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The map label for this gene is paaF [C]

Identifier: 94310663

GI number: 94310663

Start: 1866233

End: 1867066

Strand: Direct

Name: paaF [C]

Synonym: Rmet_1723

Alternate gene names: 94310663

Gene position: 1866233-1867066 (Clockwise)

Preceding gene: 94310662

Following gene: 94310664

Centisome position: 47.51

GC content: 67.03

Gene sequence:

>834_bases
ATGACCAACCCAGTCATCGTACGTTGCGTGGAGGGCGTGTTGTTCGCCACGCTCAACATCCCGGCCACGCGCAATGCGCT
CGCTCCGGAAGTGGTCGCCGTGCTGACGGACATCGTGGCGCAGGCTGAAGCCGACGTCGGCATCCGCGCGCTGGTGCTAC
GCGGTGCGGGTGGAACCTTCAGCGCGGGAGGCAACGTCGGCAATTTCCAGGCGCGGCTTGACGCCGATGTTGGCCACGAA
GACCCGGTTGCCGCGCGCAACCGCCAGTTCGGTCATTTCATGACACGGCTGGCGACACTCCCGATACCCGTGATCGCGGC
CGTGGACGGGCCGGCGATGGGCGGCGGCATGGGCATGGCATGTGCCGCAGACGTTGTGCTCGCAACGCGCGCGGCGCGGT
TCGCCTTGTCGGAAACCTCGCTGGGCATCATTCCCGCACAGATCGCACCGTTCGTCGTCGGCCGTCTCGGCGCCGCGCTG
ACACGGCGCCTGGGCCTGACCGGCGAGCGTCTGGACGGCGTGGCGGCAGAGGCAGTCGGGCTCGTGGACCAGTTGGCCGA
TGACGCCGCCGGACTGGACGAACTGATTGCCGAGTGGCTGACCCGCATTGGCCGCTGCGGGCCACACGCCAACCGACAGT
TCAAGGCATTGGTCGCCAGATGTGGCCAGACGCCGGTGGACAGCCTGCTTGATGACGCATCGCATCAGTTTGCCCAGTGC
ATGCGCACCGAAGGCACAGAAGGCATCGCGGCCTTCCGCGAAAAGCGCGAAGCGCGCTGGGTCACGCGATTTGATGCCGG
CATCGTATGCGCCGCGCAATCGACAGACGCCTGA

Upstream 100 bases:

>100_bases
TCCGGCTTGGATCGATCGGTGGTGGCGCCGACGAGGTGATGCTGCAAGTTATCGCCAAGCAGATGGGGCTGATGTCCCGC
CACGACCGCTGAGACGAGCC

Downstream 100 bases:

>100_bases
ACGCGAGAAACTTTCCAGAGAGCATTCCATGTCAGCTTCCTGCCGACCCTTCCATACCGTGCTCGTGGCCAACCGGGGCG
AGATCGCCATCCGCATCATG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTNPVIVRCVEGVLFATLNIPATRNALAPEVVAVLTDIVAQAEADVGIRALVLRGAGGTFSAGGNVGNFQARLDADVGHE
DPVAARNRQFGHFMTRLATLPIPVIAAVDGPAMGGGMGMACAADVVLATRAARFALSETSLGIIPAQIAPFVVGRLGAAL
TRRLGLTGERLDGVAAEAVGLVDQLADDAAGLDELIAEWLTRIGRCGPHANRQFKALVARCGQTPVDSLLDDASHQFAQC
MRTEGTEGIAAFREKREARWVTRFDAGIVCAAQSTDA

Sequences:

>Translated_277_residues
MTNPVIVRCVEGVLFATLNIPATRNALAPEVVAVLTDIVAQAEADVGIRALVLRGAGGTFSAGGNVGNFQARLDADVGHE
DPVAARNRQFGHFMTRLATLPIPVIAAVDGPAMGGGMGMACAADVVLATRAARFALSETSLGIIPAQIAPFVVGRLGAAL
TRRLGLTGERLDGVAAEAVGLVDQLADDAAGLDELIAEWLTRIGRCGPHANRQFKALVARCGQTPVDSLLDDASHQFAQC
MRTEGTEGIAAFREKREARWVTRFDAGIVCAAQSTDA
>Mature_276_residues
TNPVIVRCVEGVLFATLNIPATRNALAPEVVAVLTDIVAQAEADVGIRALVLRGAGGTFSAGGNVGNFQARLDADVGHED
PVAARNRQFGHFMTRLATLPIPVIAAVDGPAMGGGMGMACAADVVLATRAARFALSETSLGIIPAQIAPFVVGRLGAALT
RRLGLTGERLDGVAAEAVGLVDQLADDAAGLDELIAEWLTRIGRCGPHANRQFKALVARCGQTPVDSLLDDASHQFAQCM
RTEGTEGIAAFREKREARWVTRFDAGIVCAAQSTDA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI4502327, Length=263, Percent_Identity=30.4182509505703, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1787659, Length=254, Percent_Identity=30.3149606299213, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788597, Length=255, Percent_Identity=28.2352941176471, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787660, Length=275, Percent_Identity=29.8181818181818, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=27.027027027027, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790281, Length=139, Percent_Identity=33.0935251798561, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=259, Percent_Identity=30.1158301158301, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=261, Percent_Identity=26.0536398467433, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=236, Percent_Identity=27.1186440677966, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24653139, Length=261, Percent_Identity=29.1187739463602, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=29.4573643410853, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=29.4573643410853, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=25.6704980842912, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28852; Mature: 28721

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPVIVRCVEGVLFATLNIPATRNALAPEVVAVLTDIVAQAEADVGIRALVLRGAGGTF
CCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
SAGGNVGNFQARLDADVGHEDPVAARNRQFGHFMTRLATLPIPVIAAVDGPAMGGGMGMA
CCCCCCCCCCEEECCCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHH
CAADVVLATRAARFALSETSLGIIPAQIAPFVVGRLGAALTRRLGLTGERLDGVAAEAVG
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LVDQLADDAAGLDELIAEWLTRIGRCGPHANRQFKALVARCGQTPVDSLLDDASHQFAQC
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MRTEGTEGIAAFREKREARWVTRFDAGIVCAAQSTDA
HHCCCCHHHHHHHHHHHHHHHHEECCCEEEECCCCCC
>Mature Secondary Structure 
TNPVIVRCVEGVLFATLNIPATRNALAPEVVAVLTDIVAQAEADVGIRALVLRGAGGTF
CCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
SAGGNVGNFQARLDADVGHEDPVAARNRQFGHFMTRLATLPIPVIAAVDGPAMGGGMGMA
CCCCCCCCCCEEECCCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHH
CAADVVLATRAARFALSETSLGIIPAQIAPFVVGRLGAALTRRLGLTGERLDGVAAEAVG
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LVDQLADDAAGLDELIAEWLTRIGRCGPHANRQFKALVARCGQTPVDSLLDDASHQFAQC
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MRTEGTEGIAAFREKREARWVTRFDAGIVCAAQSTDA
HHCCCCHHHHHHHHHHHHHHHHEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA