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The map label for this gene is gcvA [H]

Identifier: 94309928

GI number: 94309928

Start: 1068916

End: 1069818

Strand: Direct

Name: gcvA [H]

Synonym: Rmet_0983

Alternate gene names: 94309928

Gene position: 1068916-1069818 (Clockwise)

Preceding gene: 94309924

Following gene: 94309932

Centisome position: 27.21

GC content: 65.89

Gene sequence:

>903_bases
ATGCGACGCTTATGCCCGTCGCTGACGGAATTGCAGGCATTCGAAGCCGCTGCGCGGCACAACAGCTTCACGCTGGCCGC
CCGGGAGCTCCACGTCACGCAGGGGGCGGTCAGCAAGCAGGTGCGCAGTCTCGAGGCGTTTCTGGGTGTCGAACTGTTCG
AGCGCGTGCGCCAGCGTCTGATGCTGACCGGCGCGGGGGAGCGCTATCTGGAGCGTATCCGGCCGAGCCTGAACGAGATC
GAGGCGGCCAGCGTGGAGTTGATCGCGCGCAAGGGGCGGGGCGGCATCCTGCATATCGCCAGCATGCCGACGCTCGGCGC
CAAGTGGCTGATTCCGCGGCTGCCGCAGTTTTTCGCCCGGCATCCCGAAGTCACGCTGGAGTTCGTTCCCCATGCCCAGG
GCTACGATTTTTCCCAACCTGATCTCGATGCGGCGATCCGCTTCGGCGACGGCGTCTGGCCGGGAAGCCTCGCCGACTAT
ATGACTGGACGCGAAGTGCTGCCGGTGTGCCGGCCGGGATTGCTCGCGCAGGAGTCTGGGGGCGCGGTTGCATCAGCCGA
GGGTTTGCTGCGTTATCCGCTGCTGCATCACACCACGGTGCCGGAGGCGTGGCCAGACTGGCTGTCCGAACTGGGGGTGT
CCACGCGCCAGGCATGGAGCGGGGCACGTTTCGATCAGTTCTCGTTGCTGACACAGGCGGCGGTGTCGGGGCTAGGCATC
GCGCTGATTCCGCGCTGCCTGATCGAAGAGGAGATTGCCACCGGCACGCTGGTGGTGGCGCATGCCGCGCGCGTGCTGGC
CAAGAAGGGGTATTACCTCTGCTATCCGGAACAGAAGGCTCACCTGCCGGCGCTGCAGACGTTCCGGGCTTGGTTGATGG
AAGGTGTCGACGTGGTGGCGTAG

Upstream 100 bases:

>100_bases
AATTCTTTGGCTCTCATGATTCTGCTTGCCGATGCAAGATGCGTGCAAACGAAATAAAATCCCCAGGTCATTCCAAATGG
TATTGAATTAAAGGTGCTCT

Downstream 100 bases:

>100_bases
CGGCCCGGCAAGCGCTCATTCCAGGCGCTAACTCCGGGCGCTACGTCATCCACTATTTCGACAGTACTTTCATCGCTGCC
TCGAGTCCCGCGATCGTGAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRRLCPSLTELQAFEAAARHNSFTLAARELHVTQGAVSKQVRSLEAFLGVELFERVRQRLMLTGAGERYLERIRPSLNEI
EAASVELIARKGRGGILHIASMPTLGAKWLIPRLPQFFARHPEVTLEFVPHAQGYDFSQPDLDAAIRFGDGVWPGSLADY
MTGREVLPVCRPGLLAQESGGAVASAEGLLRYPLLHHTTVPEAWPDWLSELGVSTRQAWSGARFDQFSLLTQAAVSGLGI
ALIPRCLIEEEIATGTLVVAHAARVLAKKGYYLCYPEQKAHLPALQTFRAWLMEGVDVVA

Sequences:

>Translated_300_residues
MRRLCPSLTELQAFEAAARHNSFTLAARELHVTQGAVSKQVRSLEAFLGVELFERVRQRLMLTGAGERYLERIRPSLNEI
EAASVELIARKGRGGILHIASMPTLGAKWLIPRLPQFFARHPEVTLEFVPHAQGYDFSQPDLDAAIRFGDGVWPGSLADY
MTGREVLPVCRPGLLAQESGGAVASAEGLLRYPLLHHTTVPEAWPDWLSELGVSTRQAWSGARFDQFSLLTQAAVSGLGI
ALIPRCLIEEEIATGTLVVAHAARVLAKKGYYLCYPEQKAHLPALQTFRAWLMEGVDVVA
>Mature_300_residues
MRRLCPSLTELQAFEAAARHNSFTLAARELHVTQGAVSKQVRSLEAFLGVELFERVRQRLMLTGAGERYLERIRPSLNEI
EAASVELIARKGRGGILHIASMPTLGAKWLIPRLPQFFARHPEVTLEFVPHAQGYDFSQPDLDAAIRFGDGVWPGSLADY
MTGREVLPVCRPGLLAQESGGAVASAEGLLRYPLLHHTTVPEAWPDWLSELGVSTRQAWSGARFDQFSLLTQAAVSGLGI
ALIPRCLIEEEIATGTLVVAHAARVLAKKGYYLCYPEQKAHLPALQTFRAWLMEGVDVVA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=34.4709897610922, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=31.0580204778157, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788706, Length=299, Percent_Identity=27.4247491638796, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1786401, Length=301, Percent_Identity=29.2358803986711, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787589, Length=305, Percent_Identity=26.2295081967213, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=144, Percent_Identity=36.8055555555556, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=26.1168384879725, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787128, Length=299, Percent_Identity=23.7458193979933, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789204, Length=128, Percent_Identity=37.5, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081978, Length=256, Percent_Identity=27.34375, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790399, Length=119, Percent_Identity=35.2941176470588, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789639, Length=253, Percent_Identity=26.4822134387352, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788887, Length=124, Percent_Identity=36.2903225806452, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787806, Length=247, Percent_Identity=25.1012145748988, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33032; Mature: 33032

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLCPSLTELQAFEAAARHNSFTLAARELHVTQGAVSKQVRSLEAFLGVELFERVRQRL
CCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLTGAGERYLERIRPSLNEIEAASVELIARKGRGGILHIASMPTLGAKWLIPRLPQFFAR
HHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHC
HPEVTLEFVPHAQGYDFSQPDLDAAIRFGDGVWPGSLADYMTGREVLPVCRPGLLAQESG
CCCEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCCHHHCCCCCEEECCC
GAVASAEGLLRYPLLHHTTVPEAWPDWLSELGVSTRQAWSGARFDQFSLLTQAAVSGLGI
CCEECCCCHHHCCHHHCCCCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCH
ALIPRCLIEEEIATGTLVVAHAARVLAKKGYYLCYPEQKAHLPALQTFRAWLMEGVDVVA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCHHHCC
>Mature Secondary Structure
MRRLCPSLTELQAFEAAARHNSFTLAARELHVTQGAVSKQVRSLEAFLGVELFERVRQRL
CCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLTGAGERYLERIRPSLNEIEAASVELIARKGRGGILHIASMPTLGAKWLIPRLPQFFAR
HHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHC
HPEVTLEFVPHAQGYDFSQPDLDAAIRFGDGVWPGSLADYMTGREVLPVCRPGLLAQESG
CCCEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCCHHHCCCCCEEECCC
GAVASAEGLLRYPLLHHTTVPEAWPDWLSELGVSTRQAWSGARFDQFSLLTQAAVSGLGI
CCEECCCCHHHCCHHHCCCCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCH
ALIPRCLIEEEIATGTLVVAHAARVLAKKGYYLCYPEQKAHLPALQTFRAWLMEGVDVVA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]