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The map label for this gene is pldA [C]

Identifier: 94309951

GI number: 94309951

Start: 1096281

End: 1097690

Strand: Reverse

Name: pldA [C]

Synonym: Rmet_1006

Alternate gene names: 94309951

Gene position: 1097690-1096281 (Counterclockwise)

Preceding gene: 94309952

Following gene: 94309948

Centisome position: 27.94

GC content: 63.55

Gene sequence:

>1410_bases
ATGCCGCACGCGCCACACATGGTACGCTTGCGGGCATCGCAACTCTTGTCGCGTCCACGTCACCGGAGTGCTGTCGATAT
CCCGCCGGTGCGGCGCTTATTGCTCCCGATGCCACGTTACGTGTCAGGCCGTGTTGCACTTCCACGCACGGCCTTCCGCC
TGCTGTTGCCTACGCTGGCCTCGTCGGCCGTCCTGTTCCACGCCACGCCAGCCGATGCCGCAGTATCCGTGTTGCAGCCG
CCGCGTGTCATTGATGCCACTCAGCCCCTCACACTGACCCTGCTGATCACTGGGGATCGGGACATGCGGCGCTATGCCGT
ACCGGACTCCCTTGAGGTGTCGGCCTCTGGAGACCTTGTGGCACCGGCACCCATGACGCTCAAGCGCGTCGGCAAGGGGC
CAACCGTCCTGAACCTGCGGCAAGGCGAGCACCAGACCGTCAGCTTCCGCGGGGAATGGCCTACGACATTGCGTGGAGTG
GTCCGCCTGGACATCTCAGGGATCGACGCCGCGCCGGTCCTGATCACGCTGAACCAGGCGCCGGGCGCAAACCCGGCCAC
AGTTACTGCGCCAGTGGTCGCCGGCACGCCGGCCGAACCGAAAGTGGCCGTCGCGCCTGTCAGCGCTGGTCAGGTCACCC
CGGTCGTATCGGCCGAGAATGTCCCCGCATCCACCCCAGCGCCAGCCGATCTGCGCGACGCGCAACGGCTTTCCTTCAAC
GAGCCGATGTACATCATGTTCGGCGCGCACGACGGGGCCAACGCCAAGTTTCAGCTCAGCTTCAAGTTCCGGATCTACGA
GGGCAAGGATCCGGCGTCGAAGCGCTTCATCGACAACCTCTACTTCGCCTACACGCAGTTCTCGTTATGGGACCTGCACA
GCGCCTCGAAGCCGTTCGAGGACACCAACTATCGTCCGAGCCTGTATTACTACCTGTCCGACACGGGTGTGAAGAACAGC
GTGATCAGCCGGCTGTCGATCGCTTCGGGCTTCGAGCACGAGTCGAATGGCCGGGCCGGTGCCGACTCGCGCAGCATCAA
CACGCTGTTCCTCAAGCCGACGATGTACTTTGGCGACCAGAACGACTGGCACTGGCGCGTGGCGCCCAAAGTCTATGCGT
ACCTGGAGAAAAGCGAGAACGAGGACATCGCGCACTATCGTGGCTACATGGACCTGAACCTGGCCTACGGGAAGCCCGAC
TCGTGGGAATTCGCCGCCACGCTGCGCAAGGGCACGCGGCAGATGTACGGTAGCGTCGACGCGCAGGCCTCTTACCCGCT
TTCTCGCCTGATTCCGGGCACGGCTGGCTACCTGATGCTGGGCTACTTCTATGGCTATGGCGAAAGCCTGCTCAACTACA
ACCAGAAGACGCCCTGGCAGATCCGCCTGGGCTACGCGCTATCCCGCTAG

Upstream 100 bases:

>100_bases
GCCGGACGGGCAGGCCTACGTGGACGATATCCGACGCCGGGCCGCCGGCTGATCCGCACCCACCCGGTTTGGCCACGCCC
CGTGGCTAACCATTCGTCAC

Downstream 100 bases:

>100_bases
CGGTTTGCCGCTGGCCTTGAGCCGCGCATTGCCCACGGCCGCCGAGAACTGCCCATGGCCGTGCCAGCCCGTCGCGCGGC
CGAGCTTCTTGCCCTTGTGG

Product: putative phospholipase A

Products: NA

Alternate protein names: Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MPHAPHMVRLRASQLLSRPRHRSAVDIPPVRRLLLPMPRYVSGRVALPRTAFRLLLPTLASSAVLFHATPADAAVSVLQP
PRVIDATQPLTLTLLITGDRDMRRYAVPDSLEVSASGDLVAPAPMTLKRVGKGPTVLNLRQGEHQTVSFRGEWPTTLRGV
VRLDISGIDAAPVLITLNQAPGANPATVTAPVVAGTPAEPKVAVAPVSAGQVTPVVSAENVPASTPAPADLRDAQRLSFN
EPMYIMFGAHDGANAKFQLSFKFRIYEGKDPASKRFIDNLYFAYTQFSLWDLHSASKPFEDTNYRPSLYYYLSDTGVKNS
VISRLSIASGFEHESNGRAGADSRSINTLFLKPTMYFGDQNDWHWRVAPKVYAYLEKSENEDIAHYRGYMDLNLAYGKPD
SWEFAATLRKGTRQMYGSVDAQASYPLSRLIPGTAGYLMLGYFYGYGESLLNYNQKTPWQIRLGYALSR

Sequences:

>Translated_469_residues
MPHAPHMVRLRASQLLSRPRHRSAVDIPPVRRLLLPMPRYVSGRVALPRTAFRLLLPTLASSAVLFHATPADAAVSVLQP
PRVIDATQPLTLTLLITGDRDMRRYAVPDSLEVSASGDLVAPAPMTLKRVGKGPTVLNLRQGEHQTVSFRGEWPTTLRGV
VRLDISGIDAAPVLITLNQAPGANPATVTAPVVAGTPAEPKVAVAPVSAGQVTPVVSAENVPASTPAPADLRDAQRLSFN
EPMYIMFGAHDGANAKFQLSFKFRIYEGKDPASKRFIDNLYFAYTQFSLWDLHSASKPFEDTNYRPSLYYYLSDTGVKNS
VISRLSIASGFEHESNGRAGADSRSINTLFLKPTMYFGDQNDWHWRVAPKVYAYLEKSENEDIAHYRGYMDLNLAYGKPD
SWEFAATLRKGTRQMYGSVDAQASYPLSRLIPGTAGYLMLGYFYGYGESLLNYNQKTPWQIRLGYALSR
>Mature_468_residues
PHAPHMVRLRASQLLSRPRHRSAVDIPPVRRLLLPMPRYVSGRVALPRTAFRLLLPTLASSAVLFHATPADAAVSVLQPP
RVIDATQPLTLTLLITGDRDMRRYAVPDSLEVSASGDLVAPAPMTLKRVGKGPTVLNLRQGEHQTVSFRGEWPTTLRGVV
RLDISGIDAAPVLITLNQAPGANPATVTAPVVAGTPAEPKVAVAPVSAGQVTPVVSAENVPASTPAPADLRDAQRLSFNE
PMYIMFGAHDGANAKFQLSFKFRIYEGKDPASKRFIDNLYFAYTQFSLWDLHSASKPFEDTNYRPSLYYYLSDTGVKNSV
ISRLSIASGFEHESNGRAGADSRSINTLFLKPTMYFGDQNDWHWRVAPKVYAYLEKSENEDIAHYRGYMDLNLAYGKPDS
WEFAATLRKGTRQMYGSVDAQASYPLSRLIPGTAGYLMLGYFYGYGESLLNYNQKTPWQIRLGYALSR

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=213, Percent_Identity=31.924882629108, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 51794; Mature: 51662

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHAPHMVRLRASQLLSRPRHRSAVDIPPVRRLLLPMPRYVSGRVALPRTAFRLLLPTLA
CCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHCCCEECCHHHHHHHHHHHC
SSAVLFHATPADAAVSVLQPPRVIDATQPLTLTLLITGDRDMRRYAVPDSLEVSASGDLV
CCEEEEEECCHHHHHHHCCCCCEECCCCCEEEEEEEECCCCCEEECCCCCEEEECCCCEE
APAPMTLKRVGKGPTVLNLRQGEHQTVSFRGEWPTTLRGVVRLDISGIDAAPVLITLNQA
CCCCHHHHHCCCCCCEEEEECCCCEEEEEECCCCHHEEEEEEEEECCCCCCEEEEEEECC
PGANPATVTAPVVAGTPAEPKVAVAPVSAGQVTPVVSAENVPASTPAPADLRDAQRLSFN
CCCCCCEEEEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCC
EPMYIMFGAHDGANAKFQLSFKFRIYEGKDPASKRFIDNLYFAYTQFSLWDLHSASKPFE
CCEEEEEECCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHEEEEEEEEEECCCCCCCCC
DTNYRPSLYYYLSDTGVKNSVISRLSIASGFEHESNGRAGADSRSINTLFLKPTMYFGDQ
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCC
NDWHWRVAPKVYAYLEKSENEDIAHYRGYMDLNLAYGKPDSWEFAATLRKGTRQMYGSVD
CCEEEEECHHEEEEECCCCCCCHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
AQASYPLSRLIPGTAGYLMLGYFYGYGESLLNYNQKTPWQIRLGYALSR
CCCCCCHHHHCCCCCHHHHHHHHHHHCHHHHCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
PHAPHMVRLRASQLLSRPRHRSAVDIPPVRRLLLPMPRYVSGRVALPRTAFRLLLPTLA
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHCCCEECCHHHHHHHHHHHC
SSAVLFHATPADAAVSVLQPPRVIDATQPLTLTLLITGDRDMRRYAVPDSLEVSASGDLV
CCEEEEEECCHHHHHHHCCCCCEECCCCCEEEEEEEECCCCCEEECCCCCEEEECCCCEE
APAPMTLKRVGKGPTVLNLRQGEHQTVSFRGEWPTTLRGVVRLDISGIDAAPVLITLNQA
CCCCHHHHHCCCCCCEEEEECCCCEEEEEECCCCHHEEEEEEEEECCCCCCEEEEEEECC
PGANPATVTAPVVAGTPAEPKVAVAPVSAGQVTPVVSAENVPASTPAPADLRDAQRLSFN
CCCCCCEEEEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCC
EPMYIMFGAHDGANAKFQLSFKFRIYEGKDPASKRFIDNLYFAYTQFSLWDLHSASKPFE
CCEEEEEECCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHEEEEEEEEEECCCCCCCCC
DTNYRPSLYYYLSDTGVKNSVISRLSIASGFEHESNGRAGADSRSINTLFLKPTMYFGDQ
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCC
NDWHWRVAPKVYAYLEKSENEDIAHYRGYMDLNLAYGKPDSWEFAATLRKGTRQMYGSVD
CCEEEEECHHEEEEECCCCCCCHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
AQASYPLSRLIPGTAGYLMLGYFYGYGESLLNYNQKTPWQIRLGYALSR
CCCCCCHHHHCCCCCHHHHHHHHHHHCHHHHCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]