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The map label for this gene is fabG2 [H]

Identifier: 94309476

GI number: 94309476

Start: 566573

End: 567331

Strand: Reverse

Name: fabG2 [H]

Synonym: Rmet_0531

Alternate gene names: 94309476

Gene position: 567331-566573 (Counterclockwise)

Preceding gene: 94309477

Following gene: 94309474

Centisome position: 14.44

GC content: 66.93

Gene sequence:

>759_bases
ATGGACATTCAGGGCAACGTATTCATTGTCACGGGCGGCGCATCCGGGCTCGGCGCGGGCACGGCGCGCATGCTGGCGGC
TGCGGGCGGCAAGGTCGTGATTGCCGACCTCAACGAAGCCGCGGGCACGGCGCTGGCCACGGAGCTCGGCGGGCAGTTCG
TCAAATGCGACGTGGCGTCGGAGGCCGACGGTCAGGCGGCCGTGGATGCCGCGCGGAAGCTTGGCCGGCTCTCCGGGCTG
GTCAACTGCGCGGGTATCGCCGTGGCCGCCAAGACGGTCGGCAAGAACGGACCGCATCCGCTCGATGCGTTCGAGAAGAC
CATCCGCGTCAACCTGATCGGCACCTTCAACATGATTCGCCTCGCCGCCGCGGAGATGGTCCAGAACACTCCCGATGCCG
AAGGCGAACGCGGCGTGATCATCAATACGGCCTCGGTGGCCGCGTTCGATGGCCAGATCGGCCAGGCTGCGTATGCGGCA
TCGAAGGGCGGCGTGGTGGGCATGACGCTGGCCATCGCGCGCGATCTTTCCCGCGACGGCGTGCGCTGCCTGACCATCGC
CCCGGGGTTGTTCGAAACACCGATGCTGCTCGGGATGCCGCCAGAGGTGCAAGAGGCGCTCGGCAAGATGGTGCCGTTCC
CGCCGCGCCTGGGCCGCCCCGCGGAGTACGCCAAGCTGGTCCAGTCGATCATCGGCAATACGATGTTGAATGGCGAAGTG
ATCCGCCTGGATGGTGCAATCCGCATGCAGCCGAAGTAA

Upstream 100 bases:

>100_bases
ACCGGGCCGCTTTTTTATTTGCCGATCGGTTCGCGCGGTTCGTACAATAGCCTCCAATTGACGTTAACGTAAACGGCATT
CACAACGAGGAGACAGGCGC

Downstream 100 bases:

>100_bases
GCCCGCGCGTCGCGCGATGCAGGGCGACTCGCGGCGAATCGCGCCGAATCAGGGTGTTTCCGGCAGCAGCATCACCAGCA
TCATCCCCGGTGCAGCCGGG

Product: Short-chain alcohol dehydrogenase/3-hydroxyacyl-CoA dehydrogenase

Products: 3-oxoacyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MDIQGNVFIVTGGASGLGAGTARMLAAAGGKVVIADLNEAAGTALATELGGQFVKCDVASEADGQAAVDAARKLGRLSGL
VNCAGIAVAAKTVGKNGPHPLDAFEKTIRVNLIGTFNMIRLAAAEMVQNTPDAEGERGVIINTASVAAFDGQIGQAAYAA
SKGGVVGMTLAIARDLSRDGVRCLTIAPGLFETPMLLGMPPEVQEALGKMVPFPPRLGRPAEYAKLVQSIIGNTMLNGEV
IRLDGAIRMQPK

Sequences:

>Translated_252_residues
MDIQGNVFIVTGGASGLGAGTARMLAAAGGKVVIADLNEAAGTALATELGGQFVKCDVASEADGQAAVDAARKLGRLSGL
VNCAGIAVAAKTVGKNGPHPLDAFEKTIRVNLIGTFNMIRLAAAEMVQNTPDAEGERGVIINTASVAAFDGQIGQAAYAA
SKGGVVGMTLAIARDLSRDGVRCLTIAPGLFETPMLLGMPPEVQEALGKMVPFPPRLGRPAEYAKLVQSIIGNTMLNGEV
IRLDGAIRMQPK
>Mature_252_residues
MDIQGNVFIVTGGASGLGAGTARMLAAAGGKVVIADLNEAAGTALATELGGQFVKCDVASEADGQAAVDAARKLGRLSGL
VNCAGIAVAAKTVGKNGPHPLDAFEKTIRVNLIGTFNMIRLAAAEMVQNTPDAEGERGVIINTASVAAFDGQIGQAAYAA
SKGGVVGMTLAIARDLSRDGVRCLTIAPGLFETPMLLGMPPEVQEALGKMVPFPPRLGRPAEYAKLVQSIIGNTMLNGEV
IRLDGAIRMQPK

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=255, Percent_Identity=59.2156862745098, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI83715985, Length=256, Percent_Identity=57.421875, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI15277342, Length=233, Percent_Identity=36.0515021459227, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI40254992, Length=248, Percent_Identity=31.0483870967742, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI31542939, Length=265, Percent_Identity=31.3207547169811, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI59889578, Length=215, Percent_Identity=32.5581395348837, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI224922801, Length=184, Percent_Identity=35.3260869565217, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4504505, Length=220, Percent_Identity=29.5454545454545, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787905, Length=236, Percent_Identity=32.2033898305085, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787335, Length=254, Percent_Identity=27.5590551181102, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082100, Length=238, Percent_Identity=27.7310924369748, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789057, Length=212, Percent_Identity=28.7735849056604, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789208, Length=255, Percent_Identity=28.6274509803922, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786812, Length=241, Percent_Identity=31.1203319502075, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI87082160, Length=256, Percent_Identity=28.515625, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788459, Length=249, Percent_Identity=28.9156626506024, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI2367365, Length=253, Percent_Identity=28.8537549407115, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790717, Length=260, Percent_Identity=26.5384615384615, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=249, Percent_Identity=53.4136546184739, Blast_Score=248, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI25147288, Length=252, Percent_Identity=31.3492063492063, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17555706, Length=223, Percent_Identity=34.9775784753363, Blast_Score=106, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17563726, Length=241, Percent_Identity=26.5560165975104, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=218, Percent_Identity=29.3577981651376, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=261, Percent_Identity=28.3524904214559, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=214, Percent_Identity=28.5046728971963, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17531453, Length=232, Percent_Identity=31.4655172413793, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560150, Length=245, Percent_Identity=26.1224489795918, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17567345, Length=204, Percent_Identity=27.4509803921569, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=259, Percent_Identity=27.7992277992278, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=232, Percent_Identity=29.7413793103448, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320089, Length=203, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322227, Length=228, Percent_Identity=25.8771929824561, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17737361, Length=254, Percent_Identity=58.2677165354331, Blast_Score=279, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24639444, Length=254, Percent_Identity=31.496062992126, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357041, Length=239, Percent_Identity=30.9623430962343, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571526, Length=242, Percent_Identity=31.404958677686, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24644339, Length=239, Percent_Identity=28.4518828451883, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665243, Length=211, Percent_Identity=27.9620853080569, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI221331218, Length=211, Percent_Identity=27.9620853080569, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI23397609, Length=200, Percent_Identity=31.5, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.35

Molecular weight: Translated: 25752; Mature: 25752

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIQGNVFIVTGGASGLGAGTARMLAAAGGKVVIADLNEAAGTALATELGGQFVKCDVAS
CCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCEEEEEECCC
EADGQAAVDAARKLGRLSGLVNCAGIAVAAKTVGKNGPHPLDAFEKTIRVNLIGTFNMIR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEEEEEEEEHHHHHH
LAAAEMVQNTPDAEGERGVIINTASVAAFDGQIGQAAYAASKGGVVGMTLAIARDLSRDG
HHHHHHHHCCCCCCCCCEEEEEECEEEEECCCCCHHHHHHCCCCEEEHHHHHHHHHCCCC
VRCLTIAPGLFETPMLLGMPPEVQEALGKMVPFPPRLGRPAEYAKLVQSIIGNTMLNGEV
CEEEEECCCCHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEE
IRLDGAIRMQPK
EEECCEEEECCC
>Mature Secondary Structure
MDIQGNVFIVTGGASGLGAGTARMLAAAGGKVVIADLNEAAGTALATELGGQFVKCDVAS
CCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCEEEEEECCC
EADGQAAVDAARKLGRLSGLVNCAGIAVAAKTVGKNGPHPLDAFEKTIRVNLIGTFNMIR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEEEEEEEEHHHHHH
LAAAEMVQNTPDAEGERGVIINTASVAAFDGQIGQAAYAASKGGVVGMTLAIARDLSRDG
HHHHHHHHCCCCCCCCCEEEEEECEEEEECCCCCHHHHHHCCCCEEEHHHHHHHHHCCCC
VRCLTIAPGLFETPMLLGMPPEVQEALGKMVPFPPRLGRPAEYAKLVQSIIGNTMLNGEV
CEEEEECCCCHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEE
IRLDGAIRMQPK
EEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-3-hydroxyacyl-CoA; NAD+

Specific reaction: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]