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The map label for this gene is groL [H]

Identifier: 94309561

GI number: 94309561

Start: 674944

End: 676587

Strand: Direct

Name: groL [H]

Synonym: Rmet_0616

Alternate gene names: 94309561

Gene position: 674944-676587 (Clockwise)

Preceding gene: 94309560

Following gene: 94309571

Centisome position: 17.18

GC content: 62.9

Gene sequence:

>1644_bases
ATGGCAGCAAAAGACGTAGTGTTCGGCGACGCCGCCCGTGCCAAGATGGTCGAAGGCGTGAACATCCTCGCCAACGCAGT
GAAGGTGACCCTGGGCCCGAAGGGCCGCAACGTGGTGCTGGAGCGCAGCTTCGGCGGCCCGACCGTGACCAAGGACGGCG
TGTCCGTGGCCAAGGAAATCGAACTGAAGGACAAGCTGCAGAACATGGGCGCGCAGATGGTCAAGGAAGTGGCTTCCAAG
ACCAGCGACAACGCCGGTGACGGTACCACCACCGCAACCGTGCTGGCCCAGTCGATCGTGCGCGAAGGCATGAAGTTCGT
TGCCGCTGGCATGAACCCGATGGACCTGAAGCGCGGCATCGACAAGGCCGTTGGCGCCGCCGTCGAAGAGCTGAAGAAGC
TGAGCAAGCCGACGACCACCAGCAAGGAAATCGCCCAGGTTGGCGCGATCTCGGCCAACAGCGACGCCTCGATCGGCGAG
CGCATTGCCGAAGCCATGGACAAGGTTGGCAAGGAAGGCGTGATCACCGTCGAAGACGGCAAGTCGCTGGCCGACGAGCT
GGAAGTCGTGGAAGGTATGCAGTTCGACCGTGGCTACCTGTCGCCGTACTTCATCAACAACCCGGAGAAGCAGGTTGTTC
AGCTGGACAGCCCGTTCGTTCTGCTGTTCGACAAGAAGGTCTCGAACATTCGTGACCTGCTGCCGGTGCTGGAGCAAGTG
GCCAAGGCTGGCCGCCCGCTGCTGATCATCGCTGAAGACGTGGAAGGCGAAGCCCTGGCCACGCTGGTGGTGAACAACAT
CCGTGGCATCCTGAAGACCGCCGCCGTGAAGGCTCCGGGCTTCGGCGACCGCCGCAAGGCCATGCTGGAAGACATCGCCA
TCCTGACCGGCGGTACCGTGATCGCCGAGGAAATCGGTCTGACGCTGGAAAAGGCCACGCTGCAGGACCTGGGCCAAGCC
AAGCGCATCGAGATCGGCAAGGAAAACACTATCATCATCGACGGCGCCGGCGACGCATCGGCGATCGAAGGCCGCGTGAA
GCAAATCCGCGCCCAGATCGAAGAAGCGACCTCGGACTACGACCGTGAGAAGCTGCAAGAGCGCGTGGCCAAGCTGGCCG
GCGGTGTTGCCGTGATCAAGGTTGGCGCTGCCACCGAAGTCGAAATGAAGGAAAAGAAGGCACGCGTGGAAGATGCACTG
CACGCTACCCGTGCTGCCGTGGAAGAAGGCATCGTCCCGGGCGGTGGTGTGGCGCTGCTGCGTGCTCGTGCTGCGATCGC
TGGCCTGCACGGCGAGAACCCCGACCAGAACGCCGGTATCAAGATCGTGCTGCGCGCCATGGAAGAGCCGCTGCGCCAGA
TCGTGCTGAACGCTGGTGAAGAAGCTTCGGTTGTGGTGGCCAAGGTCATCGAAGGTAAGGGTAACTACGGTTACAACGCG
GCTTCGGGCGAGTACGGCGACCTGGTGGAAATGGGCGTGCTGGATCCGACCAAGGTGACCCGCACCGCGCTGCAGAACGC
CGCTTCGGTGGCTTCGCTGATGCTGACCACCGACTGCGCCGTGGCCGAATCGCCGAAGGAAGAGTCGGCTCCGGCAATGC
CGGGCGGCATGGGCGGCATGGGCGGCATGGAAGGCATGATGTAA

Upstream 100 bases:

>100_bases
TGTCGTCAACAAGTAATCGACGCCCACAAGCCACCCAATTCCCGATCGTCCCCCTGACAATCCAGCGGGACGCATCCATC
CAGATTCGGAGATTCAGAAC

Downstream 100 bases:

>100_bases
TTCATGCCGGACTGCTGAGCCAATCAGCCTGTCTGAGAAAAACCCCCGATGGCGCGAGCCCTCGGGGGTTTTTCATTGGC
CGGGTCAGATCGCGGACAAC

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MAAKDVVFGDAARAKMVEGVNILANAVKVTLGPKGRNVVLERSFGGPTVTKDGVSVAKEIELKDKLQNMGAQMVKEVASK
TSDNAGDGTTTATVLAQSIVREGMKFVAAGMNPMDLKRGIDKAVGAAVEELKKLSKPTTTSKEIAQVGAISANSDASIGE
RIAEAMDKVGKEGVITVEDGKSLADELEVVEGMQFDRGYLSPYFINNPEKQVVQLDSPFVLLFDKKVSNIRDLLPVLEQV
AKAGRPLLIIAEDVEGEALATLVVNNIRGILKTAAVKAPGFGDRRKAMLEDIAILTGGTVIAEEIGLTLEKATLQDLGQA
KRIEIGKENTIIIDGAGDASAIEGRVKQIRAQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDAL
HATRAAVEEGIVPGGGVALLRARAAIAGLHGENPDQNAGIKIVLRAMEEPLRQIVLNAGEEASVVVAKVIEGKGNYGYNA
ASGEYGDLVEMGVLDPTKVTRTALQNAASVASLMLTTDCAVAESPKEESAPAMPGGMGGMGGMEGMM

Sequences:

>Translated_547_residues
MAAKDVVFGDAARAKMVEGVNILANAVKVTLGPKGRNVVLERSFGGPTVTKDGVSVAKEIELKDKLQNMGAQMVKEVASK
TSDNAGDGTTTATVLAQSIVREGMKFVAAGMNPMDLKRGIDKAVGAAVEELKKLSKPTTTSKEIAQVGAISANSDASIGE
RIAEAMDKVGKEGVITVEDGKSLADELEVVEGMQFDRGYLSPYFINNPEKQVVQLDSPFVLLFDKKVSNIRDLLPVLEQV
AKAGRPLLIIAEDVEGEALATLVVNNIRGILKTAAVKAPGFGDRRKAMLEDIAILTGGTVIAEEIGLTLEKATLQDLGQA
KRIEIGKENTIIIDGAGDASAIEGRVKQIRAQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDAL
HATRAAVEEGIVPGGGVALLRARAAIAGLHGENPDQNAGIKIVLRAMEEPLRQIVLNAGEEASVVVAKVIEGKGNYGYNA
ASGEYGDLVEMGVLDPTKVTRTALQNAASVASLMLTTDCAVAESPKEESAPAMPGGMGGMGGMEGMM
>Mature_546_residues
AAKDVVFGDAARAKMVEGVNILANAVKVTLGPKGRNVVLERSFGGPTVTKDGVSVAKEIELKDKLQNMGAQMVKEVASKT
SDNAGDGTTTATVLAQSIVREGMKFVAAGMNPMDLKRGIDKAVGAAVEELKKLSKPTTTSKEIAQVGAISANSDASIGER
IAEAMDKVGKEGVITVEDGKSLADELEVVEGMQFDRGYLSPYFINNPEKQVVQLDSPFVLLFDKKVSNIRDLLPVLEQVA
KAGRPLLIIAEDVEGEALATLVVNNIRGILKTAAVKAPGFGDRRKAMLEDIAILTGGTVIAEEIGLTLEKATLQDLGQAK
RIEIGKENTIIIDGAGDASAIEGRVKQIRAQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALH
ATRAAVEEGIVPGGGVALLRARAAIAGLHGENPDQNAGIKIVLRAMEEPLRQIVLNAGEEASVVVAKVIEGKGNYGYNAA
SGEYGDLVEMGVLDPTKVTRTALQNAASVASLMLTTDCAVAESPKEESAPAMPGGMGGMGGMEGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=530, Percent_Identity=53.7735849056604, Blast_Score=554, Evalue=1e-158,
Organism=Homo sapiens, GI31542947, Length=530, Percent_Identity=53.7735849056604, Blast_Score=554, Evalue=1e-158,
Organism=Homo sapiens, GI24307939, Length=358, Percent_Identity=22.6256983240223, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790586, Length=530, Percent_Identity=77.3584905660377, Blast_Score=816, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=530, Percent_Identity=53.5849056603774, Blast_Score=556, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI193210679, Length=212, Percent_Identity=53.3018867924528, Blast_Score=221, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI25147750, Length=131, Percent_Identity=35.1145038167939, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=53.4220532319392, Blast_Score=556, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6322350, Length=573, Percent_Identity=22.6876090750436, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322524, Length=556, Percent_Identity=21.0431654676259, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322446, Length=570, Percent_Identity=22.280701754386, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=53.8899430740038, Blast_Score=566, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=53.8899430740038, Blast_Score=566, Evalue=1e-161,
Organism=Drosophila melanogaster, GI45550936, Length=527, Percent_Identity=51.6129032258064, Blast_Score=543, Evalue=1e-155,
Organism=Drosophila melanogaster, GI45550132, Length=527, Percent_Identity=51.6129032258064, Blast_Score=543, Evalue=1e-155,
Organism=Drosophila melanogaster, GI45550935, Length=527, Percent_Identity=51.6129032258064, Blast_Score=543, Evalue=1e-155,
Organism=Drosophila melanogaster, GI17864606, Length=542, Percent_Identity=45.5719557195572, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24584129, Length=532, Percent_Identity=37.0300751879699, Blast_Score=345, Evalue=7e-95,
Organism=Drosophila melanogaster, GI19921262, Length=532, Percent_Identity=37.0300751879699, Blast_Score=345, Evalue=7e-95,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57379; Mature: 57248

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00284 SERPIN ; PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKDVVFGDAARAKMVEGVNILANAVKVTLGPKGRNVVLERSFGGPTVTKDGVSVAKEI
CCCCCCEECCHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHH
ELKDKLQNMGAQMVKEVASKTSDNAGDGTTTATVLAQSIVREGMKFVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
DKAVGAAVEELKKLSKPTTTSKEIAQVGAISANSDASIGERIAEAMDKVGKEGVITVEDG
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCC
KSLADELEVVEGMQFDRGYLSPYFINNPEKQVVQLDSPFVLLFDKKVSNIRDLLPVLEQV
HHHHHHHHHHHCCCCCCCCCCCEEECCCHHHEEEECCCEEEEECCHHHHHHHHHHHHHHH
AKAGRPLLIIAEDVEGEALATLVVNNIRGILKTAAVKAPGFGDRRKAMLEDIAILTGGTV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHH
IAEEIGLTLEKATLQDLGQAKRIEIGKENTIIIDGAGDASAIEGRVKQIRAQIEEATSDY
HHHHHCCHHHHHHHHHCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
DREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RARAAIAGLHGENPDQNAGIKIVLRAMEEPLRQIVLNAGEEASVVVAKVIEGKGNYGYNA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
ASGEYGDLVEMGVLDPTKVTRTALQNAASVASLMLTTDCAVAESPKEESAPAMPGGMGGM
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCC
GGMEGMM
CCCCCCC
>Mature Secondary Structure 
AAKDVVFGDAARAKMVEGVNILANAVKVTLGPKGRNVVLERSFGGPTVTKDGVSVAKEI
CCCCCEECCHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHH
ELKDKLQNMGAQMVKEVASKTSDNAGDGTTTATVLAQSIVREGMKFVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
DKAVGAAVEELKKLSKPTTTSKEIAQVGAISANSDASIGERIAEAMDKVGKEGVITVEDG
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCC
KSLADELEVVEGMQFDRGYLSPYFINNPEKQVVQLDSPFVLLFDKKVSNIRDLLPVLEQV
HHHHHHHHHHHCCCCCCCCCCCEEECCCHHHEEEECCCEEEEECCHHHHHHHHHHHHHHH
AKAGRPLLIIAEDVEGEALATLVVNNIRGILKTAAVKAPGFGDRRKAMLEDIAILTGGTV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHH
IAEEIGLTLEKATLQDLGQAKRIEIGKENTIIIDGAGDASAIEGRVKQIRAQIEEATSDY
HHHHHCCHHHHHHHHHCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
DREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RARAAIAGLHGENPDQNAGIKIVLRAMEEPLRQIVLNAGEEASVVVAKVIEGKGNYGYNA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
ASGEYGDLVEMGVLDPTKVTRTALQNAASVASLMLTTDCAVAESPKEESAPAMPGGMGGM
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCC
GGMEGMM
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA