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The map label for this gene is narK [H]

Identifier: 94311010

GI number: 94311010

Start: 2247655

End: 2248938

Strand: Direct

Name: narK [H]

Synonym: Rmet_2072

Alternate gene names: 94311010

Gene position: 2247655-2248938 (Clockwise)

Preceding gene: 94311009

Following gene: 94311011

Centisome position: 57.22

GC content: 64.49

Gene sequence:

>1284_bases
ATGGCCACCCCAAACCTCCCCTCCAGCAAGCCAGAACCCGTCTCTTCCGGTGCTTGGACGACGCTCTGGTCCAGCACCTT
TGCCTTCACGATCTGTTTCGCCGTCTGGATGCTGTTTGCCGTGCTCGGCATTCCGCTCAAGAAGGAACTCGGCCTGAATG
ACACCGAGTTCGGCCTGCTGGCAGCCACGCCGGTGCTGAGCGGCTCCCTGATCCGCGTGCCGCTCGGCATCTGGACGGAC
CGTTACGGCGGCCGCATCGTGTTCTTTGTGCTGATGCTGCTGACGGTGATCCCCATCTGGCTGATCTCGTACGCGCATAC
GCTGTGGCAATTGCTGGTGCTCGGGCTGTTCGTCGGCCTGGCGGGCGGCTCGTTCTCGGTGGGCACGCCATACGTGGCCC
GCTGGTTCCCGCGCTCGCGCCAGGGCCTGGCCATGGGCATCTTCGGTGCCGGCAACTCGGGCGCCGCCTTGACCAAGTTC
GTCGCGCCCGCTCTGATCCTGGCCGCCGGCACCTGGGAAATCGTGCCGAGGGTGTACTCGGTGGCGATGCTGATCACCGC
CATCGTGTTCTGGCTGTTCTCGCGCAGCAATCCCGCGCACCGCGTTTCGTCGGCCACCAGCTGGCGTGCGCAACTTGCCG
TCATGCGTGATCCGCGTGTCTGGCGCTATTCGCAGTACTACTCGGTCGTGTTCGGCGGCTACGTTGGCCTCTCGCTGTGG
ATGACCAAGTACTACATCGGTGAGTACGGCTTCGACATGAAGACGGCCGCGTTCCTCGCTGCCTGCTTCTCGCTGCCGGG
TGGCGTGCTCCGGGCGATCGGCGGCTGGATCTCGGACCGCTACGGCGCGCACCGCACCACGTGGTGGGTCATGTGGGTCA
GCTGGGTCACGTTCTTCCTGCTCAGCTACCCGAGGAGCGACTTCACGATCCACACCGCCAACGGCCCGGAGAGCTTCCAT
ATCGCGCTCTCGCCAACGGTGTTCACCATCCTGATGTTCGTGGTCGGTATCGCGTTCGCGGTCGGCAAGGCCTCGGTCTT
CAAATTCATCTCGAACGATTTCACACACAACATCGGCGCGGTCTCCGGCGTGGTCGGTCTGGCCGGCGGCCTCGGTGGAT
TCATCCTCCCGATCCTGTTCGGCGCACTGGCTGACCTGACCGGCATCCGCACCAGCTGTTTCATGCTGATGTACGGCGCG
GTCTGCGTGAGTCTGGTGTGGATGCACTACAGCCAGCGCGCGGCAAACGCCAGGCCGACGCCCGAAACCCTGCAAGCCGC
CTGA

Upstream 100 bases:

>100_bases
ATAGCCCCTCCCCCGCCTACCTCTACGCCCGGCGAATAGTCGGCCAATCGCCACAGGCCTAATCTGACGTCACTCCCCAA
AGTGACTTCGGAACCCGGCC

Downstream 100 bases:

>100_bases
GTTCCGTCTCCTCCCAAACCAACCTAACTGCATATCATGTCAAGTTCCATACTGACCCGCTGGGAGCCCGAGAACGTGGC
CTTCTGGCGCGACCAGGGCG

Product: major facilitator superfamily transporter

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MATPNLPSSKPEPVSSGAWTTLWSSTFAFTICFAVWMLFAVLGIPLKKELGLNDTEFGLLAATPVLSGSLIRVPLGIWTD
RYGGRIVFFVLMLLTVIPIWLISYAHTLWQLLVLGLFVGLAGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKF
VAPALILAAGTWEIVPRVYSVAMLITAIVFWLFSRSNPAHRVSSATSWRAQLAVMRDPRVWRYSQYYSVVFGGYVGLSLW
MTKYYIGEYGFDMKTAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVTFFLLSYPRSDFTIHTANGPESFH
IALSPTVFTILMFVVGIAFAVGKASVFKFISNDFTHNIGAVSGVVGLAGGLGGFILPILFGALADLTGIRTSCFMLMYGA
VCVSLVWMHYSQRAANARPTPETLQAA

Sequences:

>Translated_427_residues
MATPNLPSSKPEPVSSGAWTTLWSSTFAFTICFAVWMLFAVLGIPLKKELGLNDTEFGLLAATPVLSGSLIRVPLGIWTD
RYGGRIVFFVLMLLTVIPIWLISYAHTLWQLLVLGLFVGLAGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKF
VAPALILAAGTWEIVPRVYSVAMLITAIVFWLFSRSNPAHRVSSATSWRAQLAVMRDPRVWRYSQYYSVVFGGYVGLSLW
MTKYYIGEYGFDMKTAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVTFFLLSYPRSDFTIHTANGPESFH
IALSPTVFTILMFVVGIAFAVGKASVFKFISNDFTHNIGAVSGVVGLAGGLGGFILPILFGALADLTGIRTSCFMLMYGA
VCVSLVWMHYSQRAANARPTPETLQAA
>Mature_426_residues
ATPNLPSSKPEPVSSGAWTTLWSSTFAFTICFAVWMLFAVLGIPLKKELGLNDTEFGLLAATPVLSGSLIRVPLGIWTDR
YGGRIVFFVLMLLTVIPIWLISYAHTLWQLLVLGLFVGLAGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFV
APALILAAGTWEIVPRVYSVAMLITAIVFWLFSRSNPAHRVSSATSWRAQLAVMRDPRVWRYSQYYSVVFGGYVGLSLWM
TKYYIGEYGFDMKTAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVTFFLLSYPRSDFTIHTANGPESFHI
ALSPTVFTILMFVVGIAFAVGKASVFKFISNDFTHNIGAVSGVVGLAGGLGGFILPILFGALADLTGIRTSCFMLMYGAV
CVSLVWMHYSQRAANARPTPETLQAA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=450, Percent_Identity=26.4444444444444, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787475, Length=456, Percent_Identity=25.219298245614, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46642; Mature: 46511

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPNLPSSKPEPVSSGAWTTLWSSTFAFTICFAVWMLFAVLGIPLKKELGLNDTEFGLL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHH
AATPVLSGSLIRVPLGIWTDRYGGRIVFFVLMLLTVIPIWLISYAHTLWQLLVLGLFVGL
EECHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAPALILAAGTWEIVPRVYS
CCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
VAMLITAIVFWLFSRSNPAHRVSSATSWRAQLAVMRDPRVWRYSQYYSVVFGGYVGLSLW
HHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHHHHH
MTKYYIGEYGFDMKTAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVTFFL
HHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LSYPRSDFTIHTANGPESFHIALSPTVFTILMFVVGIAFAVGKASVFKFISNDFTHNIGA
HHCCCCCCEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSGVVGLAGGLGGFILPILFGALADLTGIRTSCFMLMYGAVCVSLVWMHYSQRAANARPT
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PETLQAA
CHHHCCC
>Mature Secondary Structure 
ATPNLPSSKPEPVSSGAWTTLWSSTFAFTICFAVWMLFAVLGIPLKKELGLNDTEFGLL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHH
AATPVLSGSLIRVPLGIWTDRYGGRIVFFVLMLLTVIPIWLISYAHTLWQLLVLGLFVGL
EECHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAPALILAAGTWEIVPRVYS
CCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
VAMLITAIVFWLFSRSNPAHRVSSATSWRAQLAVMRDPRVWRYSQYYSVVFGGYVGLSLW
HHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHHHHH
MTKYYIGEYGFDMKTAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVTFFL
HHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LSYPRSDFTIHTANGPESFHIALSPTVFTILMFVVGIAFAVGKASVFKFISNDFTHNIGA
HHCCCCCCEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSGVVGLAGGLGGFILPILFGALADLTGIRTSCFMLMYGAVCVSLVWMHYSQRAANARPT
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PETLQAA
CHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]