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The map label for this gene is kdsB [H]

Identifier: 94309478

GI number: 94309478

Start: 568475

End: 569284

Strand: Reverse

Name: kdsB [H]

Synonym: Rmet_0533

Alternate gene names: 94309478

Gene position: 569284-568475 (Counterclockwise)

Preceding gene: 94309479

Following gene: 94309477

Centisome position: 14.49

GC content: 69.14

Gene sequence:

>810_bases
ATGAGCATCCCGACCTTTACCGTTGTCATCCCTGCCCGCCTCGCCTCCACGCGGCTGCCAAACAAGCCGCTGGCCGACAT
CGGCGGCCATCCGATGATCGTGCGCGTGGCTGAACGCGCCCACGCGTCGTCGGCCCAGCGCACCGTGGTGGCAACGGACT
CCCAGGAAGTTGCGCAGGCCTGCGCCGCGCATGGCGTCGAAGCCGTGCTCACGCGTGCCGACCATCAGTCCGGCACCGAC
CGGCTGTCGGAAGTGGCGGCACAGCTCGGGCTGGCCGATGACGCGATCGTCGTCAACGTACAGGGCGACGAGCCGCTGAT
CGAGCCGACGCTGATCGACGAAGTCGCGCTGCATCTGGCGGCTCACACTGACTGCGCGATCGCCACGGCAGCGCATCCGC
TGGACGACGTGGCCGAAGTATTCAACCCGAACGTGGTGAAAGTGGTCTGCGACAACGCGGGCCGCGCGCTCTATTTCTCG
CGCGCGCCGATTCCGTGGGCACGCGATGCCTGGTCTGCCGTGCCGGCCCAGCCCGCGGCCACCGCGCAAGTCCCGCTGCC
GGCCATGCCGGTGCTGCGTCACATCGGCATCTATGCCTATCGCGCGGGCTTCCTGCGCCGCTTCCCGACGTTGGCGGTCG
CGCCGGTCGAACAGACCGAAGCGCTCGAGCAACTGCGTGCCATGTGGCATGGCGAGCGGATCGGCGTCATGGTGACGGCG
GCTGCACCGGCGCCGGGCGTGGACACACCGGCGGACCTGGAACGCGTGCGTGCGCTATGGGTGCAGGGCATGGCTCAGGA
AGGGCCGTGA

Upstream 100 bases:

>100_bases
CGGTCATGCTGGCGGACGAAGCGCGCCAGACCGTGCCGGGCCGTGTGGTACCGGTGGAACCCTCGGCGCCGGCCGGCAAT
TGATGGGCAAAGGTCGCACG

Downstream 100 bases:

>100_bases
CAACATTCGCCCGCTGAAGTTCACACTGCTGCAACTTCGGCGGGGGCCGAATCCGTACGGCCCATGGCATAATGCTTGCC
GAAACAGAGTCCGTGAGGCG

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSIPTFTVVIPARLASTRLPNKPLADIGGHPMIVRVAERAHASSAQRTVVATDSQEVAQACAAHGVEAVLTRADHQSGTD
RLSEVAAQLGLADDAIVVNVQGDEPLIEPTLIDEVALHLAAHTDCAIATAAHPLDDVAEVFNPNVVKVVCDNAGRALYFS
RAPIPWARDAWSAVPAQPAATAQVPLPAMPVLRHIGIYAYRAGFLRRFPTLAVAPVEQTEALEQLRAMWHGERIGVMVTA
AAPAPGVDTPADLERVRALWVQGMAQEGP

Sequences:

>Translated_269_residues
MSIPTFTVVIPARLASTRLPNKPLADIGGHPMIVRVAERAHASSAQRTVVATDSQEVAQACAAHGVEAVLTRADHQSGTD
RLSEVAAQLGLADDAIVVNVQGDEPLIEPTLIDEVALHLAAHTDCAIATAAHPLDDVAEVFNPNVVKVVCDNAGRALYFS
RAPIPWARDAWSAVPAQPAATAQVPLPAMPVLRHIGIYAYRAGFLRRFPTLAVAPVEQTEALEQLRAMWHGERIGVMVTA
AAPAPGVDTPADLERVRALWVQGMAQEGP
>Mature_268_residues
SIPTFTVVIPARLASTRLPNKPLADIGGHPMIVRVAERAHASSAQRTVVATDSQEVAQACAAHGVEAVLTRADHQSGTDR
LSEVAAQLGLADDAIVVNVQGDEPLIEPTLIDEVALHLAAHTDCAIATAAHPLDDVAEVFNPNVVKVVCDNAGRALYFSR
APIPWARDAWSAVPAQPAATAQVPLPAMPVLRHIGIYAYRAGFLRRFPTLAVAPVEQTEALEQLRAMWHGERIGVMVTAA
APAPGVDTPADLERVRALWVQGMAQEGP

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria [H]

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family [H]

Homologues:

Organism=Escherichia coli, GI1787147, Length=254, Percent_Identity=54.3307086614173, Blast_Score=262, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003329
- InterPro:   IPR004528 [H]

Pfam domain/function: PF02348 CTP_transf_3 [H]

EC number: =2.7.7.38 [H]

Molecular weight: Translated: 28527; Mature: 28396

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPTFTVVIPARLASTRLPNKPLADIGGHPMIVRVAERAHASSAQRTVVATDSQEVAQA
CCCCEEEEEECHHHHHCCCCCCCHHHCCCCCEEEEEHHHHHCCCCCEEEEECCHHHHHHH
CAAHGVEAVLTRADHQSGTDRLSEVAAQLGLADDAIVVNVQGDEPLIEPTLIDEVALHLA
HHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
AHTDCAIATAAHPLDDVAEVFNPNVVKVVCDNAGRALYFSRAPIPWARDAWSAVPAQPAA
HCCCHHHHHHCCCHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCHHHHCCCCCCCC
TAQVPLPAMPVLRHIGIYAYRAGFLRRFPTLAVAPVEQTEALEQLRAMWHGERIGVMVTA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCEEEEEEEE
AAPAPGVDTPADLERVRALWVQGMAQEGP
CCCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIPTFTVVIPARLASTRLPNKPLADIGGHPMIVRVAERAHASSAQRTVVATDSQEVAQA
CCCEEEEEECHHHHHCCCCCCCHHHCCCCCEEEEEHHHHHCCCCCEEEEECCHHHHHHH
CAAHGVEAVLTRADHQSGTDRLSEVAAQLGLADDAIVVNVQGDEPLIEPTLIDEVALHLA
HHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
AHTDCAIATAAHPLDDVAEVFNPNVVKVVCDNAGRALYFSRAPIPWARDAWSAVPAQPAA
HCCCHHHHHHCCCHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCHHHHCCCCCCCC
TAQVPLPAMPVLRHIGIYAYRAGFLRRFPTLAVAPVEQTEALEQLRAMWHGERIGVMVTA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCEEEEEEEE
AAPAPGVDTPADLERVRALWVQGMAQEGP
CCCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA