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The map label for this gene is pilJ [H]

Identifier: 94309617

GI number: 94309617

Start: 737922

End: 740123

Strand: Direct

Name: pilJ [H]

Synonym: Rmet_0672

Alternate gene names: 94309617

Gene position: 737922-740123 (Clockwise)

Preceding gene: 94309616

Following gene: 94309618

Centisome position: 18.79

GC content: 66.49

Gene sequence:

>2202_bases
ATGGGTATCAAGAAGATCGGCCTGGGTCGCCGCACCGCAGCCGCGGGCGCTGCCGCAGAAGTGGCGGCGCCGGGTGGCGC
CGCGTTTGCGGCGGCCGGCGCGGCCAGACAGCAGGGCGGCCTTGGCCAGACCCCTGTCGAGATGATGTCGGGCTGGCTGA
CGCGGATGCCGTTCTCGGCGCAGCAGCGCGCGCTGACCGTGGGCGTGGTGGTGTCGCTGTCGGCGCTGCTCGCCTCCGTG
TACCTTGATAACCGGCAGGCCAGCAACGGCGCGGCGCAGATCGAGGTGACCGGCGACATGCTGATGCACTCGCAGCGTCT
CGGTAAGGCGGTGCCCGTGGCGCTGCTCGGCAACGCCCAGGCGTTCAAGCAGCTGCGCGAGTCGAAAGAGCAGCTGACCA
CCGACATGCTGGCGCTGCAGAACGGCAGCGACGAGAAGCATGTGCGGGCCACGAGCGGGCCGGGCGAGCCGCTGCTGGAA
AAGGCGATGGGCTCGTGGAAGCGTTCCGAGAAGAGCGCCGCCGACGTGCTCGCCCAGCAGGCCATTCTGACGACGATCGG
CCAGACGCTGCAGATCTTCAACGCGTCCAACCCCGAACTGCTCGAAGCCGCTGAACAGGTGGCGGCCATCAAGCTGCAGT
CGGGCGCCAACACCCGTGAGGTGGCGGCTTCCGCTCAGTTGGTGATGCTGACGCAGCGTCTGGGCAAGAACCTCAACGAG
TTCCTCTCGGGCGAAGGCGTCAACCCTGAAACCGCGTTCCTGCTTGGCAAGGACACCAACACCTTCCGCGAAACACTCGA
CGGCCTGATCAACGGCTCCGAAGCGCTGCGCCTGACCGCCGCGCACGATGCCGAGACGCGCAGCTACCTGCAGCAACTTT
CGCAGCGCTTCGACGCGGTGCAGAAGACCACGCAGACGATTCTGCAGAACCTGCCTGGTCTGATCGCCGCCAAGCGCGCC
CAGCAGCAGATCTTCAATGACAACGAAGCCCTGCGCGGCGAGTTGTCGGCGTTGCAGTCGGCCTATGCGCAAGATGCGCG
CTTCCGCCCCTGGACACTGGGCGCGATGGTTATCTCCGCGCTAGCCACGCTGCTGTGCCTGGCCGGTCTCGCGGCCCTCT
ACCTGCGCGACTCGCGCGTCCGGGCGCTCGAAGCCGAGGCGCGCGAGCACGAAGCCGAAGCGCGCCGACTCGATGAAAAG
CGCAATAACGACGCCACCCAGAAGGCCATTCTGCAGCTGATGAACGAGCTGCAGGACATTGCTGACGGTGACTTGACGCG
TCAGGCCACTGTGACCGAGGACATCACCGGCGCCATTGCCGACTCGGTGAACTACACGGTGGAAGAACTGAAGGAACTGG
TGGGCCGCGTGCAGCAGACCGCCGGGGACGTGCAGCACGCCTCGACGCAGGTGCAGGAAACCTCGGTGCAACTGGCCACC
ACGACCGAGCAGCAGTCGCGCCAGATTCGTCAGACCGGCGAATCCGTGGTGGAGATGGCGGACCGCATCACGCAGGTATC
ACGCGGTGCGGCCGAATCGGCCAACGTGGCACGCGCATCGCTGGCTGCGGCCGAGCAGGGCCAGCACGCCGTGCAGAACG
CGATCGCGGGCATGAACGGCATTCGCGAGCAGATCCAGGAAACGTCCAAGCGGATCAAGCGTCTCGGCGAATCGTCGCAG
GAGATCGGTGAAATCGTCGAGCTGATCTCGGACATTACCGAACAGACCAACGTGCTGGCACTGAACGCCGCCATCCAGGC
GGCTTCCGCAGGCGAAGCCGGCCGCGGCTTCTCGGTGGTGGCGGAAGAAGTGCAGCGTCTGGCCGAACGTTCGGGCGAGG
CAACCAAGCAGATCGGCGCGCTGATTCGCACGATTCAGACCGACACTCAGGACGCCGTGCACGCGATGGAGCAGAGCACG
CAGGGCGTGGTCGAGGGCGCGCGCCTGTCCGATAACGCGGGTGCCGCACTGGTCGAGATCGGTCGCGTGTCGCGCCAGCT
CGCCGAGCTGATCGAACAGATCTCGCAGACCACATCGCACGAAGCAGGCCTGGCCACCAACGTGGCCCGCCACATCGACG
GCATCCTGCAGGTGACCGCGCAGACCACCGCCGGCACGCGCCAGACCGCCGCGTCGGTGCGCCAGCTGACCGCACTGACC
GAAGAGCTGCGTAACTCGGTATCGCGGTTCAAGATCGCCTGA

Upstream 100 bases:

>100_bases
CGCGGGGCCGCAGACCGTACAAGTGGTATGGCAAGGCCCCGCAACGACGCCGGAATCATTCTGAAGCGGTTTCATAATAA
CGAACGAACAGAGGGTTGCT

Downstream 100 bases:

>100_bases
CGGCCGCCCGCAAGGGCGCAACGGAACTGGTCATGTCTTTCAATACCATCCTGTCGAGCGAAGTGGCCGAGACGGCCGCG
GCGCAACTGTCATCCACGCC

Product: type IV pilus transmembrane protein PilJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MGIKKIGLGRRTAAAGAAAEVAAPGGAAFAAAGAARQQGGLGQTPVEMMSGWLTRMPFSAQQRALTVGVVVSLSALLASV
YLDNRQASNGAAQIEVTGDMLMHSQRLGKAVPVALLGNAQAFKQLRESKEQLTTDMLALQNGSDEKHVRATSGPGEPLLE
KAMGSWKRSEKSAADVLAQQAILTTIGQTLQIFNASNPELLEAAEQVAAIKLQSGANTREVAASAQLVMLTQRLGKNLNE
FLSGEGVNPETAFLLGKDTNTFRETLDGLINGSEALRLTAAHDAETRSYLQQLSQRFDAVQKTTQTILQNLPGLIAAKRA
QQQIFNDNEALRGELSALQSAYAQDARFRPWTLGAMVISALATLLCLAGLAALYLRDSRVRALEAEAREHEAEARRLDEK
RNNDATQKAILQLMNELQDIADGDLTRQATVTEDITGAIADSVNYTVEELKELVGRVQQTAGDVQHASTQVQETSVQLAT
TTEQQSRQIRQTGESVVEMADRITQVSRGAAESANVARASLAAAEQGQHAVQNAIAGMNGIREQIQETSKRIKRLGESSQ
EIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSGEATKQIGALIRTIQTDTQDAVHAMEQST
QGVVEGARLSDNAGAALVEIGRVSRQLAELIEQISQTTSHEAGLATNVARHIDGILQVTAQTTAGTRQTAASVRQLTALT
EELRNSVSRFKIA

Sequences:

>Translated_733_residues
MGIKKIGLGRRTAAAGAAAEVAAPGGAAFAAAGAARQQGGLGQTPVEMMSGWLTRMPFSAQQRALTVGVVVSLSALLASV
YLDNRQASNGAAQIEVTGDMLMHSQRLGKAVPVALLGNAQAFKQLRESKEQLTTDMLALQNGSDEKHVRATSGPGEPLLE
KAMGSWKRSEKSAADVLAQQAILTTIGQTLQIFNASNPELLEAAEQVAAIKLQSGANTREVAASAQLVMLTQRLGKNLNE
FLSGEGVNPETAFLLGKDTNTFRETLDGLINGSEALRLTAAHDAETRSYLQQLSQRFDAVQKTTQTILQNLPGLIAAKRA
QQQIFNDNEALRGELSALQSAYAQDARFRPWTLGAMVISALATLLCLAGLAALYLRDSRVRALEAEAREHEAEARRLDEK
RNNDATQKAILQLMNELQDIADGDLTRQATVTEDITGAIADSVNYTVEELKELVGRVQQTAGDVQHASTQVQETSVQLAT
TTEQQSRQIRQTGESVVEMADRITQVSRGAAESANVARASLAAAEQGQHAVQNAIAGMNGIREQIQETSKRIKRLGESSQ
EIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSGEATKQIGALIRTIQTDTQDAVHAMEQST
QGVVEGARLSDNAGAALVEIGRVSRQLAELIEQISQTTSHEAGLATNVARHIDGILQVTAQTTAGTRQTAASVRQLTALT
EELRNSVSRFKIA
>Mature_732_residues
GIKKIGLGRRTAAAGAAAEVAAPGGAAFAAAGAARQQGGLGQTPVEMMSGWLTRMPFSAQQRALTVGVVVSLSALLASVY
LDNRQASNGAAQIEVTGDMLMHSQRLGKAVPVALLGNAQAFKQLRESKEQLTTDMLALQNGSDEKHVRATSGPGEPLLEK
AMGSWKRSEKSAADVLAQQAILTTIGQTLQIFNASNPELLEAAEQVAAIKLQSGANTREVAASAQLVMLTQRLGKNLNEF
LSGEGVNPETAFLLGKDTNTFRETLDGLINGSEALRLTAAHDAETRSYLQQLSQRFDAVQKTTQTILQNLPGLIAAKRAQ
QQIFNDNEALRGELSALQSAYAQDARFRPWTLGAMVISALATLLCLAGLAALYLRDSRVRALEAEAREHEAEARRLDEKR
NNDATQKAILQLMNELQDIADGDLTRQATVTEDITGAIADSVNYTVEELKELVGRVQQTAGDVQHASTQVQETSVQLATT
TEQQSRQIRQTGESVVEMADRITQVSRGAAESANVARASLAAAEQGQHAVQNAIAGMNGIREQIQETSKRIKRLGESSQE
IGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSGEATKQIGALIRTIQTDTQDAVHAMEQSTQ
GVVEGARLSDNAGAALVEIGRVSRQLAELIEQISQTTSHEAGLATNVARHIDGILQVTAQTTAGTRQTAASVRQLTALTE
ELRNSVSRFKIA

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=272, Percent_Identity=29.0441176470588, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788194, Length=333, Percent_Identity=28.5285285285285, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788195, Length=323, Percent_Identity=27.2445820433437, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1787690, Length=283, Percent_Identity=31.095406360424, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1789453, Length=250, Percent_Identity=30.8, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 78097; Mature: 77966

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIKKIGLGRRTAAAGAAAEVAAPGGAAFAAAGAARQQGGLGQTPVEMMSGWLTRMPFSA
CCCCCCCCCCHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCH
QQRALTVGVVVSLSALLASVYLDNRQASNGAAQIEVTGDMLMHSQRLGKAVPVALLGNAQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHCCHH
AFKQLRESKEQLTTDMLALQNGSDEKHVRATSGPGEPLLEKAMGSWKRSEKSAADVLAQQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AILTTIGQTLQIFNASNPELLEAAEQVAAIKLQSGANTREVAASAQLVMLTQRLGKNLNE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
FLSGEGVNPETAFLLGKDTNTFRETLDGLINGSEALRLTAAHDAETRSYLQQLSQRFDAV
HHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHEEEEECCCHHHHHHHHHHHHHHHHH
QKTTQTILQNLPGLIAAKRAQQQIFNDNEALRGELSALQSAYAQDARFRPWTLGAMVISA
HHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LATLLCLAGLAALYLRDSRVRALEAEAREHEAEARRLDEKRNNDATQKAILQLMNELQDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ADGDLTRQATVTEDITGAIADSVNYTVEELKELVGRVQQTAGDVQHASTQVQETSVQLAT
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TTEQQSRQIRQTGESVVEMADRITQVSRGAAESANVARASLAAAEQGQHAVQNAIAGMNG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IREQIQETSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFSVV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHCCCCCCCCHHHH
AEEVQRLAERSGEATKQIGALIRTIQTDTQDAVHAMEQSTQGVVEGARLSDNAGAALVEI
HHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
GRVSRQLAELIEQISQTTSHEAGLATNVARHIDGILQVTAQTTAGTRQTAASVRQLTALT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EELRNSVSRFKIA
HHHHHHHHHHCCC
>Mature Secondary Structure 
GIKKIGLGRRTAAAGAAAEVAAPGGAAFAAAGAARQQGGLGQTPVEMMSGWLTRMPFSA
CCCCCCCCCHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCH
QQRALTVGVVVSLSALLASVYLDNRQASNGAAQIEVTGDMLMHSQRLGKAVPVALLGNAQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHCCHH
AFKQLRESKEQLTTDMLALQNGSDEKHVRATSGPGEPLLEKAMGSWKRSEKSAADVLAQQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AILTTIGQTLQIFNASNPELLEAAEQVAAIKLQSGANTREVAASAQLVMLTQRLGKNLNE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
FLSGEGVNPETAFLLGKDTNTFRETLDGLINGSEALRLTAAHDAETRSYLQQLSQRFDAV
HHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHEEEEECCCHHHHHHHHHHHHHHHHH
QKTTQTILQNLPGLIAAKRAQQQIFNDNEALRGELSALQSAYAQDARFRPWTLGAMVISA
HHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LATLLCLAGLAALYLRDSRVRALEAEAREHEAEARRLDEKRNNDATQKAILQLMNELQDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ADGDLTRQATVTEDITGAIADSVNYTVEELKELVGRVQQTAGDVQHASTQVQETSVQLAT
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TTEQQSRQIRQTGESVVEMADRITQVSRGAAESANVARASLAAAEQGQHAVQNAIAGMNG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IREQIQETSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFSVV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHCCCCCCCCHHHH
AEEVQRLAERSGEATKQIGALIRTIQTDTQDAVHAMEQSTQGVVEGARLSDNAGAALVEI
HHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
GRVSRQLAELIEQISQTTSHEAGLATNVARHIDGILQVTAQTTAGTRQTAASVRQLTALT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EELRNSVSRFKIA
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]