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The map label for this gene is uvrB [H]

Identifier: 94309964

GI number: 94309964

Start: 1109264

End: 1111357

Strand: Direct

Name: uvrB [H]

Synonym: Rmet_1019

Alternate gene names: 94309964

Gene position: 1109264-1111357 (Clockwise)

Preceding gene: 94309961

Following gene: 94309965

Centisome position: 28.24

GC content: 63.47

Gene sequence:

>2094_bases
ATGACGAACCTTGCTGAAGCCGCCCCCGCCCTGGATGAAGACAAATTCGTCACGTTTCCGGGGTCTCCATTCCAGCTTTA
TCAGCCGTATTCGCCGGCGGGCGACCAGCCGGAGGCGATCCGCCAACTGGTGGAGGGCGTGGAGGACGGCCTGTCGTACC
AGACGCTGCTGGGCGTGACCGGCTCCGGCAAGACCTTCACGATGGCCAACGTGATCGCCCGGATGGGCCGGCCGGCCATT
GTCTTCGCGCCAAACAAGACGCTGGCGGCACAGCTGTATTCCGAGTTCCGGGAGTTCTTCCCGCGCAATGCGGTCGAGTA
TTTCGTCAGCTACTACGACTACTACCAGCCCGAAGCCTACGTGCCGCAGCGCGACCTGTTCATCGAAAAGGACTCGTCGA
TCAACGAGCATATCGAGCAGATGCGGCTGTCGGCGACCAAGAGCCTGCTGGAGCGGCGCGACACGATCATCGTCGCCACG
GTGTCGGCGATCTACGGTATCGGCAATCCGAGCGAATACCACCAGATGATCCTGACGCTGCGTACTGGCGACAAGATCAG
CCAGCGCGATGTCATCGCCCGCCTGATCGCGATGCAGTACACGCGCAACGAGACCGACTTCCAGCGCGGCACGTTCCGCG
TGCGCGGCGACACGCTCGACATCTTTCCGGCCGAGCACGCCGAGATGGCGGTGCGCGTCGAACTGTTCGACGACGAGGTG
GATTCGCTGCAGTTCTTCGATCCGCTGACCGGCCGCGTGCGCCAGAAGATTCCGCGTTTCACGGTCTATCCCTCGAGCCA
CTACGTGACGCCGCGCGAAACGGTGCTGCGCGCGATCGAGGACATCAAGGTGGAGCTGCGCGAGCGGCTGGATTTCTTCT
ACAAGGAGAACCGCCTCGTAGAGGCGCAGCGGCTCGAGCAGCGTACGCGGTTCGATCTGGAGATGCTCACCGAACTGGGC
TTCTGCAAGGGCATCGAGAACTATTCGCGCCATCTGTCGGGCGCGAAGCCGGGCGAGCCGCCGCCGACGCTGGTTGACTA
TCTGCCGCCAGACGCGCTGATGTTCCTGGACGAGTCCCACGTGCTGATCGGCCAGTTAAACGGCATGTACAACGGCGACC
GCGCGCGCAAGACCACGCTGGTCGAGTACGGCTTCCGCTTGCCCTCGGCGCTGGACAACCGACCGCTCAAGTTCGACGAG
TACGAGCGCAAGATGCGCCAGGCGGTGTTCGTGACAGCCACTCCGGCGAACTACGAGAAGGAGCACGCGGGGCAGGTGGT
CGAGCAGGTGGTGCGGCCGACGGGCCTGGTCGATCCGGTGATCGAGGTGCGGCCCGCCACGACGCAGGTGGACGACCTGC
TTGGGGAGATTCATCAGCGGGTCAAGGTCAATGAGCGTGTACTGGTAACGACGCTGACCAAGCGCATGGCGGAGCAACTG
ACCGAATTCCTTTCGGAAAACGGCGTGAAGGTCCGCTACCTGCATTCGGACATCGATACCGTCGAGCGCGTGGAAATCAT
TCGCGACCTGCGGCTTGGCACGTTCGACGTGCTCGTGGGTATCAACCTGCTGCGCGAGGGGCTCGACATCCCCGAGGTGT
CGCTGGTGGCGATCCTCGATGCGGACAAGGAGGGCTTCCTGCGCGCCGAGCGTTCTCTGATCCAGACCATCGGGCGCGCC
GCGCGTAACGTCAATGGCACGGCCATCCTGTACGGCGATCGTATGACCGACTCGATGAAGGTGGCGATCCGGGAAACCGA
GCGTCGCCGCGAGAAGCAGATGGCCTTCAACGAAGCCAACGGCATCACGCCGCGAGGCGTGGTCAAGCGCATCAAGGACA
TCATCGACGGGGTCTACAACGCCGACGACGTCAAGGCCGAACTGACGGCCGCCGCCGAGCGGGCCAGGTACGAGGACATG
AGCGAAAAGCAGGTGGCCAAGGAGATCAAGCGCCTGGAGAAGCAGATGCTCGACCACGCCAAGAACCTGGAGTTCGAAAA
GGCGGCCCAGGTGCGCGATCAACTCGCGAAGCTGCGGTCGCAGGTGTTCGGGGCTGGGGCCGAAGGCGATCATCTCCTGC
CGCCGTCCGCGTAA

Upstream 100 bases:

>100_bases
AACCTGACAGCAGCGTCGGGCAGACTGTGCTTGAACCAAGCCGCGCAACCCCAAGATTCTAGCGTATCCGCCAGTTTTCC
TCAGGTATTTCCCTACGTTT

Downstream 100 bases:

>100_bases
GAGGTCGATCGGGGATCACCCCGAGCCACGGCGTATCGCCGGCATCCTATAGTGGTTGGGGCGGAGACGCCGGATCACGG
ACAAGGAGCACACCATGGTG

Product: excinuclease ABC subunit B

Products: NA

Alternate protein names: Protein uvrB; Excinuclease ABC subunit B [H]

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MTNLAEAAPALDEDKFVTFPGSPFQLYQPYSPAGDQPEAIRQLVEGVEDGLSYQTLLGVTGSGKTFTMANVIARMGRPAI
VFAPNKTLAAQLYSEFREFFPRNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQMRLSATKSLLERRDTIIVAT
VSAIYGIGNPSEYHQMILTLRTGDKISQRDVIARLIAMQYTRNETDFQRGTFRVRGDTLDIFPAEHAEMAVRVELFDDEV
DSLQFFDPLTGRVRQKIPRFTVYPSSHYVTPRETVLRAIEDIKVELRERLDFFYKENRLVEAQRLEQRTRFDLEMLTELG
FCKGIENYSRHLSGAKPGEPPPTLVDYLPPDALMFLDESHVLIGQLNGMYNGDRARKTTLVEYGFRLPSALDNRPLKFDE
YERKMRQAVFVTATPANYEKEHAGQVVEQVVRPTGLVDPVIEVRPATTQVDDLLGEIHQRVKVNERVLVTTLTKRMAEQL
TEFLSENGVKVRYLHSDIDTVERVEIIRDLRLGTFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRAERSLIQTIGRA
ARNVNGTAILYGDRMTDSMKVAIRETERRREKQMAFNEANGITPRGVVKRIKDIIDGVYNADDVKAELTAAAERARYEDM
SEKQVAKEIKRLEKQMLDHAKNLEFEKAAQVRDQLAKLRSQVFGAGAEGDHLLPPSA

Sequences:

>Translated_697_residues
MTNLAEAAPALDEDKFVTFPGSPFQLYQPYSPAGDQPEAIRQLVEGVEDGLSYQTLLGVTGSGKTFTMANVIARMGRPAI
VFAPNKTLAAQLYSEFREFFPRNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQMRLSATKSLLERRDTIIVAT
VSAIYGIGNPSEYHQMILTLRTGDKISQRDVIARLIAMQYTRNETDFQRGTFRVRGDTLDIFPAEHAEMAVRVELFDDEV
DSLQFFDPLTGRVRQKIPRFTVYPSSHYVTPRETVLRAIEDIKVELRERLDFFYKENRLVEAQRLEQRTRFDLEMLTELG
FCKGIENYSRHLSGAKPGEPPPTLVDYLPPDALMFLDESHVLIGQLNGMYNGDRARKTTLVEYGFRLPSALDNRPLKFDE
YERKMRQAVFVTATPANYEKEHAGQVVEQVVRPTGLVDPVIEVRPATTQVDDLLGEIHQRVKVNERVLVTTLTKRMAEQL
TEFLSENGVKVRYLHSDIDTVERVEIIRDLRLGTFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRAERSLIQTIGRA
ARNVNGTAILYGDRMTDSMKVAIRETERRREKQMAFNEANGITPRGVVKRIKDIIDGVYNADDVKAELTAAAERARYEDM
SEKQVAKEIKRLEKQMLDHAKNLEFEKAAQVRDQLAKLRSQVFGAGAEGDHLLPPSA
>Mature_696_residues
TNLAEAAPALDEDKFVTFPGSPFQLYQPYSPAGDQPEAIRQLVEGVEDGLSYQTLLGVTGSGKTFTMANVIARMGRPAIV
FAPNKTLAAQLYSEFREFFPRNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQMRLSATKSLLERRDTIIVATV
SAIYGIGNPSEYHQMILTLRTGDKISQRDVIARLIAMQYTRNETDFQRGTFRVRGDTLDIFPAEHAEMAVRVELFDDEVD
SLQFFDPLTGRVRQKIPRFTVYPSSHYVTPRETVLRAIEDIKVELRERLDFFYKENRLVEAQRLEQRTRFDLEMLTELGF
CKGIENYSRHLSGAKPGEPPPTLVDYLPPDALMFLDESHVLIGQLNGMYNGDRARKTTLVEYGFRLPSALDNRPLKFDEY
ERKMRQAVFVTATPANYEKEHAGQVVEQVVRPTGLVDPVIEVRPATTQVDDLLGEIHQRVKVNERVLVTTLTKRMAEQLT
EFLSENGVKVRYLHSDIDTVERVEIIRDLRLGTFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRAERSLIQTIGRAA
RNVNGTAILYGDRMTDSMKVAIRETERRREKQMAFNEANGITPRGVVKRIKDIIDGVYNADDVKAELTAAAERARYEDMS
EKQVAKEIKRLEKQMLDHAKNLEFEKAAQVRDQLAKLRSQVFGAGAEGDHLLPPSA

Specific function: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA(2)B(2) complex to a putative damaged site, the DNA

COG id: COG0556

COG function: function code L; Helicase subunit of the DNA excision repair complex

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Escherichia coli, GI1786996, Length=670, Percent_Identity=67.1641791044776, Blast_Score=896, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=227, Percent_Identity=23.3480176211454, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR001943
- InterPro:   IPR004807
- InterPro:   IPR009055 [H]

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 79321; Mature: 79190

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLAEAAPALDEDKFVTFPGSPFQLYQPYSPAGDQPEAIRQLVEGVEDGLSYQTLLGVT
CCCHHHHCCCCCCCCEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEHHEEEC
GSGKTFTMANVIARMGRPAIVFAPNKTLAAQLYSEFREFFPRNAVEYFVSYYDYYQPEAY
CCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
VPQRDLFIEKDSSINEHIEQMRLSATKSLLERRDTIIVATVSAIYGIGNPSEYHQMILTL
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCCCHHHHHHEEEEE
RTGDKISQRDVIARLIAMQYTRNETDFQRGTFRVRGDTLDIFPAEHAEMAVRVELFDDEV
ECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEECCCCCCCEEEEEEEECCCH
DSLQFFDPLTGRVRQKIPRFTVYPSSHYVTPRETVLRAIEDIKVELRERLDFFYKENRLV
HHHHHHCHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
EAQRLEQRTRFDLEMLTELGFCKGIENYSRHLSGAKPGEPPPTLVDYLPPDALMFLDESH
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEECCCC
VLIGQLNGMYNGDRARKTTLVEYGFRLPSALDNRPLKFDEYERKMRQAVFVTATPANYEK
EEEEECCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHEEEEEECCCCCCH
EHAGQVVEQVVRPTGLVDPVIEVRPATTQVDDLLGEIHQRVKVNERVLVTTLTKRMAEQL
HHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TEFLSENGVKVRYLHSDIDTVERVEIIRDLRLGTFDVLVGINLLREGLDIPEVSLVAILD
HHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEE
ADKEGFLRAERSLIQTIGRAARNVNGTAILYGDRMTDSMKVAIRETERRREKQMAFNEAN
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GITPRGVVKRIKDIIDGVYNADDVKAELTAAAERARYEDMSEKQVAKEIKRLEKQMLDHA
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
KNLEFEKAAQVRDQLAKLRSQVFGAGAEGDHLLPPSA
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TNLAEAAPALDEDKFVTFPGSPFQLYQPYSPAGDQPEAIRQLVEGVEDGLSYQTLLGVT
CCHHHHCCCCCCCCEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEHHEEEC
GSGKTFTMANVIARMGRPAIVFAPNKTLAAQLYSEFREFFPRNAVEYFVSYYDYYQPEAY
CCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
VPQRDLFIEKDSSINEHIEQMRLSATKSLLERRDTIIVATVSAIYGIGNPSEYHQMILTL
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCCCHHHHHHEEEEE
RTGDKISQRDVIARLIAMQYTRNETDFQRGTFRVRGDTLDIFPAEHAEMAVRVELFDDEV
ECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEECCCCCCCEEEEEEEECCCH
DSLQFFDPLTGRVRQKIPRFTVYPSSHYVTPRETVLRAIEDIKVELRERLDFFYKENRLV
HHHHHHCHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
EAQRLEQRTRFDLEMLTELGFCKGIENYSRHLSGAKPGEPPPTLVDYLPPDALMFLDESH
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEECCCC
VLIGQLNGMYNGDRARKTTLVEYGFRLPSALDNRPLKFDEYERKMRQAVFVTATPANYEK
EEEEECCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHEEEEEECCCCCCH
EHAGQVVEQVVRPTGLVDPVIEVRPATTQVDDLLGEIHQRVKVNERVLVTTLTKRMAEQL
HHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TEFLSENGVKVRYLHSDIDTVERVEIIRDLRLGTFDVLVGINLLREGLDIPEVSLVAILD
HHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEE
ADKEGFLRAERSLIQTIGRAARNVNGTAILYGDRMTDSMKVAIRETERRREKQMAFNEAN
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GITPRGVVKRIKDIIDGVYNADDVKAELTAAAERARYEDMSEKQVAKEIKRLEKQMLDHA
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
KNLEFEKAAQVRDQLAKLRSQVFGAGAEGDHLLPPSA
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA