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The map label for this gene is dapE

Identifier: 94310362

GI number: 94310362

Start: 1534987

End: 1536138

Strand: Reverse

Name: dapE

Synonym: Rmet_1420

Alternate gene names: 94310362

Gene position: 1536138-1534987 (Counterclockwise)

Preceding gene: 94310363

Following gene: 94310361

Centisome position: 39.11

GC content: 65.71

Gene sequence:

>1152_bases
ATGAACCCGACCCTTGCCCTGACCGAAGACCTGATCCGCCGCCGCTCGGTCACCCCCGCCGACGAAGGTTGCCAGGCCGT
GCTCGAGACCCGACTCAAGGCCCTTGGCTTTACCTGCGAGAACATCGTCAGCGGCCCTGACGATTTCCGCGTCACCAACC
TGTGGGCGGTCAAGCGCGGCACCCAGGGCACGGCAGGCAAGCTGCTGGCCTTTGCCGGCCATACCGACGTAGTGCCGACC
GGCCCGCTCGACCAATGGTCATCGGACCCGTTCGAGCCCTCCCATCGCGATGGCAAGCTCTACGGTCGGGGCGCGGCCGA
CATGAAGACGTCGATTGCCGGCTTCGTGGTGGCGGTCGAGGAGTTCGTGGCGAAACACCCCGAGCACAAGGGATCGATCG
CCTTCCTGATCACGAGCGACGAGGAAGGCCCGGCGCATGACGGCACCGTGAAGGTGGTGGAAGCGCTGAAGGCACGCGGC
GAACGTCTCGACTATTGCGTGGTGGGCGAGCCGACCTCGGTCGACACGCTCGGCGACATGGTCAAGAACGGCCGTCGTGG
CTCGCTGTCCGGAAAGCTCACGGTCAAAGGTGTGCAAGGCCACATCGCCTACCCGCACCTGGCGCGCAACCCGATCCACC
TTGCCGCGCCGGCACTGACCACGCTGGTGAACGAGGTATGGGACGCGGGCAACGAGTATTTCCCGCCCACCACGTGGCAG
ATGTCGAATATCCATGGCGGCACCGGCGCGACCAACGTGATCCCGGGCCACGTGACGATCGATTTCAACTTCCGCTTCTC
GACGGCCAGCACGCCTGACGGCCTGAAGTCGCGCGTGCACGCAATCCTCGACCAGCACGGGCTCGACTACACGCTCGACT
GGACGCTGGGCGGCGAGCCGTTCCTGACGCCGCGCGGCGACCTGTCCGAAGCACTGGGCGCGGCCATCGCGGCCGAGACC
GGCGTGAAGACGGAACTCTCGACCACCGGCGGCACCTCCGACGGCCGTTTCATCGCGAAGATCTGCCCGCAGGTCATCGA
ATTCGGCCCGCCGAACGCCAGCATCCACAAGATCGACGAACACGTGGAAGTCCGCTTCGTCGAACCGCTCAAGAACGTTT
ACCGTGGCGTGCTGGAACGCCTGATTGCCTGA

Upstream 100 bases:

>100_bases
GCTGATCAAGCGTCCCGTGCTGGACCGCGACGGCAAGGTCAGCGTCGGTTTTTCCGCTGACGGCTACGCCGCCCTGTTCT
AACGATTCATCCCCCGCCCC

Downstream 100 bases:

>100_bases
TCCCCATGGCCACCACCTCTTCCCTGAAAACCGTCCGCGACATGCTGCGCCTGGCGGTTTCGCGCTTTACCGCCGCGAAG
CTCTCTTTCGGTCACGGCAG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MNPTLALTEDLIRRRSVTPADEGCQAVLETRLKALGFTCENIVSGPDDFRVTNLWAVKRGTQGTAGKLLAFAGHTDVVPT
GPLDQWSSDPFEPSHRDGKLYGRGAADMKTSIAGFVVAVEEFVAKHPEHKGSIAFLITSDEEGPAHDGTVKVVEALKARG
ERLDYCVVGEPTSVDTLGDMVKNGRRGSLSGKLTVKGVQGHIAYPHLARNPIHLAAPALTTLVNEVWDAGNEYFPPTTWQ
MSNIHGGTGATNVIPGHVTIDFNFRFSTASTPDGLKSRVHAILDQHGLDYTLDWTLGGEPFLTPRGDLSEALGAAIAAET
GVKTELSTTGGTSDGRFIAKICPQVIEFGPPNASIHKIDEHVEVRFVEPLKNVYRGVLERLIA

Sequences:

>Translated_383_residues
MNPTLALTEDLIRRRSVTPADEGCQAVLETRLKALGFTCENIVSGPDDFRVTNLWAVKRGTQGTAGKLLAFAGHTDVVPT
GPLDQWSSDPFEPSHRDGKLYGRGAADMKTSIAGFVVAVEEFVAKHPEHKGSIAFLITSDEEGPAHDGTVKVVEALKARG
ERLDYCVVGEPTSVDTLGDMVKNGRRGSLSGKLTVKGVQGHIAYPHLARNPIHLAAPALTTLVNEVWDAGNEYFPPTTWQ
MSNIHGGTGATNVIPGHVTIDFNFRFSTASTPDGLKSRVHAILDQHGLDYTLDWTLGGEPFLTPRGDLSEALGAAIAAET
GVKTELSTTGGTSDGRFIAKICPQVIEFGPPNASIHKIDEHVEVRFVEPLKNVYRGVLERLIA
>Mature_383_residues
MNPTLALTEDLIRRRSVTPADEGCQAVLETRLKALGFTCENIVSGPDDFRVTNLWAVKRGTQGTAGKLLAFAGHTDVVPT
GPLDQWSSDPFEPSHRDGKLYGRGAADMKTSIAGFVVAVEEFVAKHPEHKGSIAFLITSDEEGPAHDGTVKVVEALKARG
ERLDYCVVGEPTSVDTLGDMVKNGRRGSLSGKLTVKGVQGHIAYPHLARNPIHLAAPALTTLVNEVWDAGNEYFPPTTWQ
MSNIHGGTGATNVIPGHVTIDFNFRFSTASTPDGLKSRVHAILDQHGLDYTLDWTLGGEPFLTPRGDLSEALGAAIAAET
GVKTELSTTGGTSDGRFIAKICPQVIEFGPPNASIHKIDEHVEVRFVEPLKNVYRGVLERLIA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Homo sapiens, GI4501901, Length=383, Percent_Identity=24.0208877284595, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1788816, Length=382, Percent_Identity=57.5916230366492, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1790395, Length=407, Percent_Identity=25.3071253071253, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_RALME (Q1LNH3)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_583572.1
- ProteinModelPortal:   Q1LNH3
- SMR:   Q1LNH3
- STRING:   Q1LNH3
- MEROPS:   M20.010
- GeneID:   4038223
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1420
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   ARNPVHQ
- PhylomeDB:   Q1LNH3
- ProtClustDB:   PRK13009
- BioCyc:   RMET266264:RMET_1420-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41175; Mature: 41175

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 76-76 ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTLALTEDLIRRRSVTPADEGCQAVLETRLKALGFTCENIVSGPDDFRVTNLWAVKRG
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEEECC
TQGTAGKLLAFAGHTDVVPTGPLDQWSSDPFEPSHRDGKLYGRGAADMKTSIAGFVVAVE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHH
EFVAKHPEHKGSIAFLITSDEEGPAHDGTVKVVEALKARGERLDYCVVGEPTSVDTLGDM
HHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
VKNGRRGSLSGKLTVKGVQGHIAYPHLARNPIHLAAPALTTLVNEVWDAGNEYFPPTTWQ
HHCCCCCCCCCEEEEEECCCEEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEE
MSNIHGGTGATNVIPGHVTIDFNFRFSTASTPDGLKSRVHAILDQHGLDYTLDWTLGGEP
ECCCCCCCCCCCEECCEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCC
FLTPRGDLSEALGAAIAAETGVKTELSTTGGTSDGRFIAKICPQVIEFGPPNASIHKIDE
CCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHCC
HVEVRFVEPLKNVYRGVLERLIA
HHEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPTLALTEDLIRRRSVTPADEGCQAVLETRLKALGFTCENIVSGPDDFRVTNLWAVKRG
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEEECC
TQGTAGKLLAFAGHTDVVPTGPLDQWSSDPFEPSHRDGKLYGRGAADMKTSIAGFVVAVE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHH
EFVAKHPEHKGSIAFLITSDEEGPAHDGTVKVVEALKARGERLDYCVVGEPTSVDTLGDM
HHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
VKNGRRGSLSGKLTVKGVQGHIAYPHLARNPIHLAAPALTTLVNEVWDAGNEYFPPTTWQ
HHCCCCCCCCCEEEEEECCCEEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEE
MSNIHGGTGATNVIPGHVTIDFNFRFSTASTPDGLKSRVHAILDQHGLDYTLDWTLGGEP
ECCCCCCCCCCCEECCEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCC
FLTPRGDLSEALGAAIAAETGVKTELSTTGGTSDGRFIAKICPQVIEFGPPNASIHKIDE
CCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHCC
HVEVRFVEPLKNVYRGVLERLIA
HHEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA