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The map label for this gene is pykA2 [H]

Identifier: 94310461

GI number: 94310461

Start: 1640960

End: 1642378

Strand: Direct

Name: pykA2 [H]

Synonym: Rmet_1519

Alternate gene names: 94310461

Gene position: 1640960-1642378 (Clockwise)

Preceding gene: 94310460

Following gene: 94310462

Centisome position: 41.78

GC content: 69.91

Gene sequence:

>1419_bases
ATGCGCCGCAACCGCAACGCCAAGATCGTCGCCACGCTCGGCCCGTCCAGTTCCGACAAGGACACCATCCGCCGGCTGTT
CGCCGCCGGTGTCGATGTCTTTCGCCTGAACTTCAGTCACGGCGACGAGGCGGCGCATCGGGCCAGCTTCGCCATCCTGC
GTGAACTCGAGCGCGAGGCCGGCCGCCCGATTGGCATCCTGGCCGACCTGCAGGGTCCGAAGCTGCGCGTGGGCACTTTC
GCCGCGGGTTCGGTGATGCTGGACGAATCGCAGCCGTTCCGCCTCGAGCTCGACACGGCGCCGGGTGACGCGCGCCGCGT
CGGCCTGCCGCATCCGGAGATCTTCGCGGCGCTGGCGCCGGGCGCCGAACTGCTGCTCGACGACGGCAAGCTGCGGCTGA
TCGTCGAACGCTGCGGCGCCGACTTCGCCGATACGCGCGTGGTGGCCGGCGGCAAGCTGTCCGATCGCAAGGGCGTCAAC
GTGCCCGGCGTGCTGCTACCCATCAGCGCACTGACCGCCAAGGACCGGCGCGATCTGGCGCTGGCGCTGGACCTCGGTGC
GGACTGGATCGCCCTGTCGTTCGTGCAGCGGCCCGAAGACGTCGTCGAGGCGCGAAAGCTGATCGGCGAACGCGCGGCCA
TCGTTGCGAAGCTCGAGAAGCCGTCGGCGGTCGAACAACTGGAAGCCATCGCCGAATGCGCCGACGCCGTGATGGTGGCG
CGCGGTGACCTGGGCGTGGAGTTGCCGGCCGAGCGCGTGCCCGGGATACAGAAACGCATCCTCCGCACCTGCCGCCGGCT
CGGACGGCCGGTGATCGTGGCCACGCAGATGCTTGAATCGATGGTGACGAGCCCGGTGCCTACGCGCGCCGAGGCCTCGG
ACGTGGCCACTGCGATCTACGACGGCGCCGACGCGGTCATGTTGTCCGCCGAATCCGCTGCCGGCCGCTATCCGGTCGAG
GCGGTGGACATGATGAGCCGCCTGATCACCGAAGTGGAGGGCGATCCGCAGTACCTGCAGCTGATGAACGCGTCGCGCCC
GAGCGCGCCGGCTGGCGCGGGTGCCGCCGACGCCGTGTGCTGCGCCGTGCGCCATGCCGTCGCGCTGATGCATGCGAGCG
CCATCGTCTGCTACACGAGCTCGGGCCACACCAGCCTGCGTGTCGCCCGTGAGCGTCCCGAGGCGCCGGTACTGAGCCTG
ACGCCGCATTTGCGCGTCGCGCGCCGCATGGCGCTCGCCTGGGGCATCCACTCGGTGCACATCGAGGACGTGGCCGATGT
CGATCAGATGACCGCGAAAGCCTGCGCGGTCGCCTGCCAGGAAGGATTCGCCCAGGGCGGGCAGACCCTCGTCGTCATTG
CCGGCGTGCCGTTCGGCACGCCCGGCACGACGAATCTCATGCGCATCGCGACGGTGTGA

Upstream 100 bases:

>100_bases
CGCGCTTCGAGGCCTTCGGCTGCGCCGGGCAGGGGGCCCGCATCAAGCCGCTCCCGCTCGAAGTCATGGCGGCACGCTAC
GCGTGAAGGCCCGCCACACC

Downstream 100 bases:

>100_bases
ATCCAGTCCCTCTTAACAGACCAAAGAGCCCCCCGATGGACCCGACCCCCCTCCCGCCGCACCCAGTTCGCGGCCAACGC
CGACGACTGCTGGTGGCCGG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MRRNRNAKIVATLGPSSSDKDTIRRLFAAGVDVFRLNFSHGDEAAHRASFAILRELEREAGRPIGILADLQGPKLRVGTF
AAGSVMLDESQPFRLELDTAPGDARRVGLPHPEIFAALAPGAELLLDDGKLRLIVERCGADFADTRVVAGGKLSDRKGVN
VPGVLLPISALTAKDRRDLALALDLGADWIALSFVQRPEDVVEARKLIGERAAIVAKLEKPSAVEQLEAIAECADAVMVA
RGDLGVELPAERVPGIQKRILRTCRRLGRPVIVATQMLESMVTSPVPTRAEASDVATAIYDGADAVMLSAESAAGRYPVE
AVDMMSRLITEVEGDPQYLQLMNASRPSAPAGAGAADAVCCAVRHAVALMHASAIVCYTSSGHTSLRVARERPEAPVLSL
TPHLRVARRMALAWGIHSVHIEDVADVDQMTAKACAVACQEGFAQGGQTLVVIAGVPFGTPGTTNLMRIATV

Sequences:

>Translated_472_residues
MRRNRNAKIVATLGPSSSDKDTIRRLFAAGVDVFRLNFSHGDEAAHRASFAILRELEREAGRPIGILADLQGPKLRVGTF
AAGSVMLDESQPFRLELDTAPGDARRVGLPHPEIFAALAPGAELLLDDGKLRLIVERCGADFADTRVVAGGKLSDRKGVN
VPGVLLPISALTAKDRRDLALALDLGADWIALSFVQRPEDVVEARKLIGERAAIVAKLEKPSAVEQLEAIAECADAVMVA
RGDLGVELPAERVPGIQKRILRTCRRLGRPVIVATQMLESMVTSPVPTRAEASDVATAIYDGADAVMLSAESAAGRYPVE
AVDMMSRLITEVEGDPQYLQLMNASRPSAPAGAGAADAVCCAVRHAVALMHASAIVCYTSSGHTSLRVARERPEAPVLSL
TPHLRVARRMALAWGIHSVHIEDVADVDQMTAKACAVACQEGFAQGGQTLVVIAGVPFGTPGTTNLMRIATV
>Mature_472_residues
MRRNRNAKIVATLGPSSSDKDTIRRLFAAGVDVFRLNFSHGDEAAHRASFAILRELEREAGRPIGILADLQGPKLRVGTF
AAGSVMLDESQPFRLELDTAPGDARRVGLPHPEIFAALAPGAELLLDDGKLRLIVERCGADFADTRVVAGGKLSDRKGVN
VPGVLLPISALTAKDRRDLALALDLGADWIALSFVQRPEDVVEARKLIGERAAIVAKLEKPSAVEQLEAIAECADAVMVA
RGDLGVELPAERVPGIQKRILRTCRRLGRPVIVATQMLESMVTSPVPTRAEASDVATAIYDGADAVMLSAESAAGRYPVE
AVDMMSRLITEVEGDPQYLQLMNASRPSAPAGAGAADAVCCAVRHAVALMHASAIVCYTSSGHTSLRVARERPEAPVLSL
TPHLRVARRMALAWGIHSVHIEDVADVDQMTAKACAVACQEGFAQGGQTLVVIAGVPFGTPGTTNLMRIATV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=36.8098159509202, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=35.7873210633947, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=35.7873210633947, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=35.5828220858896, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=35.5828220858896, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=35.191637630662, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=35.191637630662, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=31.4893617021277, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=31.4893617021277, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=30.8411214953271, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=39.9168399168399, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1787965, Length=468, Percent_Identity=35.2564102564103, Blast_Score=284, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=35.7289527720739, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=36.7768595041322, Blast_Score=274, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=36.3636363636364, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=36.7768595041322, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=36.3636363636364, Blast_Score=273, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=32.9218106995885, Blast_Score=243, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=484, Percent_Identity=33.2644628099174, Blast_Score=243, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24648964, Length=492, Percent_Identity=36.3821138211382, Blast_Score=288, Evalue=7e-78,
Organism=Drosophila melanogaster, GI28571814, Length=492, Percent_Identity=36.3821138211382, Blast_Score=288, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24581235, Length=483, Percent_Identity=31.4699792960663, Blast_Score=234, Evalue=7e-62,
Organism=Drosophila melanogaster, GI24648966, Length=412, Percent_Identity=35.4368932038835, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24646914, Length=261, Percent_Identity=38.3141762452107, Blast_Score=161, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50108; Mature: 50108

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRNRNAKIVATLGPSSSDKDTIRRLFAAGVDVFRLNFSHGDEAAHRASFAILRELEREA
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
GRPIGILADLQGPKLRVGTFAAGSVMLDESQPFRLELDTAPGDARRVGLPHPEIFAALAP
CCCEEEEEECCCCEEEEEEEEECEEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCC
GAELLLDDGKLRLIVERCGADFADTRVVAGGKLSDRKGVNVPGVLLPISALTAKDRRDLA
CCEEEECCCCEEEEEEHHCCCHHHCEEEECCCCCCCCCCCCCCCEEEHHHHCCCCCCCEE
LALDLGADWIALSFVQRPEDVVEARKLIGERAAIVAKLEKPSAVEQLEAIAECADAVMVA
EEEECCCCHHHHHHHHCCHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHHHHHHHHEEE
RGDLGVELPAERVPGIQKRILRTCRRLGRPVIVATQMLESMVTSPVPTRAEASDVATAIY
CCCCCCCCCHHHCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DGADAVMLSAESAAGRYPVEAVDMMSRLITEVEGDPQYLQLMNASRPSAPAGAGAADAVC
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHH
CAVRHAVALMHASAIVCYTSSGHTSLRVARERPEAPVLSLTPHLRVARRMALAWGIHSVH
HHHHHHHHHHHHCEEEEEECCCCCEEEEECCCCCCCEEECCHHHHHHHHHHHHHCCEEEE
IEDVADVDQMTAKACAVACQEGFAQGGQTLVVIAGVPFGTPGTTNLMRIATV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEECC
>Mature Secondary Structure
MRRNRNAKIVATLGPSSSDKDTIRRLFAAGVDVFRLNFSHGDEAAHRASFAILRELEREA
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
GRPIGILADLQGPKLRVGTFAAGSVMLDESQPFRLELDTAPGDARRVGLPHPEIFAALAP
CCCEEEEEECCCCEEEEEEEEECEEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCC
GAELLLDDGKLRLIVERCGADFADTRVVAGGKLSDRKGVNVPGVLLPISALTAKDRRDLA
CCEEEECCCCEEEEEEHHCCCHHHCEEEECCCCCCCCCCCCCCCEEEHHHHCCCCCCCEE
LALDLGADWIALSFVQRPEDVVEARKLIGERAAIVAKLEKPSAVEQLEAIAECADAVMVA
EEEECCCCHHHHHHHHCCHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHHHHHHHHEEE
RGDLGVELPAERVPGIQKRILRTCRRLGRPVIVATQMLESMVTSPVPTRAEASDVATAIY
CCCCCCCCCHHHCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DGADAVMLSAESAAGRYPVEAVDMMSRLITEVEGDPQYLQLMNASRPSAPAGAGAADAVC
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHH
CAVRHAVALMHASAIVCYTSSGHTSLRVARERPEAPVLSLTPHLRVARRMALAWGIHSVH
HHHHHHHHHHHHCEEEEEECCCCCEEEEECCCCCCCEEECCHHHHHHHHHHHHHCCEEEE
IEDVADVDQMTAKACAVACQEGFAQGGQTLVVIAGVPFGTPGTTNLMRIATV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA