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The map label for this gene is narH [H]

Identifier: 94311013

GI number: 94311013

Start: 2254147

End: 2255700

Strand: Direct

Name: narH [H]

Synonym: Rmet_2075

Alternate gene names: 94311013

Gene position: 2254147-2255700 (Clockwise)

Preceding gene: 94311012

Following gene: 94311014

Centisome position: 57.39

GC content: 62.16

Gene sequence:

>1554_bases
ATGAAGATTCGTGCCCAGGTAGCCATGGTGCTGAACCTCGACAAGTGCATCGGCTGCCATACCTGCTCCGTGACGTGCAA
GAACGTATGGACCAGCCGTGAAGGTGTCGAGTACGCATGGTTCAACAACGTCGAGACCAAGCCCGGTGTCGGCTTCCCGA
AGGAATGGGAGAACCAGGACAAATGGCAAGGCGGCTGGAAGCGCAATGCCGACGGTAGCCTCACGCCCCGTCAGGGCGGC
AAGACAAAGATCCTGGCGAACATCTTCGCCAACCCGAACCTGCCGCAGATCGACGACTACTACGAGCCGTTCACCTTCGA
CTACGAGCATCTCCAGAATGCCCCGCTGATGCAGACGCCGCCCACGGCGCGTCCCGTATCGGCAATCACCGGCAAGAAGA
TGGAGAAGATCGAATGGGGTCCGAACTGGGAAGACGACCTCGGGGGCGAGTTCGACCAGCGCAGCCGCGACAAGCTTTTT
GAGGACATTCAGAAGGACATGTACTCGACCTTCGAGAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAGCACTGCCT
GAACCCTACCTGCGTGGCGTCCTGCCCCTCCGGCTCGGTCTACAAGCGCGAGGAAGACGGCATCGTCCTGGTCGACCAGG
ACAAGTGCCGCGGCTGGCGCATGTGCGTGTCGGGCTGCCCGTACAAGAAGATCTACTTCAACTGGCAGACCGGCAAGGCC
GAAAAGTGCGTGTTCTGCTATCCGCGTATCGAGGCCGGGCAACCGACGGTCTGTTCGGAAACCTGCGTGGGCCGCATCCG
CTATCTCGGCGTGATGCTCTATGACGCCGACCGCATCGAACAAGCCGCGTCCGTGGCCGACGAGCGCGATCTGTACCAGT
CGCAACTCGACGTCTTCCTCGATCCGCACGATCCTAAGATCCAGGCCGAGGCGCTGCGTCAGGGCATCCCGCAAAGCTGG
CTGGACGCCGCGGTCAAGTCGCCGGTCTACAAGATGGCCTGCGAGTGGAAGGTGGCCTTCCCGCTGCACCCGGAATACCG
CACGCTGCCGATGGTCTGGTACATCCCGCCGCTCTCGCCGATCCAGTCGGCCGCGGAATCGGGCTTCATGGGCATGAACG
GCGTGATCCCGGACGTGAAGAGCCTGCGCATTCCGGTCAAGTACCTCGCAAACCTGCTGACCGCAGGCGATGTGAATCCG
GTGCTGTCCGCGCTGGAACGCATGCTGGCGATGCGCGCATACAAGCGCTCTGAATCGGTGGACGGCCTGCAGGACCTGGC
CGTGCTCGAACAGGTGGGGCTGACGCCCGCGCAGGTCGAGGACATGTACCAGATCATGGCCATCGCCAACTACGAGGACC
GCTTCGTCGTGCCGTCGTCCCACAAGGAAATGGCGGAAGACAGCTTCGACGAGAAGGGAAGCTGCGGTTTCAGCTTCGGC
AACGGCTGCTCGGGTGGCACGTCGGACGGCTCGCTGTTCGGCACGAAGAAGCCCAAGCCAGAAGTGTCGTACGCCGACTT
GCCGCGCTCGCGCCGCAAGGCCATCACCGGTTAA

Upstream 100 bases:

>100_bases
TGATCCTGCGCAAGATGAACAAGGTGGACTGGCTCGACCAGCCGCTACGAGAAGAACGTACCCCTGCAAACGAAGCAGAA
AAGATCGAAGGAGCAAGGTA

Downstream 100 bases:

>100_bases
AGGAAGGAGTTCGAATCATGCCCATTCATCAGGTGCTCAGCACCCTGCTCTGCTACCCCGAACAGGAATGGCTCGACAGC
CTCGAGGAAGCCAACCTCGC

Product: nitrate reductase 1, beta (Fe-S) subunit

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGVGFPKEWENQDKWQGGWKRNADGSLTPRQGG
KTKILANIFANPNLPQIDDYYEPFTFDYEHLQNAPLMQTPPTARPVSAITGKKMEKIEWGPNWEDDLGGEFDQRSRDKLF
EDIQKDMYSTFENTFMMYLPRLCEHCLNPTCVASCPSGSVYKREEDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWQTGKA
EKCVFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFLDPHDPKIQAEALRQGIPQSW
LDAAVKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSPIQSAAESGFMGMNGVIPDVKSLRIPVKYLANLLTAGDVNP
VLSALERMLAMRAYKRSESVDGLQDLAVLEQVGLTPAQVEDMYQIMAIANYEDRFVVPSSHKEMAEDSFDEKGSCGFSFG
NGCSGGTSDGSLFGTKKPKPEVSYADLPRSRRKAITG

Sequences:

>Translated_517_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGVGFPKEWENQDKWQGGWKRNADGSLTPRQGG
KTKILANIFANPNLPQIDDYYEPFTFDYEHLQNAPLMQTPPTARPVSAITGKKMEKIEWGPNWEDDLGGEFDQRSRDKLF
EDIQKDMYSTFENTFMMYLPRLCEHCLNPTCVASCPSGSVYKREEDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWQTGKA
EKCVFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFLDPHDPKIQAEALRQGIPQSW
LDAAVKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSPIQSAAESGFMGMNGVIPDVKSLRIPVKYLANLLTAGDVNP
VLSALERMLAMRAYKRSESVDGLQDLAVLEQVGLTPAQVEDMYQIMAIANYEDRFVVPSSHKEMAEDSFDEKGSCGFSFG
NGCSGGTSDGSLFGTKKPKPEVSYADLPRSRRKAITG
>Mature_517_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGVGFPKEWENQDKWQGGWKRNADGSLTPRQGG
KTKILANIFANPNLPQIDDYYEPFTFDYEHLQNAPLMQTPPTARPVSAITGKKMEKIEWGPNWEDDLGGEFDQRSRDKLF
EDIQKDMYSTFENTFMMYLPRLCEHCLNPTCVASCPSGSVYKREEDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWQTGKA
EKCVFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFLDPHDPKIQAEALRQGIPQSW
LDAAVKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSPIQSAAESGFMGMNGVIPDVKSLRIPVKYLANLLTAGDVNP
VLSALERMLAMRAYKRSESVDGLQDLAVLEQVGLTPAQVEDMYQIMAIANYEDRFVVPSSHKEMAEDSFDEKGSCGFSFG
NGCSGGTSDGSLFGTKKPKPEVSYADLPRSRRKAITG

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=498, Percent_Identity=72.289156626506, Blast_Score=793, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=498, Percent_Identity=69.6787148594377, Blast_Score=779, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=162, Percent_Identity=33.9506172839506, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787122, Length=109, Percent_Identity=41.2844036697248, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=36.6666666666667, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI2367345, Length=101, Percent_Identity=31.6831683168317, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1787749, Length=102, Percent_Identity=32.3529411764706, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789370, Length=97, Percent_Identity=35.0515463917526, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=32.183908045977, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58223; Mature: 58223

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGVGFPKEWENQD
CCCHHEEEEEEEHHHHHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCHHCCCCC
KWQGGWKRNADGSLTPRQGGKTKILANIFANPNLPQIDDYYEPFTFDYEHLQNAPLMQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
PTARPVSAITGKKMEKIEWGPNWEDDLGGEFDQRSRDKLFEDIQKDMYSTFENTFMMYLP
CCCCCHHHHHCCCHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPTCVASCPSGSVYKREEDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWQTGKA
HHHHHHCCCEEEEECCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCVFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHEEE
DPHDPKIQAEALRQGIPQSWLDAAVKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSP
CCCCCCCHHHHHHCCCCHHHHHHHHHCCHHHHEECEEEECCCCCCCCCCCEEEECCCCCH
IQSAAESGFMGMNGVIPDVKSLRIPVKYLANLLTAGDVNPVLSALERMLAMRAYKRSESV
HHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
DGLQDLAVLEQVGLTPAQVEDMYQIMAIANYEDRFVVPSSHKEMAEDSFDEKGSCGFSFG
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCCCCCC
NGCSGGTSDGSLFGTKKPKPEVSYADLPRSRRKAITG
CCCCCCCCCCCEECCCCCCCCCCHHCCCHHHHHCCCC
>Mature Secondary Structure
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGVGFPKEWENQD
CCCHHEEEEEEEHHHHHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCHHCCCCC
KWQGGWKRNADGSLTPRQGGKTKILANIFANPNLPQIDDYYEPFTFDYEHLQNAPLMQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
PTARPVSAITGKKMEKIEWGPNWEDDLGGEFDQRSRDKLFEDIQKDMYSTFENTFMMYLP
CCCCCHHHHHCCCHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPTCVASCPSGSVYKREEDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWQTGKA
HHHHHHCCCEEEEECCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCVFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHEEE
DPHDPKIQAEALRQGIPQSWLDAAVKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSP
CCCCCCCHHHHHHCCCCHHHHHHHHHCCHHHHEECEEEECCCCCCCCCCCEEEECCCCCH
IQSAAESGFMGMNGVIPDVKSLRIPVKYLANLLTAGDVNPVLSALERMLAMRAYKRSESV
HHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
DGLQDLAVLEQVGLTPAQVEDMYQIMAIANYEDRFVVPSSHKEMAEDSFDEKGSCGFSFG
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCCCCCC
NGCSGGTSDGSLFGTKKPKPEVSYADLPRSRRKAITG
CCCCCCCCCCCEECCCCCCCCCCHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]