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The map label for this gene is yagI [H]

Identifier: 94310150

GI number: 94310150

Start: 1324582

End: 1325367

Strand: Reverse

Name: yagI [H]

Synonym: Rmet_1205

Alternate gene names: 94310150

Gene position: 1325367-1324582 (Counterclockwise)

Preceding gene: 94310154

Following gene: 94310145

Centisome position: 33.74

GC content: 65.01

Gene sequence:

>786_bases
ATGGCAGAAGCAGACAAGTCACCCGGCAAGACCTCGATCCAGGTGATCGAGCGCATGATGACGCTGCTGGACGCGCTGGC
CCAGCACGCCGACCCCGTCAGTCTGAAAGAGCTTTCGCTGGCCACCGGCCTGCACCCGTCCACGGCCCACCGGATTCTGA
ACGACATGGTGGCGTGCCGCTTTGTCGACCGCTCCGATCCGGGCAGCTATCGACTGGGTATGCGGCTGCTCGAGCTGGGC
AATCTGGTCAAGGCACGCCTTTCGGTGCGTGACGCTGCACTGGCACCAATGCGTGCCCTGCACCGCGTCACCGGCCAGAC
CGTCAACCTGTCGGTACGTCAGGGCGACGAAATCGTCTACATCGAGCGCGCCTACAGCGAGCGCTCGGGGATGCAGGTGG
TGCGTGCCATCGGCGGCCGCGCCCCGCTGCACCTGACCTCGGTCGGCAAACTGTTCCTGGCCGCGGACGAAGGCGCGCGT
GTACGCAACTACGCCACCCGTACCGGACTGGCCGGCCATACGCGCACGTCGATCACGGACATCTCCAAGCTCGAACGCGA
ACTGACCTGGGTGCGCTCGAATGGCTACGCACGCGATAACGAGGAACTGGAACTCGGCGTCCGCTGTATCGCCGCAGGCA
TCTATGACGACTCGCGGCGCCTTGTCGCCGGCCTGTCACTCTCTGCCCCGGCCGATCGGCTTCAGGATAGCTGGCTGCAG
AACCTCAAGGACACCGCGCTGCAGATCTCGCGTGGCATGGGATATGTCCCCGAGCCTACAGCTTGA

Upstream 100 bases:

>100_bases
GAGGTTGATTTCAGTAATCGCGTTTCATCAGTTGAAATTTTCACGATGTGAGATAGAATCAGGCAAACGCTTAATTTGCC
GCACGGCACAAATTTTCTCC

Downstream 100 bases:

>100_bases
TCGCCTGATCGCAGCCGAGGCAAACAAAAAACGCGCCGCCGGGTTTTCGCCCGGCGGCGCGTTTTTCTTGTACGGCCCAA
ACGTTCGGATGTCGTCGCTC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MAEADKSPGKTSIQVIERMMTLLDALAQHADPVSLKELSLATGLHPSTAHRILNDMVACRFVDRSDPGSYRLGMRLLELG
NLVKARLSVRDAALAPMRALHRVTGQTVNLSVRQGDEIVYIERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAADEGAR
VRNYATRTGLAGHTRTSITDISKLERELTWVRSNGYARDNEELELGVRCIAAGIYDDSRRLVAGLSLSAPADRLQDSWLQ
NLKDTALQISRGMGYVPEPTA

Sequences:

>Translated_261_residues
MAEADKSPGKTSIQVIERMMTLLDALAQHADPVSLKELSLATGLHPSTAHRILNDMVACRFVDRSDPGSYRLGMRLLELG
NLVKARLSVRDAALAPMRALHRVTGQTVNLSVRQGDEIVYIERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAADEGAR
VRNYATRTGLAGHTRTSITDISKLERELTWVRSNGYARDNEELELGVRCIAAGIYDDSRRLVAGLSLSAPADRLQDSWLQ
NLKDTALQISRGMGYVPEPTA
>Mature_260_residues
AEADKSPGKTSIQVIERMMTLLDALAQHADPVSLKELSLATGLHPSTAHRILNDMVACRFVDRSDPGSYRLGMRLLELGN
LVKARLSVRDAALAPMRALHRVTGQTVNLSVRQGDEIVYIERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAADEGARV
RNYATRTGLAGHTRTSITDISKLERELTWVRSNGYARDNEELELGVRCIAAGIYDDSRRLVAGLSLSAPADRLQDSWLQN
LKDTALQISRGMGYVPEPTA

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=253, Percent_Identity=34.3873517786561, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1789999, Length=263, Percent_Identity=31.1787072243346, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788131, Length=264, Percent_Identity=32.1969696969697, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1786715, Length=251, Percent_Identity=31.8725099601594, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI87082359, Length=236, Percent_Identity=32.6271186440678, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1790752, Length=217, Percent_Identity=29.9539170506912, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1788581, Length=260, Percent_Identity=26.1538461538462, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28591; Mature: 28460

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEADKSPGKTSIQVIERMMTLLDALAQHADPVSLKELSLATGLHPSTAHRILNDMVACR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
FVDRSDPGSYRLGMRLLELGNLVKARLSVRDAALAPMRALHRVTGQTVNLSVRQGDEIVY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEE
IERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAADEGARVRNYATRTGLAGHTRTSITD
EEECCCCHHHHHHHHHHCCCCCEEEHHHCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHH
ISKLERELTWVRSNGYARDNEELELGVRCIAAGIYDDSRRLVAGLSLSAPADRLQDSWLQ
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHEECCCCCCCHHHHHHHHHH
NLKDTALQISRGMGYVPEPTA
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AEADKSPGKTSIQVIERMMTLLDALAQHADPVSLKELSLATGLHPSTAHRILNDMVACR
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
FVDRSDPGSYRLGMRLLELGNLVKARLSVRDAALAPMRALHRVTGQTVNLSVRQGDEIVY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEE
IERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAADEGARVRNYATRTGLAGHTRTSITD
EEECCCCHHHHHHHHHHCCCCCEEEHHHCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHH
ISKLERELTWVRSNGYARDNEELELGVRCIAAGIYDDSRRLVAGLSLSAPADRLQDSWLQ
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHEECCCCCCCHHHHHHHHHH
NLKDTALQISRGMGYVPEPTA
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]