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The map label for this gene is rfaD

Identifier: 94309673

GI number: 94309673

Start: 805508

End: 806503

Strand: Direct

Name: rfaD

Synonym: Rmet_0728

Alternate gene names: 94309673

Gene position: 805508-806503 (Clockwise)

Preceding gene: 94309672

Following gene: 94309674

Centisome position: 20.51

GC content: 61.35

Gene sequence:

>996_bases
ATGACCATCATCGTGACCGGCGCTGCCGGCTTTATCGGCAGCAACATCGTCAAGGGCCTGAACGAGCGCGGCGAGACGAA
CATCATCGCCGTGGACAACCTGACCCGCGCCGAGAAGTTCAACAATCTCGTCGACTGCGAGATTGCCGACTACCTGGACA
AGACCGACTTCGTCGCCCGCTTCGCGCGCGGCGATTTCGGCAATGTCCGCGCGGTATTCCATGAGGGCGCCTGCTCCGAC
ACGATGGAGACCGACGGTCGCTACATGATGGAGAACAACTACCGCTACACGCTTGCCTTGCTGGAGGCGTGCCTGGAGCA
GGGTGCCCAGTTCCTGTACGCGTCGTCGGCGGCCACTTATGGCGCTTCGACGATGTTTCGCGAGGACCGTGACTACGAAA
AGCCGCTGAACGTCTACGGCTACTCCAAGTTCCTGTTTGACCAGGTGGTGCGCCGTCGCCTGCCTTCCGCCCATTCGCAG
ATCGTGGGCTTCCGCTACTTCAACGTCTACGGGCCGCGCGAATTCCACAAGGGCCGCATGGCGTCGGTGGCGTTCCATCA
CTTCAACCAGTTCCGCGCCGAAGGTACGGTCAAGCTGTTTGGCGAGTACAACGGCTATGCGCCGGGCACGCAGAGCCGGG
ACTTCGTGTCCGTGGAGGACGTGGTCAAGGTTAATCTGTACTTCCTGGACCATCCCGAGAAGTCCGGCATCTTCAACCTC
GGTACTGGGCGGTCGCAGCCGTTCAACGACATCGCCGTCTCGGTGGTCAATGCGCTGCGCGAGTCGGAAGGCAAGCCGGC
GCTCTCGCTCGATGAAATGGTGCAGGAAGGGCTGGTCGAGTACGTCAAGTTCCCTGATGCCCTGCGTGGCAAGTATCAAT
GCTTCACGCAGTCGGATGTATCGCGCCTGCGTGGGGCTGGCTACAGCGACCAGTTCCTGACCGTGCAGGAAGGCGTCAGC
CGGTACTGCAAGTGGCTGCTGGAGCGAAGCGCCTAA

Upstream 100 bases:

>100_bases
GGGCCGGCGCCATTGTTGTTGGCAAGCTGGGTACCGCCGTTGTTACTTATTCCGAATTGTTCGGCGCTGCCTGAGCGGCG
CCACCGAGCCTGAATCCGCC

Downstream 100 bases:

>100_bases
GGTTCGTTTGCAGGGTGCGTTCATCGTCCCCTGTTGCGTGAAAGCCGCGGTCTGCAACGCGGTTATTTCGATTCGACACA
CAATGGCGCGAAGGTTGTGA

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTIIVTGAAGFIGSNIVKGLNERGETNIIAVDNLTRAEKFNNLVDCEIADYLDKTDFVARFARGDFGNVRAVFHEGACSD
TMETDGRYMMENNYRYTLALLEACLEQGAQFLYASSAATYGASTMFREDRDYEKPLNVYGYSKFLFDQVVRRRLPSAHSQ
IVGFRYFNVYGPREFHKGRMASVAFHHFNQFRAEGTVKLFGEYNGYAPGTQSRDFVSVEDVVKVNLYFLDHPEKSGIFNL
GTGRSQPFNDIAVSVVNALRESEGKPALSLDEMVQEGLVEYVKFPDALRGKYQCFTQSDVSRLRGAGYSDQFLTVQEGVS
RYCKWLLERSA

Sequences:

>Translated_331_residues
MTIIVTGAAGFIGSNIVKGLNERGETNIIAVDNLTRAEKFNNLVDCEIADYLDKTDFVARFARGDFGNVRAVFHEGACSD
TMETDGRYMMENNYRYTLALLEACLEQGAQFLYASSAATYGASTMFREDRDYEKPLNVYGYSKFLFDQVVRRRLPSAHSQ
IVGFRYFNVYGPREFHKGRMASVAFHHFNQFRAEGTVKLFGEYNGYAPGTQSRDFVSVEDVVKVNLYFLDHPEKSGIFNL
GTGRSQPFNDIAVSVVNALRESEGKPALSLDEMVQEGLVEYVKFPDALRGKYQCFTQSDVSRLRGAGYSDQFLTVQEGVS
RYCKWLLERSA
>Mature_330_residues
TIIVTGAAGFIGSNIVKGLNERGETNIIAVDNLTRAEKFNNLVDCEIADYLDKTDFVARFARGDFGNVRAVFHEGACSDT
METDGRYMMENNYRYTLALLEACLEQGAQFLYASSAATYGASTMFREDRDYEKPLNVYGYSKFLFDQVVRRRLPSAHSQI
VGFRYFNVYGPREFHKGRMASVAFHHFNQFRAEGTVKLFGEYNGYAPGTQSRDFVSVEDVVKVNLYFLDHPEKSGIFNLG
TGRSQPFNDIAVSVVNALRESEGKPALSLDEMVQEGLVEYVKFPDALRGKYQCFTQSDVSRLRGAGYSDQFLTVQEGVSR
YCKWLLERSA

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily

Homologues:

Organism=Escherichia coli, GI1790049, Length=328, Percent_Identity=56.4024390243902, Blast_Score=347, Evalue=5e-97,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): HLDD_RALME (Q1LQG2)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582883.1
- ProteinModelPortal:   Q1LQG2
- SMR:   Q1LQG2
- STRING:   Q1LQG2
- GeneID:   4037519
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0728
- eggNOG:   COG0451
- HOGENOM:   HBG755066
- OMA:   GFIGSAM
- PhylomeDB:   Q1LQG2
- ProtClustDB:   CLSK896740
- BioCyc:   RMET266264:RMET_0728-MONOMER
- HAMAP:   MF_01601
- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10366:SF29
- TIGRFAMs:   TIGR02197

Pfam domain/function: PF01370 Epimerase

EC number: =5.1.3.20

Molecular weight: Translated: 37354; Mature: 37223

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: ACT_SITE 139-139 ACT_SITE 177-177 BINDING 39-39 BINDING 54-54 BINDING 92-92 BINDING 143-143 BINDING 168-168 BINDING 169-169 BINDING 177-177 BINDING 179-179 BINDING 186-186 BINDING 213-213 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIVTGAAGFIGSNIVKGLNERGETNIIAVDNLTRAEKFNNLVDCEIADYLDKTDFVAR
CEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHH
FARGDFGNVRAVFHEGACSDTMETDGRYMMENNYRYTLALLEACLEQGAQFLYASSAATY
HHCCCCCCEEEEEECCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEECCCHHH
GASTMFREDRDYEKPLNVYGYSKFLFDQVVRRRLPSAHSQIVGFRYFNVYGPREFHKGRM
CHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEEEEEECCCHHHHCCHH
ASVAFHHFNQFRAEGTVKLFGEYNGYAPGTQSRDFVSVEDVVKVNLYFLDHPEKSGIFNL
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHEEEEEEEEEECCCCCCEEEC
GTGRSQPFNDIAVSVVNALRESEGKPALSLDEMVQEGLVEYVKFPDALRGKYQCFTQSDV
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCEEECHHHH
SRLRGAGYSDQFLTVQEGVSRYCKWLLERSA
HHHHCCCCCCCEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIIVTGAAGFIGSNIVKGLNERGETNIIAVDNLTRAEKFNNLVDCEIADYLDKTDFVAR
EEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHH
FARGDFGNVRAVFHEGACSDTMETDGRYMMENNYRYTLALLEACLEQGAQFLYASSAATY
HHCCCCCCEEEEEECCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEECCCHHH
GASTMFREDRDYEKPLNVYGYSKFLFDQVVRRRLPSAHSQIVGFRYFNVYGPREFHKGRM
CHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEEEEEECCCHHHHCCHH
ASVAFHHFNQFRAEGTVKLFGEYNGYAPGTQSRDFVSVEDVVKVNLYFLDHPEKSGIFNL
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHEEEEEEEEEECCCCCCEEEC
GTGRSQPFNDIAVSVVNALRESEGKPALSLDEMVQEGLVEYVKFPDALRGKYQCFTQSDV
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCEEECHHHH
SRLRGAGYSDQFLTVQEGVSRYCKWLLERSA
HHHHCCCCCCCEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA