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The map label for this gene is emrB [H]

Identifier: 94310966

GI number: 94310966

Start: 2193553

End: 2195166

Strand: Direct

Name: emrB [H]

Synonym: Rmet_2028

Alternate gene names: 94310966

Gene position: 2193553-2195166 (Clockwise)

Preceding gene: 94310965

Following gene: 94310975

Centisome position: 55.84

GC content: 61.96

Gene sequence:

>1614_bases
ATGGCAGATTCCATCACCACAGTGCCGGATTCCGGCGCGGCTCCGGCCCCAAAGCCGAAGCCAGCGCCAGCCCGGCATGC
CGCGCCCCCTCCGCTGACGGGCGGCAAGCTCGTCATTGGCACCATCGCCCTGTCGCTGGCTACGTTCATGAACGTGCTCG
ACTCGTCGATCGCCAACGTGTCGATCCCGGCCATCTCGGGCGACCTCGGCGTGTCGCCGAACCAGGGCACCTGGGTCATC
ACGTCGTTCGCCGTCGCCAACGCTATTTCAGTGCCGCTGACGGGCTGGCTGACCACGCGTTTCGGTGCCGTGCGATTGTT
CGTGCTGTCGATCTTGCTGTTCGTGCTCTCGTCCTGGCTGTGCGGCGTGGCGCCGAACCTGGAGACGCTGCTGGCCGCGC
GCGTGCTGCAAGGCGCCGTAGCCGGGCCGATGATCCCACTGTCGCAGTCGCTGCTGCTGGCCAGCTATCCGGTTGCCAAG
AGTTCGATGGCGCTGGCGCTATGGGGCATGACCACACTCGTAGCGCCGATCATGGGCCCCCTGCTTGGCGGCTGGATCTC
CGACAACATGACATGGCCGTGGATCTTCTATATCAACGTGCCGATCGGCATCATGACCGCGTATGCCACATGGGCAATCT
ATCGTGATCGCGAAACACCCACGAAGATCCTGCCAATCGACCGCATCGGCCTGGCTCTCCTCGTGATCTGGGTGGGGTCG
ATGCAGCTCATGCTCGACAAGGGCAAGGAACTCGACTGGTTCCATTCCTCGATCATCGTGACGCTGACGGTGGTGGCGGT
AGTCGGATTCCTGTTCTTCCTGATCTGGGAGAACTATGAGAAGCACCCGATCGTCGACATTCACCTGTTCAAGGGACGCA
ACTTCGCCGCAGGCGTGGTGGCAATCTCCGTGGCGTACGGGCTGTTCTTCGGCAACCTGGTGATTCTGCCGCTGTGGCTG
CAGACCATCATCGGCTACACCGCGACGGACGCCGGTCTGGTGATGGCGCCAGTGGGGATCTTCGCGATCATCCTGTCGCC
AATCATCGGTCGCATCCTGCCGAAGGTCGACTCTCGCTGGGTGGCAACCGCTGCGTTTATTACGTTTGGCGTGGTCAGTT
TCATGCGTGCTGGCTTTACCACAACCGTTGATACGCGCGCGCTGATCATCCCTACGCTGATACAGGGCGCCGCAATGGCA
ATGTTCTTCATCCCGCTGACGTCAATCATCCTGTCCGGCCAGCCGGCCGAGAAGATCCCGGCGGCTTCCGGTTTGTCGAA
CTTTGTACGGATCACGTTCGGCGCGATCGGCGCTTCGATCTCGACGACGATCTGGGAGAACCGCTCGGCGCTGCACCATG
CCCAGTTGGTCGAACAGATCAATCCGTATAACCCGACGTACCAGGCACAGCTCGACCACCTGATGTCGATGGGGATGAAC
CGGATGCAGGCGATCGGCCTGATGGAGCGAAATATCTCGCAGCAGGCATCTATGCTCGGCGCCAACGACATCTTCTGGAT
TTCCGGCGTGCTGTTCTTCGTGCTGATCGGCTTCGTCTGGCTGACCAAGCGCGCCAAGGGCGGCGGCGGCGCGGAAGCAG
CCGGAGCGCACTGA

Upstream 100 bases:

>100_bases
AACGGCGGCCGCACGCCGGCACCGGCGGCGCCGGGCGGCGCCAGCGCGGCGCGCGCAGCCGCCAAGCCTGCCAACACCTG
ATTCGCGGCCTGAGAAGTCC

Downstream 100 bases:

>100_bases
CGTCGAACATCGAACAAAAAAGGCATCGGGTTTATCCGATGCCTTTTTTACTTGGGCGTTGCTTGTGCCGCGTCCTAGAG
TTTGACTAGCCGCTCGGGCC

Product: multidrug efflux system protein (MFS family)

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MADSITTVPDSGAAPAPKPKPAPARHAAPPPLTGGKLVIGTIALSLATFMNVLDSSIANVSIPAISGDLGVSPNQGTWVI
TSFAVANAISVPLTGWLTTRFGAVRLFVLSILLFVLSSWLCGVAPNLETLLAARVLQGAVAGPMIPLSQSLLLASYPVAK
SSMALALWGMTTLVAPIMGPLLGGWISDNMTWPWIFYINVPIGIMTAYATWAIYRDRETPTKILPIDRIGLALLVIWVGS
MQLMLDKGKELDWFHSSIIVTLTVVAVVGFLFFLIWENYEKHPIVDIHLFKGRNFAAGVVAISVAYGLFFGNLVILPLWL
QTIIGYTATDAGLVMAPVGIFAIILSPIIGRILPKVDSRWVATAAFITFGVVSFMRAGFTTTVDTRALIIPTLIQGAAMA
MFFIPLTSIILSGQPAEKIPAASGLSNFVRITFGAIGASISTTIWENRSALHHAQLVEQINPYNPTYQAQLDHLMSMGMN
RMQAIGLMERNISQQASMLGANDIFWISGVLFFVLIGFVWLTKRAKGGGGAEAAGAH

Sequences:

>Translated_537_residues
MADSITTVPDSGAAPAPKPKPAPARHAAPPPLTGGKLVIGTIALSLATFMNVLDSSIANVSIPAISGDLGVSPNQGTWVI
TSFAVANAISVPLTGWLTTRFGAVRLFVLSILLFVLSSWLCGVAPNLETLLAARVLQGAVAGPMIPLSQSLLLASYPVAK
SSMALALWGMTTLVAPIMGPLLGGWISDNMTWPWIFYINVPIGIMTAYATWAIYRDRETPTKILPIDRIGLALLVIWVGS
MQLMLDKGKELDWFHSSIIVTLTVVAVVGFLFFLIWENYEKHPIVDIHLFKGRNFAAGVVAISVAYGLFFGNLVILPLWL
QTIIGYTATDAGLVMAPVGIFAIILSPIIGRILPKVDSRWVATAAFITFGVVSFMRAGFTTTVDTRALIIPTLIQGAAMA
MFFIPLTSIILSGQPAEKIPAASGLSNFVRITFGAIGASISTTIWENRSALHHAQLVEQINPYNPTYQAQLDHLMSMGMN
RMQAIGLMERNISQQASMLGANDIFWISGVLFFVLIGFVWLTKRAKGGGGAEAAGAH
>Mature_536_residues
ADSITTVPDSGAAPAPKPKPAPARHAAPPPLTGGKLVIGTIALSLATFMNVLDSSIANVSIPAISGDLGVSPNQGTWVIT
SFAVANAISVPLTGWLTTRFGAVRLFVLSILLFVLSSWLCGVAPNLETLLAARVLQGAVAGPMIPLSQSLLLASYPVAKS
SMALALWGMTTLVAPIMGPLLGGWISDNMTWPWIFYINVPIGIMTAYATWAIYRDRETPTKILPIDRIGLALLVIWVGSM
QLMLDKGKELDWFHSSIIVTLTVVAVVGFLFFLIWENYEKHPIVDIHLFKGRNFAAGVVAISVAYGLFFGNLVILPLWLQ
TIIGYTATDAGLVMAPVGIFAIILSPIIGRILPKVDSRWVATAAFITFGVVSFMRAGFTTTVDTRALIIPTLIQGAAMAM
FFIPLTSIILSGQPAEKIPAASGLSNFVRITFGAIGASISTTIWENRSALHHAQLVEQINPYNPTYQAQLDHLMSMGMNR
MQAIGLMERNISQQASMLGANDIFWISGVLFFVLIGFVWLTKRAKGGGGAEAAGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=494, Percent_Identity=57.085020242915, Blast_Score=533, Evalue=1e-153,
Organism=Escherichia coli, GI1788710, Length=509, Percent_Identity=46.9548133595285, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1790195, Length=424, Percent_Identity=29.2452830188679, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI87081983, Length=398, Percent_Identity=24.3718592964824, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788392, Length=396, Percent_Identity=27.5252525252525, Blast_Score=112, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6322958, Length=498, Percent_Identity=22.0883534136546, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=152, Percent_Identity=28.9473684210526, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57471; Mature: 57339

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSITTVPDSGAAPAPKPKPAPARHAAPPPLTGGKLVIGTIALSLATFMNVLDSSIANV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCE
SIPAISGDLGVSPNQGTWVITSFAVANAISVPLTGWLTTRFGAVRLFVLSILLFVLSSWL
ECCEECCCCCCCCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CGVAPNLETLLAARVLQGAVAGPMIPLSQSLLLASYPVAKSSMALALWGMTTLVAPIMGP
HCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LLGGWISDNMTWPWIFYINVPIGIMTAYATWAIYRDRETPTKILPIDRIGLALLVIWVGS
HHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHEEECCCCCCEECCHHHHHHHHHHHHHCC
MQLMLDKGKELDWFHSSIIVTLTVVAVVGFLFFLIWENYEKHPIVDIHLFKGRNFAAGVV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHH
AISVAYGLFFGNLVILPLWLQTIIGYTATDAGLVMAPVGIFAIILSPIIGRILPKVDSRW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VATAAFITFGVVSFMRAGFTTTVDTRALIIPTLIQGAAMAMFFIPLTSIILSGQPAEKIP
HHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
AASGLSNFVRITFGAIGASISTTIWENRSALHHAQLVEQINPYNPTYQAQLDHLMSMGMN
CCCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHH
RMQAIGLMERNISQQASMLGANDIFWISGVLFFVLIGFVWLTKRAKGGGGAEAAGAH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ADSITTVPDSGAAPAPKPKPAPARHAAPPPLTGGKLVIGTIALSLATFMNVLDSSIANV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCE
SIPAISGDLGVSPNQGTWVITSFAVANAISVPLTGWLTTRFGAVRLFVLSILLFVLSSWL
ECCEECCCCCCCCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CGVAPNLETLLAARVLQGAVAGPMIPLSQSLLLASYPVAKSSMALALWGMTTLVAPIMGP
HCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LLGGWISDNMTWPWIFYINVPIGIMTAYATWAIYRDRETPTKILPIDRIGLALLVIWVGS
HHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHEEECCCCCCEECCHHHHHHHHHHHHHCC
MQLMLDKGKELDWFHSSIIVTLTVVAVVGFLFFLIWENYEKHPIVDIHLFKGRNFAAGVV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHH
AISVAYGLFFGNLVILPLWLQTIIGYTATDAGLVMAPVGIFAIILSPIIGRILPKVDSRW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VATAAFITFGVVSFMRAGFTTTVDTRALIIPTLIQGAAMAMFFIPLTSIILSGQPAEKIP
HHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
AASGLSNFVRITFGAIGASISTTIWENRSALHHAQLVEQINPYNPTYQAQLDHLMSMGMN
CCCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHH
RMQAIGLMERNISQQASMLGANDIFWISGVLFFVLIGFVWLTKRAKGGGGAEAAGAH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]