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The map label for this gene is phnF [C]

Identifier: 94309712

GI number: 94309712

Start: 844433

End: 845188

Strand: Direct

Name: phnF [C]

Synonym: Rmet_0767

Alternate gene names: 94309712

Gene position: 844433-845188 (Clockwise)

Preceding gene: 94309703

Following gene: 94309713

Centisome position: 21.5

GC content: 62.7

Gene sequence:

>756_bases
ATGACAACAGGGCAAGGCAAGATGTCTGATCGGGAAGTCGAACGGGGTTCCGGCGTCGCCGTATGGCGGCAGATTGGCGA
GGCGCTTGCGGAGGACATCCGCAACAAACTCTATGGGCCGGGCGAGCAGTTGCCGCCGGAACCGGAACTGGCCTCGCGCT
TCGCTGTGAACCGCCATACGATCCGGCGCGCGATGGGTGAGCTCGAACTGAGCGGCCTGGTGCGGATCGAGCAGGGCCGA
GGCACCTTCGTGCAGGAGCACGCCATTGATTACGCCATCGGGCGTCGCACGCGCTTCTCGCAGAACCTGGCCGCGCAGGG
GATGCGCGGGCACGCGGAACTCGTCAATAGCCAGATCCTGCGCGCGCCGGAAATCGCCAAACATCTTGGTCTGTCCCGTA
CGGCGGAGATTCTGCATGTGCAGATGCTCGGCAAGGCCGAAGGGCGCACGGTCGACGTGGCCGAGCATTACTTCGATCCT
AAGCGTTTTCCCGGTTTTGAAGACAAGGTGAACGAGACACAATCGATCTCGCGCACGCTGGCGAAGTACGGCATTGCCGA
TTACACCCGCAAGTGGTCGCGCATCACGGCGGCCATGCCGAGCGCCCCGGTGGCGCGATTGCTGAATCAGCCGAAGACGC
GGCCTATCCTGCAAGTGGAGGCACTGAACATCGATATTGACGGCGCCCCCGTCCAATACAGCATGACGCGCTTTGCCGGC
GACTGGGTGCAACTCACGGTCTCCGATCAGGATTGA

Upstream 100 bases:

>100_bases
CAGGCACATGAACGTTTCGTGACGGGTCGGGCCTTTCCTCGGACACGCAACATCGGCGCCAAGGCATGAGGCACAATCGC
ACGCTATTGGTGAATGCTTG

Downstream 100 bases:

>100_bases
AATGACGCATCACCCCGCGGTTGCACGCGGGGTGATGCGTACGTATTGGTCTAGACATCCGTCTGTCATCTACCAAAGCT
AACGTATTGCCCATGCATAC

Product: putative DNA-binding transcriptional regulator of phosphonate uptake and biodegradation

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MTTGQGKMSDREVERGSGVAVWRQIGEALAEDIRNKLYGPGEQLPPEPELASRFAVNRHTIRRAMGELELSGLVRIEQGR
GTFVQEHAIDYAIGRRTRFSQNLAAQGMRGHAELVNSQILRAPEIAKHLGLSRTAEILHVQMLGKAEGRTVDVAEHYFDP
KRFPGFEDKVNETQSISRTLAKYGIADYTRKWSRITAAMPSAPVARLLNQPKTRPILQVEALNIDIDGAPVQYSMTRFAG
DWVQLTVSDQD

Sequences:

>Translated_251_residues
MTTGQGKMSDREVERGSGVAVWRQIGEALAEDIRNKLYGPGEQLPPEPELASRFAVNRHTIRRAMGELELSGLVRIEQGR
GTFVQEHAIDYAIGRRTRFSQNLAAQGMRGHAELVNSQILRAPEIAKHLGLSRTAEILHVQMLGKAEGRTVDVAEHYFDP
KRFPGFEDKVNETQSISRTLAKYGIADYTRKWSRITAAMPSAPVARLLNQPKTRPILQVEALNIDIDGAPVQYSMTRFAG
DWVQLTVSDQD
>Mature_250_residues
TTGQGKMSDREVERGSGVAVWRQIGEALAEDIRNKLYGPGEQLPPEPELASRFAVNRHTIRRAMGELELSGLVRIEQGRG
TFVQEHAIDYAIGRRTRFSQNLAAQGMRGHAELVNSQILRAPEIAKHLGLSRTAEILHVQMLGKAEGRTVDVAEHYFDPK
RFPGFEDKVNETQSISRTLAKYGIADYTRKWSRITAAMPSAPVARLLNQPKTRPILQVEALNIDIDGAPVQYSMTRFAGD
WVQLTVSDQD

Specific function: Belongs To An Operon Involved In Alkylphosphonate Uptake And C-P Lyase. Exact Function Not Known. By Similarity Could Be A Transcriptional Regulator. [C]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790540, Length=231, Percent_Identity=32.4675324675325, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1786950, Length=237, Percent_Identity=26.1603375527426, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082252, Length=237, Percent_Identity=21.5189873417722, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788418, Length=225, Percent_Identity=22.6666666666667, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012702
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 28019; Mature: 27888

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGQGKMSDREVERGSGVAVWRQIGEALAEDIRNKLYGPGEQLPPEPELASRFAVNRHT
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
IRRAMGELELSGLVRIEQGRGTFVQEHAIDYAIGRRTRFSQNLAAQGMRGHAELVNSQIL
HHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHH
RAPEIAKHLGLSRTAEILHVQMLGKAEGRTVDVAEHYFDPKRFPGFEDKVNETQSISRTL
CHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHH
AKYGIADYTRKWSRITAAMPSAPVARLLNQPKTRPILQVEALNIDIDGAPVQYSMTRFAG
HHHCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCHHHHHHCC
DWVQLTVSDQD
CEEEEEECCCC
>Mature Secondary Structure 
TTGQGKMSDREVERGSGVAVWRQIGEALAEDIRNKLYGPGEQLPPEPELASRFAVNRHT
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
IRRAMGELELSGLVRIEQGRGTFVQEHAIDYAIGRRTRFSQNLAAQGMRGHAELVNSQIL
HHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHH
RAPEIAKHLGLSRTAEILHVQMLGKAEGRTVDVAEHYFDPKRFPGFEDKVNETQSISRTL
CHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHH
AKYGIADYTRKWSRITAAMPSAPVARLLNQPKTRPILQVEALNIDIDGAPVQYSMTRFAG
HHHCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCHHHHHHCC
DWVQLTVSDQD
CEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431; 9889346 [H]