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The map label for this gene is proP [H]

Identifier: 94309407

GI number: 94309407

Start: 490734

End: 492431

Strand: Reverse

Name: proP [H]

Synonym: Rmet_0462

Alternate gene names: 94309407

Gene position: 492431-490734 (Counterclockwise)

Preceding gene: 94309408

Following gene: 94309406

Centisome position: 12.54

GC content: 62.49

Gene sequence:

>1698_bases
ATGGCAAACGCGCAAAACGTGGCAATGCCTGGCGGCAAAGCCCACAATGCGCCGATGACGCGCGAGGAAAAGAAGGTCAT
TCTGGCCTCGTCGCTCGGCACGGTGTTCGAGTGGTACGACTTTTATCTATACGGTTCGCTGGCGGCGGTGATCGCCAAGC
AGTTCTTCGCGGGCCTCGACCCCACTTCCGCATTTATCTTCGCCCTGCTGGCGTTCGCGGCAGGCTTCATCGTGCGTCCG
TTCGGCGCACTGGTGTTCGGCCGTCTCGGCGACATGATCGGCCGCAAGTACACCTTCCTGGTGACAATCCTGATCATGGG
CTTGTCGACATTCATCGTCGGCCTATTGCCCGGCTACGCCACGATCGGCTGGGCAGCACCGATCATCCTGATTGCACTGC
GTATGTTGCAGGGCCTGGCGCTCGGCGGCGAGTACGGCGGCGCGGCCACCTACGTGGCTGAACACGCCCCGCACGGCAAG
CGCGGCGCCTACACCGCCTGGATCCAGACCACCGCCACACTGGGCCTGTTCCTGTCCCTGATCGTCATTCTGCTGTGCCG
TGAACTCACGGGCCCGCAGTTCGACACGTGGGGCTGGCGCATCCCGTTCCTGGTCTCGATCCTGCTGCTGGCCATGTCCG
TGTATATCCGCCTGTCGATGAGCGAGTCGCCGGCCTTCCAGAAGATGAAGGCCGAGGGCAAGACCTCGAAGGCACCGCTG
ACCGAGGCATTCGGTCAATGGCGCAACCTGAAGATCGTGATCCTGGCGCTGGTAGGCCTGACCGCCGGCCAGGCCGTGGT
CTGGTACACCGGCCAGTTCTACTCGCTGTTCTTCCTGACGCAAGTGCTGAAGGTGGACGCCAAGACGGCCAACCTGCTGA
TCGCCGGCGCACTGGTGATTGGTACGCCGTTCTTCATCTTCTTCGGCTCGCTGTCGGACAAGATCGGCCGCAAATGGATC
ATCATGCTGGGCTGCGCGCTGGCCGTGCTGACCTACTTCCCGCTGTTCAAGGCGATGACCCACTACGCCAACCCGGCGCT
GGAACGTGCGCAGCAGACCGCGCAGATCGTGGTCACGGCCGATCCGAAGACCTGCTCGTTCCAGGGCAGCCCGATCGCTC
GCGAAATCGACTTCCGCAGCTCATGCGATATCGTCAAGCGCACGTTGGCCCAGGCATCGGCCAGCTATGAAGTGGTGCAG
GCCCCGGCCGGCACGATGGCTTCGGTGAAGATCGGCGACAAGGAAATCAAGGCCTTCGACGCCACGGTGACCGGCGGCCA
CAGCTTTGACGACGCCAGCAAGAAGCAGATCGCGGCGTTCAAGAAGGAAGTGAGCGAGTCCATGAGCGCGGCCGGCTATC
CGACCAAGGCAGACCCGGCGCAGATGAACACGATCATGGTGCTGGTGGTCCTGGTGATCCTGGTGATCTACGTGACCATG
GTCTACGGCCCGATCGCCGCGATGCTGGTGGAAATGTTCCCGACCCGCATCCGGTATTCGTCGATGTCGCTGCCGTATCA
CATCGGCAACGGCTGGTTCGGTGGCCTGCTGCCAACGATCTCCTTCGCCCTGGTGGCGCAGAACGGCAACATCTACTACG
GCCTCTGGTACCCGATCATCATCGCGGCCATGACCTTCGTGCTGGGCGCGCTGTTCGTCCGCGAGACCAAGGACGTGGAT
ATCTACGCGAACGACTGA

Upstream 100 bases:

>100_bases
AATCCTTTGCAGCTTCGGTGCAGAGGCCGGCACGCGCGGATACCTCAAGCGCAAGACACGGCCAGACCGGGGACCCTTTC
GACAGACGGAGGAGACAACA

Downstream 100 bases:

>100_bases
TCGCCAGTCGGTGAACCTTCGCTCAGGATGGCCAGCCGGCAGGCTGGTCTCCGCTTCCCAGGCAGGCCTTGCGCCTGCCG
TTTTTTTTGGCAGCCACCGA

Product: major facilitator superfamily permease

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MANAQNVAMPGGKAHNAPMTREEKKVILASSLGTVFEWYDFYLYGSLAAVIAKQFFAGLDPTSAFIFALLAFAAGFIVRP
FGALVFGRLGDMIGRKYTFLVTILIMGLSTFIVGLLPGYATIGWAAPIILIALRMLQGLALGGEYGGAATYVAEHAPHGK
RGAYTAWIQTTATLGLFLSLIVILLCRELTGPQFDTWGWRIPFLVSILLLAMSVYIRLSMSESPAFQKMKAEGKTSKAPL
TEAFGQWRNLKIVILALVGLTAGQAVVWYTGQFYSLFFLTQVLKVDAKTANLLIAGALVIGTPFFIFFGSLSDKIGRKWI
IMLGCALAVLTYFPLFKAMTHYANPALERAQQTAQIVVTADPKTCSFQGSPIAREIDFRSSCDIVKRTLAQASASYEVVQ
APAGTMASVKIGDKEIKAFDATVTGGHSFDDASKKQIAAFKKEVSESMSAAGYPTKADPAQMNTIMVLVVLVILVIYVTM
VYGPIAAMLVEMFPTRIRYSSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYYGLWYPIIIAAMTFVLGALFVRETKDVD
IYAND

Sequences:

>Translated_565_residues
MANAQNVAMPGGKAHNAPMTREEKKVILASSLGTVFEWYDFYLYGSLAAVIAKQFFAGLDPTSAFIFALLAFAAGFIVRP
FGALVFGRLGDMIGRKYTFLVTILIMGLSTFIVGLLPGYATIGWAAPIILIALRMLQGLALGGEYGGAATYVAEHAPHGK
RGAYTAWIQTTATLGLFLSLIVILLCRELTGPQFDTWGWRIPFLVSILLLAMSVYIRLSMSESPAFQKMKAEGKTSKAPL
TEAFGQWRNLKIVILALVGLTAGQAVVWYTGQFYSLFFLTQVLKVDAKTANLLIAGALVIGTPFFIFFGSLSDKIGRKWI
IMLGCALAVLTYFPLFKAMTHYANPALERAQQTAQIVVTADPKTCSFQGSPIAREIDFRSSCDIVKRTLAQASASYEVVQ
APAGTMASVKIGDKEIKAFDATVTGGHSFDDASKKQIAAFKKEVSESMSAAGYPTKADPAQMNTIMVLVVLVILVIYVTM
VYGPIAAMLVEMFPTRIRYSSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYYGLWYPIIIAAMTFVLGALFVRETKDVD
IYAND
>Mature_564_residues
ANAQNVAMPGGKAHNAPMTREEKKVILASSLGTVFEWYDFYLYGSLAAVIAKQFFAGLDPTSAFIFALLAFAAGFIVRPF
GALVFGRLGDMIGRKYTFLVTILIMGLSTFIVGLLPGYATIGWAAPIILIALRMLQGLALGGEYGGAATYVAEHAPHGKR
GAYTAWIQTTATLGLFLSLIVILLCRELTGPQFDTWGWRIPFLVSILLLAMSVYIRLSMSESPAFQKMKAEGKTSKAPLT
EAFGQWRNLKIVILALVGLTAGQAVVWYTGQFYSLFFLTQVLKVDAKTANLLIAGALVIGTPFFIFFGSLSDKIGRKWII
MLGCALAVLTYFPLFKAMTHYANPALERAQQTAQIVVTADPKTCSFQGSPIAREIDFRSSCDIVKRTLAQASASYEVVQA
PAGTMASVKIGDKEIKAFDATVTGGHSFDDASKKQIAAFKKEVSESMSAAGYPTKADPAQMNTIMVLVVLVILVIYVTMV
YGPIAAMLVEMFPTRIRYSSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYYGLWYPIIIAAMTFVLGALFVRETKDVDI
YAND

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=325, Percent_Identity=37.5384615384615, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1788292, Length=327, Percent_Identity=36.085626911315, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789941, Length=333, Percent_Identity=36.9369369369369, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1788942, Length=327, Percent_Identity=32.4159021406728, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI87082231, Length=222, Percent_Identity=27.4774774774775, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87082404, Length=327, Percent_Identity=25.0764525993884, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 61379; Mature: 61248

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
ANAQNVAMPGGKAHNAPMTREEKKVILASSLGTVFEWYDFYLYGSLAAVIAKQFFAGLD
CCCCCCCCCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC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HHHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]