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The map label for this gene is tetA [H]

Identifier: 94310059

GI number: 94310059

Start: 1214621

End: 1215808

Strand: Reverse

Name: tetA [H]

Synonym: Rmet_1114

Alternate gene names: 94310059

Gene position: 1215808-1214621 (Counterclockwise)

Preceding gene: 94310060

Following gene: 94310058

Centisome position: 30.95

GC content: 68.52

Gene sequence:

>1188_bases
ATGCGTCCCAATCGCCCCCAAACGCTGACAGTGCTGCTGACCACACAGGCGGTGGTCACCACGGGTCTGATGACGCTCGT
GCCGATTCTGCCGCTCTACCTGCAAGCCATGCCGGGCACGCATGGCACCGACTGGAGCAGTGTCGCGCTGGCCGCGCCCG
CGGTCGGGGCGCTCGCGGTGGCGCCGTTCACCGGCATCTGGTGCGATCGCGTCGGTCATCGCCCGGTCTTGCTCGTGTCA
CTGGTCGTCTTCACGGTCAGCGTGCTGATCCTGGCCACTTCGCAGCATGTCGCCGGATTCATCGTCGGTCGGGTATTGCA
AGGCGCCAGCGCCATCGGCGTGATGCTGACCGCATGCATCGGACACGTTAGCGCGCCCGCCTCGCGCAACTGGGCGTTTG
GCCTTCAGGAGTCGGCGGTGGCAGCCGGCGCACTGCTGGGCCCGGTGCTCGGCGGCGTGTTGCAGGATGTCTGGTCACCA
CGTCCGATGCTGTTTGCGGTGGCGGCGCTCAATGCCTTGCTGACGTTAGCGCTGTCGACCCTGTTGCGCGACATGCCTGC
GCACACCGCGCCGGTGCCTTCCACTGGCAAGCGTCGGCCCATCCGCGATGTGCTGATGCTGCGCCGCTGGCTGGCCGCCG
CCTGTCTGTCGCAGGCGGGCGCGTTCGCGCTCGTCAACGTGTTCGTGCTCTACCTGCAGCAGCGCGCGCCGCACGATGCG
CTGCTAGCCAGCAAAGCCGGCACGCTCCACGCGCTCGGCTGGCTCGCGGCGATGTTCGCCAGCCCCTGGTGGGGCGCGCG
CAATGATCGCGTGGCGTCCGGGCGGCATTTTCTCCTTGCCGCATCCGGATGCGCGGCGGCCATTGCACTGCTGCCGCTGG
CCACCGCGCTCTGGCAGATCGCGCTGCTCCGCGTGATTCACGGCGCGCTGTTTGCCGCACTGGCGCTGTCCGTGCTCGCC
CATTGCCATCGGGTGCTTCCCGCAGCGCTGCACGGCACCGCCACGGGCGTGGCGCGAAGCGCGATGACGGCCGGCCAACT
CGTCGGACCGCTGGCCGTGATGGTCAGTCTGCCGCTCGGTGGCCCGAACGCCGCGCTCTGGGTAACGGCCGCCATGTTTG
CCGTATCTGCCCTGCTGATCCTGCCAGACCGTTCTCCTTCATTCGCCCCTGTCCATGATGCGAGGTAA

Upstream 100 bases:

>100_bases
AAGTGCTCGCGCCATTGCTCGCCCGCGAAGCGATCGGCACGGGAATGCCCAGTGCGATGAGCAGCCTGCCGAATCCGCTA
CGCCAGCCGGAGCCATAGTC

Downstream 100 bases:

>100_bases
CACCGTGTCAATACTCTCAGTCGAACCTGCAGTCTCCGTGCTGCACGCCAAACGTGCTTTCTCCGCGACAACCCCATCTG
TCGATCCGGTGGAGCGGCAC

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: TetA(A) [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRPNRPQTLTVLLTTQAVVTTGLMTLVPILPLYLQAMPGTHGTDWSSVALAAPAVGALAVAPFTGIWCDRVGHRPVLLVS
LVVFTVSVLILATSQHVAGFIVGRVLQGASAIGVMLTACIGHVSAPASRNWAFGLQESAVAAGALLGPVLGGVLQDVWSP
RPMLFAVAALNALLTLALSTLLRDMPAHTAPVPSTGKRRPIRDVLMLRRWLAAACLSQAGAFALVNVFVLYLQQRAPHDA
LLASKAGTLHALGWLAAMFASPWWGARNDRVASGRHFLLAASGCAAAIALLPLATALWQIALLRVIHGALFAALALSVLA
HCHRVLPAALHGTATGVARSAMTAGQLVGPLAVMVSLPLGGPNAALWVTAAMFAVSALLILPDRSPSFAPVHDAR

Sequences:

>Translated_395_residues
MRPNRPQTLTVLLTTQAVVTTGLMTLVPILPLYLQAMPGTHGTDWSSVALAAPAVGALAVAPFTGIWCDRVGHRPVLLVS
LVVFTVSVLILATSQHVAGFIVGRVLQGASAIGVMLTACIGHVSAPASRNWAFGLQESAVAAGALLGPVLGGVLQDVWSP
RPMLFAVAALNALLTLALSTLLRDMPAHTAPVPSTGKRRPIRDVLMLRRWLAAACLSQAGAFALVNVFVLYLQQRAPHDA
LLASKAGTLHALGWLAAMFASPWWGARNDRVASGRHFLLAASGCAAAIALLPLATALWQIALLRVIHGALFAALALSVLA
HCHRVLPAALHGTATGVARSAMTAGQLVGPLAVMVSLPLGGPNAALWVTAAMFAVSALLILPDRSPSFAPVHDAR
>Mature_395_residues
MRPNRPQTLTVLLTTQAVVTTGLMTLVPILPLYLQAMPGTHGTDWSSVALAAPAVGALAVAPFTGIWCDRVGHRPVLLVS
LVVFTVSVLILATSQHVAGFIVGRVLQGASAIGVMLTACIGHVSAPASRNWAFGLQESAVAAGALLGPVLGGVLQDVWSP
RPMLFAVAALNALLTLALSTLLRDMPAHTAPVPSTGKRRPIRDVLMLRRWLAAACLSQAGAFALVNVFVLYLQQRAPHDA
LLASKAGTLHALGWLAAMFASPWWGARNDRVASGRHFLLAASGCAAAIALLPLATALWQIALLRVIHGALFAALALSVLA
HCHRVLPAALHGTATGVARSAMTAGQLVGPLAVMVSLPLGGPNAALWVTAAMFAVSALLILPDRSPSFAPVHDAR

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=340, Percent_Identity=23.5294117647059, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41000; Mature: 41000

Theoretical pI: Translated: 11.87; Mature: 11.87

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC
>Mature Secondary Structure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CCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312499; 6310527 [H]