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The map label for this gene is acrC [H]

Identifier: 94310643

GI number: 94310643

Start: 1846301

End: 1847761

Strand: Direct

Name: acrC [H]

Synonym: Rmet_1703

Alternate gene names: 94310643

Gene position: 1846301-1847761 (Clockwise)

Preceding gene: 94310642

Following gene: 94310644

Centisome position: 47.0

GC content: 65.3

Gene sequence:

>1461_bases
ATGAAGCGCCGCACGGTGTCCCGATTGATGCTTCTCGCCACGCTGGCCGCCGCCGGGTGCGCGGTCGGGCCGGACTACGT
TCGTCCGCCTGTCGATGTTCCGCAGGCCTACCGCTTCGCGGACGAAGGTATCAGCGTGCCGCAGACACCGGCCACGTATT
GGTGGTCGCAGATGAACGATCCGGTGCTGGACGGGCTGATTGCCACAGCGCGGGCCAACAACAAGGACTTGCTGATTGCC
GCGGCACGGGTCGAGGAGTACTACGGCCGTGTCATGACCACCCGGTCTGGCCTGTTCCCTCAGGTGGGAATCGAGGCTGG
CGGTGGGCGCCAGCGCATCAGCACCACCAATGGCACGGCGCTGCCGATCAACAACCCGTTCAACCAGACGCACGTCGCCG
CCACCGCATCCTGGGAAATCGACCTGTTTGGCCGGATACGCCGCCTGACCGAGGCGGCCAAGGCTGACTGGCAGGCCAGC
GAACTGGCGCGTCGGAGCACGGAAATCTCGGTGGTCGCCGCGACTGCCAGCGCCTATATCACGCTGCGGGATCTCGATAA
CCGCCTGCGTATCTCGCAAGAAACCCTGGAAAGCCGTCGGGGCGCGCTTGAGTTGTTCCGGGAGCGCTTCCAGGGCGGCG
TGGTCTCCGAGGTCGAGTTCGCGCAAGCGCGGTCGGAATACGCATCGGCACTGACCTCGGTCGAGCAGTTCCGCCAGCAA
GTCGCGCTGCAGGAGGATCTGCTGGCCGGTCTGCTGGGCGAGAATCCGCGTGAAATTCCGCGCGGCAAGTCGATCTCCGA
GTTGACCTTGCCGGGCGTGCCGGTCGGCCTGCCTTCGACGTTACTGGAGCAGCGGCCGGACGTGCTCCTGGCCGAGCAGA
ATCTCGTGGCGGCCAATGCCCGCATCGGCGCCGCGCGTGCCGAATACTTCCCGACGATTTCGCTGACCGGCGCGTTCGGC
GCTGCCAGCACGGCACTGTCGGGGCTGTGGACCGGTCCGGCACGGGCCTGGTCGTTCGCAGGGCTCATCAGTATGCCGAT
CTTCACCGGTGGCGCGATCGCCGGCGACGTCAAGCAGGCGGAGGCGATTCAGACGCAGTCGCTGCAGGCCTACCAGCAAT
CCATCCAGACAGCGTTCCGGGAAGTGGCGGATGCGCTGGTCAGCGTGCAGCGTACGCGCGATCAGCTCAATGCCAAGACC
GACCAGGTCGATGCGCTGGGCCGTTACGCTTCGCTGGCACGAGATCGCTACGAAGGCGGTTATACGAGCTATCTTGAAGT
GCTGGATTCCGAGCGCGCGCTGTTCGCCGCGCAACTCGACCAGTCCACCCTCATTGGTTCGGAGTTTGGCCAACTTGTCC
AGCTCTACAAGGCGCTGGGTGGTGGCTGGCCCGTGGAGTCGGAAGCAGCACAGGCGCCTCAGGCAAGCCTGAGTCAACCG
GAAACCCGTAACAAGGAGTGA

Upstream 100 bases:

>100_bases
GCAGCGAGAAGAGGAAGGGCAAGCAGCCGGCTTCAGGGGCTGGGACCGGGCCAGGGGCGGGGCCGGCGAGTGACACCGAG
GCTACTCCGGGAGGCACGTC

Downstream 100 bases:

>100_bases
TTTCATGTCGAGGTCGATCATCAGGCCGGTCTTGTTGCTGGCCACGGCGGGCATCGCCCTTGGTGCGATGGCCCAGTCCA
AGCCGATTGCGTATCCCGCC

Product: cation/multidrug efflux system outer membrane porin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MKRRTVSRLMLLATLAAAGCAVGPDYVRPPVDVPQAYRFADEGISVPQTPATYWWSQMNDPVLDGLIATARANNKDLLIA
AARVEEYYGRVMTTRSGLFPQVGIEAGGGRQRISTTNGTALPINNPFNQTHVAATASWEIDLFGRIRRLTEAAKADWQAS
ELARRSTEISVVAATASAYITLRDLDNRLRISQETLESRRGALELFRERFQGGVVSEVEFAQARSEYASALTSVEQFRQQ
VALQEDLLAGLLGENPREIPRGKSISELTLPGVPVGLPSTLLEQRPDVLLAEQNLVAANARIGAARAEYFPTISLTGAFG
AASTALSGLWTGPARAWSFAGLISMPIFTGGAIAGDVKQAEAIQTQSLQAYQQSIQTAFREVADALVSVQRTRDQLNAKT
DQVDALGRYASLARDRYEGGYTSYLEVLDSERALFAAQLDQSTLIGSEFGQLVQLYKALGGGWPVESEAAQAPQASLSQP
ETRNKE

Sequences:

>Translated_486_residues
MKRRTVSRLMLLATLAAAGCAVGPDYVRPPVDVPQAYRFADEGISVPQTPATYWWSQMNDPVLDGLIATARANNKDLLIA
AARVEEYYGRVMTTRSGLFPQVGIEAGGGRQRISTTNGTALPINNPFNQTHVAATASWEIDLFGRIRRLTEAAKADWQAS
ELARRSTEISVVAATASAYITLRDLDNRLRISQETLESRRGALELFRERFQGGVVSEVEFAQARSEYASALTSVEQFRQQ
VALQEDLLAGLLGENPREIPRGKSISELTLPGVPVGLPSTLLEQRPDVLLAEQNLVAANARIGAARAEYFPTISLTGAFG
AASTALSGLWTGPARAWSFAGLISMPIFTGGAIAGDVKQAEAIQTQSLQAYQQSIQTAFREVADALVSVQRTRDQLNAKT
DQVDALGRYASLARDRYEGGYTSYLEVLDSERALFAAQLDQSTLIGSEFGQLVQLYKALGGGWPVESEAAQAPQASLSQP
ETRNKE
>Mature_486_residues
MKRRTVSRLMLLATLAAAGCAVGPDYVRPPVDVPQAYRFADEGISVPQTPATYWWSQMNDPVLDGLIATARANNKDLLIA
AARVEEYYGRVMTTRSGLFPQVGIEAGGGRQRISTTNGTALPINNPFNQTHVAATASWEIDLFGRIRRLTEAAKADWQAS
ELARRSTEISVVAATASAYITLRDLDNRLRISQETLESRRGALELFRERFQGGVVSEVEFAQARSEYASALTSVEQFRQQ
VALQEDLLAGLLGENPREIPRGKSISELTLPGVPVGLPSTLLEQRPDVLLAEQNLVAANARIGAARAEYFPTISLTGAFG
AASTALSGLWTGPARAWSFAGLISMPIFTGGAIAGDVKQAEAIQTQSLQAYQQSIQTAFREVADALVSVQRTRDQLNAKT
DQVDALGRYASLARDRYEGGYTSYLEVLDSERALFAAQLDQSTLIGSEFGQLVQLYKALGGGWPVESEAAQAPQASLSQP
ETRNKE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=466, Percent_Identity=33.4763948497854, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI2367348, Length=482, Percent_Identity=25.103734439834, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI87082199, Length=447, Percent_Identity=23.9373601789709, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52658; Mature: 52658

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRTVSRLMLLATLAAAGCAVGPDYVRPPVDVPQAYRFADEGISVPQTPATYWWSQMND
CCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCC
PVLDGLIATARANNKDLLIAAARVEEYYGRVMTTRSGLFPQVGIEAGGGRQRISTTNGTA
HHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCE
LPINNPFNQTHVAATASWEIDLFGRIRRLTEAAKADWQASELARRSTEISVVAATASAYI
EECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEEEEEHEE
TLRDLDNRLRISQETLESRRGALELFRERFQGGVVSEVEFAQARSEYASALTSVEQFRQQ
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VALQEDLLAGLLGENPREIPRGKSISELTLPGVPVGLPSTLLEQRPDVLLAEQNLVAANA
HHHHHHHHHHHHCCCHHHCCCCCCCCCEECCCCCCCCCHHHHHCCCCEEEECCCEEEECC
RIGAARAEYFPTISLTGAFGAASTALSGLWTGPARAWSFAGLISMPIFTGGAIAGDVKQA
CCCHHHHHHCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
EAIQTQSLQAYQQSIQTAFREVADALVSVQRTRDQLNAKTDQVDALGRYASLARDRYEGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
YTSYLEVLDSERALFAAQLDQSTLIGSEFGQLVQLYKALGGGWPVESEAAQAPQASLSQP
HHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHCCCC
ETRNKE
CCCCCC
>Mature Secondary Structure
MKRRTVSRLMLLATLAAAGCAVGPDYVRPPVDVPQAYRFADEGISVPQTPATYWWSQMND
CCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCC
PVLDGLIATARANNKDLLIAAARVEEYYGRVMTTRSGLFPQVGIEAGGGRQRISTTNGTA
HHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCE
LPINNPFNQTHVAATASWEIDLFGRIRRLTEAAKADWQASELARRSTEISVVAATASAYI
EECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEEEEEHEE
TLRDLDNRLRISQETLESRRGALELFRERFQGGVVSEVEFAQARSEYASALTSVEQFRQQ
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VALQEDLLAGLLGENPREIPRGKSISELTLPGVPVGLPSTLLEQRPDVLLAEQNLVAANA
HHHHHHHHHHHHCCCHHHCCCCCCCCCEECCCCCCCCCHHHHHCCCCEEEECCCEEEECC
RIGAARAEYFPTISLTGAFGAASTALSGLWTGPARAWSFAGLISMPIFTGGAIAGDVKQA
CCCHHHHHHCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
EAIQTQSLQAYQQSIQTAFREVADALVSVQRTRDQLNAKTDQVDALGRYASLARDRYEGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
YTSYLEVLDSERALFAAQLDQSTLIGSEFGQLVQLYKALGGGWPVESEAAQAPQASLSQP
HHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHCCCC
ETRNKE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]