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The map label for this gene is emrA [H]

Identifier: 94310965

GI number: 94310965

Start: 2192292

End: 2193533

Strand: Direct

Name: emrA [H]

Synonym: Rmet_2027

Alternate gene names: 94310965

Gene position: 2192292-2193533 (Clockwise)

Preceding gene: 94310964

Following gene: 94310966

Centisome position: 55.81

GC content: 66.51

Gene sequence:

>1242_bases
ATGAGCAACAACCAGCAATCGGCCAGCCAGAAACCCGCTCCAACCGGTGACAGCAAGCGCAAGCGCCTGCTCACGACGCT
CACTGCCGCGATCGTGGTGGCCGGCGTCGGCTATGGCCTGTACTGGGGCCTCTACGCCCGGCACTTCGAAAGCACCGATG
ATGCCTATGTGGCCGGCAACGTGGTCCAGGTGACCCCGCTGGTGGGCGGCACTGTGGTGTCGATCGCCGCCGACGACACG
CAACTCGTCACCGCTGGCCAGCCGCTGATCCAGCTCGACCGCGCCGACACCCAGGTAGCCATGGAACAGGCCGAAGCCCA
ACTCGCGCAAGCCGTGCGTGAAGTGCGCACGCTCTATGTGAACAACGCTTCGCTGGCTGCGAACGTGGCATTGCGCGATG
CCGATGTGGCCAAGGCACGCGATGACCTGCGCCGCCGCCAGGCGATTGCCGGCTCGGGCGCGGTCTCGAGCGAGGAAATC
TCGCACGCCCAGACCGCACTGAAAGCAGCCGAGTCGGCGCTGCTTGCCTCGAAGGAGCAACTCGCGTCGAATCAGGTATT
GACCGACCAGACCACCGTGGAAAAGCACCCGAACGTGCAGCGCGCCGCCGCCAATCTGCGTTCGGCCTACCTGTCGTTCG
CCCGCTCCACACTGCCGGCGCCGGTCACCGGCTACGTGGCCAAGCGCTCGGTACAGGTTGGCCAGCGCGTGGCCGCCGGC
ACACCGCTGATGGCCGTGGTACCGCTCGATCAGGTCTGGGTTGATGCCAACTTCAAGGAAGTGCAGATCACCCATATGCG
CATCGGCCAGCCGGTGGAACTGGAAGCTGACGTCTATGGCTCGAAGGTGAAGTACCAGGGCCATGTCGAGGGCTTCTCGG
CCGGTACCGGGGCGGCGTTCTCGCTCCTGCCCGCGCAGAACGCCACCGGCAACTGGATCAAGGTGGTGCAGCGCCTGCCG
GTGCGTATCGCGCTGGACGCCCAACAACTCAAGGAGCATCCGCTGCGTGTCGGCCTGTCGATGAACGTTACCGTTGACGT
GCGCAAGGAAGAAGGCGCCGCGATGGTCACCGCCACCAACGCCCGGCCTCTGCAGACGGACGTCTATGACAAGGTGGCGC
AGGACGCCGACGAACTGATCGCCCGCATCATCTCCGTCAATAACGGCGGCCGCACGCCGGCACCGGCGGCGCCGGGCGGC
GCCAGCGCGGCGCGCGCAGCCGCCAAGCCTGCCAACACCTGA

Upstream 100 bases:

>100_bases
GACGAAAAAGCCGGCGCCGTAGAACAGAACACGGAAAAGGGCACCCGGGGCTGATACGCCGGGCAATGCAAGACACGACG
AAAATACGTACGAGATCGAT

Downstream 100 bases:

>100_bases
TTCGCGGCCTGAGAAGTCCATGGCAGATTCCATCACCACAGTGCCGGATTCCGGCGCGGCTCCGGCCCCAAAGCCGAAGC
CAGCGCCAGCCCGGCATGCC

Product: multidrug efflux system (MFS family)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSNNQQSASQKPAPTGDSKRKRLLTTLTAAIVVAGVGYGLYWGLYARHFESTDDAYVAGNVVQVTPLVGGTVVSIAADDT
QLVTAGQPLIQLDRADTQVAMEQAEAQLAQAVREVRTLYVNNASLAANVALRDADVAKARDDLRRRQAIAGSGAVSSEEI
SHAQTALKAAESALLASKEQLASNQVLTDQTTVEKHPNVQRAAANLRSAYLSFARSTLPAPVTGYVAKRSVQVGQRVAAG
TPLMAVVPLDQVWVDANFKEVQITHMRIGQPVELEADVYGSKVKYQGHVEGFSAGTGAAFSLLPAQNATGNWIKVVQRLP
VRIALDAQQLKEHPLRVGLSMNVTVDVRKEEGAAMVTATNARPLQTDVYDKVAQDADELIARIISVNNGGRTPAPAAPGG
ASAARAAAKPANT

Sequences:

>Translated_413_residues
MSNNQQSASQKPAPTGDSKRKRLLTTLTAAIVVAGVGYGLYWGLYARHFESTDDAYVAGNVVQVTPLVGGTVVSIAADDT
QLVTAGQPLIQLDRADTQVAMEQAEAQLAQAVREVRTLYVNNASLAANVALRDADVAKARDDLRRRQAIAGSGAVSSEEI
SHAQTALKAAESALLASKEQLASNQVLTDQTTVEKHPNVQRAAANLRSAYLSFARSTLPAPVTGYVAKRSVQVGQRVAAG
TPLMAVVPLDQVWVDANFKEVQITHMRIGQPVELEADVYGSKVKYQGHVEGFSAGTGAAFSLLPAQNATGNWIKVVQRLP
VRIALDAQQLKEHPLRVGLSMNVTVDVRKEEGAAMVTATNARPLQTDVYDKVAQDADELIARIISVNNGGRTPAPAAPGG
ASAARAAAKPANT
>Mature_412_residues
SNNQQSASQKPAPTGDSKRKRLLTTLTAAIVVAGVGYGLYWGLYARHFESTDDAYVAGNVVQVTPLVGGTVVSIAADDTQ
LVTAGQPLIQLDRADTQVAMEQAEAQLAQAVREVRTLYVNNASLAANVALRDADVAKARDDLRRRQAIAGSGAVSSEEIS
HAQTALKAAESALLASKEQLASNQVLTDQTTVEKHPNVQRAAANLRSAYLSFARSTLPAPVTGYVAKRSVQVGQRVAAGT
PLMAVVPLDQVWVDANFKEVQITHMRIGQPVELEADVYGSKVKYQGHVEGFSAGTGAAFSLLPAQNATGNWIKVVQRLPV
RIALDAQQLKEHPLRVGLSMNVTVDVRKEEGAAMVTATNARPLQTDVYDKVAQDADELIARIISVNNGGRTPAPAAPGGA
SAARAAAKPANT

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=390, Percent_Identity=43.8461538461538, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1788711, Length=338, Percent_Identity=42.3076923076923, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI87081951, Length=313, Percent_Identity=27.1565495207668, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790519, Length=310, Percent_Identity=30.9677419354839, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789637, Length=322, Percent_Identity=26.0869565217391, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43627; Mature: 43496

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNQQSASQKPAPTGDSKRKRLLTTLTAAIVVAGVGYGLYWGLYARHFESTDDAYVAGN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
VVQVTPLVGGTVVSIAADDTQLVTAGQPLIQLDRADTQVAMEQAEAQLAQAVREVRTLYV
EEEEEECCCCEEEEEECCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEE
NNASLAANVALRDADVAKARDDLRRRQAIAGSGAVSSEEISHAQTALKAAESALLASKEQ
CCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LASNQVLTDQTTVEKHPNVQRAAANLRSAYLSFARSTLPAPVTGYVAKRSVQVGQRVAAG
HHCCCEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
TPLMAVVPLDQVWVDANFKEVQITHMRIGQPVELEADVYGSKVKYQGHVEGFSAGTGAAF
CCEEEEEECHHEEECCCCCEEEEEEEECCCCCEEECCCCCCEEEEECCCCCCCCCCCCEE
SLLPAQNATGNWIKVVQRLPVRIALDAQQLKEHPLRVGLSMNVTVDVRKEEGAAMVTATN
EEECCCCCCCHHHHHHHHCCCEEEECHHHHHCCCEEECEEEEEEEEEEECCCCEEEEECC
ARPLQTDVYDKVAQDADELIARIISVNNGGRTPAPAAPGGASAARAAAKPANT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
SNNQQSASQKPAPTGDSKRKRLLTTLTAAIVVAGVGYGLYWGLYARHFESTDDAYVAGN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
VVQVTPLVGGTVVSIAADDTQLVTAGQPLIQLDRADTQVAMEQAEAQLAQAVREVRTLYV
EEEEEECCCCEEEEEECCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEE
NNASLAANVALRDADVAKARDDLRRRQAIAGSGAVSSEEISHAQTALKAAESALLASKEQ
CCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LASNQVLTDQTTVEKHPNVQRAAANLRSAYLSFARSTLPAPVTGYVAKRSVQVGQRVAAG
HHCCCEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
TPLMAVVPLDQVWVDANFKEVQITHMRIGQPVELEADVYGSKVKYQGHVEGFSAGTGAAF
CCEEEEEECHHEEECCCCCEEEEEEEECCCCCEEECCCCCCEEEEECCCCCCCCCCCCEE
SLLPAQNATGNWIKVVQRLPVRIALDAQQLKEHPLRVGLSMNVTVDVRKEEGAAMVTATN
EEECCCCCCCHHHHHHHHCCCEEEECHHHHHCCCEEECEEEEEEEEEEECCCCEEEEECC
ARPLQTDVYDKVAQDADELIARIISVNNGGRTPAPAAPGGASAARAAAKPANT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]