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The map label for this gene is gatA [H]

Identifier: 94310875

GI number: 94310875

Start: 2106043

End: 2107233

Strand: Direct

Name: gatA [H]

Synonym: Rmet_1937

Alternate gene names: 94310875

Gene position: 2106043-2107233 (Clockwise)

Preceding gene: 94310874

Following gene: 94310876

Centisome position: 53.61

GC content: 66.16

Gene sequence:

>1191_bases
ATGAGCCTGGCCACCCATCCCTCGCGCGCCTTTCTGCCCTACCCCGATGCCCCCGTGCCAAACGCGGCCAATGGCCCGCT
TCGCGGCCTGACGTTCGCCGTCAAGGACCTTTTCGACGTCTCCGGCTACCCCACGGGCGGCGGCAATCCGCATGTGTTGG
CACGCTCGGGCATCAAGACCACCACAGCGCCCACCGTCCAGAAACTGCTCGATGCTGGCGCGGCGTTCGTCGGCAAGGTC
CATACGGACGAGCTCGCGTTTTCGATGAACGGGCAGAACCATCACTACGGCGGCCCTTACAACGGCGCGGCGCCCGATCG
CATTACGGGGGGCTCGTCGTCGGGCTCGGCGTCCGCAGTGTCGAACAAGCTGTGCGACTTCGCGCTCGGCACCGACACCG
GTGGCTCGGTCCGCGCACCGGCCAGTCATTGCGGTCTGTTCGGCATACGTCCGTCACATGGCCGGATATCGCTGACCCAC
TGCATGCCACTGTGCGAAACACTGGATACGTGCGGCTTCTTCGCCCGGGATATCGCCACCTTCGCGCGCGTGGCCGATGT
CCTGCTGGGTGCGGATGCCAATCCGCTGCCCAGCCGGCCCAGGCTGTTGCTGGCAGCCGACCTGTTCGAACTGCCAACGC
CCGAGGCGCGCAAGGCGCTTGCACCCGCCACCGCTCGCATCGAGGCCGTGCTCGGTAAAGCCTCGCCCGTTGACGTGGCC
GATCGTCCGCTGTCGGATCTGTACTGGGCCTTTCGTTACGTGCAGGGCTGGGAGGCGTGGCGGGCGGATGGCGCGCTGAT
CGAGAACTATGGACTGCAGCTGGGGCCCGATGTCGCCGGCCGATTCGCGTTCAGCAAGGAGGTAACGGCAGCGCAGTTCG
AGAAATCGAGCGCCGTGCGCCGGGAGTTCACGGCTCACCTGGGCTGTCTGCTGGGTCAAGACGGCATCCTGTTGCTACCG
ACGATGCCGGACATCGCGCCACTGCGTACGACGCCGATGGAAGCACTCGAAGACTACCGGACCACCGCGGCGCATACGCT
ATGCCTGACCCCGCTGACCGGATTCCCTCAACTCAGCCTGCCGCTATCCGGGCGCGACGGCGCGCCACTGGGAGTATCCC
TGATTGGCCCCATGGGGAGCGACCGCAGCCTGATTGCCGTGGCCGAAACATTGATGACGGCGATTGCCTGA

Upstream 100 bases:

>100_bases
CGGCAATGAGCATCGCGTCAGCTGTGTGCGGGTTACCGCGCACGCTGCCAGCGAATGCGTCGCCTGAAACCGTTTGATCG
ATTCCAAGGAAATCGCACCC

Downstream 100 bases:

>100_bases
ACTAAGCTGGTGCCAGACCGCTCCCCGATCCTCGTCGGCGGCGCGGATGGCGCCAACCGCTCCCATCACGGCAATCACAT
CATGACCCGACTCCGCATCT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSLATHPSRAFLPYPDAPVPNAANGPLRGLTFAVKDLFDVSGYPTGGGNPHVLARSGIKTTTAPTVQKLLDAGAAFVGKV
HTDELAFSMNGQNHHYGGPYNGAAPDRITGGSSSGSASAVSNKLCDFALGTDTGGSVRAPASHCGLFGIRPSHGRISLTH
CMPLCETLDTCGFFARDIATFARVADVLLGADANPLPSRPRLLLAADLFELPTPEARKALAPATARIEAVLGKASPVDVA
DRPLSDLYWAFRYVQGWEAWRADGALIENYGLQLGPDVAGRFAFSKEVTAAQFEKSSAVRREFTAHLGCLLGQDGILLLP
TMPDIAPLRTTPMEALEDYRTTAAHTLCLTPLTGFPQLSLPLSGRDGAPLGVSLIGPMGSDRSLIAVAETLMTAIA

Sequences:

>Translated_396_residues
MSLATHPSRAFLPYPDAPVPNAANGPLRGLTFAVKDLFDVSGYPTGGGNPHVLARSGIKTTTAPTVQKLLDAGAAFVGKV
HTDELAFSMNGQNHHYGGPYNGAAPDRITGGSSSGSASAVSNKLCDFALGTDTGGSVRAPASHCGLFGIRPSHGRISLTH
CMPLCETLDTCGFFARDIATFARVADVLLGADANPLPSRPRLLLAADLFELPTPEARKALAPATARIEAVLGKASPVDVA
DRPLSDLYWAFRYVQGWEAWRADGALIENYGLQLGPDVAGRFAFSKEVTAAQFEKSSAVRREFTAHLGCLLGQDGILLLP
TMPDIAPLRTTPMEALEDYRTTAAHTLCLTPLTGFPQLSLPLSGRDGAPLGVSLIGPMGSDRSLIAVAETLMTAIA
>Mature_395_residues
SLATHPSRAFLPYPDAPVPNAANGPLRGLTFAVKDLFDVSGYPTGGGNPHVLARSGIKTTTAPTVQKLLDAGAAFVGKVH
TDELAFSMNGQNHHYGGPYNGAAPDRITGGSSSGSASAVSNKLCDFALGTDTGGSVRAPASHCGLFGIRPSHGRISLTHC
MPLCETLDTCGFFARDIATFARVADVLLGADANPLPSRPRLLLAADLFELPTPEARKALAPATARIEAVLGKASPVDVAD
RPLSDLYWAFRYVQGWEAWRADGALIENYGLQLGPDVAGRFAFSKEVTAAQFEKSSAVRREFTAHLGCLLGQDGILLLPT
MPDIAPLRTTPMEALEDYRTTAAHTLCLTPLTGFPQLSLPLSGRDGAPLGVSLIGPMGSDRSLIAVAETLMTAIA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=441, Percent_Identity=26.530612244898, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI195972892, Length=412, Percent_Identity=24.2718446601942, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=433, Percent_Identity=27.2517321016166, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=181, Percent_Identity=29.8342541436464, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=385, Percent_Identity=26.4935064935065, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=431, Percent_Identity=26.2180974477958, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356731, Length=400, Percent_Identity=26.25, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648113, Length=172, Percent_Identity=36.046511627907, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 41531; Mature: 41400

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLATHPSRAFLPYPDAPVPNAANGPLRGLTFAVKDLFDVSGYPTGGGNPHVLARSGIKT
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCC
TTAPTVQKLLDAGAAFVGKVHTDELAFSMNGQNHHYGGPYNGAAPDRITGGSSSGSASAV
CCHHHHHHHHHCCHHHHCCEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
SNKLCDFALGTDTGGSVRAPASHCGLFGIRPSHGRISLTHCMPLCETLDTCGFFARDIAT
HHHHHHEEECCCCCCCCCCCHHHCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
FARVADVLLGADANPLPSRPRLLLAADLFELPTPEARKALAPATARIEAVLGKASPVDVA
HHHHHHHHHCCCCCCCCCCCCEEEEEHHHHCCCCHHHHHHCCHHHHHHHHHCCCCCCCCC
DRPLSDLYWAFRYVQGWEAWRADGALIENYGLQLGPDVAGRFAFSKEVTAAQFEKSSAVR
CCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEECCCHHHHHHHHHCHHHHHHHHHHHHH
REFTAHLGCLLGQDGILLLPTMPDIAPLRTTPMEALEDYRTTAAHTLCLTPLTGFPQLSL
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCEEC
PLSGRDGAPLGVSLIGPMGSDRSLIAVAETLMTAIA
CCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLATHPSRAFLPYPDAPVPNAANGPLRGLTFAVKDLFDVSGYPTGGGNPHVLARSGIKT
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCC
TTAPTVQKLLDAGAAFVGKVHTDELAFSMNGQNHHYGGPYNGAAPDRITGGSSSGSASAV
CCHHHHHHHHHCCHHHHCCEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
SNKLCDFALGTDTGGSVRAPASHCGLFGIRPSHGRISLTHCMPLCETLDTCGFFARDIAT
HHHHHHEEECCCCCCCCCCCHHHCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
FARVADVLLGADANPLPSRPRLLLAADLFELPTPEARKALAPATARIEAVLGKASPVDVA
HHHHHHHHHCCCCCCCCCCCCEEEEEHHHHCCCCHHHHHHCCHHHHHHHHHCCCCCCCCC
DRPLSDLYWAFRYVQGWEAWRADGALIENYGLQLGPDVAGRFAFSKEVTAAQFEKSSAVR
CCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEECCCHHHHHHHHHCHHHHHHHHHHHHH
REFTAHLGCLLGQDGILLLPTMPDIAPLRTTPMEALEDYRTTAAHTLCLTPLTGFPQLSL
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCEEC
PLSGRDGAPLGVSLIGPMGSDRSLIAVAETLMTAIA
CCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA