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The map label for this gene is garL [H]

Identifier: 94310575

GI number: 94310575

Start: 1761742

End: 1762527

Strand: Reverse

Name: garL [H]

Synonym: Rmet_1633

Alternate gene names: 94310575

Gene position: 1762527-1761742 (Counterclockwise)

Preceding gene: 94310576

Following gene: 94310574

Centisome position: 44.87

GC content: 64.38

Gene sequence:

>786_bases
ATGACCCAACATCTGCCGCAGATCACCCCCCTGCCAAACCGTTTCCGGGCCGACGTGCTCGCGCGCAAGCGCCTGATTGG
CCTGTGGAGCTCGCTGGGCCAGCCCGTGACCACCGAGATCATCGGCCTGGCCGGATTTGACTGGCTGCTGCTCGACGGAG
AACACTCACCCAATGACGTGCTGACGTTCATGCCGCAACTCATGGCGCTCAAGGACAGCACCAGCGCGCCGGTGGTTCGC
CCGCCGTGGAACGACCCAGTGATGCTCAAGCGGCTGCTCGATATCGGCTTCTACAATTTCCTGATTCCGTTCGTGGAATC
GGCGGAAGAAGCCCGTCGTGCCGTGCGCGCCACGCGCTACCCGCCTGACGGCATCCGTGGCGTCTCCGGCGCGCAGCGCC
AGAACCGCTATGGCACGTTGCCGGACTTCTTCAAGACCATCAACGACAATATCTGCGTGCTGGTACAGATCGAAAGCCGC
AAGGGCGTGGAAGCGGCCGACGAAATCTGCGCGGAACCCGGCGTTGACGGCATCTTCATCGGCCCCAACGACCTGGCCGC
AGCCTACGGGCATCTGGGCAACCCGAACCATCCTGAGGTCCAGGCAGCCGTGCGGCAGATCTATGAAGCGGCCAACCGCG
CCGGCAAGGCCATTGGCACGCTGACGCCCGTGGAAGCCGACGCCAAGCGCTACCTGGACATGGGCGCGCATTTTGTTGCG
GTGGGTATCGACAACGCACTGCTGCGCGACGCGGCACGCGCGTTGCGCGAGCGCTTTACCGTGTGA

Upstream 100 bases:

>100_bases
GGCCCAGCTAGACGATGCCGCCGCTCGCGTGCTTCAGGCGCGTGGCAAGTCGCCGGGGCCGGTCTCGGCGCTGGCGACCG
ATTGAACCAGGAGTAACCGC

Downstream 100 bases:

>100_bases
CTCCCCTTCAACCGGATATCGACATGAGCAAGATCGGATTTATCGGACTCGGCGTAATGGGCAAGCCCATGGCGCTGCAT
CTGCAAACGGGCGGCCACGC

Product: alpha-dehydro-beta-deoxy-D-glucarate aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTQHLPQITPLPNRFRADVLARKRLIGLWSSLGQPVTTEIIGLAGFDWLLLDGEHSPNDVLTFMPQLMALKDSTSAPVVR
PPWNDPVMLKRLLDIGFYNFLIPFVESAEEARRAVRATRYPPDGIRGVSGAQRQNRYGTLPDFFKTINDNICVLVQIESR
KGVEAADEICAEPGVDGIFIGPNDLAAAYGHLGNPNHPEVQAAVRQIYEAANRAGKAIGTLTPVEADAKRYLDMGAHFVA
VGIDNALLRDAARALRERFTV

Sequences:

>Translated_261_residues
MTQHLPQITPLPNRFRADVLARKRLIGLWSSLGQPVTTEIIGLAGFDWLLLDGEHSPNDVLTFMPQLMALKDSTSAPVVR
PPWNDPVMLKRLLDIGFYNFLIPFVESAEEARRAVRATRYPPDGIRGVSGAQRQNRYGTLPDFFKTINDNICVLVQIESR
KGVEAADEICAEPGVDGIFIGPNDLAAAYGHLGNPNHPEVQAAVRQIYEAANRAGKAIGTLTPVEADAKRYLDMGAHFVA
VGIDNALLRDAARALRERFTV
>Mature_260_residues
TQHLPQITPLPNRFRADVLARKRLIGLWSSLGQPVTTEIIGLAGFDWLLLDGEHSPNDVLTFMPQLMALKDSTSAPVVRP
PWNDPVMLKRLLDIGFYNFLIPFVESAEEARRAVRATRYPPDGIRGVSGAQRQNRYGTLPDFFKTINDNICVLVQIESRK
GVEAADEICAEPGVDGIFIGPNDLAAAYGHLGNPNHPEVQAAVRQIYEAANRAGKAIGTLTPVEADAKRYLDMGAHFVAV
GIDNALLRDAARALRERFTV

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=249, Percent_Identity=61.0441767068273, Blast_Score=325, Evalue=2e-90,
Organism=Escherichia coli, GI1788578, Length=242, Percent_Identity=42.5619834710744, Blast_Score=196, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 28722; Mature: 28591

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQHLPQITPLPNRFRADVLARKRLIGLWSSLGQPVTTEIIGLAGFDWLLLDGEHSPNDV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCCHHH
LTFMPQLMALKDSTSAPVVRPPWNDPVMLKRLLDIGFYNFLIPFVESAEEARRAVRATRY
HHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PPDGIRGVSGAQRQNRYGTLPDFFKTINDNICVLVQIESRKGVEAADEICAEPGVDGIFI
CCCCCCCCCCHHHHCCCCCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEE
GPNDLAAAYGHLGNPNHPEVQAAVRQIYEAANRAGKAIGTLTPVEADAKRYLDMGAHFVA
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHCCCEEEE
VGIDNALLRDAARALRERFTV
EECCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQHLPQITPLPNRFRADVLARKRLIGLWSSLGQPVTTEIIGLAGFDWLLLDGEHSPNDV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCCHHH
LTFMPQLMALKDSTSAPVVRPPWNDPVMLKRLLDIGFYNFLIPFVESAEEARRAVRATRY
HHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PPDGIRGVSGAQRQNRYGTLPDFFKTINDNICVLVQIESRKGVEAADEICAEPGVDGIFI
CCCCCCCCCCHHHHCCCCCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEE
GPNDLAAAYGHLGNPNHPEVQAAVRQIYEAANRAGKAIGTLTPVEADAKRYLDMGAHFVA
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHCCCEEEE
VGIDNALLRDAARALRERFTV
EECCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA