GEREYEVITVRYI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=60.126582278481, Blast_Score=186, Evalue=8e-49,
Organism=Escherichia coli, GI87082261, Length=160, Percent_Identity=37.5, Blast_Score=94, Evalue=6e-21,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18766; Mature: 18766

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPAPLRLIFLLNKTMSTIPITKRGAELLKEELQRLKAVERPAVINAISEARAQGDLSEN
CCCCHHHHEEEHHCHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
AEYDAAKEKQAFIEGRILEVEAKLSAAQVIDPTQLDTDGRIVFGATVDLEELESGNPVSY
CCCCCHHHHHHHHCCEEEEEECCCCHHHCCCCCCCCCCCCEEEEEECCHHHHCCCCCEEE
QIVGDDEADLDTGKISVSSPIARALIGKFEGDVAVVLAPGGEREYEVITVRYI
EEECCCCCCCCCCEEEECCHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEEC
>Mature Secondary Structure
MVPAPLRLIFLLNKTMSTIPITKRGAELLKEELQRLKAVERPAVINAISEARAQGDLSEN
CCCCHHHHEEEHHCHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
AEYDAAKEKQAFIEGRILEVEAKLSAAQVIDPTQLDTDGRIVFGATVDLEELESGNPVSY
CCCCCHHHHHHHHCCEEEEEECCCCHHHCCCCCCCCCCCCEEEEEECCHHHHCCCCCEEE
QIVGDDEADLDTGKISVSSPIARALIGKFEGDVAVVLAPGGEREYEVITVRYI
EEECCCCCCCCCCEEEECCHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]