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The map label for this gene is yafE [H]

Identifier: 94309474

GI number: 94309474

Start: 564835

End: 565605

Strand: Reverse

Name: yafE [H]

Synonym: Rmet_0529

Alternate gene names: 94309474

Gene position: 565605-564835 (Counterclockwise)

Preceding gene: 94309476

Following gene: 94309472

Centisome position: 14.4

GC content: 64.72

Gene sequence:

>771_bases
ATGCAACAACAGGAACTCGTCGCCGCCCAGTTCGGCGCCACCGCCAATGCCTACCTGACCAGTGCTGTCCATGCTCAAGG
CGCCGATCTCGAACAACTCAAGCAAGCGCTGGCTTACCTGAAGCCCGTGGCGCATAGCCGCGTGCTCGACCTGGGCTGCG
GCGCAGGCCACGCGAGCTTTGCTGCTGCAACGGTTGCCAACGAAGTGGTGGCTTACGACCTGTCGGCGGCAATGCTCGAA
GTCGTGGCAGGTGCGGCACGCGATCGCGGCCTGGGCAACGTCAAGACGCAGCAGGGTGCCGCCGAGCAACTGCCGTTCGA
CGACGCCAGCTTCTGCGCCGTGGTCTCACGCATGAGTGCCCACCACTGGCGCGACGTCCCCGCCGCGCTGCGTGAAATCC
AGCGCGTGCTGAAGCCGAACGGCAAGGTAGTGCTGATCGACATCGTCGGCGCCGAGGACCCGCTTTGCGATACATGGCTG
CAATCCGTGGAATTGCTGCGCGACCCATCGCACATCCGCGACTACACGCCGACCGTGTGGACGCAAATGTTCGAGGCAGC
CGGATTCCAGGTAGAGGTGTCGCCGGTCTGGCGCATCGACATCGAATTCACCAGCTGGGTGGCCCGGATGCGCACGCCAG
AAGTTGCGGCAGCCGCGATCCGCCACCTGTGGGAAAAGGCGCCAGCCGAAGTTTGTGATCGCTATCGCGTGCAGCCCGAC
GGCAGCTTTGCGCTGGAAGCGGCAATGTTGGCCGCCTCTCCCCGCGCGTAA

Upstream 100 bases:

>100_bases
AAATCTGTGACTTGTACCGGGAAAAGACCGCATCTACAGTACGTGAACCCCCGGGCCCGGGCAATCCCCATCGCCAATCC
GACAAGAATCGAGGCACCCC

Downstream 100 bases:

>100_bases
CAGCCCTCCCCTCAGCCCTCCCCTCGCCCGTCGCAGTCAGCCGGTCGCGGTTTCGGCCGGGATCGGCATGGTCTGGACCA
TGTTCCAGACCGCAGCCGCC

Product: putative S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MQQQELVAAQFGATANAYLTSAVHAQGADLEQLKQALAYLKPVAHSRVLDLGCGAGHASFAAATVANEVVAYDLSAAMLE
VVAGAARDRGLGNVKTQQGAAEQLPFDDASFCAVVSRMSAHHWRDVPAALREIQRVLKPNGKVVLIDIVGAEDPLCDTWL
QSVELLRDPSHIRDYTPTVWTQMFEAAGFQVEVSPVWRIDIEFTSWVARMRTPEVAAAAIRHLWEKAPAEVCDRYRVQPD
GSFALEAAMLAASPRA

Sequences:

>Translated_256_residues
MQQQELVAAQFGATANAYLTSAVHAQGADLEQLKQALAYLKPVAHSRVLDLGCGAGHASFAAATVANEVVAYDLSAAMLE
VVAGAARDRGLGNVKTQQGAAEQLPFDDASFCAVVSRMSAHHWRDVPAALREIQRVLKPNGKVVLIDIVGAEDPLCDTWL
QSVELLRDPSHIRDYTPTVWTQMFEAAGFQVEVSPVWRIDIEFTSWVARMRTPEVAAAAIRHLWEKAPAEVCDRYRVQPD
GSFALEAAMLAASPRA
>Mature_256_residues
MQQQELVAAQFGATANAYLTSAVHAQGADLEQLKQALAYLKPVAHSRVLDLGCGAGHASFAAATVANEVVAYDLSAAMLE
VVAGAARDRGLGNVKTQQGAAEQLPFDDASFCAVVSRMSAHHWRDVPAALREIQRVLKPNGKVVLIDIVGAEDPLCDTWL
QSVELLRDPSHIRDYTPTVWTQMFEAAGFQVEVSPVWRIDIEFTSWVARMRTPEVAAAAIRHLWEKAPAEVCDRYRVQPD
GSFALEAAMLAASPRA

Specific function: Unknown

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786404, Length=201, Percent_Identity=47.2636815920398, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: NA

Molecular weight: Translated: 27777; Mature: 27777

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQELVAAQFGATANAYLTSAVHAQGADLEQLKQALAYLKPVAHSRVLDLGCGAGHASF
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AAATVANEVVAYDLSAAMLEVVAGAARDRGLGNVKTQQGAAEQLPFDDASFCAVVSRMSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHH
HHWRDVPAALREIQRVLKPNGKVVLIDIVGAEDPLCDTWLQSVELLRDPSHIRDYTPTVW
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHCCCHHHH
TQMFEAAGFQVEVSPVWRIDIEFTSWVARMRTPEVAAAAIRHLWEKAPAEVCDRYRVQPD
HHHHHHCCCEEEECEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHCCCCCC
GSFALEAAMLAASPRA
CCHHHHHHHHHCCCCC
>Mature Secondary Structure
MQQQELVAAQFGATANAYLTSAVHAQGADLEQLKQALAYLKPVAHSRVLDLGCGAGHASF
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AAATVANEVVAYDLSAAMLEVVAGAARDRGLGNVKTQQGAAEQLPFDDASFCAVVSRMSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHH
HHWRDVPAALREIQRVLKPNGKVVLIDIVGAEDPLCDTWLQSVELLRDPSHIRDYTPTVW
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHCCCHHHH
TQMFEAAGFQVEVSPVWRIDIEFTSWVARMRTPEVAAAAIRHLWEKAPAEVCDRYRVQPD
HHHHHHCCCEEEECEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHCCCCCC
GSFALEAAMLAASPRA
CCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1663890; 9278503 [H]