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The map label for this gene is prpC [H]

Identifier: 94310528

GI number: 94310528

Start: 1708847

End: 1710004

Strand: Reverse

Name: prpC [H]

Synonym: Rmet_1586

Alternate gene names: 94310528

Gene position: 1710004-1708847 (Counterclockwise)

Preceding gene: 94310529

Following gene: 94310527

Centisome position: 43.53

GC content: 63.82

Gene sequence:

>1158_bases
ATGTCCGAAGCCCAACCGCTGGCCACCCCGAAGCCCAAGAAGTCCGTTGCCCTGTCAGGTATCGCCGCCGGCAATACCGC
ACTGTGCACCGTGGGACGCAGCGGCAATGACCTGCACTATCGTGGCTACGACATCCTCGACATCGCCGACACCTGCGAAT
TCGAGGAAATCGCTCACCTTCTGGTCCACGGCAAGCTGCCGACCAAGTCCGAACTGGCCGCCTACAAGGTCAAGCTGAAG
GCACTGCGCGGCCTGCCCGCCAACGTAAAGGCCGCCCTGGAATGGGTGCCGGCCAGCGCCCACCCGATGGACGTGATGCG
CACTGGCGTGTCGGTGCTGGGCACCGTGCTGCCGGAAAAGGACGACCACAACACCCCGGGCGCGCGTGACATCGCCGACC
GCCTGATGGCCAGCCTCGGCTCTATGCTGCTGTACTGGTACCACTACAGCCACAACGGCCGCCGCATCGAAGTGGAAACC
GATGACGACTCGATCGCCGCCCACTTCCTGCACCTGCTGCACGGCGAAGCCCCGTCGAAGCTGTGGGAACGCGCGATGCA
GACTTCGCTGATCCTGTATGCAGAGCACGAGTTCAACGCCTCGACGTTCGCCTGCCGCGTGGTGGCTGGCACGGGCTCCG
ACATGTACTCGGCCATCTGCGGCGGCATCGGCGCGCTGCGTGGTCCGAAGCACGGCGGCGCCAACGAAGTGGCCTTCGAG
ATCCAGAAGCGCTACGACACGCCGGACGAGGCAGAGGCCGACATCCGTCGCCGCGTGGAGAACAAGGAAGTGGTGATCGG
TTTCGGTCACCCCGTGTACACGATCTCCGACCCGCGTAACGTGGTGATCAAGGAAGTGGCGCGCAAGCTGTCGAAGGACG
CCGGTTCGATGAAGATGTTCAACATCGCCGAGCGCCTGGAAACGGTGATGGCCGACGCCAAGAAGATGTTCCCGAACCTG
GACTGGTTCAGCGCGGTGAGCTACCACATGATGGGTGTGCCGACCGCGATGTTCACGCCGCTGTTCGTGATCGCCCGTAC
CTCGGGCTGGGCCGCGCACGTGATCGAGCAACGGGTCGACAACAAGATCATCCGCCCGACCGCCAACTACACCGGCCCGG
AAAACCTGAAGTTCGTGCCGATCGAAAAACGCGCCTGA

Upstream 100 bases:

>100_bases
CAGAAGCTCGACGCGCTCTTCGCACAAGGCAAGGGCCGCTGATCCAGCACCCACCGCCATGACCGCACGCAAACACACCA
AGCTTAACCAGGAGACCCAA

Downstream 100 bases:

>100_bases
TTCGATACCCAGGCACGCAAGATCCGCAAGAAAAACGTGCCGCCGTCCGCGCCCCAGGGCGCGCCGGCCGGCGCGAAACA
GTCCTCACACGACGCCATGA

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSEAQPLATPKPKKSVALSGIAAGNTALCTVGRSGNDLHYRGYDILDIADTCEFEEIAHLLVHGKLPTKSELAAYKVKLK
ALRGLPANVKAALEWVPASAHPMDVMRTGVSVLGTVLPEKDDHNTPGARDIADRLMASLGSMLLYWYHYSHNGRRIEVET
DDDSIAAHFLHLLHGEAPSKLWERAMQTSLILYAEHEFNASTFACRVVAGTGSDMYSAICGGIGALRGPKHGGANEVAFE
IQKRYDTPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNVVIKEVARKLSKDAGSMKMFNIAERLETVMADAKKMFPNL
DWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHVIEQRVDNKIIRPTANYTGPENLKFVPIEKRA

Sequences:

>Translated_385_residues
MSEAQPLATPKPKKSVALSGIAAGNTALCTVGRSGNDLHYRGYDILDIADTCEFEEIAHLLVHGKLPTKSELAAYKVKLK
ALRGLPANVKAALEWVPASAHPMDVMRTGVSVLGTVLPEKDDHNTPGARDIADRLMASLGSMLLYWYHYSHNGRRIEVET
DDDSIAAHFLHLLHGEAPSKLWERAMQTSLILYAEHEFNASTFACRVVAGTGSDMYSAICGGIGALRGPKHGGANEVAFE
IQKRYDTPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNVVIKEVARKLSKDAGSMKMFNIAERLETVMADAKKMFPNL
DWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHVIEQRVDNKIIRPTANYTGPENLKFVPIEKRA
>Mature_384_residues
SEAQPLATPKPKKSVALSGIAAGNTALCTVGRSGNDLHYRGYDILDIADTCEFEEIAHLLVHGKLPTKSELAAYKVKLKA
LRGLPANVKAALEWVPASAHPMDVMRTGVSVLGTVLPEKDDHNTPGARDIADRLMASLGSMLLYWYHYSHNGRRIEVETD
DDSIAAHFLHLLHGEAPSKLWERAMQTSLILYAEHEFNASTFACRVVAGTGSDMYSAICGGIGALRGPKHGGANEVAFEI
QKRYDTPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNVVIKEVARKLSKDAGSMKMFNIAERLETVMADAKKMFPNLD
WFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHVIEQRVDNKIIRPTANYTGPENLKFVPIEKRA

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=374, Percent_Identity=78.3422459893048, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1786939, Length=369, Percent_Identity=32.2493224932249, Blast_Score=178, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17555174, Length=331, Percent_Identity=24.773413897281, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324328, Length=342, Percent_Identity=25.4385964912281, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319850, Length=342, Percent_Identity=24.2690058479532, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24640126, Length=335, Percent_Identity=25.3731343283582, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24640124, Length=335, Percent_Identity=25.3731343283582, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21356863, Length=354, Percent_Identity=24.2937853107345, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 42398; Mature: 42267

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAQPLATPKPKKSVALSGIAAGNTALCTVGRSGNDLHYRGYDILDIADTCEFEEIAHL
CCCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCEECCEEEEEHHHCCCHHHHHHH
LVHGKLPTKSELAAYKVKLKALRGLPANVKAALEWVPASAHPMDVMRTGVSVLGTVLPEK
HHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
DDHNTPGARDIADRLMASLGSMLLYWYHYSHNGRRIEVETDDDSIAAHFLHLLHGEAPSK
CCCCCCCHHHHHHHHHHHHHHHHEEHEEECCCCCEEEEECCCHHHHHHHHHHHHCCCHHH
LWERAMQTSLILYAEHEFNASTFACRVVAGTGSDMYSAICGGIGALRGPKHGGANEVAFE
HHHHHHHCEEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
IQKRYDTPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNVVIKEVARKLSKDAGSMKMF
HHHHCCCCCHHHHHHHHHCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHH
NIAERLETVMADAKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHVIEQRVD
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHEEEEEECCCHHHHHHHHHHC
NKIIRPTANYTGPENLKFVPIEKRA
CCEECCCCCCCCCCCCEEEECCCCC
>Mature Secondary Structure 
SEAQPLATPKPKKSVALSGIAAGNTALCTVGRSGNDLHYRGYDILDIADTCEFEEIAHL
CCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCEECCEEEEEHHHCCCHHHHHHH
LVHGKLPTKSELAAYKVKLKALRGLPANVKAALEWVPASAHPMDVMRTGVSVLGTVLPEK
HHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
DDHNTPGARDIADRLMASLGSMLLYWYHYSHNGRRIEVETDDDSIAAHFLHLLHGEAPSK
CCCCCCCHHHHHHHHHHHHHHHHEEHEEECCCCCEEEEECCCHHHHHHHHHHHHCCCHHH
LWERAMQTSLILYAEHEFNASTFACRVVAGTGSDMYSAICGGIGALRGPKHGGANEVAFE
HHHHHHHCEEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
IQKRYDTPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNVVIKEVARKLSKDAGSMKMF
HHHHCCCCCHHHHHHHHHCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHH
NIAERLETVMADAKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHVIEQRVD
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHEEEEEECCCHHHHHHHHHHC
NKIIRPTANYTGPENLKFVPIEKRA
CCEECCCCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]