Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 94310370

GI number: 94310370

Start: 1545334

End: 1547499

Strand: Direct

Name: ligA

Synonym: Rmet_1428

Alternate gene names: 94310370

Gene position: 1545334-1547499 (Clockwise)

Preceding gene: 94310369

Following gene: 94310371

Centisome position: 39.34

GC content: 65.88

Gene sequence:

>2166_bases
ATGACCGCCAAGAAACAAGGCGCGCAGGCTTCGGCCTCCGCGCCGTCCGGCGATTCCCCCGCCGAGCGCGCGCAATGGTT
GCGCGAAGAACTGGAGCGCTACAGCTACCAGTATTACGTGCTCGATGCGCCGACGATTCCCGACGCCGACTACGATGCGC
TGTTCATCGAGTTGCAGGCGCTGGAAGCCGAACATCCTGAGCTGCTGACACCCGATTCCCCCACGCAGCGCGTGGGTGGT
GCGCCATTGTCGGCGTTCGACTCGGTGCGCCACCGCGTGCCGATGCTGTCGCTCAACAATGGCTTCGAGGACGAGGACGT
CATCAACTTCGACCGGCGCTGCGCGCAGGGCCTCGGCCGTGCCACGGCCGCAGCACCCGCAGCATTCGCGACACCCGCCG
CGCCGGCTGACGATCTGTTCAGCGCGGCCGATGCCGCCGAGGCAGCCGCAAACACCGCCGGGAATGCCGTCGAGTACGCC
TGCGAGCTGAAGTTCGACGGGCTCGCGATGTCGCTGCGATACGAGAACGGAACGCTCGTGCAGGCCGCCACGCGCGGCGA
CGGCGAGACGGGCGAAGACGTGACCGCGAACGTGCGCACGATCAAGGCGATTCCGCTCAAGCTGCGCGGCACGGCCCCGT
CCGTGCTGGAGGTGCGCGGAGAGGTGTTCATGTATCGCGCCGATTTCGACAAGCTCAACGCGCGCCAGGCCGAATCGGGC
GAGAAGACATTCGTCAATCCGCGCAACGCGGCGGCGGGCAGCCTTCGCCAGCTTGATCCGCGTATCACGGCCAAGCGCCC
GCTTTCGTTCTTTGCCTATGGCGTGGGCGAGATGGAAGGCTTCGACCGTCCGGGCACGCACAGCGCAATGCTTGACGGCT
TTGCCAAGCTCGGTTTGCCTGTTTGCGACGAGCGACGTGTGGTCCGGGGCGCCCAGGGCCTGCTGTCATTCCATCGCGAG
GTTGGCGAGCGTCGTGACCAGTTGCCGTACGACATCGACGGCGTGGTCTACAAAGTCAATGCGATTGCCGAACAGGAACA
GCTCGGCTTTGTCTCGCGCGCGCCGCGTTTCGCGCTGGCGCACAAGTTTCCTGCACAGGAGATGACCACTACGGTCGAGG
ACATCGAGGTCCAGGTCGGGCGCACCGGTGCGATCACGCCGGTGGCGCGACTGGCGCCAGTGTTTGTTGGCGGGGTCACC
GTGACCAATGCCACGCTGCACAACGAGGACGAGATCCGGCGCAAGGACGTGCATATCGGCGACACGGTGATCGTGCGCCG
CGCGGGTGACGTGATTCCGGAGGTGGTTGCCGTGGTGCTGGAGCGCCGCCCGGCGGATGCCCGTGCGTTCGTGATGCCGA
CTGAATGCCCGGTCTGCGGATCGCACGTGGAAAGGCTCGAAGGCGAGGCGATTGCACGCTGCACGGGCGGGCTGATCTGC
GCTGCGCAACGCAAGCAGGCGCTGCTGCATTTCGCGCAGCGTCGGGCAATGGATATCGAAGGGCTGGGCGACAAGGTGGT
GGAGCAACTGGTCGACCTGGGCATCGTGCGCACGCCTGCCGATCTCTATAAGCTCGGCGTGGCGAAGCTCGCGGCGCTGG
ACCGGATGGCCGACAAGTCGGCATCGAACCTGGTGGCGGCGATCGAGCAGTCTCGCGAGACCACGATGAACCGCTTTATC
TTCGCGCTCGGCATCCGTCATGTAGGAGAGGCCACCGCCAAGGATTTGGCCAAGCACTTCGGCAAGCTGGACAACCTGCT
GGCCGCGGACGAGGCCGCCTTGCTCGAAGTCAACGACGTGGGCCCCGTGGTGGCTCAGTCGATCGCCAACTTCATTGCCG
AACCGCACAATGTCGAAGTTATCGAACAGTTGCGGGCGGCGGGTGTGCACTGGGCCGAGACAGAGCCGACTGCGCGTGCG
CCGCTGCCCCTGGCTGGCAAGACGTTCGTGCTGACCGGCACGCTGCCGACGATGTCCCGCGAGGACGCCAAGGAGAAGCT
GGAAGCCGCTGGCGCCAAGGTGGCGGGTTCGGTGTCGAAGAAGACCGACTACGTAGTGGCCGGGGCCGAGGCGGGCAGCA
AGCTCGAGAAGGCGCAGACACTGGGCGTGGCGGTGCTGGACGAGGAAGGGATGTTGAAGTTGCTGGCCGAGGTTGGCGCA
GCCTGA

Upstream 100 bases:

>100_bases
TCGGCCAACTCGAGAAGTTATACGAGAAGCTGGAATCGCGCGGAATGCCCGCCGGATCGCCTGTTGCCGTGCGCTTGTTC
AGCCAGTAACGGAGCCAGTC

Downstream 100 bases:

>100_bases
TACGGAGAGAGGGAGAACGCGCAATGATCCGGGACATTCTGAAGATGGGCGACGCACGCCTGCTGCAACAGGCGCGCCCC
GTGACGCAGTTCAATACGCC

Product: DNA ligase, NAD(+)-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MTAKKQGAQASASAPSGDSPAERAQWLREELERYSYQYYVLDAPTIPDADYDALFIELQALEAEHPELLTPDSPTQRVGG
APLSAFDSVRHRVPMLSLNNGFEDEDVINFDRRCAQGLGRATAAAPAAFATPAAPADDLFSAADAAEAAANTAGNAVEYA
CELKFDGLAMSLRYENGTLVQAATRGDGETGEDVTANVRTIKAIPLKLRGTAPSVLEVRGEVFMYRADFDKLNARQAESG
EKTFVNPRNAAAGSLRQLDPRITAKRPLSFFAYGVGEMEGFDRPGTHSAMLDGFAKLGLPVCDERRVVRGAQGLLSFHRE
VGERRDQLPYDIDGVVYKVNAIAEQEQLGFVSRAPRFALAHKFPAQEMTTTVEDIEVQVGRTGAITPVARLAPVFVGGVT
VTNATLHNEDEIRRKDVHIGDTVIVRRAGDVIPEVVAVVLERRPADARAFVMPTECPVCGSHVERLEGEAIARCTGGLIC
AAQRKQALLHFAQRRAMDIEGLGDKVVEQLVDLGIVRTPADLYKLGVAKLAALDRMADKSASNLVAAIEQSRETTMNRFI
FALGIRHVGEATAKDLAKHFGKLDNLLAADEAALLEVNDVGPVVAQSIANFIAEPHNVEVIEQLRAAGVHWAETEPTARA
PLPLAGKTFVLTGTLPTMSREDAKEKLEAAGAKVAGSVSKKTDYVVAGAEAGSKLEKAQTLGVAVLDEEGMLKLLAEVGA
A

Sequences:

>Translated_721_residues
MTAKKQGAQASASAPSGDSPAERAQWLREELERYSYQYYVLDAPTIPDADYDALFIELQALEAEHPELLTPDSPTQRVGG
APLSAFDSVRHRVPMLSLNNGFEDEDVINFDRRCAQGLGRATAAAPAAFATPAAPADDLFSAADAAEAAANTAGNAVEYA
CELKFDGLAMSLRYENGTLVQAATRGDGETGEDVTANVRTIKAIPLKLRGTAPSVLEVRGEVFMYRADFDKLNARQAESG
EKTFVNPRNAAAGSLRQLDPRITAKRPLSFFAYGVGEMEGFDRPGTHSAMLDGFAKLGLPVCDERRVVRGAQGLLSFHRE
VGERRDQLPYDIDGVVYKVNAIAEQEQLGFVSRAPRFALAHKFPAQEMTTTVEDIEVQVGRTGAITPVARLAPVFVGGVT
VTNATLHNEDEIRRKDVHIGDTVIVRRAGDVIPEVVAVVLERRPADARAFVMPTECPVCGSHVERLEGEAIARCTGGLIC
AAQRKQALLHFAQRRAMDIEGLGDKVVEQLVDLGIVRTPADLYKLGVAKLAALDRMADKSASNLVAAIEQSRETTMNRFI
FALGIRHVGEATAKDLAKHFGKLDNLLAADEAALLEVNDVGPVVAQSIANFIAEPHNVEVIEQLRAAGVHWAETEPTARA
PLPLAGKTFVLTGTLPTMSREDAKEKLEAAGAKVAGSVSKKTDYVVAGAEAGSKLEKAQTLGVAVLDEEGMLKLLAEVGA
A
>Mature_720_residues
TAKKQGAQASASAPSGDSPAERAQWLREELERYSYQYYVLDAPTIPDADYDALFIELQALEAEHPELLTPDSPTQRVGGA
PLSAFDSVRHRVPMLSLNNGFEDEDVINFDRRCAQGLGRATAAAPAAFATPAAPADDLFSAADAAEAAANTAGNAVEYAC
ELKFDGLAMSLRYENGTLVQAATRGDGETGEDVTANVRTIKAIPLKLRGTAPSVLEVRGEVFMYRADFDKLNARQAESGE
KTFVNPRNAAAGSLRQLDPRITAKRPLSFFAYGVGEMEGFDRPGTHSAMLDGFAKLGLPVCDERRVVRGAQGLLSFHREV
GERRDQLPYDIDGVVYKVNAIAEQEQLGFVSRAPRFALAHKFPAQEMTTTVEDIEVQVGRTGAITPVARLAPVFVGGVTV
TNATLHNEDEIRRKDVHIGDTVIVRRAGDVIPEVVAVVLERRPADARAFVMPTECPVCGSHVERLEGEAIARCTGGLICA
AQRKQALLHFAQRRAMDIEGLGDKVVEQLVDLGIVRTPADLYKLGVAKLAALDRMADKSASNLVAAIEQSRETTMNRFIF
ALGIRHVGEATAKDLAKHFGKLDNLLAADEAALLEVNDVGPVVAQSIANFIAEPHNVEVIEQLRAAGVHWAETEPTARAP
LPLAGKTFVLTGTLPTMSREDAKEKLEAAGAKVAGSVSKKTDYVVAGAEAGSKLEKAQTLGVAVLDEEGMLKLLAEVGAA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=73, Percent_Identity=47.945205479452, Blast_Score=70, Evalue=8e-12,
Organism=Escherichia coli, GI1788750, Length=699, Percent_Identity=57.653791130186, Blast_Score=775, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=404, Percent_Identity=25.7425742574257, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_RALME (Q1LNG5)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_583580.1
- ProteinModelPortal:   Q1LNG5
- STRING:   Q1LNG5
- GeneID:   4038231
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1428
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q1LNG5
- ProtClustDB:   CLSK2503705
- BioCyc:   RMET266264:RMET_1428-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 77306; Mature: 77175

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 164-164 BINDING 162-162 BINDING 185-185 BINDING 221-221 BINDING 338-338 BINDING 362-362

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKKQGAQASASAPSGDSPAERAQWLREELERYSYQYYVLDAPTIPDADYDALFIELQA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEEEE
LEAEHPELLTPDSPTQRVGGAPLSAFDSVRHRVPMLSLNNGFEDEDVINFDRRCAQGLGR
CCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHCCC
ATAAAPAAFATPAAPADDLFSAADAAEAAANTAGNAVEYACELKFDGLAMSLRYENGTLV
HHHCCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEEEECCCCEE
QAATRGDGETGEDVTANVRTIKAIPLKLRGTAPSVLEVRGEVFMYRADFDKLNARQAESG
EEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHCCCCEEEEECCHHHHCCCCCCCC
EKTFVNPRNAAAGSLRQLDPRITAKRPLSFFAYGVGEMEGFDRPGTHSAMLDGFAKLGLP
CCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCC
VCDERRVVRGAQGLLSFHREVGERRDQLPYDIDGVVYKVNAIAEQEQLGFVSRAPRFALA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCHHHCCCCHHHH
HKFPAQEMTTTVEDIEVQVGRTGAITPVARLAPVFVGGVTVTNATLHNEDEIRRKDVHIG
HCCCHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHCCCEEEEECEECCHHHHHHHCCCCC
DTVIVRRAGDVIPEVVAVVLERRPADARAFVMPTECPVCGSHVERLEGEAIARCTGGLIC
CEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHCCCEEE
AAQRKQALLHFAQRRAMDIEGLGDKVVEQLVDLGIVRTPADLYKLGVAKLAALDRMADKS
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ASNLVAAIEQSRETTMNRFIFALGIRHVGEATAKDLAKHFGKLDNLLAADEAALLEVNDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
GPVVAQSIANFIAEPHNVEVIEQLRAAGVHWAETEPTARAPLPLAGKTFVLTGTLPTMSR
CHHHHHHHHHHHCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCH
EDAKEKLEAAGAKVAGSVSKKTDYVVAGAEAGSKLEKAQTLGVAVLDEEGMLKLLAEVGA
HHHHHHHHHCCCHHHCCCCCCCCEEEECCCCCHHHHHHHHCCEEEECCCHHHHHHHHHCC
A
C
>Mature Secondary Structure 
TAKKQGAQASASAPSGDSPAERAQWLREELERYSYQYYVLDAPTIPDADYDALFIELQA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEEEE
LEAEHPELLTPDSPTQRVGGAPLSAFDSVRHRVPMLSLNNGFEDEDVINFDRRCAQGLGR
CCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHCCC
ATAAAPAAFATPAAPADDLFSAADAAEAAANTAGNAVEYACELKFDGLAMSLRYENGTLV
HHHCCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEEEECCCCEE
QAATRGDGETGEDVTANVRTIKAIPLKLRGTAPSVLEVRGEVFMYRADFDKLNARQAESG
EEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHCCCCEEEEECCHHHHCCCCCCCC
EKTFVNPRNAAAGSLRQLDPRITAKRPLSFFAYGVGEMEGFDRPGTHSAMLDGFAKLGLP
CCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCC
VCDERRVVRGAQGLLSFHREVGERRDQLPYDIDGVVYKVNAIAEQEQLGFVSRAPRFALA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCHHHCCCCHHHH
HKFPAQEMTTTVEDIEVQVGRTGAITPVARLAPVFVGGVTVTNATLHNEDEIRRKDVHIG
HCCCHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHCCCEEEEECEECCHHHHHHHCCCCC
DTVIVRRAGDVIPEVVAVVLERRPADARAFVMPTECPVCGSHVERLEGEAIARCTGGLIC
CEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHCCCEEE
AAQRKQALLHFAQRRAMDIEGLGDKVVEQLVDLGIVRTPADLYKLGVAKLAALDRMADKS
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ASNLVAAIEQSRETTMNRFIFALGIRHVGEATAKDLAKHFGKLDNLLAADEAALLEVNDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
GPVVAQSIANFIAEPHNVEVIEQLRAAGVHWAETEPTARAPLPLAGKTFVLTGTLPTMSR
CHHHHHHHHHHHCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCH
EDAKEKLEAAGAKVAGSVSKKTDYVVAGAEAGSKLEKAQTLGVAVLDEEGMLKLLAEVGA
HHHHHHHHHCCCHHHCCCCCCCCEEEECCCCCHHHHHHHHCCEEEECCCHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA