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The map label for this gene is rho [H]

Identifier: 94311073

GI number: 94311073

Start: 2323249

End: 2324511

Strand: Direct

Name: rho [H]

Synonym: Rmet_2135

Alternate gene names: 94311073

Gene position: 2323249-2324511 (Clockwise)

Preceding gene: 94311072

Following gene: 94311074

Centisome position: 59.14

GC content: 61.12

Gene sequence:

>1263_bases
ATGCACCTGACAGAACTCAAATCGCTTCACGTGTCTGCGCTTCTCGAAATGGCGGCAACGCTCGAGATCGACAATGCACA
GCGGATGCGCAAACAGGAACTGATGTTTGCGATCCTCAAGAAGCGCGCCAAGATGGGCGAAACCATCTACGGAGACGGCA
CGCTGGAAGTTCTGCCCGATGGCTTCGGTTTCCTGCGCTCGCCGGAAACTTCCTACTTGGCCAGCACGGACGACATCTAC
ATCAGCCCTTCGCAGATCCGCCGTTTCAACCTGCACACCGGTGACTCGATCGAAGGCGAAGTGCGTACGCCGAAGGACGG
CGAGCGCTATTTCGCGCTGGTGAAGGTGGACAAGGTCAACGGGCAGCCTCCCGAAGCGGTGAAGAACCGCATCATGTTCG
AGAACCTGACGCCGCTGCATCCGAACCGGCCGCTCACGCTCGAACGCGATATCCGCGCCGAGGAAAACATCACCGGCCGC
ATCGTCGACATCATCGCGCCGATCGGCCGCGGCCAGCGCGCGCTGCTGGTGGCATCGCCGAAGTCCGGCAAGACCGTGAT
GCTGCAGCACATCGCCCACGCGATTGCGAACAACCATCCGGAAGCCGACCTGTTCGTGCTGCTAATCGATGAGCGCCCGG
AAGAAGTGACCGAAATGCAGCGCTCGGTGCGCGGCGAAGTGGTGGCGTCGACGTTCGACGAACCTGCGATCCGTCACGTG
CAGGTGGCCGAAATGGTCATCGAGAAAGCCAAGCGGCTGGTGGAACTGAAGCGCGACGTGGTGATCCTGCTGGACTCGAT
CACGCGTCTGGCCCGCGCCTACAACACCGTGGTGCCGGCATCGGGCAAGGTACTGACCGGTGGTGTGGATGCCAACGCGC
TGCAGCGTCCGAAGCGTTTCTTCGGCGCCGCGCGTAACCTGGAGGAAGGCGGTTCGCTGACCATTATCGCCACGGCGCTG
ATCGAAACCGGCAGCCGCATGGACGACGTGATCTACGAAGAGTTCAAGGGCACCGGCAACATGGAAGTGCACCTCGAGCG
CCGCCTGGCCGAGAAGCGCGTCTACCCAGCCATCAACCTGAACAAGTCGGGCACACGCCGCGAGGAACTGCTGATCAAGC
CGGACATCCTCCAGAAGATCTGGATCCTGCGCAAGTTCATCTCCGACATGGACGAAGTGCAGGCGATGGAATTCATCCTG
GACAAGATGAAGCCGACGAAGAACAACGCGGAATTCTTCGACCTGATGCGCCGCGGCGGCTGA

Upstream 100 bases:

>100_bases
TGTGCTATGATGCCACCCAACAACTTCCCCGAGCGTTCGCTCATTCTTTTCCCCCTTCTCCTTGTTCTTGCCCGTCCGGG
CACTTCTGTACCCCTCGTCT

Downstream 100 bases:

>100_bases
TGCCACGCTGGTAGTCCATCCTGATAAAAAGGCGTACCCTTGGGTGCGCCTTTTTTCTTTCGCCCCCCCCCTCGCGAGAA
CCATGCTCGGAAAGCTGACC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MHLTELKSLHVSALLEMAATLEIDNAQRMRKQELMFAILKKRAKMGETIYGDGTLEVLPDGFGFLRSPETSYLASTDDIY
ISPSQIRRFNLHTGDSIEGEVRTPKDGERYFALVKVDKVNGQPPEAVKNRIMFENLTPLHPNRPLTLERDIRAEENITGR
IVDIIAPIGRGQRALLVASPKSGKTVMLQHIAHAIANNHPEADLFVLLIDERPEEVTEMQRSVRGEVVASTFDEPAIRHV
QVAEMVIEKAKRLVELKRDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRFFGAARNLEEGGSLTIIATAL
IETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPAINLNKSGTRREELLIKPDILQKIWILRKFISDMDEVQAMEFIL
DKMKPTKNNAEFFDLMRRGG

Sequences:

>Translated_420_residues
MHLTELKSLHVSALLEMAATLEIDNAQRMRKQELMFAILKKRAKMGETIYGDGTLEVLPDGFGFLRSPETSYLASTDDIY
ISPSQIRRFNLHTGDSIEGEVRTPKDGERYFALVKVDKVNGQPPEAVKNRIMFENLTPLHPNRPLTLERDIRAEENITGR
IVDIIAPIGRGQRALLVASPKSGKTVMLQHIAHAIANNHPEADLFVLLIDERPEEVTEMQRSVRGEVVASTFDEPAIRHV
QVAEMVIEKAKRLVELKRDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRFFGAARNLEEGGSLTIIATAL
IETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPAINLNKSGTRREELLIKPDILQKIWILRKFISDMDEVQAMEFIL
DKMKPTKNNAEFFDLMRRGG
>Mature_420_residues
MHLTELKSLHVSALLEMAATLEIDNAQRMRKQELMFAILKKRAKMGETIYGDGTLEVLPDGFGFLRSPETSYLASTDDIY
ISPSQIRRFNLHTGDSIEGEVRTPKDGERYFALVKVDKVNGQPPEAVKNRIMFENLTPLHPNRPLTLERDIRAEENITGR
IVDIIAPIGRGQRALLVASPKSGKTVMLQHIAHAIANNHPEADLFVLLIDERPEEVTEMQRSVRGEVVASTFDEPAIRHV
QVAEMVIEKAKRLVELKRDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRFFGAARNLEEGGSLTIIATAL
IETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPAINLNKSGTRREELLIKPDILQKIWILRKFISDMDEVQAMEFIL
DKMKPTKNNAEFFDLMRRGG

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=69.377990430622, Blast_Score=605, Evalue=1e-174,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47330; Mature: 47330

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLTELKSLHVSALLEMAATLEIDNAQRMRKQELMFAILKKRAKMGETIYGDGTLEVLPD
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEECCC
GFGFLRSPETSYLASTDDIYISPSQIRRFNLHTGDSIEGEVRTPKDGERYFALVKVDKVN
CCCCCCCCCCCEEECCCCEEECHHHHEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCC
GQPPEAVKNRIMFENLTPLHPNRPLTLERDIRAEENITGRIVDIIAPIGRGQRALLVASP
CCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHEEHHCCCCCCEEEEEECC
KSGKTVMLQHIAHAIANNHPEADLFVLLIDERPEEVTEMQRSVRGEVVASTFDEPAIRHV
CCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHCCCCHHHHHH
QVAEMVIEKAKRLVELKRDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHH
FGAARNLEEGGSLTIIATALIETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPAINL
HHHHCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCEEC
NKSGTRREELLIKPDILQKIWILRKFISDMDEVQAMEFILDKMKPTKNNAEFFDLMRRGG
CCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MHLTELKSLHVSALLEMAATLEIDNAQRMRKQELMFAILKKRAKMGETIYGDGTLEVLPD
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEECCC
GFGFLRSPETSYLASTDDIYISPSQIRRFNLHTGDSIEGEVRTPKDGERYFALVKVDKVN
CCCCCCCCCCCEEECCCCEEECHHHHEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCC
GQPPEAVKNRIMFENLTPLHPNRPLTLERDIRAEENITGRIVDIIAPIGRGQRALLVASP
CCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHEEHHCCCCCCEEEEEECC
KSGKTVMLQHIAHAIANNHPEADLFVLLIDERPEEVTEMQRSVRGEVVASTFDEPAIRHV
CCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHCCCCHHHHHH
QVAEMVIEKAKRLVELKRDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHH
FGAARNLEEGGSLTIIATALIETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPAINL
HHHHCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCEEC
NKSGTRREELLIKPDILQKIWILRKFISDMDEVQAMEFILDKMKPTKNNAEFFDLMRRGG
CCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]