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The map label for this gene is gadB [H]

Identifier: 94310704

GI number: 94310704

Start: 1917382

End: 1918764

Strand: Direct

Name: gadB [H]

Synonym: Rmet_1766

Alternate gene names: 94310704

Gene position: 1917382-1918764 (Clockwise)

Preceding gene: 94310702

Following gene: 94310706

Centisome position: 48.81

GC content: 60.95

Gene sequence:

>1383_bases
ATGAGCGACATGCAAGAGCACAAGGTCACACCGTACGACCAGTTCAACTACGAGGTGCCTGAAAACGTCTTCCCGCGCGA
AGGCATCTCGGCGAATGCAGCGGCCGCGATCGTGATCAGTGACGAATGGACCGACACCAACCCCATGCTCAACATGTCGT
CGTTCGTCACCACGTTCGCGGAACCGGAAGCCCGGGAAGTCGCCGCGCGCAATATCTTCAAGAACTATATCGACCACGAC
ATGTATCCGCGGCTCTTCGCCATGGAAGGCCGCATGGTGCGCTGGCTGCATGAACTGTGGAACGGTCCGAAGGGCGTGGA
GCCGTACGGCACGTGTACGGTAGGCTCATCCGAAGCCTGCATGCTCGGGGGCCTGGCGCACAAGTGGAACTGGCGCCAGC
GCCGCCAGGCCGAAGGCAAGGATGCCACCCGCCCGAACATGGTGACGGGCGGCAACGTGCAGATCGTGTGGAAGAAGTTT
CTGCGCTACTTCGACGTCGAGCCGCGCATCGTGCCGCTGAAGCCGGGCAACTACTGCCTGACCGCCGAAGACCTGGACAA
GTACGTCGATGAGAACACGATCTGCGTCGTGGCCATCGCCGGGCAGACCTTCACGGGCGAGGATGACGACATCCAGGGCA
TTCACGACTGGCTCGATGCCTACGAGAAGCGCACGGGCATCTCGATCCCCATGCATATCGACGGCGCCTCCGGCGGCTTC
GTCAACCCGTTCCTCTACCCCGACTACAAGTGGGACTTCCGCCTGCCGCGCGTGCAGTCGATCAACGCCTCGGGCCACAA
GTACGGGCTGACGCCGCCGGGCCTCGGCTGGGTGGTGTTCCGCGAACGCAAGATCTTCAACGAGGAACTGGTGTTCTACG
TGAACTACTTGGGCGGAGAAATGCCCACGGCCACGCTCAACTTCAGCCGCAACGCATTCCAGGTGGCCGTGCAGTACTAC
CAGTTCCTGCGGCTCGGGTTCGACGGCTACAAACGCGTGATGCAGCACACGCTGGACAACGCGATCTTCCTGCGCCAGCA
GCTCGTCGACAGCGGGTACTTCGACATCATGAACACCACGCAGCGCATTCCGGTGGTGGCAGTGACGCTGAACAAGAAGT
ACAAGAACTTCAACGAGTTCGATGTCTCCAACAAGGTCCGCGAGAAAGGCTGGGTGCTGTCCGCGTACACGATGCCGCCC
AACGCCGAATCGGTCACGAGCCTGCGCGTGGTGGTGCGTCCGCATGTGAACCACAACGTGGCGCAACTGCTTGCCAACGA
TATCATTAACGCCTGCAAGTTCCTCGAGGCCAACGGTGGCAATGCCAAGCCTCCCGCATTGCACAAACATGCCGAAGCGC
AATCGACGTCGTTGAAGTGCTGA

Upstream 100 bases:

>100_bases
GCGCTTTCCGCACCCGTTCGTCTTCCTGTTCGGTGCCTTCGCGCCGTGACCGGGTATCGGGTCGCCGGTTCTCGCCATGT
CAACGTTACGAGGGGTATCC

Downstream 100 bases:

>100_bases
TGAGTGGATGACTGGCCGGCCGAGGGCACGCAATGCCCTCGGCCGGCTTTTTCTTTTTTGGCAACGCCTACGCCAACCGC
AGATCCCGCAACATATCAAG

Product: glutamate decarboxylase B, PLP-dependent

Products: NA

Alternate protein names: GAD [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSDMQEHKVTPYDQFNYEVPENVFPREGISANAAAAIVISDEWTDTNPMLNMSSFVTTFAEPEAREVAARNIFKNYIDHD
MYPRLFAMEGRMVRWLHELWNGPKGVEPYGTCTVGSSEACMLGGLAHKWNWRQRRQAEGKDATRPNMVTGGNVQIVWKKF
LRYFDVEPRIVPLKPGNYCLTAEDLDKYVDENTICVVAIAGQTFTGEDDDIQGIHDWLDAYEKRTGISIPMHIDGASGGF
VNPFLYPDYKWDFRLPRVQSINASGHKYGLTPPGLGWVVFRERKIFNEELVFYVNYLGGEMPTATLNFSRNAFQVAVQYY
QFLRLGFDGYKRVMQHTLDNAIFLRQQLVDSGYFDIMNTTQRIPVVAVTLNKKYKNFNEFDVSNKVREKGWVLSAYTMPP
NAESVTSLRVVVRPHVNHNVAQLLANDIINACKFLEANGGNAKPPALHKHAEAQSTSLKC

Sequences:

>Translated_460_residues
MSDMQEHKVTPYDQFNYEVPENVFPREGISANAAAAIVISDEWTDTNPMLNMSSFVTTFAEPEAREVAARNIFKNYIDHD
MYPRLFAMEGRMVRWLHELWNGPKGVEPYGTCTVGSSEACMLGGLAHKWNWRQRRQAEGKDATRPNMVTGGNVQIVWKKF
LRYFDVEPRIVPLKPGNYCLTAEDLDKYVDENTICVVAIAGQTFTGEDDDIQGIHDWLDAYEKRTGISIPMHIDGASGGF
VNPFLYPDYKWDFRLPRVQSINASGHKYGLTPPGLGWVVFRERKIFNEELVFYVNYLGGEMPTATLNFSRNAFQVAVQYY
QFLRLGFDGYKRVMQHTLDNAIFLRQQLVDSGYFDIMNTTQRIPVVAVTLNKKYKNFNEFDVSNKVREKGWVLSAYTMPP
NAESVTSLRVVVRPHVNHNVAQLLANDIINACKFLEANGGNAKPPALHKHAEAQSTSLKC
>Mature_459_residues
SDMQEHKVTPYDQFNYEVPENVFPREGISANAAAAIVISDEWTDTNPMLNMSSFVTTFAEPEAREVAARNIFKNYIDHDM
YPRLFAMEGRMVRWLHELWNGPKGVEPYGTCTVGSSEACMLGGLAHKWNWRQRRQAEGKDATRPNMVTGGNVQIVWKKFL
RYFDVEPRIVPLKPGNYCLTAEDLDKYVDENTICVVAIAGQTFTGEDDDIQGIHDWLDAYEKRTGISIPMHIDGASGGFV
NPFLYPDYKWDFRLPRVQSINASGHKYGLTPPGLGWVVFRERKIFNEELVFYVNYLGGEMPTATLNFSRNAFQVAVQYYQ
FLRLGFDGYKRVMQHTLDNAIFLRQQLVDSGYFDIMNTTQRIPVVAVTLNKKYKNFNEFDVSNKVREKGWVLSAYTMPPN
AESVTSLRVVVRPHVNHNVAQLLANDIINACKFLEANGGNAKPPALHKHAEAQSTSLKC

Specific function: Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=365, Percent_Identity=25.4794520547945, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1787769, Length=418, Percent_Identity=41.1483253588517, Blast_Score=331, Evalue=7e-92,
Organism=Escherichia coli, GI1789934, Length=418, Percent_Identity=41.1483253588517, Blast_Score=331, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI17557272, Length=378, Percent_Identity=26.1904761904762, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17543922, Length=371, Percent_Identity=24.7978436657682, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25148342, Length=348, Percent_Identity=25.8620689655172, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323905, Length=452, Percent_Identity=37.3893805309735, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6320500, Length=339, Percent_Identity=25.9587020648968, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21355963, Length=338, Percent_Identity=26.9230769230769, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24654344, Length=338, Percent_Identity=26.9230769230769, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 52339; Mature: 52208

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMQEHKVTPYDQFNYEVPENVFPREGISANAAAAIVISDEWTDTNPMLNMSSFVTTFA
CCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHC
EPEAREVAARNIFKNYIDHDMYPRLFAMEGRMVRWLHELWNGPKGVEPYGTCTVGSSEAC
CCHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCH
MLGGLAHKWNWRQRRQAEGKDATRPNMVTGGNVQIVWKKFLRYFDVEPRIVPLKPGNYCL
HHCCCHHCCCCHHHHHCCCCCCCCCCEEECCCEEHHHHHHHHHHCCCCCEEEECCCCEEE
TAEDLDKYVDENTICVVAIAGQTFTGEDDDIQGIHDWLDAYEKRTGISIPMHIDGASGGF
EHHHHHHHCCCCCEEEEEEECCEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
VNPFLYPDYKWDFRLPRVQSINASGHKYGLTPPGLGWVVFRERKIFNEELVFYVNYLGGE
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECHHHCCHHHEEEEEECCCC
MPTATLNFSRNAFQVAVQYYQFLRLGFDGYKRVMQHTLDNAIFLRQQLVDSGYFDIMNTT
CCEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
QRIPVVAVTLNKKYKNFNEFDVSNKVREKGWVLSAYTMPPNAESVTSLRVVVRPHVNHNV
CCCCEEEEEECCCCCCCCCCCCHHHHHHCCEEEEEEECCCCCCCCEEEEEEEECCCCHHH
AQLLANDIINACKFLEANGGNAKPPALHKHAEAQSTSLKC
HHHHHHHHHHHHHHEECCCCCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
SDMQEHKVTPYDQFNYEVPENVFPREGISANAAAAIVISDEWTDTNPMLNMSSFVTTFA
CCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHC
EPEAREVAARNIFKNYIDHDMYPRLFAMEGRMVRWLHELWNGPKGVEPYGTCTVGSSEAC
CCHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCH
MLGGLAHKWNWRQRRQAEGKDATRPNMVTGGNVQIVWKKFLRYFDVEPRIVPLKPGNYCL
HHCCCHHCCCCHHHHHCCCCCCCCCCEEECCCEEHHHHHHHHHHCCCCCEEEECCCCEEE
TAEDLDKYVDENTICVVAIAGQTFTGEDDDIQGIHDWLDAYEKRTGISIPMHIDGASGGF
EHHHHHHHCCCCCEEEEEEECCEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
VNPFLYPDYKWDFRLPRVQSINASGHKYGLTPPGLGWVVFRERKIFNEELVFYVNYLGGE
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECHHHCCHHHEEEEEECCCC
MPTATLNFSRNAFQVAVQYYQFLRLGFDGYKRVMQHTLDNAIFLRQQLVDSGYFDIMNTT
CCEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
QRIPVVAVTLNKKYKNFNEFDVSNKVREKGWVLSAYTMPPNAESVTSLRVVVRPHVNHNV
CCCCEEEEEECCCCCCCCCCCCHHHHHHCCEEEEEEECCCCCCCCEEEEEEEECCCCHHH
AQLLANDIINACKFLEANGGNAKPPALHKHAEAQSTSLKC
HHHHHHHHHHHHHHEECCCCCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10411264; 11337471 [H]