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The map label for this gene is purA

Identifier: 94311034

GI number: 94311034

Start: 2275730

End: 2277073

Strand: Reverse

Name: purA

Synonym: Rmet_2096

Alternate gene names: 94311034

Gene position: 2277073-2275730 (Counterclockwise)

Preceding gene: 94311035

Following gene: 94311033

Centisome position: 57.97

GC content: 63.24

Gene sequence:

>1344_bases
ATGTCCGCATCCGCAGTAGGCCAGGGACGCAATGTCGTCGTGATCGGAACCCAATGGGGTGACGAAGGCAAAGGGAAAAT
CGTCGATTGGCTTACCGATCATGCTAAAGGTGTGGTGCGGTTCCAGGGCGGCCACAACGCTGGCCATACGCTCATCATCG
GCGGCAAGAAGACCATTCTGCGACTGATCCCGTCGGGGATCATGCGCGAAAGCACCGTCTGCTATATCGGCAACGGTGTG
GTGCTGTCGCCTGAGGCGCTGTTCCGCGAAATCGAAGAGCTCGAGTCTGCCGGCCTGCAGGTGCAGAATCGCCTGCGCAT
TTCCGAAGCCGCTACGCTGATCCTGCCGTACCACGTGGCCATCGACAAGGCACGTGAAGCCCGCCGCGGCGCCGCCAAGA
TCGGCACGACGGGACGCGGCATCGGCCCGGCATACGAAGACAAGGTGGCTCGCCGCGCGCTGCGCGTGCAGGACCTGTTC
GATCCGAAGCACTTCGCTGAGCGTCTGCGCGAGAACGTCGAATTCCATAACTTCATGCTGACTCAGTACCTCGGCGCCGA
AGCCGTCGACTATCAGCAGATCCTGGACGAATCGCTGGCATTCGCGCCGCGCCTGAAGCCGATGGTGGCCGATGTGTCGG
CCGAGTTGTACGCTGTGAATGCGGCAGGCAATAACCTGATGTTCGAAGGCGCGCAAGGCACGCTGCTCGACGTGGATCAC
GGCACCTATCCGTTCGTGACCTCGAGCAACTGCGTGGCCGGCGCCGCAGCGGCTGGTGCGGGCGTGGGCCCGGGTCGCCT
GAACTACATCCTTGGCATCACCAAGGCGTACTGCACGCGCGTTGGCGCTGGTCCGTTCCCGAGCGAACTGTACGACAACG
ACAACCCTGCCCGTCAGGATGCCGTGGGCGTGCGCCTGGCCAACGTCGGCAAGGAATTCGGTTCCGTGACCGGCCGTCCG
CGCCGCACTGGCTGGCTCGATGCCGCCGCGCTGAAGCGCTCGGTGCAGATCAACGGTGTGTCCGGTCTGTGCATGACCAA
GCTGGACGTGCTCGATGGTCTGGAAACGATCAAGCTGTGCGTAGGCTACGAGCTCGACGGCAAGCGCGTCGATATCCTGC
CGCGTGGCTCGGATGCCGTGGCCGCTTGCGTGCCGGTGTACGAAGAGTTTCCGGGCTGGAACGAATCGACCTTTGGCGTG
AAGACCTGGGATGCGCTGCCCGAAGCGGCACGCGTTTACCTGAAGCGAATCGAGGAAGTGGTCGGCATTCCGATCGACAT
GGTGTCGACCGGTCCGGACCGCGACGAGACGATTCTGCTGCGCCATCCGTACCAGGTCGCCTGA

Upstream 100 bases:

>100_bases
CCGCATTGGACAACAATCGTCTGTCATACGCGTGCCCGAAAACCGGGAACCGTAGAACAACGAGAGTAGAATACGTTTTT
AACCTTCTGACCAATTCAAC

Downstream 100 bases:

>100_bases
CCCGCCTGCATTACCCCGTGAGCATGATCGTGGCGCCACGAGCGCCACGGCCTCACAGATAGTCCTCGGCCCGCGATATG
CGGGCCGATGCTTTATCCAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSASAVGQGRNVVVIGTQWGDEGKGKIVDWLTDHAKGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMRESTVCYIGNGV
VLSPEALFREIEELESAGLQVQNRLRISEAATLILPYHVAIDKAREARRGAAKIGTTGRGIGPAYEDKVARRALRVQDLF
DPKHFAERLRENVEFHNFMLTQYLGAEAVDYQQILDESLAFAPRLKPMVADVSAELYAVNAAGNNLMFEGAQGTLLDVDH
GTYPFVTSSNCVAGAAAAGAGVGPGRLNYILGITKAYCTRVGAGPFPSELYDNDNPARQDAVGVRLANVGKEFGSVTGRP
RRTGWLDAAALKRSVQINGVSGLCMTKLDVLDGLETIKLCVGYELDGKRVDILPRGSDAVAACVPVYEEFPGWNESTFGV
KTWDALPEAARVYLKRIEEVVGIPIDMVSTGPDRDETILLRHPYQVA

Sequences:

>Translated_447_residues
MSASAVGQGRNVVVIGTQWGDEGKGKIVDWLTDHAKGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMRESTVCYIGNGV
VLSPEALFREIEELESAGLQVQNRLRISEAATLILPYHVAIDKAREARRGAAKIGTTGRGIGPAYEDKVARRALRVQDLF
DPKHFAERLRENVEFHNFMLTQYLGAEAVDYQQILDESLAFAPRLKPMVADVSAELYAVNAAGNNLMFEGAQGTLLDVDH
GTYPFVTSSNCVAGAAAAGAGVGPGRLNYILGITKAYCTRVGAGPFPSELYDNDNPARQDAVGVRLANVGKEFGSVTGRP
RRTGWLDAAALKRSVQINGVSGLCMTKLDVLDGLETIKLCVGYELDGKRVDILPRGSDAVAACVPVYEEFPGWNESTFGV
KTWDALPEAARVYLKRIEEVVGIPIDMVSTGPDRDETILLRHPYQVA
>Mature_446_residues
SASAVGQGRNVVVIGTQWGDEGKGKIVDWLTDHAKGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMRESTVCYIGNGVV
LSPEALFREIEELESAGLQVQNRLRISEAATLILPYHVAIDKAREARRGAAKIGTTGRGIGPAYEDKVARRALRVQDLFD
PKHFAERLRENVEFHNFMLTQYLGAEAVDYQQILDESLAFAPRLKPMVADVSAELYAVNAAGNNLMFEGAQGTLLDVDHG
TYPFVTSSNCVAGAAAAGAGVGPGRLNYILGITKAYCTRVGAGPFPSELYDNDNPARQDAVGVRLANVGKEFGSVTGRPR
RTGWLDAAALKRSVQINGVSGLCMTKLDVLDGLETIKLCVGYELDGKRVDILPRGSDAVAACVPVYEEFPGWNESTFGVK
TWDALPEAARVYLKRIEEVVGIPIDMVSTGPDRDETILLRHPYQVA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=444, Percent_Identity=40.7657657657658, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI34577063, Length=442, Percent_Identity=39.1402714932127, Blast_Score=295, Evalue=6e-80,
Organism=Homo sapiens, GI40316944, Length=405, Percent_Identity=39.2592592592593, Blast_Score=286, Evalue=3e-77,
Organism=Escherichia coli, GI1790620, Length=437, Percent_Identity=61.7848970251716, Blast_Score=553, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI25146555, Length=443, Percent_Identity=41.7607223476298, Blast_Score=324, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI25146553, Length=443, Percent_Identity=41.7607223476298, Blast_Score=323, Evalue=8e-89,
Organism=Saccharomyces cerevisiae, GI6324109, Length=437, Percent_Identity=39.3592677345538, Blast_Score=315, Evalue=7e-87,
Organism=Drosophila melanogaster, GI21356033, Length=430, Percent_Identity=39.7674418604651, Blast_Score=305, Evalue=3e-83,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_RALME (Q1LLK1)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584244.1
- ProteinModelPortal:   Q1LLK1
- SMR:   Q1LLK1
- STRING:   Q1LLK1
- GeneID:   4038904
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_2096
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   KRGRLQQ
- PhylomeDB:   Q1LLK1
- ProtClustDB:   PRK01117
- BioCyc:   RMET266264:RMET_2096-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 48262; Mature: 48130

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 21-21 ACT_SITE 49-49 BINDING 137-137 BINDING 151-151 BINDING 232-232 BINDING 247-247 BINDING 319-319 BINDING 321-321

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASAVGQGRNVVVIGTQWGDEGKGKIVDWLTDHAKGVVRFQGGHNAGHTLIIGGKKTIL
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHH
RLIPSGIMRESTVCYIGNGVVLSPEALFREIEELESAGLQVQNRLRISEAATLILPYHVA
HHHHHHHHCCCCEEEECCCEEECHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEEHHHH
IDKAREARRGAAKIGTTGRGIGPAYEDKVARRALRVQDLFDPKHFAERLRENVEFHNFML
HHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHH
TQYLGAEAVDYQQILDESLAFAPRLKPMVADVSAELYAVNAAGNNLMFEGAQGTLLDVDH
HHHHCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCEEEEEECCCCCEEEECCCCCEEECCC
GTYPFVTSSNCVAGAAAAGAGVGPGRLNYILGITKAYCTRVGAGPFPSELYDNDNPARQD
CCCCEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHH
AVGVRLANVGKEFGSVTGRPRRTGWLDAAALKRSVQINGVSGLCMTKLDVLDGLETIKLC
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCEEEECCCCCHHHHHHHHHCCHHHHHHH
VGYELDGKRVDILPRGSDAVAACVPVYEEFPGWNESTFGVKTWDALPEAARVYLKRIEEV
CCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEHHHHCHHHHHHHHHHHHHH
VGIPIDMVSTGPDRDETILLRHPYQVA
HCCCEEEECCCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
SASAVGQGRNVVVIGTQWGDEGKGKIVDWLTDHAKGVVRFQGGHNAGHTLIIGGKKTIL
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHH
RLIPSGIMRESTVCYIGNGVVLSPEALFREIEELESAGLQVQNRLRISEAATLILPYHVA
HHHHHHHHCCCCEEEECCCEEECHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEEHHHH
IDKAREARRGAAKIGTTGRGIGPAYEDKVARRALRVQDLFDPKHFAERLRENVEFHNFML
HHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHH
TQYLGAEAVDYQQILDESLAFAPRLKPMVADVSAELYAVNAAGNNLMFEGAQGTLLDVDH
HHHHCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCEEEEEECCCCCEEEECCCCCEEECCC
GTYPFVTSSNCVAGAAAAGAGVGPGRLNYILGITKAYCTRVGAGPFPSELYDNDNPARQD
CCCCEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHH
AVGVRLANVGKEFGSVTGRPRRTGWLDAAALKRSVQINGVSGLCMTKLDVLDGLETIKLC
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCEEEECCCCCHHHHHHHHHCCHHHHHHH
VGYELDGKRVDILPRGSDAVAACVPVYEEFPGWNESTFGVKTWDALPEAARVYLKRIEEV
CCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEHHHHCHHHHHHHHHHHHHH
VGIPIDMVSTGPDRDETILLRHPYQVA
HCCCEEEECCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA