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The map label for this gene is pnp [H]

Identifier: 94309868

GI number: 94309868

Start: 1007462

End: 1009639

Strand: Direct

Name: pnp [H]

Synonym: Rmet_0923

Alternate gene names: 94309868

Gene position: 1007462-1009639 (Clockwise)

Preceding gene: 94309866

Following gene: 94309869

Centisome position: 25.65

GC content: 63.87

Gene sequence:

>2178_bases
ATGACCATGTTCAACAAGATCGTCAAGGAATTCCAGTGGGGCCAGCACACGGTCCGCATGGAAACCGGCGAAATCGCGCG
CCAGGCATCTGGCGCCGTGATCGTCGACGTGGAAGACACCGTGGTGCTGGCCACCGTGGTGGCAGCCAAGAGCCCGAAGG
CGGGTCAGGACTTCTTCCCGCTGACCGTCGACTACATCGAGAAGACCTACGCTGCTGGCAAGATCCCCGGTGGCTTCTTC
AAGCGTGAAGGCCGTCCGTCGGAAAACGAGACGCTGACCTCGCGCCTGATCGACCGTCCGCTGCGTCCGCTGTTCCCGGA
AGGTTTCTACAACGACGTGCAGGTGGTGATCCACGTGCTGTCGATCAACCCGGAAATCCCGGCTGACATCCCCGCGCTGA
TCGCTTCGTCGGCTGCGCTGGCCGTTTCGGGTATCCCGTTCAACGGTCCGGTTGGCGCTGCCCGCGTCGGCTACAAGGAC
GGCCAGTACCTGCTGAACCCGACCCGCGCGCAAATCGCCGCGTCGGATCTGGACCTGGTGGTCGCCGGTACCGAACGTGC
CGTGCTGATGGTGGAATCGGAAGCCCACCAACTGTCGGAAGACGTGATGCTCGGCGCCGTGGTGTACGGTCACGAGCAAA
TGCAGATCGCGATCAACGCAATCCACGACCTGGTGCGCGAAGGCGGCAAGCCCGAGTGGGACTGGGCTCCGGCTCCGAAG
AATGAAGCGCTGATCGCCAAGGTTAGCGAGATTGGCCTGCCGCTGCTGCAGCAAGCCTACCAACTGCGTCAGAAGTCGGC
GCGCAGCACCAAGCTCAAGGAAATCTACGCAACGGTGCAGGCCCAACTGGCCGAGGCCGGCGTGGAAGCCGACAAGGTGG
AAGTGGGCAACGTCCTGTTCGACCTCGAGGCGAAGATCGTGCGCGGCCAGATCCTGGCTGGCGAGCCGCGTATCGACGGC
CGCGACACGCGCACCGTGCGCCCGATCGAAATCCGTTCGTCGGTGCTGCCGCGCGCGCACGGCTCGGCGCTGTTCACCCG
TGGTGAAACGCAGGCACTCGTGGTGGCCACGCTCGGCACCAAGAGCGATGAGCAGATCATCGACGCACTGGCCGGCGAGT
ACCGCGATCGCTTCATGCTCCACTACAACATGCCTCCGTTCGCCACCGGCGAAACGGGTCGCGTTGGTAGCCCGAAGCGT
CGTGAAATCGGTCACGGCCGTCTGGCCAAGCGCGCGCTGATCCCGGTGCTGCCGAAGGACGACGAGTTCGCGTACACGAT
CCGTCTGGTGTCGGAAATCACCGAATCGAACGGTTCGTCGTCGATGGCTTCGGTCTGCGGCGGCTGCCTGGCGCTGATGG
ATGCTGGCGTTCCGGTGAAGGCGCACGTGGCCGGCGTGGCCATGGGCCTGATCCTGGAGGGCAACAAGTTTGCCGTGCTG
ACCGACATCCTCGGTGACGAGGATCACCTCGGCGACATGGACTTCAAGGTGGCCGGTACCGACGCGGGCATTACCGCGCT
GCAGATGGACATCAAGGTCCAGGGCATCACCAAGGAAATCATGCAGGTGGCGCTGGCCCAGGCCCGCGAAGGCCGCATGC
ACATCCTCGGCGCGATGCAAGGCGCAATGGGTCACGCTCGCACCGAACTGTCGGCGCACGCTCCGCGCATGATCACGATG
AAGATCCATCCGGACAAGATCCGCGAAGTGATCGGCAAGGGCGGCTCGACGATCCAAGCACTGACCAAGGAAACCGGCAC
GACCATCGACATCCAGGAAGACGGCACGATCACGATCGCGTCGACGTCGACCGACGGCATGGCCGAAGCCAAGCGCCGCA
TCGAAGGCATCACCGCGGAAGCCGAAGTGGGCAAGATCTACGCCGGTACCGTGCTGAAGCTGCTGGACTTTGGCGCCATC
GTCAACATCCTGCCGGGCAAGGACGGTCTGCTGCATATCTCCGAGATCGTCAACGAGCGCGTCAAGGACATCAAGGACTG
GCTCAAGGAAGGCCAACAGGTTCGCGTCAAGCTGATCCAGGCCGATGAAAAGGGTCGTCTGCGTCTGTCCCTGAAGGCAG
CGCTGGCCGAAGAGGGCGGCAGCATCAGCCCGATCGCGCAAGGCGATGCACCGGCCGCGGCACCTGCCGCCCCGGCATCG
CCCGATCAGCAGCAGTAA

Upstream 100 bases:

>100_bases
GCGGACCGGATTACCTCCGGCAGTTCGCCTGCCGCTGCGAGCGCAGGCGTCGCCGATCGTGCATGTGTGCGGCCGGCAAG
CATGAAATCAAGGAACGCTC

Downstream 100 bases:

>100_bases
GCTGACGGCGAACGCGGTCACCGCGTAGCACCCATGAAAGCGGCTGGCGATGTCCAGCCGCTTTTTTGTTTACACTGCCG
GTCAGTTCGCTATCCGGTTC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 725; Mature: 724

Protein sequence:

>725_residues
MTMFNKIVKEFQWGQHTVRMETGEIARQASGAVIVDVEDTVVLATVVAAKSPKAGQDFFPLTVDYIEKTYAAGKIPGGFF
KREGRPSENETLTSRLIDRPLRPLFPEGFYNDVQVVIHVLSINPEIPADIPALIASSAALAVSGIPFNGPVGAARVGYKD
GQYLLNPTRAQIAASDLDLVVAGTERAVLMVESEAHQLSEDVMLGAVVYGHEQMQIAINAIHDLVREGGKPEWDWAPAPK
NEALIAKVSEIGLPLLQQAYQLRQKSARSTKLKEIYATVQAQLAEAGVEADKVEVGNVLFDLEAKIVRGQILAGEPRIDG
RDTRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGTKSDEQIIDALAGEYRDRFMLHYNMPPFATGETGRVGSPKR
REIGHGRLAKRALIPVLPKDDEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVL
TDILGDEDHLGDMDFKVAGTDAGITALQMDIKVQGITKEIMQVALAQAREGRMHILGAMQGAMGHARTELSAHAPRMITM
KIHPDKIREVIGKGGSTIQALTKETGTTIDIQEDGTITIASTSTDGMAEAKRRIEGITAEAEVGKIYAGTVLKLLDFGAI
VNILPGKDGLLHISEIVNERVKDIKDWLKEGQQVRVKLIQADEKGRLRLSLKAALAEEGGSISPIAQGDAPAAAPAAPAS
PDQQQ

Sequences:

>Translated_725_residues
MTMFNKIVKEFQWGQHTVRMETGEIARQASGAVIVDVEDTVVLATVVAAKSPKAGQDFFPLTVDYIEKTYAAGKIPGGFF
KREGRPSENETLTSRLIDRPLRPLFPEGFYNDVQVVIHVLSINPEIPADIPALIASSAALAVSGIPFNGPVGAARVGYKD
GQYLLNPTRAQIAASDLDLVVAGTERAVLMVESEAHQLSEDVMLGAVVYGHEQMQIAINAIHDLVREGGKPEWDWAPAPK
NEALIAKVSEIGLPLLQQAYQLRQKSARSTKLKEIYATVQAQLAEAGVEADKVEVGNVLFDLEAKIVRGQILAGEPRIDG
RDTRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGTKSDEQIIDALAGEYRDRFMLHYNMPPFATGETGRVGSPKR
REIGHGRLAKRALIPVLPKDDEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVL
TDILGDEDHLGDMDFKVAGTDAGITALQMDIKVQGITKEIMQVALAQAREGRMHILGAMQGAMGHARTELSAHAPRMITM
KIHPDKIREVIGKGGSTIQALTKETGTTIDIQEDGTITIASTSTDGMAEAKRRIEGITAEAEVGKIYAGTVLKLLDFGAI
VNILPGKDGLLHISEIVNERVKDIKDWLKEGQQVRVKLIQADEKGRLRLSLKAALAEEGGSISPIAQGDAPAAAPAAPAS
PDQQQ
>Mature_724_residues
TMFNKIVKEFQWGQHTVRMETGEIARQASGAVIVDVEDTVVLATVVAAKSPKAGQDFFPLTVDYIEKTYAAGKIPGGFFK
REGRPSENETLTSRLIDRPLRPLFPEGFYNDVQVVIHVLSINPEIPADIPALIASSAALAVSGIPFNGPVGAARVGYKDG
QYLLNPTRAQIAASDLDLVVAGTERAVLMVESEAHQLSEDVMLGAVVYGHEQMQIAINAIHDLVREGGKPEWDWAPAPKN
EALIAKVSEIGLPLLQQAYQLRQKSARSTKLKEIYATVQAQLAEAGVEADKVEVGNVLFDLEAKIVRGQILAGEPRIDGR
DTRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGTKSDEQIIDALAGEYRDRFMLHYNMPPFATGETGRVGSPKRR
EIGHGRLAKRALIPVLPKDDEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVLT
DILGDEDHLGDMDFKVAGTDAGITALQMDIKVQGITKEIMQVALAQAREGRMHILGAMQGAMGHARTELSAHAPRMITMK
IHPDKIREVIGKGGSTIQALTKETGTTIDIQEDGTITIASTSTDGMAEAKRRIEGITAEAEVGKIYAGTVLKLLDFGAIV
NILPGKDGLLHISEIVNERVKDIKDWLKEGQQVRVKLIQADEKGRLRLSLKAALAEEGGSISPIAQGDAPAAAPAAPASP
DQQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=704, Percent_Identity=38.4943181818182, Blast_Score=453, Evalue=1e-127,
Organism=Escherichia coli, GI145693187, Length=692, Percent_Identity=61.4161849710983, Blast_Score=884, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=707, Percent_Identity=32.956152758133, Blast_Score=323, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17535281, Length=104, Percent_Identity=37.5, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281362905, Length=708, Percent_Identity=37.0056497175141, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651641, Length=708, Percent_Identity=37.0056497175141, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651643, Length=708, Percent_Identity=37.0056497175141, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI161079377, Length=649, Percent_Identity=36.8258859784284, Blast_Score=387, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77833; Mature: 77702

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMFNKIVKEFQWGQHTVRMETGEIARQASGAVIVDVEDTVVLATVVAAKSPKAGQDFFP
CCHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEHHHHCCCCCCCCCCCC
LTVDYIEKTYAAGKIPGGFFKREGRPSENETLTSRLIDRPLRPLFPEGFYNDVQVVIHVL
CHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHEEHE
SINPEIPADIPALIASSAALAVSGIPFNGPVGAARVGYKDGQYLLNPTRAQIAASDLDLV
ECCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEECCHHHHHHHCCCEEE
VAGTERAVLMVESEAHQLSEDVMLGAVVYGHEQMQIAINAIHDLVREGGKPEWDWAPAPK
EECCCEEEEEEECHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NEALIAKVSEIGLPLLQQAYQLRQKSARSTKLKEIYATVQAQLAEAGVEADKVEVGNVLF
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCEEE
DLEAKIVRGQILAGEPRIDGRDTRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGT
EEHHEEEECEEEECCCCCCCCCCCEECCCHHHHHHCCCCCCCEEEECCCCCEEEEEEECC
KSDEQIIDALAGEYRDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALIPVLPKD
CCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCCCCCC
DEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVL
CCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECCCEEEE
TDILGDEDHLGDMDFKVAGTDAGITALQMDIKVQGITKEIMQVALAQAREGRMHILGAMQ
EECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEHHHH
GAMGHARTELSAHAPRMITMKIHPDKIREVIGKGGSTIQALTKETGTTIDIQEDGTITIA
HHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCEEEEE
STSTDGMAEAKRRIEGITAEAEVGKIYAGTVLKLLDFGAIVNILPGKDGLLHISEIVNER
ECCCCHHHHHHHHHHCCCCCHHCCEEHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
VKDIKDWLKEGQQVRVKLIQADEKGRLRLSLKAALAEEGGSISPIAQGDAPAAAPAAPAS
HHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCC
PDQQQ
CCCCC
>Mature Secondary Structure 
TMFNKIVKEFQWGQHTVRMETGEIARQASGAVIVDVEDTVVLATVVAAKSPKAGQDFFP
CHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEHHHHCCCCCCCCCCCC
LTVDYIEKTYAAGKIPGGFFKREGRPSENETLTSRLIDRPLRPLFPEGFYNDVQVVIHVL
CHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHEEHE
SINPEIPADIPALIASSAALAVSGIPFNGPVGAARVGYKDGQYLLNPTRAQIAASDLDLV
ECCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEECCHHHHHHHCCCEEE
VAGTERAVLMVESEAHQLSEDVMLGAVVYGHEQMQIAINAIHDLVREGGKPEWDWAPAPK
EECCCEEEEEEECHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NEALIAKVSEIGLPLLQQAYQLRQKSARSTKLKEIYATVQAQLAEAGVEADKVEVGNVLF
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCEEE
DLEAKIVRGQILAGEPRIDGRDTRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGT
EEHHEEEECEEEECCCCCCCCCCCEECCCHHHHHHCCCCCCCEEEECCCCCEEEEEEECC
KSDEQIIDALAGEYRDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALIPVLPKD
CCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCCCCCC
DEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVL
CCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECCCEEEE
TDILGDEDHLGDMDFKVAGTDAGITALQMDIKVQGITKEIMQVALAQAREGRMHILGAMQ
EECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEHHHH
GAMGHARTELSAHAPRMITMKIHPDKIREVIGKGGSTIQALTKETGTTIDIQEDGTITIA
HHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCEEEEE
STSTDGMAEAKRRIEGITAEAEVGKIYAGTVLKLLDFGAIVNILPGKDGLLHISEIVNER
ECCCCHHHHHHHHHHCCCCCHHCCEEHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
VKDIKDWLKEGQQVRVKLIQADEKGRLRLSLKAALAEEGGSISPIAQGDAPAAAPAAPAS
HHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCC
PDQQQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA