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The map label for this gene is miaB [H]

Identifier: 94309398

GI number: 94309398

Start: 480337

End: 481689

Strand: Reverse

Name: miaB [H]

Synonym: Rmet_0453

Alternate gene names: 94309398

Gene position: 481689-480337 (Counterclockwise)

Preceding gene: 94309399

Following gene: 94309397

Centisome position: 12.26

GC content: 64.38

Gene sequence:

>1353_bases
ATGAAGAAGGTATTTGTCAAAACGTACGGCTGCCAGATGAACGAGTACGACTCGGACAAGATGGTAGACGTGCTGAACGC
GACGCAGGGCATGGAGCCCACGGACAACGTCGAAGACGCCGACGTGATCCTGTTCAACACCTGCTCGATCCGCGAAAAGG
CCCAGGAGAAAGTCTTCTCCGAACTGGGCCGCATGAAGGCGCTGAAGGCTGCCAAACCCGATCTGGTGATCGGCGTGGGT
GGCTGCGTGGCCAGCCAGGAAGGCGCCTCGATCGTGTCGCGCGCGCCGTATGTCGACGTCGTGTTCGGCCCGCAGACACT
GCACCGCCTGCCCGACCTGATCAACCGCCGCCAGCAGACCGGCCTGTCGCAGGTCGACATCTCGTTCCCGGAGATCGAGA
AGTTCGACCACCTGCCGCCGGCGCGTGTGGACGGCCCGAGCGCGTTCGTGTCGATCATGGAAGGCTGCTCGAAGTACTGC
AGCTATTGCGTGGTGCCGTACACCCGCGGCGAGGAAGTGTCCCGCCCGTTCGAGGATGTGCTGGCCGAAGTGGCCGGGCT
GGCCGAACAGGGCGTGCGCGAAGTGACGCTGCTGGGCCAGAACGTCAACGCCTATCGCGGCGCGATGGGCGGCACAAGCG
AGATCGCCGACTTCGCGCTGCTGATCGAGTACGTGGCCGATATCCCCGGCATCGAGCGCATCCGCTACACCACCAGCCAC
CCGAAGGAATTCACGTCGCGCCTGATCGAGCTCTACGGCCGTTGCGACAAGCTGGTGAACCATCTGCACCTGCCCGTGCA
GCACGCGTCCGATCGCGTGCTGGCAGGCATGAAGCGCGGTTACAGCGTGCTGGAGTACAAGAGCATCATCCGCCGGCTGC
GCGCGTTGCGTCCGGACATGTCGATGTCGTCGGACTTCATCGTCGGCTTCCCCGGCGAGACCGATGCCGACTTCGACAAG
CTGATGGCCATGGTTCACGAGATCGGCTACGACACCTCGTTCTCGTTCATCTTCAGCCCACGCCCCGGCACGCCGGCGGC
GAACCTGCACGATGACACGCCGCACGAGGTCAAGCTGCGCCGCCTGCAACTGCTGCAGGCCACGATCGAGGAGAACGTGC
AGCGCATCAGCCAGAGTATGGTCGGCACGGTTCAACGCATCCTGGTGGAAGGCCCGGCGCGCAAGGACCCAACCGAACTG
CACGGCCGCACCGAAAACAACCGCGTGGTCAACTTCGCGCTGCCGGGCGTGCCGCAGGCCCAGCGCGACCGCATGATCGG
CCAGATGGTCGACGTGAACGTCACGCAGGCATTCCCGCATTCGCTGCGCGGCGAGTTCGTCGTGCGCCAGTAA

Upstream 100 bases:

>100_bases
CGACGCCGAAGTGAAGGCCTGGAAGGCCGCGCACTGACGCATTTTCGATTCAACCTGTTGTGCCCTTGATGCCCCCAGGT
AAACCACTGAGACCGGCACC

Downstream 100 bases:

>100_bases
GCGCCGTTCAACAAGGCTCCATGAAGATTCCAACCGCCGAATTCGTCGCTCCCAGGGACGACAACACGCGCCTGCAGAAC
CTGTGCGGGCCGCTCGACGA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKKVFVKTYGCQMNEYDSDKMVDVLNATQGMEPTDNVEDADVILFNTCSIREKAQEKVFSELGRMKALKAAKPDLVIGVG
GCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLINRRQQTGLSQVDISFPEIEKFDHLPPARVDGPSAFVSIMEGCSKYC
SYCVVPYTRGEEVSRPFEDVLAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVADIPGIERIRYTTSH
PKEFTSRLIELYGRCDKLVNHLHLPVQHASDRVLAGMKRGYSVLEYKSIIRRLRALRPDMSMSSDFIVGFPGETDADFDK
LMAMVHEIGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLRRLQLLQATIEENVQRISQSMVGTVQRILVEGPARKDPTEL
HGRTENNRVVNFALPGVPQAQRDRMIGQMVDVNVTQAFPHSLRGEFVVRQ

Sequences:

>Translated_450_residues
MKKVFVKTYGCQMNEYDSDKMVDVLNATQGMEPTDNVEDADVILFNTCSIREKAQEKVFSELGRMKALKAAKPDLVIGVG
GCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLINRRQQTGLSQVDISFPEIEKFDHLPPARVDGPSAFVSIMEGCSKYC
SYCVVPYTRGEEVSRPFEDVLAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVADIPGIERIRYTTSH
PKEFTSRLIELYGRCDKLVNHLHLPVQHASDRVLAGMKRGYSVLEYKSIIRRLRALRPDMSMSSDFIVGFPGETDADFDK
LMAMVHEIGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLRRLQLLQATIEENVQRISQSMVGTVQRILVEGPARKDPTEL
HGRTENNRVVNFALPGVPQAQRDRMIGQMVDVNVTQAFPHSLRGEFVVRQ
>Mature_450_residues
MKKVFVKTYGCQMNEYDSDKMVDVLNATQGMEPTDNVEDADVILFNTCSIREKAQEKVFSELGRMKALKAAKPDLVIGVG
GCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLINRRQQTGLSQVDISFPEIEKFDHLPPARVDGPSAFVSIMEGCSKYC
SYCVVPYTRGEEVSRPFEDVLAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVADIPGIERIRYTTSH
PKEFTSRLIELYGRCDKLVNHLHLPVQHASDRVLAGMKRGYSVLEYKSIIRRLRALRPDMSMSSDFIVGFPGETDADFDK
LMAMVHEIGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLRRLQLLQATIEENVQRISQSMVGTVQRILVEGPARKDPTEL
HGRTENNRVVNFALPGVPQAQRDRMIGQMVDVNVTQAFPHSLRGEFVVRQ

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=485, Percent_Identity=37.5257731958763, Blast_Score=308, Evalue=8e-84,
Organism=Homo sapiens, GI28872782, Length=485, Percent_Identity=37.5257731958763, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI93277076, Length=468, Percent_Identity=27.3504273504274, Blast_Score=162, Evalue=5e-40,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=62.1923937360179, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1787057, Length=427, Percent_Identity=27.8688524590164, Blast_Score=154, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=424, Percent_Identity=36.0849056603774, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI71996771, Length=313, Percent_Identity=30.0319488817891, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=32.776617954071, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI19922432, Length=462, Percent_Identity=28.3549783549784, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50152; Mature: 50152

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVFVKTYGCQMNEYDSDKMVDVLNATQGMEPTDNVEDADVILFNTCSIREKAQEKVFS
CCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
ELGRMKALKAAKPDLVIGVGGCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLINRRQQT
HHHHHHHHHCCCCCEEEECCCHHCCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHC
GLSQVDISFPEIEKFDHLPPARVDGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV
CCCEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEECCCCCCCHHCCCHHHH
LAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVADIPGIERIRYTTSH
HHHHHHHHHHCCCEEEHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHEEECCCC
PKEFTSRLIELYGRCDKLVNHLHLPVQHASDRVLAGMKRGYSVLEYKSIIRRLRALRPDM
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
SMSSDFIVGFPGETDADFDKLMAMVHEIGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLR
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHH
RLQLLQATIEENVQRISQSMVGTVQRILVEGPARKDPTELHGRTENNRVVNFALPGVPQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEECCCCCHH
QRDRMIGQMVDVNVTQAFPHSLRGEFVVRQ
HHHHHHHHHHCCCHHHHCCHHHCCCEEECC
>Mature Secondary Structure
MKKVFVKTYGCQMNEYDSDKMVDVLNATQGMEPTDNVEDADVILFNTCSIREKAQEKVFS
CCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
ELGRMKALKAAKPDLVIGVGGCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLINRRQQT
HHHHHHHHHCCCCCEEEECCCHHCCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHC
GLSQVDISFPEIEKFDHLPPARVDGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV
CCCEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEECCCCCCCHHCCCHHHH
LAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVADIPGIERIRYTTSH
HHHHHHHHHHCCCEEEHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHEEECCCC
PKEFTSRLIELYGRCDKLVNHLHLPVQHASDRVLAGMKRGYSVLEYKSIIRRLRALRPDM
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
SMSSDFIVGFPGETDADFDKLMAMVHEIGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLR
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHH
RLQLLQATIEENVQRISQSMVGTVQRILVEGPARKDPTELHGRTENNRVVNFALPGVPQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEECCCCCHH
QRDRMIGQMVDVNVTQAFPHSLRGEFVVRQ
HHHHHHHHHHCCCHHHHCCHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA