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The map label for this gene is gph [H]

Identifier: 94309655

GI number: 94309655

Start: 785930

End: 786586

Strand: Reverse

Name: gph [H]

Synonym: Rmet_0710

Alternate gene names: 94309655

Gene position: 786586-785930 (Counterclockwise)

Preceding gene: 94309656

Following gene: 94309653

Centisome position: 20.02

GC content: 65.6

Gene sequence:

>657_bases
ATGATGACGTTCGACGCAGTTTTCTTCGATCTTGACGGCACACTTGCCGATACCGCTCCTGACCTCGCCGCAGCCGCGAA
CCGGCTGGTCATCAAACATGGCATGTTGCCGGTCGCGTACGAACGGCTTCGTCCGGTAGCTTCGCACGGCGCGCGTGGCC
TGCTCGGCGTGGCGTTCGGCAAGCGCCCGGAGGATCCCGAATTCCCGGCCTTGCGCGACGCGTTCCTTGACTACTACGAG
GCCGATATCGCCGTCCATACGCGCCTGTTCGAGGGCATGGACCAGGTGCTGGCACAACTGGAGTCCACGGGCATTCGCTG
GGGCATCGTGACCAACAAGATTGCCCGCTTCACCGTGCCACTGGTCAATGCGATCGGCCTGACGCCACGCGCCTCGGCCG
TGGTCAGCGGTGACACCACCCCTTACGCCAAGCCACACCCCGCACCGCTGCTGCGAGCGGCAGAATTGAGCGGCGTGGCG
CCCGGCCGGTGCATATACGTCGGCGACGACCTGCGCGACATTCAGGCTGGCCAGGCCGCCGGCATGACCACGGTCGCCGC
GGCATACGGCTACTGCGGCGAAGCCGAACCTCCGGAAACCTGGGGCGCCGACTACCTGATCCGCCATCCCGCGGAGCTGC
TACCACTATTCTCCTGA

Upstream 100 bases:

>100_bases
CGAGATGCGCGGCATGACCTACAACCCGTTGACGCAGATCTACTCGCTTGGCCGCGACACCGACGTCAATTACATGATGG
CTTTCCGCAAGCCTTGAACC

Downstream 100 bases:

>100_bases
CCAAGGAACCGAACCACACAGGGTGCGTCGAAAGCGAGTACAATCCGTTTTCTTTCGCGTTTTGACGCACATTGGGGCCG
ACCTGGTTTCGACGTGGGTT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MMTFDAVFFDLDGTLADTAPDLAAAANRLVIKHGMLPVAYERLRPVASHGARGLLGVAFGKRPEDPEFPALRDAFLDYYE
ADIAVHTRLFEGMDQVLAQLESTGIRWGIVTNKIARFTVPLVNAIGLTPRASAVVSGDTTPYAKPHPAPLLRAAELSGVA
PGRCIYVGDDLRDIQAGQAAGMTTVAAAYGYCGEAEPPETWGADYLIRHPAELLPLFS

Sequences:

>Translated_218_residues
MMTFDAVFFDLDGTLADTAPDLAAAANRLVIKHGMLPVAYERLRPVASHGARGLLGVAFGKRPEDPEFPALRDAFLDYYE
ADIAVHTRLFEGMDQVLAQLESTGIRWGIVTNKIARFTVPLVNAIGLTPRASAVVSGDTTPYAKPHPAPLLRAAELSGVA
PGRCIYVGDDLRDIQAGQAAGMTTVAAAYGYCGEAEPPETWGADYLIRHPAELLPLFS
>Mature_218_residues
MMTFDAVFFDLDGTLADTAPDLAAAANRLVIKHGMLPVAYERLRPVASHGARGLLGVAFGKRPEDPEFPALRDAFLDYYE
ADIAVHTRLFEGMDQVLAQLESTGIRWGIVTNKIARFTVPLVNAIGLTPRASAVVSGDTTPYAKPHPAPLLRAAELSGVA
PGRCIYVGDDLRDIQAGQAAGMTTVAAAYGYCGEAEPPETWGADYLIRHPAELLPLFS

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=211, Percent_Identity=39.8104265402844, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23366; Mature: 23366

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTFDAVFFDLDGTLADTAPDLAAAANRLVIKHGMLPVAYERLRPVASHGARGLLGVAFG
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCC
KRPEDPEFPALRDAFLDYYEADIAVHTRLFEGMDQVLAQLESTGIRWGIVTNKIARFTVP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
LVNAIGLTPRASAVVSGDTTPYAKPHPAPLLRAAELSGVAPGRCIYVGDDLRDIQAGQAA
HHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCC
GMTTVAAAYGYCGEAEPPETWGADYLIRHPAELLPLFS
CHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHCCCCC
>Mature Secondary Structure
MMTFDAVFFDLDGTLADTAPDLAAAANRLVIKHGMLPVAYERLRPVASHGARGLLGVAFG
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCC
KRPEDPEFPALRDAFLDYYEADIAVHTRLFEGMDQVLAQLESTGIRWGIVTNKIARFTVP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
LVNAIGLTPRASAVVSGDTTPYAKPHPAPLLRAAELSGVAPGRCIYVGDDLRDIQAGQAA
HHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCC
GMTTVAAAYGYCGEAEPPETWGADYLIRHPAELLPLFS
CHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]