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The map label for this gene is pntB [H]

Identifier: 94310474

GI number: 94310474

Start: 1652539

End: 1653912

Strand: Direct

Name: pntB [H]

Synonym: Rmet_1532

Alternate gene names: 94310474

Gene position: 1652539-1653912 (Clockwise)

Preceding gene: 94310469

Following gene: 94310475

Centisome position: 42.07

GC content: 64.34

Gene sequence:

>1374_bases
ATGAGCGACGTCATCCAGTTGGCGTACTTCGCAGTGGCCGTGGTCTTCATTCTCGGCCTGAAGGCGATGAGCTCGCCGGT
CTCCGCCCGCAAGGGCATCGTGTGGGCCGGCTACGCGATGGTCGCGGCCACCCTTATCACACTGCTTAAACCGGGCATGG
AAAATTACGGCCTGATGATCACCGCCATTGTGCTCGGCGGTGCCGTCGCGTGGTGGTCCGGCAAGGTCGTCAAGATGACT
GACATGCCGCAGATGGTCGCGATCTACAACGGCATGGGCAGCGGCGCGGCAGCGGCGATCGCAGCGCTCGAGTTCGCGCG
CGGCAGCATGCATGGCGGCTTCGCTGCGACGCTTGCGGTGCTTGGTGCGCTGATCGGCTCGGTGGCGTTCTCGGGGTCAT
GCATCGCCTTCGCAAAGCTGCAGGGCATCATGACCAAGGCGTGGCGGCTGCCGGCGCAGAACGTGGTGAATGTTGCGCTC
GCCGGGATTGCCCTGGTGCTCGGCGGCGCGATCATCTTTGGCAGCACTCCGGCGTCAGAAATGGTGATCGCATTTTTCGT
GGTTGCGCTTGCCCTCGGCGCAATCCTCACCAGCCCGATCGGCGGCGCCGACATGCCGGTTGTGATTTCGCTCCTGAACG
CATTCACAGGCCTCGCGGTCGGTTTCGAGGGTTACGTGCTCGGCAACCCGGCGCTGATCGTCGCGGGCATCGTCGTCGGT
GCGTCCGGTACGCTGCTGACGCAGTTGATGGCGAAGGCGATGAACCGCCCGATCAAGAACATCATCTTCACGCCGATCAC
CGGCGCGGCGGCGGGTGGTGGCGAGGCGATCGGGGGCTCGATGAAGGAGCTCTCCGCACTGGACGGCGCTTCGGTGATGC
GCTACGCGTCCAAGGTCATCATCGTGCCGGGCTACGGCATGGCGGTCGCCGGTGCGCAGCACAAGGTGTGGGAGATGGCG
CAGTTGCTGGAAGAAGGTGGCGTCGAGGTCGTGTTCGCGATCCATCCGGTCGCGGGCCGCATGCCGGGTCACATGAACGT
GCTGCTCGCGGAGGCCGGCGTGCCCTACGACAAGATCTTCGACCTCGAGGAGATCAACGCCGACTTCCCGCAGGCGGATG
TGGCGCTCGTGATCGGCGCGAATGACGTCGTGAACCCGGTCGCGCGCACCGACAAGTCGAGCCCGATCTACGGCATGCCG
ATCCTCAACGTCGACATGGCGCAGAACGTCATCGTCGTGAAACGCGGGCAGGGCGCGGGCTACTCGGGCATCGAGAATGC
GCTGTTCTTCAAGGACAACTGCCGCATGCTGTACGGCAGTGCGCAGCAGATCATCGGCGAAGTCATCACCCATGTGAAGG
CGCTGGAGGTCTAA

Upstream 100 bases:

>100_bases
CAGTGTTCCTCGGCGCGGCGAACGCTGCCGGCGGTTACGTCGTGACCGAGCGCATGCTCGCGATGTTCAAGCCGGGCGCC
AACAAGGCGGGAGGTGCGCA

Downstream 100 bases:

>100_bases
TTTGATACAGTCAAATTAAGATGAAGTAGAATTTCTTCATAACCTAGTCCAGGGGAGCGGAATGGAAGCACTGCCGAACG
ATTGGGTGGCCCTGGCCGCG

Product: NAD(P) transhydrogenase subunit beta

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSDVIQLAYFAVAVVFILGLKAMSSPVSARKGIVWAGYAMVAATLITLLKPGMENYGLMITAIVLGGAVAWWSGKVVKMT
DMPQMVAIYNGMGSGAAAAIAALEFARGSMHGGFAATLAVLGALIGSVAFSGSCIAFAKLQGIMTKAWRLPAQNVVNVAL
AGIALVLGGAIIFGSTPASEMVIAFFVVALALGAILTSPIGGADMPVVISLLNAFTGLAVGFEGYVLGNPALIVAGIVVG
ASGTLLTQLMAKAMNRPIKNIIFTPITGAAAGGGEAIGGSMKELSALDGASVMRYASKVIIVPGYGMAVAGAQHKVWEMA
QLLEEGGVEVVFAIHPVAGRMPGHMNVLLAEAGVPYDKIFDLEEINADFPQADVALVIGANDVVNPVARTDKSSPIYGMP
ILNVDMAQNVIVVKRGQGAGYSGIENALFFKDNCRMLYGSAQQIIGEVITHVKALEV

Sequences:

>Translated_457_residues
MSDVIQLAYFAVAVVFILGLKAMSSPVSARKGIVWAGYAMVAATLITLLKPGMENYGLMITAIVLGGAVAWWSGKVVKMT
DMPQMVAIYNGMGSGAAAAIAALEFARGSMHGGFAATLAVLGALIGSVAFSGSCIAFAKLQGIMTKAWRLPAQNVVNVAL
AGIALVLGGAIIFGSTPASEMVIAFFVVALALGAILTSPIGGADMPVVISLLNAFTGLAVGFEGYVLGNPALIVAGIVVG
ASGTLLTQLMAKAMNRPIKNIIFTPITGAAAGGGEAIGGSMKELSALDGASVMRYASKVIIVPGYGMAVAGAQHKVWEMA
QLLEEGGVEVVFAIHPVAGRMPGHMNVLLAEAGVPYDKIFDLEEINADFPQADVALVIGANDVVNPVARTDKSSPIYGMP
ILNVDMAQNVIVVKRGQGAGYSGIENALFFKDNCRMLYGSAQQIIGEVITHVKALEV
>Mature_456_residues
SDVIQLAYFAVAVVFILGLKAMSSPVSARKGIVWAGYAMVAATLITLLKPGMENYGLMITAIVLGGAVAWWSGKVVKMTD
MPQMVAIYNGMGSGAAAAIAALEFARGSMHGGFAATLAVLGALIGSVAFSGSCIAFAKLQGIMTKAWRLPAQNVVNVALA
GIALVLGGAIIFGSTPASEMVIAFFVVALALGAILTSPIGGADMPVVISLLNAFTGLAVGFEGYVLGNPALIVAGIVVGA
SGTLLTQLMAKAMNRPIKNIIFTPITGAAAGGGEAIGGSMKELSALDGASVMRYASKVIIVPGYGMAVAGAQHKVWEMAQ
LLEEGGVEVVFAIHPVAGRMPGHMNVLLAEAGVPYDKIFDLEEINADFPQADVALVIGANDVVNPVARTDKSSPIYGMPI
LNVDMAQNVIVVKRGQGAGYSGIENALFFKDNCRMLYGSAQQIIGEVITHVKALEV

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=466, Percent_Identity=38.6266094420601, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI122939153, Length=466, Percent_Identity=38.6266094420601, Blast_Score=296, Evalue=3e-80,
Organism=Escherichia coli, GI1787886, Length=452, Percent_Identity=42.0353982300885, Blast_Score=321, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI17550456, Length=462, Percent_Identity=38.7445887445887, Blast_Score=298, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 47016; Mature: 46885

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVIQLAYFAVAVVFILGLKAMSSPVSARKGIVWAGYAMVAATLITLLKPGMENYGLMI
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEHHHHHHHHHHHHHCCCCCCCCHHH
TAIVLGGAVAWWSGKVVKMTDMPQMVAIYNGMGSGAAAAIAALEFARGSMHGGFAATLAV
HHHHHHCCCEECCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
LGALIGSVAFSGSCIAFAKLQGIMTKAWRLPAQNVVNVALAGIALVLGGAIIFGSTPASE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHH
MVIAFFVVALALGAILTSPIGGADMPVVISLLNAFTGLAVGFEGYVLGNPALIVAGIVVG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHC
ASGTLLTQLMAKAMNRPIKNIIFTPITGAAAGGGEAIGGSMKELSALDGASVMRYASKVI
CCHHHHHHHHHHHHCCCHHHHEECCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHCCCEE
IVPGYGMAVAGAQHKVWEMAQLLEEGGVEVVFAIHPVAGRMPGHMNVLLAEAGVPYDKIF
EECCCCCEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCCHHHHC
DLEEINADFPQADVALVIGANDVVNPVARTDKSSPIYGMPILNVDMAQNVIVVKRGQGAG
CHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCCEECCCEEECCCCCCEEEEECCCCCC
YSGIENALFFKDNCRMLYGSAQQIIGEVITHVKALEV
CCCCCCCEEEECCCEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDVIQLAYFAVAVVFILGLKAMSSPVSARKGIVWAGYAMVAATLITLLKPGMENYGLMI
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEHHHHHHHHHHHHHCCCCCCCCHHH
TAIVLGGAVAWWSGKVVKMTDMPQMVAIYNGMGSGAAAAIAALEFARGSMHGGFAATLAV
HHHHHHCCCEECCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
LGALIGSVAFSGSCIAFAKLQGIMTKAWRLPAQNVVNVALAGIALVLGGAIIFGSTPASE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHH
MVIAFFVVALALGAILTSPIGGADMPVVISLLNAFTGLAVGFEGYVLGNPALIVAGIVVG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHC
ASGTLLTQLMAKAMNRPIKNIIFTPITGAAAGGGEAIGGSMKELSALDGASVMRYASKVI
CCHHHHHHHHHHHHCCCHHHHEECCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHCCCEE
IVPGYGMAVAGAQHKVWEMAQLLEEGGVEVVFAIHPVAGRMPGHMNVLLAEAGVPYDKIF
EECCCCCEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCCHHHHC
DLEEINADFPQADVALVIGANDVVNPVARTDKSSPIYGMPILNVDMAQNVIVVKRGQGAG
CHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCCEECCCEEECCCCCCEEEEECCCCCC
YSGIENALFFKDNCRMLYGSAQQIIGEVITHVKALEV
CCCCCCCEEEECCCEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]