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The map label for this gene is phnD [H]

Identifier: 94309719

GI number: 94309719

Start: 849867

End: 850847

Strand: Reverse

Name: phnD [H]

Synonym: Rmet_0774

Alternate gene names: 94309719

Gene position: 850847-849867 (Counterclockwise)

Preceding gene: 94309720

Following gene: 94309718

Centisome position: 21.66

GC content: 62.9

Gene sequence:

>981_bases
ATGCTTCGCAGAACTTTCCTCGCCGTGGTCGCTTCGGGTGTCGTCGCGCTGCCCGCCTTCGCCCAGGACGTCAAGACCCT
GAACATGGGCATCATCTCGACGGAAACGTCGTCGAACCTGAAGTCCGCCTGGCAGCCCGTGATCGACGACCTGAGCCGCG
CACTCGGCGTGCAGGTCAAGCCGTTCTTCGCCTCGGACTACGCCGGCATCATCGAAGGGATGCGCTTCAACAAAGTGCAG
ATCGCCTGGTACGGCAACAAGTCGGCCATGGAAGCCGTGGACCGCGCCAACGGCGAAGTGTTCGCATCGGTGATCGACAA
GGACGGCAACCCGGGCTACTGGTCGCTGCTGATCGCGCGCAAGGATAGCGACCTGAAGTCGGTCGAGGACGTCATCAAGC
GCGGCAAGGACCTGACCTACGGCGCCGGCGATCCGAATTCCACCTCGGGCACCGCCGTGCCGGGCTACTACCTGTGGGCC
GCCAACAAGGTCGATCCGAAGACGCTGTTCAAGGCCGTGCGCATCAGCAACCACGAAACCAACCTGCTGTCGGTGCTGAA
CAAGCAGGTGGACGTGGCCGTGAACAACACCGAGAACTTCGAGCGCTATCGCATCAACACGGGCAAGAACGCCTATGACG
ACGTGCGCGTGCTGTGGAAGTCGCCGCTGATCCCGGCCGATCCGCTGGTCTACCGCAAGGACCTGCCGCCCGAGCTGAAG
AAGAAGATCCAGGACTTCTTCGTCAACTATGGCAAGGGCGCCGATGCCGCACGCGAAAAGCAGGCGCTGGCCACGCTGAC
CTACCAGGGCTTCCGCGTCTCGACCGATGCCCAGCTCGTGCCGATCCGTCAGATCGAACTGGCCAAGGAAAAGGCCAAGA
TCGAATCCGACAGCACGCTGGCCGCTGCCGACAAGGAAAAGAAGCTGGCCGACGTCTCGCGCCGTCTGGCCGAACTCGAC
CGCGCCAGCAGCACGCAGTAA

Upstream 100 bases:

>100_bases
CCACGCCAGCCCATCGCATCGCCGGCCTCGCACTGTCAGCCCCGAATACCCGATTCACCCAACCCGTATTTACCTTCAGC
CTTTCCAAACGGAGTCATCG

Downstream 100 bases:

>100_bases
GCCTCGCCTGAAGCCGATGGCGCCACTCGGGTCGCCATCGGCTTTGCATTTTCCGCTTCAGCTTTACCTCTTCCGACTGC
CCATGACCGCCACCGCCAAG

Product: phosphonate/organophosphate ester ABC transporter subunit periplasmic binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MLRRTFLAVVASGVVALPAFAQDVKTLNMGIISTETSSNLKSAWQPVIDDLSRALGVQVKPFFASDYAGIIEGMRFNKVQ
IAWYGNKSAMEAVDRANGEVFASVIDKDGNPGYWSLLIARKDSDLKSVEDVIKRGKDLTYGAGDPNSTSGTAVPGYYLWA
ANKVDPKTLFKAVRISNHETNLLSVLNKQVDVAVNNTENFERYRINTGKNAYDDVRVLWKSPLIPADPLVYRKDLPPELK
KKIQDFFVNYGKGADAAREKQALATLTYQGFRVSTDAQLVPIRQIELAKEKAKIESDSTLAAADKEKKLADVSRRLAELD
RASSTQ

Sequences:

>Translated_326_residues
MLRRTFLAVVASGVVALPAFAQDVKTLNMGIISTETSSNLKSAWQPVIDDLSRALGVQVKPFFASDYAGIIEGMRFNKVQ
IAWYGNKSAMEAVDRANGEVFASVIDKDGNPGYWSLLIARKDSDLKSVEDVIKRGKDLTYGAGDPNSTSGTAVPGYYLWA
ANKVDPKTLFKAVRISNHETNLLSVLNKQVDVAVNNTENFERYRINTGKNAYDDVRVLWKSPLIPADPLVYRKDLPPELK
KKIQDFFVNYGKGADAAREKQALATLTYQGFRVSTDAQLVPIRQIELAKEKAKIESDSTLAAADKEKKLADVSRRLAELD
RASSTQ
>Mature_326_residues
MLRRTFLAVVASGVVALPAFAQDVKTLNMGIISTETSSNLKSAWQPVIDDLSRALGVQVKPFFASDYAGIIEGMRFNKVQ
IAWYGNKSAMEAVDRANGEVFASVIDKDGNPGYWSLLIARKDSDLKSVEDVIKRGKDLTYGAGDPNSTSGTAVPGYYLWA
ANKVDPKTLFKAVRISNHETNLLSVLNKQVDVAVNNTENFERYRINTGKNAYDDVRVLWKSPLIPADPLVYRKDLPPELK
KKIQDFFVNYGKGADAAREKQALATLTYQGFRVSTDAQLVPIRQIELAKEKAKIESDSTLAAADKEKKLADVSRRLAELD
RASSTQ

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=317, Percent_Identity=51.4195583596215, Blast_Score=322, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017797
- InterPro:   IPR005770 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 35996; Mature: 35996

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRTFLAVVASGVVALPAFAQDVKTLNMGIISTETSSNLKSAWQPVIDDLSRALGVQVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEC
PFFASDYAGIIEGMRFNKVQIAWYGNKSAMEAVDRANGEVFASVIDKDGNPGYWSLLIAR
CHHHCCHHHHHCCCEECEEEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEE
KDSDLKSVEDVIKRGKDLTYGAGDPNSTSGTAVPGYYLWAANKVDPKTLFKAVRISNHET
CCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCH
NLLSVLNKQVDVAVNNTENFERYRINTGKNAYDDVRVLWKSPLIPADPLVYRKDLPPELK
HHHHHHHHHHEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEECCCCCHHHH
KKIQDFFVNYGKGADAAREKQALATLTYQGFRVSTDAQLVPIRQIELAKEKAKIESDSTL
HHHHHHHHHCCCCCCHHHHHHHHHHEEECCEEECCCCEEEEHHHHHHHHHHHHCCCCCCE
AAADKEKKLADVSRRLAELDRASSTQ
ECCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLRRTFLAVVASGVVALPAFAQDVKTLNMGIISTETSSNLKSAWQPVIDDLSRALGVQVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEC
PFFASDYAGIIEGMRFNKVQIAWYGNKSAMEAVDRANGEVFASVIDKDGNPGYWSLLIAR
CHHHCCHHHHHCCCEECEEEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEE
KDSDLKSVEDVIKRGKDLTYGAGDPNSTSGTAVPGYYLWAANKVDPKTLFKAVRISNHET
CCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCH
NLLSVLNKQVDVAVNNTENFERYRINTGKNAYDDVRVLWKSPLIPADPLVYRKDLPPELK
HHHHHHHHHHEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEECCCCCHHHH
KKIQDFFVNYGKGADAAREKQALATLTYQGFRVSTDAQLVPIRQIELAKEKAKIESDSTL
HHHHHHHHHCCCCCCHHHHHHHHHHEEECCEEECCCCEEEEHHHHHHHHHHHHCCCCCCE
AAADKEKKLADVSRRLAELDRASSTQ
ECCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]