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The map label for this gene is rutE [C]

Identifier: 94309954

GI number: 94309954

Start: 1099347

End: 1099934

Strand: Direct

Name: rutE [C]

Synonym: Rmet_1009

Alternate gene names: 94309954

Gene position: 1099347-1099934 (Clockwise)

Preceding gene: 94309953

Following gene: 94309958

Centisome position: 27.99

GC content: 62.07

Gene sequence:

>588_bases
ATGAAATCCGTCGATCAGGCAGTCCTTGCACAATTGTTCACTGAAGCCCGCACCCACAACGTGTGGCAAGACAAGCATGT
GGATGATGCGCTGCTGAAGCAGATCTACGAAGCGATGAAATTCGGTCCGACCGCCGCAAATGGCTCGCCGGCACGCATCG
TGTTCGTGAAGTCGGCGGCCGAGAAGGCCCGTCTGGTCGAGTGCGTATCGGCTGGCAATGCTGACAAGACTCGCTCGGCG
CCGGTGACCGCGATCATCGCGTTTGACCGCAACTTCCACGACCAGCTGCCGAAGCTGTTCCCGCACGCCGATGCGCGTTC
GTGGTACGCCGGCAACGACGAGAAGATTGCCCGTGACGCGCTGATGAACAGCTCGCTGCAAGGTGGCTACTTCATCCTGG
CCGCCCGCGCACTGGGTCTGGACTGCGGCCCGATGGCGGGTTTCGATGCCGACAAGCTCAACGCCACGTTCTTCCCCGAC
GGCAAGTGGGCGGTCAACTTCCTGGTGAACCTGGGCTATGGCGAGGCCGACAAGCTGTTCCCGCGTGGTCCGCGCCTGAC
GTTCGACGAAGCCTGCCGCATCGTCTGA

Upstream 100 bases:

>100_bases
CGAACGGAATTGAACGGGTTCGTCAAAGCGCTTGTGGGCGCACGGCGGACGCTTGGGTACAATCGCGCTTTCTTTCATCA
CAAAACCCGGAAAACGCGCA

Downstream 100 bases:

>100_bases
CGCGCCGCTGACCCGAAACAAAAAGCGACCCATCGAGGTCGCTTTTTTGTTGGCGCGTGGACGGCTCAGGGGCGCACGGG
CTCAGGGGCTCAGGCCGACC

Product: malonic semialdehyde reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MKSVDQAVLAQLFTEARTHNVWQDKHVDDALLKQIYEAMKFGPTAANGSPARIVFVKSAAEKARLVECVSAGNADKTRSA
PVTAIIAFDRNFHDQLPKLFPHADARSWYAGNDEKIARDALMNSSLQGGYFILAARALGLDCGPMAGFDADKLNATFFPD
GKWAVNFLVNLGYGEADKLFPRGPRLTFDEACRIV

Sequences:

>Translated_195_residues
MKSVDQAVLAQLFTEARTHNVWQDKHVDDALLKQIYEAMKFGPTAANGSPARIVFVKSAAEKARLVECVSAGNADKTRSA
PVTAIIAFDRNFHDQLPKLFPHADARSWYAGNDEKIARDALMNSSLQGGYFILAARALGLDCGPMAGFDADKLNATFFPD
GKWAVNFLVNLGYGEADKLFPRGPRLTFDEACRIV
>Mature_195_residues
MKSVDQAVLAQLFTEARTHNVWQDKHVDDALLKQIYEAMKFGPTAANGSPARIVFVKSAAEKARLVECVSAGNADKTRSA
PVTAIIAFDRNFHDQLPKLFPHADARSWYAGNDEKIARDALMNSSLQGGYFILAARALGLDCGPMAGFDADKLNATFFPD
GKWAVNFLVNLGYGEADKLFPRGPRLTFDEACRIV

Specific function: Unknown

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family. HadB/RutE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787243, Length=194, Percent_Identity=50, Blast_Score=211, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 21380; Mature: 21380

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVDQAVLAQLFTEARTHNVWQDKHVDDALLKQIYEAMKFGPTAANGSPARIVFVKSAA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECHH
EKARLVECVSAGNADKTRSAPVTAIIAFDRNFHDQLPKLFPHADARSWYAGNDEKIARDA
HHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LMNSSLQGGYFILAARALGLDCGPMAGFDADKLNATFFPDGKWAVNFLVNLGYGEADKLF
HHCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEEEEECCCCCCCCCC
PRGPRLTFDEACRIV
CCCCCCCHHHHCCCC
>Mature Secondary Structure
MKSVDQAVLAQLFTEARTHNVWQDKHVDDALLKQIYEAMKFGPTAANGSPARIVFVKSAA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECHH
EKARLVECVSAGNADKTRSAPVTAIIAFDRNFHDQLPKLFPHADARSWYAGNDEKIARDA
HHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LMNSSLQGGYFILAARALGLDCGPMAGFDADKLNATFFPDGKWAVNFLVNLGYGEADKLF
HHCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEEEEECCCCCCCCCC
PRGPRLTFDEACRIV
CCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Fmn (Potential) [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]