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The map label for this gene is lexA

Identifier: 94310919

GI number: 94310919

Start: 2155258

End: 2155914

Strand: Direct

Name: lexA

Synonym: Rmet_1981

Alternate gene names: 94310919

Gene position: 2155258-2155914 (Clockwise)

Preceding gene: 94310910

Following gene: 94310920

Centisome position: 54.87

GC content: 61.34

Gene sequence:

>657_bases
ATGGCGACCCTGACACCCCGGCAGCAGCAGATTTTCGACCTGATCCGCGACACGATCCGCAACACGGGCTTTCCGCCCAC
TCGTGCCGAGATCGCCGCCGAATTCGGCTTCTCCTCCCCCAATTCGGCCGAGGAACACCTGCGGGCTCTGGCGCGCAAGG
GTGTGATCGAGTTGACACCGGGAGCGTCGCGAGGCATTCGCCTGAAGGTGACGCGCAGCGACTCTGAACGACCCGATCAG
TTTTCGTTACCGATGCCGGGCGTGCTGCAATTGACATTGCCATTAGTCGGTCGCGTCGCAGCTGGCAGCCCGATCCTGGC
TGCCGAGCATATCGACCGGCAGTATCAAGTCGATGCCTCTGTCTTTGACGAGCGCCCCGACTATCTACTCCGCGTTCGCG
GCCTGAGCATGCGCGACGCGGGCATTCTGGATGGCGACCTGCTTGCCGTGAAGAAAGCCAGCGAAGCCGCGAACGGCAAG
GTTGTCGTCGCCCGCCTGGGCGATGACGTCACCGTCAAACGCTTGAAGAAACGCGGCGACACGATCGAGTTGATCGCCGA
AAACCCTGACTTCCAGAACATCGTCCTGCATGCAGGACGTGACGAGTTCTCCCTGGAGGGTATCGCAGTTGGCCTGATCA
GATCTTCAGGCTTCTGA

Upstream 100 bases:

>100_bases
GAGCCGGCTGCGCAAAATGTGCTTGCACTTCACCCGATACTGTATAAAATCACAGCATACTGTTTAAACATACAGTGCTT
CGGCACAAGACCTGCGACCC

Downstream 100 bases:

>100_bases
CTCTCCGGACCCAGGCAGCTGGCCACCTGCCCCGGTCACCCGCTTTTGCTTTGCCAACGCCGCCCCGTCCTTTACGGACG
ACGGGTGCGGACTTCTGCTG

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MATLTPRQQQIFDLIRDTIRNTGFPPTRAEIAAEFGFSSPNSAEEHLRALARKGVIELTPGASRGIRLKVTRSDSERPDQ
FSLPMPGVLQLTLPLVGRVAAGSPILAAEHIDRQYQVDASVFDERPDYLLRVRGLSMRDAGILDGDLLAVKKASEAANGK
VVVARLGDDVTVKRLKKRGDTIELIAENPDFQNIVLHAGRDEFSLEGIAVGLIRSSGF

Sequences:

>Translated_218_residues
MATLTPRQQQIFDLIRDTIRNTGFPPTRAEIAAEFGFSSPNSAEEHLRALARKGVIELTPGASRGIRLKVTRSDSERPDQ
FSLPMPGVLQLTLPLVGRVAAGSPILAAEHIDRQYQVDASVFDERPDYLLRVRGLSMRDAGILDGDLLAVKKASEAANGK
VVVARLGDDVTVKRLKKRGDTIELIAENPDFQNIVLHAGRDEFSLEGIAVGLIRSSGF
>Mature_217_residues
ATLTPRQQQIFDLIRDTIRNTGFPPTRAEIAAEFGFSSPNSAEEHLRALARKGVIELTPGASRGIRLKVTRSDSERPDQF
SLPMPGVLQLTLPLVGRVAAGSPILAAEHIDRQYQVDASVFDERPDYLLRVRGLSMRDAGILDGDLLAVKKASEAANGKV
VVARLGDDVTVKRLKKRGDTIELIAENPDFQNIVLHAGRDEFSLEGIAVGLIRSSGF

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=218, Percent_Identity=55.9633027522936, Blast_Score=240, Evalue=6e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_CUPNE (Q9AGM5)

Other databases:

- EMBL:   AF330820
- ProteinModelPortal:   Q9AGM5
- MEROPS:   S24.001
- BRENDA:   3.4.21.88
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 23767; Mature: 23636

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: ACT_SITE 136-136 ACT_SITE 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLTPRQQQIFDLIRDTIRNTGFPPTRAEIAAEFGFSSPNSAEEHLRALARKGVIELTP
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEECC
GASRGIRLKVTRSDSERPDQFSLPMPGVLQLTLPLVGRVAAGSPILAAEHIDRQYQVDAS
CCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHCCCCEEECHH
VFDERPDYLLRVRGLSMRDAGILDGDLLAVKKASEAANGKVVVARLGDDVTVKRLKKRGD
HHCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHCCC
TIELIAENPDFQNIVLHAGRDEFSLEGIAVGLIRSSGF
EEEEEECCCCCCEEEEECCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
ATLTPRQQQIFDLIRDTIRNTGFPPTRAEIAAEFGFSSPNSAEEHLRALARKGVIELTP
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEECC
GASRGIRLKVTRSDSERPDQFSLPMPGVLQLTLPLVGRVAAGSPILAAEHIDRQYQVDAS
CCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHCCCCEEECHH
VFDERPDYLLRVRGLSMRDAGILDGDLLAVKKASEAANGKVVVARLGDDVTVKRLKKRGD
HHCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHCCC
TIELIAENPDFQNIVLHAGRDEFSLEGIAVGLIRSSGF
EEEEEECCCCCCEEEEECCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA