Click here to switch to the map view.

The map label for this gene is prpB [H]

Identifier: 94310529

GI number: 94310529

Start: 1710063

End: 1710971

Strand: Reverse

Name: prpB [H]

Synonym: Rmet_1587

Alternate gene names: 94310529

Gene position: 1710971-1710063 (Counterclockwise)

Preceding gene: 94310531

Following gene: 94310528

Centisome position: 43.56

GC content: 65.9

Gene sequence:

>909_bases
ATGACCTTTACCGCCACCGAACTCGCCCGCTCCGCCGGCGCACGCTTCCGCCAGGCCCTGATCGATGAAAGCCCCCTGCA
GATCCCGGGCACCATCAATGCCAATCACGCGCTGCTGGCCAAGCGGGCCGGTTATCGCGCGATCTACCTGTCGGGCGGCG
GCGTGGCCGCTGGCTCGCTGGGTCTGCCTGACCTGGGCATCTCGAACCTGGACGACGTGCTGACCGACATCCGCCGCATC
ACCGACGTGTGCGACACGCCGCTGCTGGTCGACGTCGACACCGGCTTCGGCGCCTCGGCATTCAACGTGGCCCGCACCAC
GCGCTCGCTGATCAAGTTCGGCGCAGCCGCCATGCATATCGAAGACCAGGTCGGCGCCAAGCGCTGCGGCCACCGTCCGA
ACAAGGAAATCGTCACCAAGGGTGAAATGGTCGACCGCATCCGTGCCGCCGTCGATGCACGTACGGACGAGAACTTCGTC
ATCATGGCCCGCACCGACGCGCTGGCCGTCGAAGGCCTCGACAGCGCGATCGAACGCGCCGTGGCCTGCGTGGAAGCCGG
CGCCGACGCCATCTTCCCCGAGGCGATGACCGACATGGACATGTACCGCAAGTTCGTCGATGCCGTGAAGGTACCCGTGC
TGGCCAACCTGACCGAATTCGGCGCCACGCCGTACTACACGCTGGATGAACTGCGCAACGCCGGCGTTGCCATGGCGCTC
TACCCGCTGTCGGCGTTCCGCGCCATGAACAAGGCGGCCGAGAATGTCTTCCAGGCGATCCGCCGCGAGGGCACCCAGAA
GAACGTGGTCGACACGATGCAGACGCGCGCCGAACTGTACGATCGCATCGGCTACCATGACTTCGAGCAGAAGCTCGACG
CGCTCTTCGCACAAGGCAAGGGCCGCTGA

Upstream 100 bases:

>100_bases
CACCTGCCGCAAGCATCACTTGGCAGCTGGCACACCCCTTGCGCCATAGGGCCACCCTGTCCCGACAGGTTTCAGCATTC
ACGAATAACGGATTCACGCC

Downstream 100 bases:

>100_bases
TCCAGCACCCACCGCCATGACCGCACGCAAACACACCAAGCTTAACCAGGAGACCCAAATGTCCGAAGCCCAACCGCTGG
CCACCCCGAAGCCCAAGAAG

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTFTATELARSAGARFRQALIDESPLQIPGTINANHALLAKRAGYRAIYLSGGGVAAGSLGLPDLGISNLDDVLTDIRRI
TDVCDTPLLVDVDTGFGASAFNVARTTRSLIKFGAAAMHIEDQVGAKRCGHRPNKEIVTKGEMVDRIRAAVDARTDENFV
IMARTDALAVEGLDSAIERAVACVEAGADAIFPEAMTDMDMYRKFVDAVKVPVLANLTEFGATPYYTLDELRNAGVAMAL
YPLSAFRAMNKAAENVFQAIRREGTQKNVVDTMQTRAELYDRIGYHDFEQKLDALFAQGKGR

Sequences:

>Translated_302_residues
MTFTATELARSAGARFRQALIDESPLQIPGTINANHALLAKRAGYRAIYLSGGGVAAGSLGLPDLGISNLDDVLTDIRRI
TDVCDTPLLVDVDTGFGASAFNVARTTRSLIKFGAAAMHIEDQVGAKRCGHRPNKEIVTKGEMVDRIRAAVDARTDENFV
IMARTDALAVEGLDSAIERAVACVEAGADAIFPEAMTDMDMYRKFVDAVKVPVLANLTEFGATPYYTLDELRNAGVAMAL
YPLSAFRAMNKAAENVFQAIRREGTQKNVVDTMQTRAELYDRIGYHDFEQKLDALFAQGKGR
>Mature_301_residues
TFTATELARSAGARFRQALIDESPLQIPGTINANHALLAKRAGYRAIYLSGGGVAAGSLGLPDLGISNLDDVLTDIRRIT
DVCDTPLLVDVDTGFGASAFNVARTTRSLIKFGAAAMHIEDQVGAKRCGHRPNKEIVTKGEMVDRIRAAVDARTDENFVI
MARTDALAVEGLDSAIERAVACVEAGADAIFPEAMTDMDMYRKFVDAVKVPVLANLTEFGATPYYTLDELRNAGVAMALY
PLSAFRAMNKAAENVFQAIRREGTQKNVVDTMQTRAELYDRIGYHDFEQKLDALFAQGKGR

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=291, Percent_Identity=75.9450171821306, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1790445, Length=285, Percent_Identity=24.9122807017544, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32756; Mature: 32624

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTATELARSAGARFRQALIDESPLQIPGTINANHALLAKRAGYRAIYLSGGGVAAGSL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCEEECCC
GLPDLGISNLDDVLTDIRRITDVCDTPLLVDVDTGFGASAFNVARTTRSLIKFGAAAMHI
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
EDQVGAKRCGHRPNKEIVTKGEMVDRIRAAVDARTDENFVIMARTDALAVEGLDSAIERA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHH
VACVEAGADAIFPEAMTDMDMYRKFVDAVKVPVLANLTEFGATPYYTLDELRNAGVAMAL
HHHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCEEEEH
YPLSAFRAMNKAAENVFQAIRREGTQKNVVDTMQTRAELYDRIGYHDFEQKLDALFAQGK
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
GR
CC
>Mature Secondary Structure 
TFTATELARSAGARFRQALIDESPLQIPGTINANHALLAKRAGYRAIYLSGGGVAAGSL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCEEECCC
GLPDLGISNLDDVLTDIRRITDVCDTPLLVDVDTGFGASAFNVARTTRSLIKFGAAAMHI
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
EDQVGAKRCGHRPNKEIVTKGEMVDRIRAAVDARTDENFVIMARTDALAVEGLDSAIERA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHH
VACVEAGADAIFPEAMTDMDMYRKFVDAVKVPVLANLTEFGATPYYTLDELRNAGVAMAL
HHHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCEEEEH
YPLSAFRAMNKAAENVFQAIRREGTQKNVVDTMQTRAELYDRIGYHDFEQKLDALFAQGK
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
GR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]