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The map label for this gene is trmC

Identifier: 94310825

GI number: 94310825

Start: 2053319

End: 2055337

Strand: Reverse

Name: trmC

Synonym: Rmet_1887

Alternate gene names: 94310825

Gene position: 2055337-2053319 (Counterclockwise)

Preceding gene: 94310827

Following gene: 94310824

Centisome position: 52.32

GC content: 65.97

Gene sequence:

>2019_bases
ATGACGCGCGTTCTTGAGCCCGCCGAACCCATTCTGTCCAATGACGGTACTCCCTATTCGCCTCGCTATGACGATGTCTA
TCACAGCGCGCGTGGAGGTCTGGCGCAGGCACATCACGTGTTTCTCGGTGGCAATGGTCTGCCCGAAGGCTGGGCCGGGC
GCGAGCAGTTTGTCATTGTCGAGACGGGGTTTGGGCAGGGCCTGAATTTCCTCGCCACCTGGCAGGCATGGCGTAGCGAT
CCGCAGCGTTGCCGACGGCTGCATTTCGTGTCGATCGAGAAACATCCCTTCACGCGCGAAGGTCTGGCGCGATTACATGC
ACATGCTGGCCTGGGTGATCTGCTGCCGTTATCCGACCAATTGCAGCAACAATGGCCTGATGCGTTGCCCGGCTTGCACA
GACTGTCGTTCGAGGATGGGGCGGTCACGCTGACGCTGGCGTTGGGCGACGTGGAAACGATGTTGCCGAAGCTAGTGCTT
GGCGCCGATGCGTTCTACCTCGATGGGTTTTCGCCATCGCGCAACGCCGAGATGTGGTCCGACACCGTGTTCCGCGGCCT
GGCGCGTCTCGCCCGCATCGGTGCGACGCTCGCGACCTACACAGCGGCGGGATTCGTGAGGCGCGGGCTCACGGCGGCCG
GGTTCGAGGCGCACAAGGCGCCCGGATTTGGTGGCAAGCGAGACATGACCGTGGCGCGTTTCGCGCCAGTTTGGAAGAAC
CGCCGTCATGCGCCGCCCGAGGCAGCGCAATGGGCCGAGCGACATGCCATCGTCATTGGAGCGGGGCTGGCGGGATGCGC
CGTGACCGAACGTCTGGCTGCGCGTGGCTGGCGCGTGACATTGCTCGACGCGCATGACGGACCGGCGCGACAGACATCGT
GCCACCGTGCCGCGGCGATGCATGCGCATGTGTCCGCCGACGACAGCTTCCTTTCGCGACTGTCCCGTGCTGGCAACCTT
CATGCGTTGCGCGCGTGGGGCGCATTGGAGGCGGCTGGCTATCCCGTGGGATGGCACGGCACTGGCGTCCTGCAGATCGG
CGAGGATGATGCTGAGAACGCGGCGCAGCAAGCCGCACTTGCGGAGCTTCGGTTTCCGGAGTCATTTGTGCGCTGGATGT
CGCCGCAAGAGGCTACGGAGCAGCATGGCGCAACGGTTCCGAGAGGCGGCTTGTGGTTTCCGAAGGGAGGCTGGGTGGCG
CCGCCCGACATTTGCCGCGCGCAGCTTGCGAAAGCTGGTGCGGCTGTGGATGCGCTATTCAATTGCCGCGTGGCAGCGAT
CAGGCGGATCGGTGATGCGTGGCAGGCGCTTGCCGATGACGGTGGGGTATTGGCCGCAGCGCCGGTTCTGGTGCTCGCCA
ATGCCTACGAAGCCGATCGGCTGACCTCGGGCCAGTTCCTGGCGCTGCGCCGGGTGCGTGGCCAACTGACGGATCTGGCA
CCCGCGCAGGCAGAGGCGCTGGGTGGTTGGCCCGATTGCGTCGTGACCGGCGGTGGTTACCTACTGCCGCGTGACGCGGC
GGGCGGAGCAAGGATGGGCTCGAGTTATGAAGCGGACGAAGGGCCGCTGGTCGAGCGTCCGGAAGTGCACGCTGCCAACC
TCGATCGTCTGGCATCGATGCTGCCGGACCGGGCGTCACAAATCGCGCAGATCGACCCCGCCACGCTGCAGGGTTACGTC
GGCGTGCGCACGGTCACGTATAACCGTCTTCCGCTTGTCGGGCGCATTGCCGACGATGCGCAGGCACTGTCCCGTGCCGC
TGCGTTGCGTGGCGCGCATTTACGCGATCTGCCGCGGCTTCCCGGTCTGTATGCGGCCCTTGCATATGCCTCGCGGGGCC
TCACATGGGCAGCCTTGTGTGCGGAGCTGATCGCCAGCCAGATCGAGGGCGAACCACTGCCGCTCGAGGCCGATCTGGCA
GATGCGATCGATCCCGCACGGCTCCTGCTGCGCGCGCTCCGACACGGTCACGCGCAGGCGCCCGAATCGGCGGGGCCTGG
GGTGGATGCGGCCGGCTAG

Upstream 100 bases:

>100_bases
ACTTCGCGCAAATACGGCCTTGAAGGCATTTTCAGGGCGGTGCGCGGGGAGGGCGCTATGCTACCATCCGCCGCCATTTC
CGGCCGTATGAATCCTTGCC

Downstream 100 bases:

>100_bases
GGTAAAAACTGCCGGAAGCGAATCAAAACCGTGCGATAATCCCGGTTTTCCGTTTGCACGGAGACCCCCGCTCGTCGGCG
CCAGTCGCGGGTGGACCGAT

Product: bifunctional tRNA (mnm(5)s(2)U34)-methyltransferase/FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MTRVLEPAEPILSNDGTPYSPRYDDVYHSARGGLAQAHHVFLGGNGLPEGWAGREQFVIVETGFGQGLNFLATWQAWRSD
PQRCRRLHFVSIEKHPFTREGLARLHAHAGLGDLLPLSDQLQQQWPDALPGLHRLSFEDGAVTLTLALGDVETMLPKLVL
GADAFYLDGFSPSRNAEMWSDTVFRGLARLARIGATLATYTAAGFVRRGLTAAGFEAHKAPGFGGKRDMTVARFAPVWKN
RRHAPPEAAQWAERHAIVIGAGLAGCAVTERLAARGWRVTLLDAHDGPARQTSCHRAAAMHAHVSADDSFLSRLSRAGNL
HALRAWGALEAAGYPVGWHGTGVLQIGEDDAENAAQQAALAELRFPESFVRWMSPQEATEQHGATVPRGGLWFPKGGWVA
PPDICRAQLAKAGAAVDALFNCRVAAIRRIGDAWQALADDGGVLAAAPVLVLANAYEADRLTSGQFLALRRVRGQLTDLA
PAQAEALGGWPDCVVTGGGYLLPRDAAGGARMGSSYEADEGPLVERPEVHAANLDRLASMLPDRASQIAQIDPATLQGYV
GVRTVTYNRLPLVGRIADDAQALSRAAALRGAHLRDLPRLPGLYAALAYASRGLTWAALCAELIASQIEGEPLPLEADLA
DAIDPARLLLRALRHGHAQAPESAGPGVDAAG

Sequences:

>Translated_672_residues
MTRVLEPAEPILSNDGTPYSPRYDDVYHSARGGLAQAHHVFLGGNGLPEGWAGREQFVIVETGFGQGLNFLATWQAWRSD
PQRCRRLHFVSIEKHPFTREGLARLHAHAGLGDLLPLSDQLQQQWPDALPGLHRLSFEDGAVTLTLALGDVETMLPKLVL
GADAFYLDGFSPSRNAEMWSDTVFRGLARLARIGATLATYTAAGFVRRGLTAAGFEAHKAPGFGGKRDMTVARFAPVWKN
RRHAPPEAAQWAERHAIVIGAGLAGCAVTERLAARGWRVTLLDAHDGPARQTSCHRAAAMHAHVSADDSFLSRLSRAGNL
HALRAWGALEAAGYPVGWHGTGVLQIGEDDAENAAQQAALAELRFPESFVRWMSPQEATEQHGATVPRGGLWFPKGGWVA
PPDICRAQLAKAGAAVDALFNCRVAAIRRIGDAWQALADDGGVLAAAPVLVLANAYEADRLTSGQFLALRRVRGQLTDLA
PAQAEALGGWPDCVVTGGGYLLPRDAAGGARMGSSYEADEGPLVERPEVHAANLDRLASMLPDRASQIAQIDPATLQGYV
GVRTVTYNRLPLVGRIADDAQALSRAAALRGAHLRDLPRLPGLYAALAYASRGLTWAALCAELIASQIEGEPLPLEADLA
DAIDPARLLLRALRHGHAQAPESAGPGVDAAG
>Mature_671_residues
TRVLEPAEPILSNDGTPYSPRYDDVYHSARGGLAQAHHVFLGGNGLPEGWAGREQFVIVETGFGQGLNFLATWQAWRSDP
QRCRRLHFVSIEKHPFTREGLARLHAHAGLGDLLPLSDQLQQQWPDALPGLHRLSFEDGAVTLTLALGDVETMLPKLVLG
ADAFYLDGFSPSRNAEMWSDTVFRGLARLARIGATLATYTAAGFVRRGLTAAGFEAHKAPGFGGKRDMTVARFAPVWKNR
RHAPPEAAQWAERHAIVIGAGLAGCAVTERLAARGWRVTLLDAHDGPARQTSCHRAAAMHAHVSADDSFLSRLSRAGNLH
ALRAWGALEAAGYPVGWHGTGVLQIGEDDAENAAQQAALAELRFPESFVRWMSPQEATEQHGATVPRGGLWFPKGGWVAP
PDICRAQLAKAGAAVDALFNCRVAAIRRIGDAWQALADDGGVLAAAPVLVLANAYEADRLTSGQFLALRRVRGQLTDLAP
AQAEALGGWPDCVVTGGGYLLPRDAAGGARMGSSYEADEGPLVERPEVHAANLDRLASMLPDRASQIAQIDPATLQGYVG
VRTVTYNRLPLVGRIADDAQALSRAAALRGAHLRDLPRLPGLYAALAYASRGLTWAALCAELIASQIEGEPLPLEADLAD
AIDPARLLLRALRHGHAQAPESAGPGVDAAG

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=681, Percent_Identity=34.3612334801762, Blast_Score=330, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_RALME (Q1LM60)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584035.1
- ProteinModelPortal:   Q1LM60
- SMR:   Q1LM60
- STRING:   Q1LM60
- GeneID:   4038691
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1887
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- PhylomeDB:   Q1LM60
- ProtClustDB:   PRK01747
- BioCyc:   RMET266264:RMET_1887-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 71706; Mature: 71575

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVLEPAEPILSNDGTPYSPRYDDVYHSARGGLAQAHHVFLGGNGLPEGWAGREQFVIV
CCCCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEE
ETGFGQGLNFLATWQAWRSDPQRCRRLHFVSIEKHPFTREGLARLHAHAGLGDLLPLSDQ
ECCCCCCCHHHHHHHHHCCCHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHH
LQQQWPDALPGLHRLSFEDGAVTLTLALGDVETMLPKLVLGADAFYLDGFSPSRNAEMWS
HHHHCHHHCCCCEEEECCCCCEEEEEEECCHHHHHHHHHHCCCEEEECCCCCCCCCHHHH
DTVFRGLARLARIGATLATYTAAGFVRRGLTAAGFEAHKAPGFGGKRDMTVARFAPVWKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHC
RRHAPPEAAQWAERHAIVIGAGLAGCAVTERLAARGWRVTLLDAHDGPARQTSCHRAAAM
CCCCCCHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
HAHVSADDSFLSRLSRAGNLHALRAWGALEAAGYPVGWHGTGVLQIGEDDAENAAQQAAL
HHCCCCCHHHHHHHHHCCCEEEEHHCCCHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHH
AELRFPESFVRWMSPQEATEQHGATVPRGGLWFPKGGWVAPPDICRAQLAKAGAAVDALF
HHHCCCHHHHHHCCCHHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
NCRVAAIRRIGDAWQALADDGGVLAAAPVLVLANAYEADRLTSGQFLALRRVRGQLTDLA
HHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCC
PAQAEALGGWPDCVVTGGGYLLPRDAAGGARMGSSYEADEGPLVERPEVHAANLDRLASM
CHHHHHCCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LPDRASQIAQIDPATLQGYVGVRTVTYNRLPLVGRIADDAQALSRAAALRGAHLRDLPRL
CCHHHHHHHHCCCHHHHCCEEEEEEEECCCCEEEECHHHHHHHHHHHHHCCCHHHHCCCC
PGLYAALAYASRGLTWAALCAELIASQIEGEPLPLEADLADAIDPARLLLRALRHGHAQA
CHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCC
PESAGPGVDAAG
CCCCCCCCCCCC
>Mature Secondary Structure 
TRVLEPAEPILSNDGTPYSPRYDDVYHSARGGLAQAHHVFLGGNGLPEGWAGREQFVIV
CCCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEE
ETGFGQGLNFLATWQAWRSDPQRCRRLHFVSIEKHPFTREGLARLHAHAGLGDLLPLSDQ
ECCCCCCCHHHHHHHHHCCCHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHH
LQQQWPDALPGLHRLSFEDGAVTLTLALGDVETMLPKLVLGADAFYLDGFSPSRNAEMWS
HHHHCHHHCCCCEEEECCCCCEEEEEEECCHHHHHHHHHHCCCEEEECCCCCCCCCHHHH
DTVFRGLARLARIGATLATYTAAGFVRRGLTAAGFEAHKAPGFGGKRDMTVARFAPVWKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHC
RRHAPPEAAQWAERHAIVIGAGLAGCAVTERLAARGWRVTLLDAHDGPARQTSCHRAAAM
CCCCCCHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
HAHVSADDSFLSRLSRAGNLHALRAWGALEAAGYPVGWHGTGVLQIGEDDAENAAQQAAL
HHCCCCCHHHHHHHHHCCCEEEEHHCCCHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHH
AELRFPESFVRWMSPQEATEQHGATVPRGGLWFPKGGWVAPPDICRAQLAKAGAAVDALF
HHHCCCHHHHHHCCCHHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
NCRVAAIRRIGDAWQALADDGGVLAAAPVLVLANAYEADRLTSGQFLALRRVRGQLTDLA
HHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCC
PAQAEALGGWPDCVVTGGGYLLPRDAAGGARMGSSYEADEGPLVERPEVHAANLDRLASM
CHHHHHCCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LPDRASQIAQIDPATLQGYVGVRTVTYNRLPLVGRIADDAQALSRAAALRGAHLRDLPRL
CCHHHHHHHHCCCHHHHCCEEEEEEEECCCCEEEECHHHHHHHHHHHHHCCCHHHHCCCC
PGLYAALAYASRGLTWAALCAELIASQIEGEPLPLEADLADAIDPARLLLRALRHGHAQA
CHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCC
PESAGPGVDAAG
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA