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The map label for this gene is cpaF1 [C]

Identifier: 94309586

GI number: 94309586

Start: 707667

End: 709037

Strand: Reverse

Name: cpaF1 [C]

Synonym: Rmet_0641

Alternate gene names: 94309586

Gene position: 709037-707667 (Counterclockwise)

Preceding gene: 94309587

Following gene: 94309585

Centisome position: 18.05

GC content: 63.31

Gene sequence:

>1371_bases
ATGTCAATCCGCGAACAGCTCGCCAGCGCCGGGACACCCCACACGGCAAATGGTCACGCCGCGATCCATTTCTCGATGGC
CACCAACACGACATCGCCCGGGTACCACGCGCTGAAGCGCGACGTGCACGAGACCGTGCTGGACCGCGTGGAGCTGGAGC
GCCTGGCGCACTTCCCGCAGGAACAGGTACGTCAGGAGATCGCCTCGCTGGTGAGCCTGATCATCGAGGAACATAAGGTG
CTGCTCAATGACAACGAGCGGCGACAACTCACCGTAGAGATCTATGACGAGATGTTCGGCTTCGGGCCGCTGGAACCACT
GTTGAAGGACCCCACGGTCTCGGACATCCTGGTCAACACGGCACGACAAACCTACGTGGAGCGGCGCGGCAAGCTCGAAC
TGACGAATGTGGTGTTCTATGACGACGCCCATTTGATGAAAGTCATCGAGAAGATCGTGTCGCGCGTCGGCCGCCGCATC
GACGAAACCAGCCCCATGGTGGATGCGCGCCTGCCGGACGGCTCTCGCGTTAACGCCATCATCCCGCCATCTGCCATCGA
TGGTCCACTGCTGTCGATTCGTCGCTTCTCCGTGAACCCGCTGCAGGTGTCCGACCTGGTCGAGCTGAAGAGCCTCACGC
AGCCGATGGCGCAAATGCTGCAGGCGCTGGCGCATGCCAAGATCAACGTGTTGGTCTCGGGCGGTACGGGCAGCGGGAAG
ACCACGCTGCTGAACATCCTGTCCGGATTCATCCCGGAAGACGAACGCGTGGTGACCATCGAGGATGCCGCCGAACTCCA
ACTGCGCCAGCCTCATGTCCTGCGCCTGGAAACGCGGCCGCCCAATATCGAAGGCAAGGGAGAAATCACCCAGCGCGCGC
TGGTGCGCAACGCGCTGCGGATGCGCCCGGACCGCATCATCCTCGGCGAAATCCGTGGTGGCGAGGCACTGGACATGCTC
AACGCCATGAACACCGGCCACGAGGGCTCGCTCACGACGATCCACGCCAACACCCCGCGTGATGCGCTGACGCGGCTCGA
GAACATGGTCAGCATGGCAGGCCTGACCATGCCGGCCAAGGCCATGCGCCAGCAGATCGCCTCGGCGATCACGGTGATCG
TGCAGGCGGCGCGTCTGACCGACGGGCGGCGCAAGATCATCAGCATCCAGGAAATCACTGGCATGGAAGGCGACATCATC
AACACGCAGGAGATCTTCACGTTCCAGCGCACGGGTGTGGCCGAGGATGGCACGGTCCGCGGCCACTTCAAGGCCACGGG
CGTGTATCCGAAGTTTGCGGAGCGGCTGCGCGTCTTTGGCGTCGGGCTGCCGGACGAGACCTACGATCCCGCGCGGCGCT
ACGAAGTCTGA

Upstream 100 bases:

>100_bases
CGTTACGCAAGCTGATGCTGCGCGCCCGTAGCACCTGAATCCGCAGGCCACCGATCCGTCAGAGCCACCCGAACCGAATA
CGCTTAGCAAGGAGTCCATC

Downstream 100 bases:

>100_bases
GCCCACGCACTGATCAACGACAACATATCCGGGGGGTAACACATGAGCACGATCTTCTATGCCTTCGGCATCTTGCTGTT
CGTCGCCGTGGTGCTCAGTG

Product: flp pilus assembly ATPase CpaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSIREQLASAGTPHTANGHAAIHFSMATNTTSPGYHALKRDVHETVLDRVELERLAHFPQEQVRQEIASLVSLIIEEHKV
LLNDNERRQLTVEIYDEMFGFGPLEPLLKDPTVSDILVNTARQTYVERRGKLELTNVVFYDDAHLMKVIEKIVSRVGRRI
DETSPMVDARLPDGSRVNAIIPPSAIDGPLLSIRRFSVNPLQVSDLVELKSLTQPMAQMLQALAHAKINVLVSGGTGSGK
TTLLNILSGFIPEDERVVTIEDAAELQLRQPHVLRLETRPPNIEGKGEITQRALVRNALRMRPDRIILGEIRGGEALDML
NAMNTGHEGSLTTIHANTPRDALTRLENMVSMAGLTMPAKAMRQQIASAITVIVQAARLTDGRRKIISIQEITGMEGDII
NTQEIFTFQRTGVAEDGTVRGHFKATGVYPKFAERLRVFGVGLPDETYDPARRYEV

Sequences:

>Translated_456_residues
MSIREQLASAGTPHTANGHAAIHFSMATNTTSPGYHALKRDVHETVLDRVELERLAHFPQEQVRQEIASLVSLIIEEHKV
LLNDNERRQLTVEIYDEMFGFGPLEPLLKDPTVSDILVNTARQTYVERRGKLELTNVVFYDDAHLMKVIEKIVSRVGRRI
DETSPMVDARLPDGSRVNAIIPPSAIDGPLLSIRRFSVNPLQVSDLVELKSLTQPMAQMLQALAHAKINVLVSGGTGSGK
TTLLNILSGFIPEDERVVTIEDAAELQLRQPHVLRLETRPPNIEGKGEITQRALVRNALRMRPDRIILGEIRGGEALDML
NAMNTGHEGSLTTIHANTPRDALTRLENMVSMAGLTMPAKAMRQQIASAITVIVQAARLTDGRRKIISIQEITGMEGDII
NTQEIFTFQRTGVAEDGTVRGHFKATGVYPKFAERLRVFGVGLPDETYDPARRYEV
>Mature_455_residues
SIREQLASAGTPHTANGHAAIHFSMATNTTSPGYHALKRDVHETVLDRVELERLAHFPQEQVRQEIASLVSLIIEEHKVL
LNDNERRQLTVEIYDEMFGFGPLEPLLKDPTVSDILVNTARQTYVERRGKLELTNVVFYDDAHLMKVIEKIVSRVGRRID
ETSPMVDARLPDGSRVNAIIPPSAIDGPLLSIRRFSVNPLQVSDLVELKSLTQPMAQMLQALAHAKINVLVSGGTGSGKT
TLLNILSGFIPEDERVVTIEDAAELQLRQPHVLRLETRPPNIEGKGEITQRALVRNALRMRPDRIILGEIRGGEALDMLN
AMNTGHEGSLTTIHANTPRDALTRLENMVSMAGLTMPAKAMRQQIASAITVIVQAARLTDGRRKIISIQEITGMEGDIIN
TQEIFTFQRTGVAEDGTVRGHFKATGVYPKFAERLRVFGVGLPDETYDPARRYEV

Specific function: Unknown

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=194, Percent_Identity=31.4432989690722, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 50612; Mature: 50481

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIREQLASAGTPHTANGHAAIHFSMATNTTSPGYHALKRDVHETVLDRVELERLAHFPQ
CCHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
EQVRQEIASLVSLIIEEHKVLLNDNERRQLTVEIYDEMFGFGPLEPLLKDPTVSDILVNT
HHHHHHHHHHHHHHHHHCHHEECCCCCCEEEHEEHHHHHCCCCCCHHHCCCCHHHHHHHH
ARQTYVERRGKLELTNVVFYDDAHLMKVIEKIVSRVGRRIDETSPMVDARLPDGSRVNAI
HHHHHHHHCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEE
IPPSAIDGPLLSIRRFSVNPLQVSDLVELKSLTQPMAQMLQALAHAKINVLVSGGTGSGK
ECCCCCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCH
TTLLNILSGFIPEDERVVTIEDAAELQLRQPHVLRLETRPPNIEGKGEITQRALVRNALR
HHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
MRPDRIILGEIRGGEALDMLNAMNTGHEGSLTTIHANTPRDALTRLENMVSMAGLTMPAK
CCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHH
AMRQQIASAITVIVQAARLTDGRRKIISIQEITGMEGDIINTQEIFTFQRTGVAEDGTVR
HHHHHHHHHHHHHHHHHHHCCCHHHEEEHHHHCCCCCCCCCHHHHEEECCCCCCCCCCEE
GHFKATGVYPKFAERLRVFGVGLPDETYDPARRYEV
EEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
SIREQLASAGTPHTANGHAAIHFSMATNTTSPGYHALKRDVHETVLDRVELERLAHFPQ
CHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
EQVRQEIASLVSLIIEEHKVLLNDNERRQLTVEIYDEMFGFGPLEPLLKDPTVSDILVNT
HHHHHHHHHHHHHHHHHCHHEECCCCCCEEEHEEHHHHHCCCCCCHHHCCCCHHHHHHHH
ARQTYVERRGKLELTNVVFYDDAHLMKVIEKIVSRVGRRIDETSPMVDARLPDGSRVNAI
HHHHHHHHCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEE
IPPSAIDGPLLSIRRFSVNPLQVSDLVELKSLTQPMAQMLQALAHAKINVLVSGGTGSGK
ECCCCCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCH
TTLLNILSGFIPEDERVVTIEDAAELQLRQPHVLRLETRPPNIEGKGEITQRALVRNALR
HHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
MRPDRIILGEIRGGEALDMLNAMNTGHEGSLTTIHANTPRDALTRLENMVSMAGLTMPAK
CCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHH
AMRQQIASAITVIVQAARLTDGRRKIISIQEITGMEGDIINTQEIFTFQRTGVAEDGTVR
HHHHHHHHHHHHHHHHHHHCCCHHHEEEHHHHCCCCCCCCCHHHHEEECCCCCCCCCCEE
GHFKATGVYPKFAERLRVFGVGLPDETYDPARRYEV
EEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]