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The map label for this gene is desA [H]

Identifier: 94309833

GI number: 94309833

Start: 972207

End: 973388

Strand: Reverse

Name: desA [H]

Synonym: Rmet_0888

Alternate gene names: 94309833

Gene position: 973388-972207 (Counterclockwise)

Preceding gene: 94309834

Following gene: 94309832

Centisome position: 24.78

GC content: 64.38

Gene sequence:

>1182_bases
ATGACTGCCCCATCTGACACCCTCTCGCCGGCCGACGCCGACGCGATCTCGCAAGCGCCGAATCACGGCACGAACCGCCC
CAAACGCCCGGCCAAGAACATTCCCCTGCCGGAGCCAATCGCCTTCGAGGCCCCATTTCCGAATCGCAAGACGATCCGCA
GCTGGCTGATTCCCCTCGGCGGCCGCAGCACCGCGCGCGCGCTGCTTCTGTTCGCGTTCGATTACGCGCTGTTCGCTGCC
GTACTGGCCGGTGTGGTCCTGGCATCACCCTGGTGGGCCAAGGTTGGGCTCGGCGTGCTGGCCGGACTGATCATCGCGCG
CCTGTTCATCATTGGGCACGATGCCTGCCACCAGAGCCTGACCGACCGTCGTGGCCTGAACAAGTGGCTGGGCCGGCTCA
CGTTCCTGCCGTCGCTCACGCCTTACAGCCTCTGGGAAGTCGGCCATAACGTCGTCCATCATGGTTACACGAACCTGAAG
GGCTTCGACTTCGTCTGGGCGCCCTACTCGCTCGAGGAATTCCGCGCGCTGCCGCGCAGCCGCCGGATTCTGGAGCGCAT
CTACCGCAACGGCCTTGGCGCCGGCCTTTACTACCTGATCGAGATCTGGTGGTTCAAGCTGTTCTTCCCCAGCAAGCGCC
AGATGGCGACGCGTCGCCCGATCTTCATGTGGGACTGCGCGCTCGTGGGAGCTTTCGGCGCAGTCTGGATCGGCGCGCTG
GTATGGCTGGCCGTGGCCACCGGGCAATCCCCGCTGCTGCTGGTCGGCACCGGCTTCGTGCTGCCCTTCCTGATCTGGAA
CGCCACCGTTGGCTTCGTGCTGTACGTCCACCATACGCATACCAGCGTGGCGTGGTACGACACCAAGGCCATGTGGGCGA
AGGCCCAGCCTTTCGTGTCGACGACCGTGCATCTGCGCTTCAACTTCGGCATCGGCGCGGCCCTGCATCACATCATGGAG
CACACGGCCCACCACGTGGACATGAGCATCCCGCTGTACCAGCTCAAGCGCGCCCAGGCGCTGCTCGAGCACGCACTGCC
GGGTCGCATCATCATCGAGAAGTTCTCGTGGCGCTGGTATTTCCATACAGCCCACCGCTGCAAGCTCTACGACTTCAAGG
CGCTGTGCTGGACGGATTTCCGCGGTCGCCAAACCAGCGAAAACGCTCCGACTCCGGTCTGA

Upstream 100 bases:

>100_bases
ATAATCAACACAACGGTCATCAAAGCCTTCTAGCCTGTCACGCATATAAAGTCCGCCCATCCGGGCAGACGCACATGCAT
ACCGACTCGCCAGACCTTTG

Downstream 100 bases:

>100_bases
GCACATCGGGAATGGCGGCGTTAGCGACATTCCCTTGAATTGACAGAAACAGCGAGACGGGTGGACTTCGGTCGCCAGTC
TCGCTAAAATGCGACCTTCC

Product: Fatty acid desaturase

Products: NA

Alternate protein names: Delta(12)-desaturase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTAPSDTLSPADADAISQAPNHGTNRPKRPAKNIPLPEPIAFEAPFPNRKTIRSWLIPLGGRSTARALLLFAFDYALFAA
VLAGVVLASPWWAKVGLGVLAGLIIARLFIIGHDACHQSLTDRRGLNKWLGRLTFLPSLTPYSLWEVGHNVVHHGYTNLK
GFDFVWAPYSLEEFRALPRSRRILERIYRNGLGAGLYYLIEIWWFKLFFPSKRQMATRRPIFMWDCALVGAFGAVWIGAL
VWLAVATGQSPLLLVGTGFVLPFLIWNATVGFVLYVHHTHTSVAWYDTKAMWAKAQPFVSTTVHLRFNFGIGAALHHIME
HTAHHVDMSIPLYQLKRAQALLEHALPGRIIIEKFSWRWYFHTAHRCKLYDFKALCWTDFRGRQTSENAPTPV

Sequences:

>Translated_393_residues
MTAPSDTLSPADADAISQAPNHGTNRPKRPAKNIPLPEPIAFEAPFPNRKTIRSWLIPLGGRSTARALLLFAFDYALFAA
VLAGVVLASPWWAKVGLGVLAGLIIARLFIIGHDACHQSLTDRRGLNKWLGRLTFLPSLTPYSLWEVGHNVVHHGYTNLK
GFDFVWAPYSLEEFRALPRSRRILERIYRNGLGAGLYYLIEIWWFKLFFPSKRQMATRRPIFMWDCALVGAFGAVWIGAL
VWLAVATGQSPLLLVGTGFVLPFLIWNATVGFVLYVHHTHTSVAWYDTKAMWAKAQPFVSTTVHLRFNFGIGAALHHIME
HTAHHVDMSIPLYQLKRAQALLEHALPGRIIIEKFSWRWYFHTAHRCKLYDFKALCWTDFRGRQTSENAPTPV
>Mature_392_residues
TAPSDTLSPADADAISQAPNHGTNRPKRPAKNIPLPEPIAFEAPFPNRKTIRSWLIPLGGRSTARALLLFAFDYALFAAV
LAGVVLASPWWAKVGLGVLAGLIIARLFIIGHDACHQSLTDRRGLNKWLGRLTFLPSLTPYSLWEVGHNVVHHGYTNLKG
FDFVWAPYSLEEFRALPRSRRILERIYRNGLGAGLYYLIEIWWFKLFFPSKRQMATRRPIFMWDCALVGAFGAVWIGALV
WLAVATGQSPLLLVGTGFVLPFLIWNATVGFVLYVHHTHTSVAWYDTKAMWAKAQPFVSTTVHLRFNFGIGAALHHIMEH
TAHHVDMSIPLYQLKRAQALLEHALPGRIIIEKFSWRWYFHTAHRCKLYDFKALCWTDFRGRQTSENAPTPV

Specific function: Can introduce a second cis double bond at the Delta(12) position of fatty acid bound to membranes glycerolipids. This enzyme is involved in chilling tolerance because the phase transition temperature of lipids of cellular membranes depends on the degree o

COG id: COG3239

COG function: function code I; Fatty acid desaturase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fatty acid desaturase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005804 [H]

Pfam domain/function: PF00487 FA_desaturase [H]

EC number: NA

Molecular weight: Translated: 44503; Mature: 44372

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPSDTLSPADADAISQAPNHGTNRPKRPAKNIPLPEPIAFEAPFPNRKTIRSWLIPLG
CCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHCCCC
GRSTARALLLFAFDYALFAAVLAGVVLASPWWAKVGLGVLAGLIIARLFIIGHDACHQSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
TDRRGLNKWLGRLTFLPSLTPYSLWEVGHNVVHHGYTNLKGFDFVWAPYSLEEFRALPRS
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCHHH
RRILERIYRNGLGAGLYYLIEIWWFKLFFPSKRQMATRRPIFMWDCALVGAFGAVWIGAL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHH
VWLAVATGQSPLLLVGTGFVLPFLIWNATVGFVLYVHHTHTSVAWYDTKAMWAKAQPFVS
HHHHHHCCCCCEEEEEHHHHHHHHHHHHHEEEEEEEEECCCEEEEEECHHHHHCCCCCEE
TTVHLRFNFGIGAALHHIMEHTAHHVDMSIPLYQLKRAQALLEHALPGRIIIEKFSWRWY
EEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEE
FHTAHRCKLYDFKALCWTDFRGRQTSENAPTPV
EEECCEEEEEECHHHEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAPSDTLSPADADAISQAPNHGTNRPKRPAKNIPLPEPIAFEAPFPNRKTIRSWLIPLG
CCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHCCCC
GRSTARALLLFAFDYALFAAVLAGVVLASPWWAKVGLGVLAGLIIARLFIIGHDACHQSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
TDRRGLNKWLGRLTFLPSLTPYSLWEVGHNVVHHGYTNLKGFDFVWAPYSLEEFRALPRS
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCHHH
RRILERIYRNGLGAGLYYLIEIWWFKLFFPSKRQMATRRPIFMWDCALVGAFGAVWIGAL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHH
VWLAVATGQSPLLLVGTGFVLPFLIWNATVGFVLYVHHTHTSVAWYDTKAMWAKAQPFVS
HHHHHHCCCCCEEEEEHHHHHHHHHHHHHEEEEEEEEECCCEEEEEECHHHHHCCCCCEE
TTVHLRFNFGIGAALHHIMEHTAHHVDMSIPLYQLKRAQALLEHALPGRIIIEKFSWRWY
EEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEE
FHTAHRCKLYDFKALCWTDFRGRQTSENAPTPV
EEECCEEEEEECHHHEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA