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The map label for this gene is hupE [H]

Identifier: 94310240

GI number: 94310240

Start: 1411714

End: 1412451

Strand: Reverse

Name: hupE [H]

Synonym: Rmet_1296

Alternate gene names: 94310240

Gene position: 1412451-1411714 (Counterclockwise)

Preceding gene: 94310241

Following gene: 94310239

Centisome position: 35.96

GC content: 70.05

Gene sequence:

>738_bases
ATGAGCCCCCGCCTGCGGCGCTTCGCGTCTTCCCCCCGAAGGGGGGACGCACCTGGTGGTCCGGCGGAGCCGGTTCCAAG
GGTGCCCTGGACAAGACCGCCGCACCGCCATGAACAGACAACGAGGAGAACAACGATGAAGAAACAGACAGCGCGCGGCC
TGATGGCCGCCACCTTGCTCGCCGGCGTGGCCGGCGCCGCACAGGCCCACACCGGCCACGGCACCAGCAGCCTGTTCGAA
GGCCTGGTGCACCCCTTCGGTCCTGACCACCTGCTGGCCATGGTCGCCGTGGGTGTGTGGTCGGTCTCGGCGCTGCCCGC
GAACAAGGCCTGGCAAGGTCCGGCCGTGTTCCTGCTGGCGCTCATTGCGAGCGCAACGCTGGGCATGCTCGGCGTGACGG
TGCCGTACCTGGAGCACGGGGTCTCGCTCTCCGTGGTGATGTTCGGTGCCATGCTGTTGCTCGCAGGGCGTGCCAGCGTG
CCACCCGCGGTGGGCCTGGGGCTGATCGCCGCAGCCGCTTCGCTGCACGGCCTGGCACACGGCTCCGAAACGCCGGAGAG
CGGTTTCGCCGGCTATGCGATCGGCTTCCTGCTCACCACAGCGGTGTTGCACCTGGGCGGTGTCGGCATCGGCCTGGGCA
TTCGGCGCTGGCTGGCCGAGCGCAGCGGTTGGGTGCTCAGCGGCCTGGGCGCCATGCTGAGCGCGGCGGGCCTTTACCTG
TTCGGCCAGCTCGCCTGA

Upstream 100 bases:

>100_bases
TGGAGATCCTGCGCACGCTGCACAGCTTCGACCCTTGCCTGGCTTGCTCCACCCACGTGATGAGCGAAGACGGCCAGGAG
ATGAGCCGGGTGAAGGTGAG

Downstream 100 bases:

>100_bases
ACGCCCTGCACAACCGCCATCGAAGGAGGTCATCGCCATGTCCGCCACATCCCCTTCCGCACGCACCAGCGCCGCCGATC
ACGACGCGCTGGAATTGCAG

Product: putative membrane-bound hydrogenase component HupE/UreJ protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSPRLRRFASSPRRGDAPGGPAEPVPRVPWTRPPHRHEQTTRRTTMKKQTARGLMAATLLAGVAGAAQAHTGHGTSSLFE
GLVHPFGPDHLLAMVAVGVWSVSALPANKAWQGPAVFLLALIASATLGMLGVTVPYLEHGVSLSVVMFGAMLLLAGRASV
PPAVGLGLIAAAASLHGLAHGSETPESGFAGYAIGFLLTTAVLHLGGVGIGLGIRRWLAERSGWVLSGLGAMLSAAGLYL
FGQLA

Sequences:

>Translated_245_residues
MSPRLRRFASSPRRGDAPGGPAEPVPRVPWTRPPHRHEQTTRRTTMKKQTARGLMAATLLAGVAGAAQAHTGHGTSSLFE
GLVHPFGPDHLLAMVAVGVWSVSALPANKAWQGPAVFLLALIASATLGMLGVTVPYLEHGVSLSVVMFGAMLLLAGRASV
PPAVGLGLIAAAASLHGLAHGSETPESGFAGYAIGFLLTTAVLHLGGVGIGLGIRRWLAERSGWVLSGLGAMLSAAGLYL
FGQLA
>Mature_244_residues
SPRLRRFASSPRRGDAPGGPAEPVPRVPWTRPPHRHEQTTRRTTMKKQTARGLMAATLLAGVAGAAQAHTGHGTSSLFEG
LVHPFGPDHLLAMVAVGVWSVSALPANKAWQGPAVFLLALIASATLGMLGVTVPYLEHGVSLSVVMFGAMLLLAGRASVP
PAVGLGLIAAAASLHGLAHGSETPESGFAGYAIGFLLTTAVLHLGGVGIGLGIRRWLAERSGWVLSGLGAMLSAAGLYLF
GQLA

Specific function: Needed for full hydrogenase activity [H]

COG id: COG2370

COG function: function code O; Hydrogenase/urease accessory protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007038 [H]

Pfam domain/function: PF04955 HupE_UreJ [H]

EC number: NA

Molecular weight: Translated: 25180; Mature: 25049

Theoretical pI: Translated: 11.77; Mature: 11.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCC
>Mature Secondary Structure 
SPRLRRFASSPRRGDAPGGPAEPVPRVPWTRPPHRHEQTTRRTTMKKQTARGLMAATLL
CHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGVAGAAQAHTGHGTSSLFEGLVHPFGPDHLLAMVAVGVWSVSALPANKAWQGPAVFLLA
HHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LIASATLGMLGVTVPYLEHGVSLSVVMFGAMLLLAGRASVPPAVGLGLIAAAASLHGLAH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
GSETPESGFAGYAIGFLLTTAVLHLGGVGIGLGIRRWLAERSGWVLSGLGAMLSAAGLYL
CCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
FGQLA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1597428 [H]