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The map label for this gene is acrA [H]

Identifier: 94310641

GI number: 94310641

Start: 1841878

End: 1843116

Strand: Direct

Name: acrA [H]

Synonym: Rmet_1701

Alternate gene names: 94310641

Gene position: 1841878-1843116 (Clockwise)

Preceding gene: 94310640

Following gene: 94310642

Centisome position: 46.89

GC content: 66.02

Gene sequence:

>1239_bases
ATGGCGAGTGGACTGGAGTCCAAGGGCAGGCAGTATGCGTTCATGTTCCTCACCGCACTGGCAGTAGCGGGATGCGGAAA
GCAGGAGGCGAAGGGGCCGGCCGCGCCGCCCGTGACCGAGGTTGGGGTCGTCAAGGTCGACCTCCACGACGTACCCGTCG
CGTTCGAGTTCGTCGGGCAGACCGAGAGCTCGCAGCAGGTTGAAATCCGGGCGCGGGTCAACGGTTTCCTGGAGCAACGG
CTCTATACCGAAGGCACGATGGTCAAGGCGGGGCAGGTGCTGTTCCGCATGGACCAGAAGCCATTCAAGGCCGCGCTGGA
CGCGGCCATGGCCGAACTGGCGCAGCAACAGGCGCGCCTCACCACCGCGCGCCAGAACCTTGCCCGCGTGCGCCCGCTGG
CGGCCAAGAATGCGTTGAGCCAGAAGGATCTCGACGATTCCGTTGGGCAGGAGCAGGGCGCCGCCGCCGCGGTGGAGCAG
GCCAAGGCCAACGTGACAAACGCCCGTCTGAATCTGGACTACACGACGATCACCGCGCCGGTCTCCGGGTTATCCAGCTA
CGCCAAGAAGCAGGTCGGTTCGTATATCGACGCGAGCAACAGCTTGCTGACTTATGTCGCCAAGCTCGACCCGATCTGGG
TCAATTTCAGTTTGTCGGAGAACGAGGTCCTCCAGTACCAGTCCCAGCAAGCCTCCGGGCAGTTGAAGATGCCGCCGCGC
GGCTCGATGGAGGTGGAGATCGTCTTGGCGGATGGCGCGACCTATGGCAAGCGTGGCCACATCACGTTTGCCGATGCATC
GTTCAGCTCCGAGACTGGCACCTATCTGCTGCGCGCGGAATTCCCGAATACCGAAGGCGCGCTGCGTCCGGGGCAATTCG
TGCGCGTTCGCGTGCTTGGCGCGGAGCGCACGCGCGCGATCACGGTGCCGCAGGCGGCCGTGGTGCAAGGGCAACGTGGA
CAGTTTGTCTGGACCGTCGGCAAGGACAACAAGGCAGAGACCAGAGTGGTGGATGTCGGTGAATGGGCGCAGGGCAACTG
GATTATCCGCTCCGGCCTGCGCGAAGGCGATACCGTGGTGGTCGATGGCACGGTGCGCCTCGCTCCCGGCGCGCCCCTCA
AGCCAGTGGCCGCGCCTCCCCAGCCGGTGTCCACCGAACGGCCGCCTGGCTCCCCCGGCGCCGCGCCCACGCCAGCCTCG
GCCCCAACCGCCGCCCCTGAGAAGGGGGCGACGTCATGA

Upstream 100 bases:

>100_bases
TTGAGAGAACGCTTCAACTGACCACGCAGGCAACACATCAACCACGCAGACGGCGAGGACGCGCCAGCAAGCAGGCGGGC
GCGTAGCGGAGGAGCAAGCA

Downstream 100 bases:

>100_bases
AGCTTTCACACTTCTTCATCGACCGTCCGATCTTCGCATCGGTCCTGTCGATCATCATTGTCGTTGGCGGTCTGGTTGCC
ATGGTCAACCTGCCGATCGC

Product: cation/multidrug efflux system, mebrane-fusion component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MASGLESKGRQYAFMFLTALAVAGCGKQEAKGPAAPPVTEVGVVKVDLHDVPVAFEFVGQTESSQQVEIRARVNGFLEQR
LYTEGTMVKAGQVLFRMDQKPFKAALDAAMAELAQQQARLTTARQNLARVRPLAAKNALSQKDLDDSVGQEQGAAAAVEQ
AKANVTNARLNLDYTTITAPVSGLSSYAKKQVGSYIDASNSLLTYVAKLDPIWVNFSLSENEVLQYQSQQASGQLKMPPR
GSMEVEIVLADGATYGKRGHITFADASFSSETGTYLLRAEFPNTEGALRPGQFVRVRVLGAERTRAITVPQAAVVQGQRG
QFVWTVGKDNKAETRVVDVGEWAQGNWIIRSGLREGDTVVVDGTVRLAPGAPLKPVAAPPQPVSTERPPGSPGAAPTPAS
APTAAPEKGATS

Sequences:

>Translated_412_residues
MASGLESKGRQYAFMFLTALAVAGCGKQEAKGPAAPPVTEVGVVKVDLHDVPVAFEFVGQTESSQQVEIRARVNGFLEQR
LYTEGTMVKAGQVLFRMDQKPFKAALDAAMAELAQQQARLTTARQNLARVRPLAAKNALSQKDLDDSVGQEQGAAAAVEQ
AKANVTNARLNLDYTTITAPVSGLSSYAKKQVGSYIDASNSLLTYVAKLDPIWVNFSLSENEVLQYQSQQASGQLKMPPR
GSMEVEIVLADGATYGKRGHITFADASFSSETGTYLLRAEFPNTEGALRPGQFVRVRVLGAERTRAITVPQAAVVQGQRG
QFVWTVGKDNKAETRVVDVGEWAQGNWIIRSGLREGDTVVVDGTVRLAPGAPLKPVAAPPQPVSTERPPGSPGAAPTPAS
APTAAPEKGATS
>Mature_411_residues
ASGLESKGRQYAFMFLTALAVAGCGKQEAKGPAAPPVTEVGVVKVDLHDVPVAFEFVGQTESSQQVEIRARVNGFLEQRL
YTEGTMVKAGQVLFRMDQKPFKAALDAAMAELAQQQARLTTARQNLARVRPLAAKNALSQKDLDDSVGQEQGAAAAVEQA
KANVTNARLNLDYTTITAPVSGLSSYAKKQVGSYIDASNSLLTYVAKLDPIWVNFSLSENEVLQYQSQQASGQLKMPPRG
SMEVEIVLADGATYGKRGHITFADASFSSETGTYLLRAEFPNTEGALRPGQFVRVRVLGAERTRAITVPQAAVVQGQRGQ
FVWTVGKDNKAETRVVDVGEWAQGNWIIRSGLREGDTVVVDGTVRLAPGAPLKPVAAPPQPVSTERPPGSPGAAPTPASA
PTAAPEKGATS

Specific function: Involved in resistance to several unrelated toxic compounds, such as dyes, detergents and antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=376, Percent_Identity=33.2446808510638, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1789665, Length=371, Percent_Identity=33.4231805929919, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1786668, Length=365, Percent_Identity=33.4246575342466, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87082036, Length=371, Percent_Identity=32.0754716981132, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI87081951, Length=143, Percent_Identity=31.4685314685315, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081796, Length=324, Percent_Identity=26.8518518518519, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43792; Mature: 43661

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGLESKGRQYAFMFLTALAVAGCGKQEAKGPAAPPVTEVGVVKVDLHDVPVAFEFVGQ
CCCCCCCCCCEEHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCEEEEECCCCCEEEEEECC
TESSQQVEIRARVNGFLEQRLYTEGTMVKAGQVLFRMDQKPFKAALDAAMAELAQQQARL
CCCCCEEEEEHHHHHHHHHHHHCCCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
TTARQNLARVRPLAAKNALSQKDLDDSVGQEQGAAAAVEQAKANVTNARLNLDYTTITAP
HHHHHHHHHHCCHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEECC
VSGLSSYAKKQVGSYIDASNSLLTYVAKLDPIWVNFSLSENEVLQYQSQQASGQLKMPPR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCEEECCCC
GSMEVEIVLADGATYGKRGHITFADASFSSETGTYLLRAEFPNTEGALRPGQFVRVRVLG
CCEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEC
AERTRAITVPQAAVVQGQRGQFVWTVGKDNKAETRVVDVGEWAQGNWIIRSGLREGDTVV
CCCCEEEECCHHHHEECCCCCEEEEECCCCCCCEEEEECCCCCCCCEEEECCCCCCCEEE
VDGTVRLAPGAPLKPVAAPPQPVSTERPPGSPGAAPTPASAPTAAPEKGATS
EECEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASGLESKGRQYAFMFLTALAVAGCGKQEAKGPAAPPVTEVGVVKVDLHDVPVAFEFVGQ
CCCCCCCCCEEHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCEEEEECCCCCEEEEEECC
TESSQQVEIRARVNGFLEQRLYTEGTMVKAGQVLFRMDQKPFKAALDAAMAELAQQQARL
CCCCCEEEEEHHHHHHHHHHHHCCCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
TTARQNLARVRPLAAKNALSQKDLDDSVGQEQGAAAAVEQAKANVTNARLNLDYTTITAP
HHHHHHHHHHCCHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEECC
VSGLSSYAKKQVGSYIDASNSLLTYVAKLDPIWVNFSLSENEVLQYQSQQASGQLKMPPR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCEEECCCC
GSMEVEIVLADGATYGKRGHITFADASFSSETGTYLLRAEFPNTEGALRPGQFVRVRVLG
CCEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEC
AERTRAITVPQAAVVQGQRGQFVWTVGKDNKAETRVVDVGEWAQGNWIIRSGLREGDTVV
CCCCEEEECCHHHHEECCCCCEEEEECCCCCCCEEEEECCCCCCCCEEEECCCCCCCEEE
VDGTVRLAPGAPLKPVAAPPQPVSTERPPGSPGAAPTPASAPTAAPEKGATS
EECEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]