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The map label for this gene is dmpD [H]

Identifier: 94310250

GI number: 94310250

Start: 1421155

End: 1421985

Strand: Reverse

Name: dmpD [H]

Synonym: Rmet_1306

Alternate gene names: 94310250

Gene position: 1421985-1421155 (Counterclockwise)

Preceding gene: 335055523

Following gene: 94310249

Centisome position: 36.2

GC content: 56.92

Gene sequence:

>831_bases
ATGTCGGATTCTTTAGAAATTGCGAATACAGTCACCGCAAATGGTATAAAAACCAATTATCACGATCGTGGTCAGGGAGT
GCCTGTTCTTTTGATTCATGGATCGGGCCCTGGAGTAACCGCATGGGCGAACTGGCGCCTCACAATCCCTGAACTCGCGA
AGCAGTTTCGCGTCATCGCACCGGACATGGTGGGCTTTGGATATACCGAGCGGCCGGTGGGTATCCGGTATGACATGGAT
ACCTGGGTCGGGCATGCGCTCGGGTTGCTGGATGCGCTTGGCATTGACAAGGCTCACGTGGTGGGCAACTCTTTCGGAGG
GGCTCTGGCCTTGGCGCTGGCCATCCGGGCGCCCGAAAGAGTGCGCCGATTGGTCCTCATGGGCAGCGTGGGTATCTCCT
TCCCGATAACGGAGGGGTTGGACAAGGTTTGGGGCTACGTTCCGTCAATCGACAACATGCGAGAGTTGCTCGATGTTTTT
GCGTTCGACCGCAATCTCGTAAATGACGACCTCGCTCGCCTGCGTTATGAGGCAAGCATCCGGCCGAACTTTCAGGAAGC
GTTTTCAAGTATGTTTCCGGCACCGCGCCAGCGTTGGGTGGACGCTATGGCGAGCCGGGAGGAGGACGTCGGCTCGCTTC
CGCACGAGACACTCATCATCCATGGGCGCGAAGACCAGGTCATCCCGCTGTCCAATTCCTATACACTGCTCAGACTGATT
CCACGGGCGCAGCTGCACGTGTTCGGCCGCTGTGGCCATTGGACGCAGATTGAACATGGAGAGCGATTTAACCGGTTGGT
CGGTGACTTCTTCTCGGAAGAGGCGCTTTAA

Upstream 100 bases:

>100_bases
AGAAAGATCGATGATGATTCAACCAAGAAAAATCATACGAGGTTGTTAAGGGAAATCAAGTTATTGCTCGTTGCCGGATA
CTGGATATATGGAGTTGCAA

Downstream 100 bases:

>100_bases
GCCTCCGCGCGGAGGCCTTTGGGGGGCGTTCAAGTGACGCCAACGGCATGTGGCAACGAACGGTCCCCCAATTAGGTTTT
CGTTCCAGGCAGGTTTACGC

Product: 2-hydroxymuconic semialdehyde hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: HMSH [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSDSLEIANTVTANGIKTNYHDRGQGVPVLLIHGSGPGVTAWANWRLTIPELAKQFRVIAPDMVGFGYTERPVGIRYDMD
TWVGHALGLLDALGIDKAHVVGNSFGGALALALAIRAPERVRRLVLMGSVGISFPITEGLDKVWGYVPSIDNMRELLDVF
AFDRNLVNDDLARLRYEASIRPNFQEAFSSMFPAPRQRWVDAMASREEDVGSLPHETLIIHGREDQVIPLSNSYTLLRLI
PRAQLHVFGRCGHWTQIEHGERFNRLVGDFFSEEAL

Sequences:

>Translated_276_residues
MSDSLEIANTVTANGIKTNYHDRGQGVPVLLIHGSGPGVTAWANWRLTIPELAKQFRVIAPDMVGFGYTERPVGIRYDMD
TWVGHALGLLDALGIDKAHVVGNSFGGALALALAIRAPERVRRLVLMGSVGISFPITEGLDKVWGYVPSIDNMRELLDVF
AFDRNLVNDDLARLRYEASIRPNFQEAFSSMFPAPRQRWVDAMASREEDVGSLPHETLIIHGREDQVIPLSNSYTLLRLI
PRAQLHVFGRCGHWTQIEHGERFNRLVGDFFSEEAL
>Mature_275_residues
SDSLEIANTVTANGIKTNYHDRGQGVPVLLIHGSGPGVTAWANWRLTIPELAKQFRVIAPDMVGFGYTERPVGIRYDMDT
WVGHALGLLDALGIDKAHVVGNSFGGALALALAIRAPERVRRLVLMGSVGISFPITEGLDKVWGYVPSIDNMRELLDVFA
FDRNLVNDDLARLRYEASIRPNFQEAFSSMFPAPRQRWVDAMASREEDVGSLPHETLIIHGREDQVIPLSNSYTLLRLIP
RAQLHVFGRCGHWTQIEHGERFNRLVGDFFSEEAL

Specific function: Conversion of 2-hydroxymuconic acid semialdehyde to 2- hydroxypent-2,4-dienoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI27597073, Length=184, Percent_Identity=29.3478260869565, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI218777837, Length=292, Percent_Identity=23.2876712328767, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI87081721, Length=270, Percent_Identity=35.5555555555556, Blast_Score=145, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324392, Length=135, Percent_Identity=34.8148148148148, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 30789; Mature: 30657

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSLEIANTVTANGIKTNYHDRGQGVPVLLIHGSGPGVTAWANWRLTIPELAKQFRVIA
CCCCCCHHHEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEEHHHHHHHHHHHC
PDMVGFGYTERPVGIRYDMDTWVGHALGLLDALGIDKAHVVGNSFGGALALALAIRAPER
CHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHCCHHH
VRRLVLMGSVGISFPITEGLDKVWGYVPSIDNMRELLDVFAFDRNLVNDDLARLRYEASI
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
RPNFQEAFSSMFPAPRQRWVDAMASREEDVGSLPHETLIIHGREDQVIPLSNSYTLLRLI
CCCHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCEEECCCCCEEEEEC
PRAQLHVFGRCGHWTQIEHGERFNRLVGDFFSEEAL
CHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDSLEIANTVTANGIKTNYHDRGQGVPVLLIHGSGPGVTAWANWRLTIPELAKQFRVIA
CCCCCHHHEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEEHHHHHHHHHHHC
PDMVGFGYTERPVGIRYDMDTWVGHALGLLDALGIDKAHVVGNSFGGALALALAIRAPER
CHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHCCHHH
VRRLVLMGSVGISFPITEGLDKVWGYVPSIDNMRELLDVFAFDRNLVNDDLARLRYEASI
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
RPNFQEAFSSMFPAPRQRWVDAMASREEDVGSLPHETLIIHGREDQVIPLSNSYTLLRLI
CCCHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCEEECCCCCEEEEEC
PRAQLHVFGRCGHWTQIEHGERFNRLVGDFFSEEAL
CHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2194577 [H]