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The map label for this gene is dmf [H]

Identifier: 94310371

GI number: 94310371

Start: 1547523

End: 1548056

Strand: Direct

Name: dmf [H]

Synonym: Rmet_1429

Alternate gene names: 94310371

Gene position: 1547523-1548056 (Clockwise)

Preceding gene: 94310370

Following gene: 94310373

Centisome position: 39.4

GC content: 62.36

Gene sequence:

>534_bases
ATGATCCGGGACATTCTGAAGATGGGCGACGCACGCCTGCTGCAACAGGCGCGCCCCGTGACGCAGTTCAATACGCCTGA
GCTGCGCCTGCTGATCGACGACATGTTCGACACGATGGAACATGCCAACGGCGCCGGCCTTGCCGCGCCGCAGATCGGCG
TGGACCTGCAGGTGGTGATTTTCGGTTTCGATCGCAACCCGCGCTATCCCGACGCACCGACCGTGCCGAAGACCGTGCTG
ATCAATCCATCCCTGGAGCCGCTGTCCGACGAGATGGACGATGGATGGGAAGGATGCCTCTCGGTGCCGGGCCTGCGCGG
CGTGGTGCCGCGCTACACGCGGCTGAAATACACGGGCTTCGACATGATGGGCAACAGGATCGAGCGCGTGGCCGAGGACT
TCCATGCGCGCGTGGTGCAGCACGAGTGCGATCACCTGATCGGCGTGCTGTATCCGATGCGGATCAAGGACTTCACGCGC
TTCGGTTTTACCGAGATCCTGTTCCCCGAACTACCACCCAATTACGACGACTGA

Upstream 100 bases:

>100_bases
AGGCGCAGACACTGGGCGTGGCGGTGCTGGACGAGGAAGGGATGTTGAAGTTGCTGGCCGAGGTTGGCGCAGCCTGATAC
GGAGAGAGGGAGAACGCGCA

Downstream 100 bases:

>100_bases
GGCGAGGCGCACGCCGTGATTGCCGGCTGCCCATGCCGGTTGTGATAGACCAGCGCAGCGGCAACCAGGATCGTCATCAG
GCACAACTCGGCGGCGAGGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MIRDILKMGDARLLQQARPVTQFNTPELRLLIDDMFDTMEHANGAGLAAPQIGVDLQVVIFGFDRNPRYPDAPTVPKTVL
INPSLEPLSDEMDDGWEGCLSVPGLRGVVPRYTRLKYTGFDMMGNRIERVAEDFHARVVQHECDHLIGVLYPMRIKDFTR
FGFTEILFPELPPNYDD

Sequences:

>Translated_177_residues
MIRDILKMGDARLLQQARPVTQFNTPELRLLIDDMFDTMEHANGAGLAAPQIGVDLQVVIFGFDRNPRYPDAPTVPKTVL
INPSLEPLSDEMDDGWEGCLSVPGLRGVVPRYTRLKYTGFDMMGNRIERVAEDFHARVVQHECDHLIGVLYPMRIKDFTR
FGFTEILFPELPPNYDD
>Mature_177_residues
MIRDILKMGDARLLQQARPVTQFNTPELRLLIDDMFDTMEHANGAGLAAPQIGVDLQVVIFGFDRNPRYPDAPTVPKTVL
INPSLEPLSDEMDDGWEGCLSVPGLRGVVPRYTRLKYTGFDMMGNRIERVAEDFHARVVQHECDHLIGVLYPMRIKDFTR
FGFTEILFPELPPNYDD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=31.4285714285714, Blast_Score=80, Evalue=8e-16,
Organism=Escherichia coli, GI1789682, Length=147, Percent_Identity=36.734693877551, Blast_Score=90, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24645728, Length=170, Percent_Identity=32.3529411764706, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=35.2564102564103, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20163; Mature: 20163

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRDILKMGDARLLQQARPVTQFNTPELRLLIDDMFDTMEHANGAGLAAPQIGVDLQVVI
CCHHHHHHCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
FGFDRNPRYPDAPTVPKTVLINPSLEPLSDEMDDGWEGCLSVPGLRGVVPRYTRLKYTGF
EECCCCCCCCCCCCCCCEEEECCCCCCCCHHHCCHHHHHHCCCCCCCCCCCCCEEEECCH
DMMGNRIERVAEDFHARVVQHECDHLIGVLYPMRIKDFTRFGFTEILFPELPPNYDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIRDILKMGDARLLQQARPVTQFNTPELRLLIDDMFDTMEHANGAGLAAPQIGVDLQVVI
CCHHHHHHCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
FGFDRNPRYPDAPTVPKTVLINPSLEPLSDEMDDGWEGCLSVPGLRGVVPRYTRLKYTGF
EECCCCCCCCCCCCCCCEEEECCCCCCCCHHHCCHHHHHHCCCCCCCCCCCCCEEEECCH
DMMGNRIERVAEDFHARVVQHECDHLIGVLYPMRIKDFTRFGFTEILFPELPPNYDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]