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The map label for this gene is ureF

Identifier: 94309908

GI number: 94309908

Start: 1048074

End: 1048766

Strand: Direct

Name: ureF

Synonym: Rmet_0963

Alternate gene names: 94309908

Gene position: 1048074-1048766 (Clockwise)

Preceding gene: 94309907

Following gene: 94309909

Centisome position: 26.68

GC content: 65.66

Gene sequence:

>693_bases
ATGACCGCGCTCCCCCAGCTCATCTCCCTGCTGCACCTTGCCTCGCCGGCGCTCCCCATCGGCGGCTTCAGCTATTCGCA
GGGACTGGAGGCGGCCATCGATTGCGAGTTGGTGCGGGATGCGACGACTGCCGAACGCTGGATTCGCGACAACCTCCTGC
ATGTGCAGGCCCAATGCGAGGCGCCTGTCTGGCTACTGCTGCATCGCGCGTGGCAGACGCAGGACCACGCCTCCGTGCGG
CAATGGAACGACTGGTTTCATGCCACCCGGGAAACATCCGAACTGCGGCTCGAGACCGAGCAGATGGGCTGGTCGCTGGC
GAAACTGATCGCGCAGATGGGTTGGGGCGACGATGCGTCCCGCGACCTGCTGCGCGACATGCGACCGGTCTGCCTGCCCA
CGGCGTTTTCCTCGGCCTGCGTGGCGCTAGGTATCGCTGCGCGTGAAGGCTTGGCCGCGTATCTCTTCAACTGGGCCGAA
AACCAGGTGGCAGCCGCCATCAAGGCGGTGCCGCTCGGCCAGGTGGCGGGCCAGCAGATGCTGCTTGGCTTGCATCAGGC
GGTGCTCGGCACCGTCGATGAAGCAGTGTGCCGCGCCGATGCGGTGCCACCATTGCTTTCCACGTTCTCCCCGATGCTAG
GCGTACTGTCGGCGCGTCACGAGACGCAGTACTCCCGGCTGTTCCGATCCTGA

Upstream 100 bases:

>100_bases
ACATGACCATGACCATGACCATGACCATGATCACGTGCATGACGAGCATTGCGGTCACAAACATTGAGTTGCGCCTGACA
GCGTCAGCAGAGCCATCCCC

Downstream 100 bases:

>100_bases
GATCCGATCATGACTCGTACCAAGAAAAACCCTCCGTTGCGCGTTGGCGTGGGCGGCCCCGTCGGCTCCGGCAAGACCAC
GCTGCTCGAGATGCTGTGCA

Product: Urea amidohydrolase (urease) accessory protein UreF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTALPQLISLLHLASPALPIGGFSYSQGLEAAIDCELVRDATTAERWIRDNLLHVQAQCEAPVWLLLHRAWQTQDHASVR
QWNDWFHATRETSELRLETEQMGWSLAKLIAQMGWGDDASRDLLRDMRPVCLPTAFSSACVALGIAAREGLAAYLFNWAE
NQVAAAIKAVPLGQVAGQQMLLGLHQAVLGTVDEAVCRADAVPPLLSTFSPMLGVLSARHETQYSRLFRS

Sequences:

>Translated_230_residues
MTALPQLISLLHLASPALPIGGFSYSQGLEAAIDCELVRDATTAERWIRDNLLHVQAQCEAPVWLLLHRAWQTQDHASVR
QWNDWFHATRETSELRLETEQMGWSLAKLIAQMGWGDDASRDLLRDMRPVCLPTAFSSACVALGIAAREGLAAYLFNWAE
NQVAAAIKAVPLGQVAGQQMLLGLHQAVLGTVDEAVCRADAVPPLLSTFSPMLGVLSARHETQYSRLFRS
>Mature_229_residues
TALPQLISLLHLASPALPIGGFSYSQGLEAAIDCELVRDATTAERWIRDNLLHVQAQCEAPVWLLLHRAWQTQDHASVRQ
WNDWFHATRETSELRLETEQMGWSLAKLIAQMGWGDDASRDLLRDMRPVCLPTAFSSACVALGIAAREGLAAYLFNWAEN
QVAAAIKAVPLGQVAGQQMLLGLHQAVLGTVDEAVCRADAVPPLLSTFSPMLGVLSARHETQYSRLFRS

Specific function: Required for maturation of urease via the functional incorporation of the urease nickel metallocenter

COG id: COG0830

COG function: function code O; Urease accessory protein UreF

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ureF family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UREF_RALME (Q1LPS7)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_583118.1
- STRING:   Q1LPS7
- GeneID:   4037760
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0963
- eggNOG:   COG0830
- HOGENOM:   HBG709295
- OMA:   SPQLPIG
- ProtClustDB:   CLSK897184
- BioCyc:   RMET266264:RMET_0963-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01385
- InterPro:   IPR002639
- PIRSF:   PIRSF009467

Pfam domain/function: PF01730 UreF

EC number: NA

Molecular weight: Translated: 25327; Mature: 25196

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALPQLISLLHLASPALPIGGFSYSQGLEAAIDCELVRDATTAERWIRDNLLHVQAQCE
CCCHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
APVWLLLHRAWQTQDHASVRQWNDWFHATRETSELRLETEQMGWSLAKLIAQMGWGDDAS
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
RDLLRDMRPVCLPTAFSSACVALGIAAREGLAAYLFNWAENQVAAAIKAVPLGQVAGQQM
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LLGLHQAVLGTVDEAVCRADAVPPLLSTFSPMLGVLSARHETQYSRLFRS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TALPQLISLLHLASPALPIGGFSYSQGLEAAIDCELVRDATTAERWIRDNLLHVQAQCE
CCHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
APVWLLLHRAWQTQDHASVRQWNDWFHATRETSELRLETEQMGWSLAKLIAQMGWGDDAS
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
RDLLRDMRPVCLPTAFSSACVALGIAAREGLAAYLFNWAENQVAAAIKAVPLGQVAGQQM
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LLGLHQAVLGTVDEAVCRADAVPPLLSTFSPMLGVLSARHETQYSRLFRS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA