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The map label for this gene is dmlR [H]

Identifier: 94309688

GI number: 94309688

Start: 821700

End: 822632

Strand: Reverse

Name: dmlR [H]

Synonym: Rmet_0743

Alternate gene names: 94309688

Gene position: 822632-821700 (Counterclockwise)

Preceding gene: 94309689

Following gene: 94309687

Centisome position: 20.94

GC content: 66.88

Gene sequence:

>933_bases
ATGCCTTTCGACCTGACCGATATCGCGGTCTTCGTGCGCGCGGCGGCGCTCGGCAATCTGTCGGCTGCCGCACGCGATCT
GGGACTGTCGACATCGACCGCCAGCGCCCGGCTCCAGCGACTGGAGGATCGGCTAGGCACCCGCCTGCTACATCGCACAA
CGCGCCGCCTTTCGCTCACCGGAGATGGTGAGCGGTTCCTGACCCACGCCGAGCCATTGCTGGCCGCATCGGAAGTGGCC
GCGCAATCCGTGGGCCGAGGCGCGGAGGTGCCGCACGGTCCGTTGCGTGTCACGGCGCCTGCCTCGTTCGGACGCCAGCA
TGTCTCGCCGGCGATCCCCGCCTTCCTGGCCACCTACCCCGGCATCACACTGGATCTGCGCCTGAGCGACCAGATCGTCG
GCCTCGTCGATAGTGGCATTGATGTCGCGCTCCGCATGGGCGCGCTCCCCGATTCCTCGCTGGTCGCCAGGCCACTCGCG
CCCAGTCATCGTGTGATCTGCGCATCGCCGGAATACCTCGCGCGGCACGGCTCCCCACGGCACCCGGAAGACCTGCGCAA
TCACAACTGCCTGGTCCTTGGCGATCAATCGACCTGGCGTTTTGATACCCCGGTCGGCGAAAGCGCGGTGAGCGTGCGGG
GCACGCTACGCGCCGATAACGGCGAGGTGATTCGCGACGCGATCGTCGCGGGCGTGGGCATCGCGCTGAAGTCGACCTGG
GATGTGGCGCCATACCTGCGCCGCGGCGAGTTGGTCACCGTGCTCGATGAATACCCGGTATTGCCGGTCGTCTCGATCTG
GGCGGTCTATCCGAGCCGGCATCTGGTTCCCGCCAAGACACACGCCTTTGTCGACTTCTTTGCCAACTGGTTCGGCTCCC
CGCCCTACTGGGATCGCGACGACGCGCCTCCCTTGGCCACACGTACCGTCTGA

Upstream 100 bases:

>100_bases
TTGCCATCAGCTTCCAGACGTTCTTCGAATCGCAAGTCGAGGCAGCCAAGGCGCTCTCGCAGGAATAGCCGAAGGCCTGG
CCCCACTTTACGCGCCGACG

Downstream 100 bases:

>100_bases
CCAGATCTGAAGCCCACCGGAGGTTCAATCCCCAGGGCGCGCATTCCATCAGGGCGCTGATCAGGGCACTGGTATCTCGC
CAGCAATACTGTATATTTAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MPFDLTDIAVFVRAAALGNLSAAARDLGLSTSTASARLQRLEDRLGTRLLHRTTRRLSLTGDGERFLTHAEPLLAASEVA
AQSVGRGAEVPHGPLRVTAPASFGRQHVSPAIPAFLATYPGITLDLRLSDQIVGLVDSGIDVALRMGALPDSSLVARPLA
PSHRVICASPEYLARHGSPRHPEDLRNHNCLVLGDQSTWRFDTPVGESAVSVRGTLRADNGEVIRDAIVAGVGIALKSTW
DVAPYLRRGELVTVLDEYPVLPVVSIWAVYPSRHLVPAKTHAFVDFFANWFGSPPYWDRDDAPPLATRTV

Sequences:

>Translated_310_residues
MPFDLTDIAVFVRAAALGNLSAAARDLGLSTSTASARLQRLEDRLGTRLLHRTTRRLSLTGDGERFLTHAEPLLAASEVA
AQSVGRGAEVPHGPLRVTAPASFGRQHVSPAIPAFLATYPGITLDLRLSDQIVGLVDSGIDVALRMGALPDSSLVARPLA
PSHRVICASPEYLARHGSPRHPEDLRNHNCLVLGDQSTWRFDTPVGESAVSVRGTLRADNGEVIRDAIVAGVGIALKSTW
DVAPYLRRGELVTVLDEYPVLPVVSIWAVYPSRHLVPAKTHAFVDFFANWFGSPPYWDRDDAPPLATRTV
>Mature_309_residues
PFDLTDIAVFVRAAALGNLSAAARDLGLSTSTASARLQRLEDRLGTRLLHRTTRRLSLTGDGERFLTHAEPLLAASEVAA
QSVGRGAEVPHGPLRVTAPASFGRQHVSPAIPAFLATYPGITLDLRLSDQIVGLVDSGIDVALRMGALPDSSLVARPLAP
SHRVICASPEYLARHGSPRHPEDLRNHNCLVLGDQSTWRFDTPVGESAVSVRGTLRADNGEVIRDAIVAGVGIALKSTWD
VAPYLRRGELVTVLDEYPVLPVVSIWAVYPSRHLVPAKTHAFVDFFANWFGSPPYWDRDDAPPLATRTV

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=293, Percent_Identity=37.2013651877133, Blast_Score=190, Evalue=9e-50,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=36.4864864864865, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI1789440, Length=289, Percent_Identity=33.9100346020761, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=29.7945205479452, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=32.7586206896552, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789639, Length=291, Percent_Identity=28.1786941580756, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787589, Length=289, Percent_Identity=28.3737024221453, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788706, Length=261, Percent_Identity=26.8199233716475, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790011, Length=287, Percent_Identity=24.7386759581882, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789173, Length=263, Percent_Identity=26.615969581749, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=24.9134948096886, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=245, Percent_Identity=27.7551020408163, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082132, Length=281, Percent_Identity=22.0640569395018, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790399, Length=258, Percent_Identity=25.5813953488372, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787879, Length=158, Percent_Identity=32.9113924050633, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33478; Mature: 33347

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFDLTDIAVFVRAAALGNLSAAARDLGLSTSTASARLQRLEDRLGTRLLHRTTRRLSLT
CCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEC
GDGERFLTHAEPLLAASEVAAQSVGRGAEVPHGPLRVTAPASFGRQHVSPAIPAFLATYP
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHCHHHCCHHHHHHHHHCC
GITLDLRLSDQIVGLVDSGIDVALRMGALPDSSLVARPLAPSHRVICASPEYLARHGSPR
CCEEEEEECCCCHHHHCCCCEEEEEECCCCCCHHHCCCCCCCCCEEECCHHHHHHCCCCC
HPEDLRNHNCLVLGDQSTWRFDTPVGESAVSVRGTLRADNGEVIRDAIVAGVGIALKSTW
CHHHHCCCCEEEECCCCCEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCEEECCC
DVAPYLRRGELVTVLDEYPVLPVVSIWAVYPSRHLVPAKTHAFVDFFANWFGSPPYWDRD
CCCHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
DAPPLATRTV
CCCCCCCCCC
>Mature Secondary Structure 
PFDLTDIAVFVRAAALGNLSAAARDLGLSTSTASARLQRLEDRLGTRLLHRTTRRLSLT
CCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEC
GDGERFLTHAEPLLAASEVAAQSVGRGAEVPHGPLRVTAPASFGRQHVSPAIPAFLATYP
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHCHHHCCHHHHHHHHHCC
GITLDLRLSDQIVGLVDSGIDVALRMGALPDSSLVARPLAPSHRVICASPEYLARHGSPR
CCEEEEEECCCCHHHHCCCCEEEEEECCCCCCHHHCCCCCCCCCEEECCHHHHHHCCCCC
HPEDLRNHNCLVLGDQSTWRFDTPVGESAVSVRGTLRADNGEVIRDAIVAGVGIALKSTW
CHHHHCCCCEEEECCCCCEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCEEECCC
DVAPYLRRGELVTVLDEYPVLPVVSIWAVYPSRHLVPAKTHAFVDFFANWFGSPPYWDRD
CCCHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
DAPPLATRTV
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]