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The map label for this gene is narK [H]

Identifier: 94311011

GI number: 94311011

Start: 2248975

End: 2250357

Strand: Direct

Name: narK [H]

Synonym: Rmet_2073

Alternate gene names: 94311011

Gene position: 2248975-2250357 (Clockwise)

Preceding gene: 94311010

Following gene: 94311012

Centisome position: 57.25

GC content: 65.87

Gene sequence:

>1383_bases
ATGTCAAGTTCCATACTGACCCGCTGGGAGCCCGAGAACGTGGCCTTCTGGCGCGACCAGGGCGAGCGAATCGCCTATCG
CAACCTCTGGATTTCGATCCCCGCGCTGATGCTGGCGTTCGTGATCTGGATGCTCTGGAGCGTGGTGGCGGTCAACCTCG
ACCGCGCCGGCTTTCAGTTCACCAAGAACCAGCTCTTCTGGCTGACCGCGCTGCCCGCGCTGTCCGGTGCCACACTGCGT
ATCTTCTACTCGTTCCTGGTGCCGATCTTTGGCGGCCGCCGCTTCACGGCCATCTCCACGGCGCTGCTGCTGATTCCGGC
GGTGGGCATGGGCTTCGCGCTACGCGACCCGTCGACCAGCTACCCCACGCTGCTGATCTTGGCGCTGCTGTGCGGCCTGG
GCGGCGCGAACTTCAGTTCGTCGATGGCCAACATCAGCTTCTTCTTCCCGAAAGAAAAGAAGGGCCTGGCCACTGGCCTG
AACGCCGGTATCGGCAACCTGGGCGTCTCCGTGGTGCAGTTCGTCACGCCACTGGTGGTTTCGGTGGCCGTGTTCGGCGC
GCTCGGCGGTGAACCGCAGACCTACAAGGCGGCCGACGCCACGCAACAACTCTGGCTGCAGAACGCCGGTTTCATCTGGG
TGCCGTTTATCATCGTCGCCTCGCTGGCGGCATGGTTCGGCATGCACGACATCGCCGACGCGCGCGCATCGTTCGCCGAA
CAGGCCGTGATCTTCACACGCAAGCACAACTGGCTGATGTGCTGGCTCTATATCGGCACGTTCGGCTCGTTCATCGGCTT
CTCGGCCGGCCTGGCGCTGCTGACCAAGTCGCAGTTCCCCGGCGTCAACCCGACGGCCTACGCCTTCCTCGGACCGCTCG
TTGGCGCACTGACCCGCCCGGTTGGTGGCTGGATCTCGGACAGACTCGGCGGTGCCCGCGTCACGCTCTGGACCTTCATC
GGCATGATCCTCGCGGTCTTCGCCGTGCTGGCCGCCCTGCCCGCCGATGGCGGCGCGGGCAGCTTCCCCGCCTTCCTCGG
CGCCTTCATCGTGCTGTTCGCCCTGACCGGCATCGGCAACGGCTCGACGTTCCGCATGATCCCCGTGATCTTCCTGACAG
AGCGCCAGCGCGCGGCCGGCAAGGACGCCGCCTCGCAGCGGCAGGCCGTGCAGGACGCCGGCAAGGAATCGGCCGCCGTG
CTCGGCTTCTCGGGTGCGATCGGCGCCTACGGCGGCTTCTTCATCCCCAAGAGCTTCGGCACCTCGCTGGACCTGACTGG
TTCGCCCCTCGCCGCGCTCTACTGCTTCATCGTCTTCTACGTGACCTGTGTGCTCGTGACGTGGTGGTTCTACGCCCGCC
GCAACGCCGCAATGCCGTGCTGA

Upstream 100 bases:

>100_bases
GATGCACTACAGCCAGCGCGCGGCAAACGCCAGGCCGACGCCCGAAACCCTGCAAGCCGCCTGAGTTCCGTCTCCTCCCA
AACCAACCTAACTGCATATC

Downstream 100 bases:

>100_bases
CAGAACGAACCCCACAGGAGAACCCTACAAAATGAGTCACTTTCTCGATCGACTGAAGTTCCTGTCGCGCGTGAAGTCCA
CCTACTCGGACGGACACGGC

Product: nitrate/nitrite transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSSSILTRWEPENVAFWRDQGERIAYRNLWISIPALMLAFVIWMLWSVVAVNLDRAGFQFTKNQLFWLTALPALSGATLR
IFYSFLVPIFGGRRFTAISTALLLIPAVGMGFALRDPSTSYPTLLILALLCGLGGANFSSSMANISFFFPKEKKGLATGL
NAGIGNLGVSVVQFVTPLVVSVAVFGALGGEPQTYKAADATQQLWLQNAGFIWVPFIIVASLAAWFGMHDIADARASFAE
QAVIFTRKHNWLMCWLYIGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRPVGGWISDRLGGARVTLWTFI
GMILAVFAVLAALPADGGAGSFPAFLGAFIVLFALTGIGNGSTFRMIPVIFLTERQRAAGKDAASQRQAVQDAGKESAAV
LGFSGAIGAYGGFFIPKSFGTSLDLTGSPLAALYCFIVFYVTCVLVTWWFYARRNAAMPC

Sequences:

>Translated_460_residues
MSSSILTRWEPENVAFWRDQGERIAYRNLWISIPALMLAFVIWMLWSVVAVNLDRAGFQFTKNQLFWLTALPALSGATLR
IFYSFLVPIFGGRRFTAISTALLLIPAVGMGFALRDPSTSYPTLLILALLCGLGGANFSSSMANISFFFPKEKKGLATGL
NAGIGNLGVSVVQFVTPLVVSVAVFGALGGEPQTYKAADATQQLWLQNAGFIWVPFIIVASLAAWFGMHDIADARASFAE
QAVIFTRKHNWLMCWLYIGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRPVGGWISDRLGGARVTLWTFI
GMILAVFAVLAALPADGGAGSFPAFLGAFIVLFALTGIGNGSTFRMIPVIFLTERQRAAGKDAASQRQAVQDAGKESAAV
LGFSGAIGAYGGFFIPKSFGTSLDLTGSPLAALYCFIVFYVTCVLVTWWFYARRNAAMPC
>Mature_459_residues
SSSILTRWEPENVAFWRDQGERIAYRNLWISIPALMLAFVIWMLWSVVAVNLDRAGFQFTKNQLFWLTALPALSGATLRI
FYSFLVPIFGGRRFTAISTALLLIPAVGMGFALRDPSTSYPTLLILALLCGLGGANFSSSMANISFFFPKEKKGLATGLN
AGIGNLGVSVVQFVTPLVVSVAVFGALGGEPQTYKAADATQQLWLQNAGFIWVPFIIVASLAAWFGMHDIADARASFAEQ
AVIFTRKHNWLMCWLYIGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRPVGGWISDRLGGARVTLWTFIG
MILAVFAVLAALPADGGAGSFPAFLGAFIVLFALTGIGNGSTFRMIPVIFLTERQRAAGKDAASQRQAVQDAGKESAAVL
GFSGAIGAYGGFFIPKSFGTSLDLTGSPLAALYCFIVFYVTCVLVTWWFYARRNAAMPC

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=454, Percent_Identity=55.2863436123348, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1787743, Length=457, Percent_Identity=54.7045951859956, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49438; Mature: 49307

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSILTRWEPENVAFWRDQGERIAYRNLWISIPALMLAFVIWMLWSVVAVNLDRAGFQF
CCCCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TKNQLFWLTALPALSGATLRIFYSFLVPIFGGRRFTAISTALLLIPAVGMGFALRDPSTS
CCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCC
YPTLLILALLCGLGGANFSSSMANISFFFPKEKKGLATGLNAGIGNLGVSVVQFVTPLVV
CHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHH
SVAVFGALGGEPQTYKAADATQQLWLQNAGFIWVPFIIVASLAAWFGMHDIADARASFAE
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVIFTRKHNWLMCWLYIGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRP
HHEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
VGGWISDRLGGARVTLWTFIGMILAVFAVLAALPADGGAGSFPAFLGAFIVLFALTGIGN
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
GSTFRMIPVIFLTERQRAAGKDAASQRQAVQDAGKESAAVLGFSGAIGAYGGFFIPKSFG
CCCEEEEEEEEEECHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCCCCCC
TSLDLTGSPLAALYCFIVFYVTCVLVTWWFYARRNAAMPC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSSILTRWEPENVAFWRDQGERIAYRNLWISIPALMLAFVIWMLWSVVAVNLDRAGFQF
CCCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TKNQLFWLTALPALSGATLRIFYSFLVPIFGGRRFTAISTALLLIPAVGMGFALRDPSTS
CCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCC
YPTLLILALLCGLGGANFSSSMANISFFFPKEKKGLATGLNAGIGNLGVSVVQFVTPLVV
CHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHH
SVAVFGALGGEPQTYKAADATQQLWLQNAGFIWVPFIIVASLAAWFGMHDIADARASFAE
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVIFTRKHNWLMCWLYIGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRP
HHEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
VGGWISDRLGGARVTLWTFIGMILAVFAVLAALPADGGAGSFPAFLGAFIVLFALTGIGN
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
GSTFRMIPVIFLTERQRAAGKDAASQRQAVQDAGKESAAVLGFSGAIGAYGGFFIPKSFG
CCCEEEEEEEEEECHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCCCCCC
TSLDLTGSPLAALYCFIVFYVTCVLVTWWFYARRNAAMPC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]