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The map label for this gene is ilvG [H]

Identifier: 94310562

GI number: 94310562

Start: 1746621

End: 1748324

Strand: Direct

Name: ilvG [H]

Synonym: Rmet_1620

Alternate gene names: 94310562

Gene position: 1746621-1748324 (Clockwise)

Preceding gene: 94310561

Following gene: 94310563

Centisome position: 44.46

GC content: 66.78

Gene sequence:

>1704_bases
ATGAGCGCGCCACGCGAACGGAATGGCGGCCAGATTCTGGTCGAACAGCTGCGCATCCAGGGCACGAAGCGCGTGTTCCT
GGTGCCGGGCGAGAGCTATCTGCCGTGCATCGACGCGCTCTACGACCATCAGGATGCGATCACGCCGATCGTCTGCCGGC
AGGAGAGCGGCGCCGGATACATGGCGGAGGCCTACGGCAAGCTGACCGGCGAGCCCGGCGTCTGTTTTGTCACGCGCGGC
CCGGGGGCGACCAATGCGAGCATCGCGGTGCATACGGCGTTTCAGGACTCCACACCGATGATCCTGTTCGTCGGTCAGGT
TGGAAACGACTTCTACGAGCGCGAGGCATTCCAGGAGATCGACTACCGTCGCATGTTCGGCCAGATGGCCAAGTGGGTGG
CCCAGATCGATCGCACGGACCGGATCCCCGAGTTCGTGGCGCGAGCCTACGCGGTCGCAACCAGCGGGCGTCCCGGTCCG
GTGGTGCTGGCGTTGCCCGAGGACACGCTCTGGGGCCGCGCCACGGTCGCCGACATGCCGCGTTATGAGCGCGTCCACGG
CGCCCCGACGCCGGTGGCGCTCGACAAGCTCTCCACCTTGCTGGCTGGCGCGAAGCGGCCGTTCCTGCTGGTCGGGGGCT
CGGGCTGGACGCCCACGGCCATGCGGCAGATCGAAGGCTTTGCCGAACGCTTCGGCCTGCCCGTGGGTGTGGCATGGCGA
CGTCTGGAATGCTTCGACAACGGACATCCGAACTTCGCCGGCCACGTCGGTTGGGGGATGGTCGATGCGCTGCGCCAGCG
GATTCACGAAGCGGATCTGGTGATCGCCGTGGGCACGCGCATGGGCGAGGCCACCACGGAAGGCTACACGCTGGTGGAAA
GCCCGCAGCCACGGCAGAAGCTGGTGCACGTGTATCCCGATCCGGACGAACTCGGCCGGGTATTCCGCCCGACGTTGCCG
ATTGCCGCCGATGTGGTGTCGTTCGCGGCGGCCGTGGCGCAGCTATCTCCGGCGCAGACGGTATCCCGAGAGGCGATGAC
GGCGGTGACGGCGGCCAATGCCAACTTCCTGGCGGAGCAGGAGCCCAAGGACGCCCCCGGCGCGTTGAACCTGAACCGGG
TGGCCTGCCACGTGCGCGACCACGTGCCGGACGATGCCTGCATCACTGTCGGCGCAGGCAACTATGCGTTGTTCGCGCAC
GCCTATCACCGCTTCCATGGCGTGGGCACGAGCCTGGCGCCGACGGTAGGGTCGATGGGTTACGGCTTGCCGGCCGCAAT
CTCGGCCAAGCTCGAGAATCCGTCGCGTACCGTGGTCTGCTACGCGGGCGATGGCTGCTTCCAGATGAACCTGCAGGAAC
TCGGCGTGGCCATGCAGTATCGGCTGGGCATCGTGATCCTTGTTTTCAACAACGGCATGTGGGGCACGATCCGCGCGCAT
CAGGAACGCGAGTTCCCGGGCCGCACGATTGCGCTCGGCTTCGACAATCCCGAGTTCAGCCAGCTGGTCAAGGCGTATCA
CGGCCACGGCGAAGTGGTGGATCGCGATGAGGATTTCGCCCCCGCCTTTGCCCGGGCGCTGGAATTCGCCAATCGCGAGC
AACTCCCCGCGCTGATCGAGCTGCGCTACGAACCCGATGGTATCGCGCCCGGCGTCACGCTCTCCGGCATTCGTGCCGCA
GCGCTGGCGCGGCAATCCGCCTGA

Upstream 100 bases:

>100_bases
GCGCCTATTACCGGGCAGCCGGCGTCGAGCACAACGTGATCAACGCCCATGACGGTGAATGCGTGTTCGTCGAAATCGAA
ATCAAGCGTGGAGACCAGGC

Downstream 100 bases:

>100_bases
GCGACGCAACACGACGACAACCGACGGAACCACCCGTAATTCCCACCATGCGCGCCTTTTTCTCGGATGACCAGTTGCTG
CACCAGCCTCGGCAGTTCAT

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSAPRERNGGQILVEQLRIQGTKRVFLVPGESYLPCIDALYDHQDAITPIVCRQESGAGYMAEAYGKLTGEPGVCFVTRG
PGATNASIAVHTAFQDSTPMILFVGQVGNDFYEREAFQEIDYRRMFGQMAKWVAQIDRTDRIPEFVARAYAVATSGRPGP
VVLALPEDTLWGRATVADMPRYERVHGAPTPVALDKLSTLLAGAKRPFLLVGGSGWTPTAMRQIEGFAERFGLPVGVAWR
RLECFDNGHPNFAGHVGWGMVDALRQRIHEADLVIAVGTRMGEATTEGYTLVESPQPRQKLVHVYPDPDELGRVFRPTLP
IAADVVSFAAAVAQLSPAQTVSREAMTAVTAANANFLAEQEPKDAPGALNLNRVACHVRDHVPDDACITVGAGNYALFAH
AYHRFHGVGTSLAPTVGSMGYGLPAAISAKLENPSRTVVCYAGDGCFQMNLQELGVAMQYRLGIVILVFNNGMWGTIRAH
QEREFPGRTIALGFDNPEFSQLVKAYHGHGEVVDRDEDFAPAFARALEFANREQLPALIELRYEPDGIAPGVTLSGIRAA
ALARQSA

Sequences:

>Translated_567_residues
MSAPRERNGGQILVEQLRIQGTKRVFLVPGESYLPCIDALYDHQDAITPIVCRQESGAGYMAEAYGKLTGEPGVCFVTRG
PGATNASIAVHTAFQDSTPMILFVGQVGNDFYEREAFQEIDYRRMFGQMAKWVAQIDRTDRIPEFVARAYAVATSGRPGP
VVLALPEDTLWGRATVADMPRYERVHGAPTPVALDKLSTLLAGAKRPFLLVGGSGWTPTAMRQIEGFAERFGLPVGVAWR
RLECFDNGHPNFAGHVGWGMVDALRQRIHEADLVIAVGTRMGEATTEGYTLVESPQPRQKLVHVYPDPDELGRVFRPTLP
IAADVVSFAAAVAQLSPAQTVSREAMTAVTAANANFLAEQEPKDAPGALNLNRVACHVRDHVPDDACITVGAGNYALFAH
AYHRFHGVGTSLAPTVGSMGYGLPAAISAKLENPSRTVVCYAGDGCFQMNLQELGVAMQYRLGIVILVFNNGMWGTIRAH
QEREFPGRTIALGFDNPEFSQLVKAYHGHGEVVDRDEDFAPAFARALEFANREQLPALIELRYEPDGIAPGVTLSGIRAA
ALARQSA
>Mature_566_residues
SAPRERNGGQILVEQLRIQGTKRVFLVPGESYLPCIDALYDHQDAITPIVCRQESGAGYMAEAYGKLTGEPGVCFVTRGP
GATNASIAVHTAFQDSTPMILFVGQVGNDFYEREAFQEIDYRRMFGQMAKWVAQIDRTDRIPEFVARAYAVATSGRPGPV
VLALPEDTLWGRATVADMPRYERVHGAPTPVALDKLSTLLAGAKRPFLLVGGSGWTPTAMRQIEGFAERFGLPVGVAWRR
LECFDNGHPNFAGHVGWGMVDALRQRIHEADLVIAVGTRMGEATTEGYTLVESPQPRQKLVHVYPDPDELGRVFRPTLPI
AADVVSFAAAVAQLSPAQTVSREAMTAVTAANANFLAEQEPKDAPGALNLNRVACHVRDHVPDDACITVGAGNYALFAHA
YHRFHGVGTSLAPTVGSMGYGLPAAISAKLENPSRTVVCYAGDGCFQMNLQELGVAMQYRLGIVILVFNNGMWGTIRAHQ
EREFPGRTIALGFDNPEFSQLVKAYHGHGEVVDRDEDFAPAFARALEFANREQLPALIELRYEPDGIAPGVTLSGIRAAA
LARQSA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=572, Percent_Identity=23.9510489510489, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI21361361, Length=559, Percent_Identity=25.2236135957066, Blast_Score=130, Evalue=4e-30,
Organism=Escherichia coli, GI87081685, Length=548, Percent_Identity=29.5620437956204, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=31.0035842293907, Blast_Score=212, Evalue=7e-56,
Organism=Escherichia coli, GI1786717, Length=555, Percent_Identity=27.2072072072072, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788716, Length=482, Percent_Identity=25.3112033195021, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787096, Length=526, Percent_Identity=23.0038022813688, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17542570, Length=560, Percent_Identity=25.3571428571429, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17531299, Length=557, Percent_Identity=24.5960502692998, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531301, Length=557, Percent_Identity=24.5960502692998, Blast_Score=110, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=563, Percent_Identity=28.4191829484902, Blast_Score=203, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6320816, Length=566, Percent_Identity=22.6148409893993, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922626, Length=573, Percent_Identity=25.6544502617801, Blast_Score=121, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61529; Mature: 61397

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRERNGGQILVEQLRIQGTKRVFLVPGESYLPCIDALYDHQDAITPIVCRQESGAGY
CCCCCCCCCCCEEEEHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCCCEEEEECCCCCCC
MAEAYGKLTGEPGVCFVTRGPGATNASIAVHTAFQDSTPMILFVGQVGNDFYEREAFQEI
HHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
DYRRMFGQMAKWVAQIDRTDRIPEFVARAYAVATSGRPGPVVLALPEDTLWGRATVADMP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEHHCCC
RYERVHGAPTPVALDKLSTLLAGAKRPFLLVGGSGWTPTAMRQIEGFAERFGLPVGVAWR
CHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHH
RLECFDNGHPNFAGHVGWGMVDALRQRIHEADLVIAVGTRMGEATTEGYTLVESPQPRQK
EEHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEECCCCCHHH
LVHVYPDPDELGRVFRPTLPIAADVVSFAAAVAQLSPAQTVSREAMTAVTAANANFLAEQ
EEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECC
EPKDAPGALNLNRVACHVRDHVPDDACITVGAGNYALFAHAYHRFHGVGTSLAPTVGSMG
CCCCCCCCCCHHHEEEEECCCCCCCCEEEECCCCEEHHHHHHHHHHCCCCCHHCCCCCCC
YGLPAAISAKLENPSRTVVCYAGDGCFQMNLQELGVAMQYRLGIVILVFNNGMWGTIRAH
CCCCCEEEEECCCCCEEEEEEECCCEEEECHHHHCHHEEEECCEEEEEECCCCEEEEECC
QEREFPGRTIALGFDNPEFSQLVKAYHGHGEVVDRDEDFAPAFARALEFANREQLPALIE
CCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHCCCCCCCEEEE
LRYEPDGIAPGVTLSGIRAAALARQSA
EEECCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAPRERNGGQILVEQLRIQGTKRVFLVPGESYLPCIDALYDHQDAITPIVCRQESGAGY
CCCCCCCCCCEEEEHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCCCEEEEECCCCCCC
MAEAYGKLTGEPGVCFVTRGPGATNASIAVHTAFQDSTPMILFVGQVGNDFYEREAFQEI
HHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
DYRRMFGQMAKWVAQIDRTDRIPEFVARAYAVATSGRPGPVVLALPEDTLWGRATVADMP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEHHCCC
RYERVHGAPTPVALDKLSTLLAGAKRPFLLVGGSGWTPTAMRQIEGFAERFGLPVGVAWR
CHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHH
RLECFDNGHPNFAGHVGWGMVDALRQRIHEADLVIAVGTRMGEATTEGYTLVESPQPRQK
EEHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEECCCCCHHH
LVHVYPDPDELGRVFRPTLPIAADVVSFAAAVAQLSPAQTVSREAMTAVTAANANFLAEQ
EEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECC
EPKDAPGALNLNRVACHVRDHVPDDACITVGAGNYALFAHAYHRFHGVGTSLAPTVGSMG
CCCCCCCCCCHHHEEEEECCCCCCCCEEEECCCCEEHHHHHHHHHHCCCCCHHCCCCCCC
YGLPAAISAKLENPSRTVVCYAGDGCFQMNLQELGVAMQYRLGIVILVFNNGMWGTIRAH
CCCCCEEEEECCCCCEEEEEEECCCEEEECHHHHCHHEEEECCEEEEEECCCCEEEEECC
QEREFPGRTIALGFDNPEFSQLVKAYHGHGEVVDRDEDFAPAFARALEFANREQLPALIE
CCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHCCCCCCCEEEE
LRYEPDGIAPGVTLSGIRAAALARQSA
EEECCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]